Potassium channels : methods and protocols
- edited by Show-Ling Shyng and Francis I. Valiyaveetil, Matt Whorton.
- New York, NY : Humana Press, 
- Copyright notice
- Physical description
- xvi, 345 pages : illustrations (some color) ; 27 cm.
- Springer protocols (Series)
- Methods in molecular biology (Clifton, N.J.) v. 1684.
At the library
Science Library (Li and Ma)
|QH506 .M45 V.1684||Unknown|
- Includes bibliographical references and index.
- Foreword...Preface...Table of Contents...Contributing Authors...1. Manipulating Potassium Channel Expression and Function in Hippocampal Neurons by In Utero ElectroporationKang Wang 2. Studying KCNQ1 Mutation and Drug Response in Type 1 Long QT Syndrome using Patient-Specific Induced Pluripotent Stem Cell-Derived CardiomyocytesHeming Wei, Jianjun Wu, and Zhenfeng Liu3. Monitoring Changes in the Abundance of Endogenously Expressed ATP-Sensitive Potassium (KATP) Channels in the Plasma Membrane using Surface BiotinylationJing-Syuna Ruan and Pei-Chun Chen4. Nonsense-Mediated mRNA Decay of hERG Mutations in Long QT SyndromeQiuming Gong and Zhengfeng Zhou5. Probing Subunits Interactions in KATP Channels using Photo-Crosslinking via Genetically Encoded p-azido-L-phenylalaninePrassana Devaraneni, Emily A. Rex, and Show-Ling Shyng6. Hyper-SUMOylation of K+ Channels in Sudden Unexplained Death in Epilepsy: Isolation and Primary Culture of Dissociated Hippocampal Neurons from Newborn Mice for Subcellular LocalizationXu Chen, Shanshan Zhang, Jifang Huang, Wanying Dong, Huanjie Shao, Hongmei Wu, and Yitao Qi7. Simultaneous Real-Time Measurement of the ss-Cell Membrane Potential and Ca2+ Influx to Assess the Role of Potassium Channels on ss-Cell FunctionNicholas C. Vierra, Matthew T. Dickerson, Louis H. Philipson, and David A. Jacobson8. Methods for Characterizing Disease-Associated ATP-Sensitive Potassium Channel MutationsBalamurugan Kandasamy and Show-Ling Shyng9. Thallium Flux Assay for Measuring the Activity of Monovalent Cation Channels and TransportersC. David Weaver10. Nuclear Magnetic Resonance Approaches for Characterizing Protein-Protein InteractionsYuki Toyama, Yoko Mase, Hanaho Kano, Mariko Yokogawa, Masanori Osawa, and Ichio Shimada11. Studying Mechanosensitivity of Two-Pore Domain K+ Channels in Cellular and Reconstituted Proteoliposome MembranesJosefina del Marmol, Robert A. Rietmeijer, and Stephen G. Brohawn12. Migration of PIP2 on KCNQ2 Surface Revealed by Molecular Dynamics SimulationsQiansen Zhang and Huaiyu Yang13. Studying Structural Dynamics of the Potassium Channels by Single-Molecule FRETShizhen Wang, Joshua Brettmann, and Colin G. Nichols14. Patch-Clamp Recordings of the KcsA K+ Channel in Unilamellar BlistersKimberly Matulef and Francis I. Valiyaveetil15. Combinatorial Assembly of LumitoxinsDavid Nedrud and Daniel Schmidt16. Characterization of MC4R Regulation of the Kir7.1 Channel using the Tl+ Flux AssayMichael J. Litt, Roger D. Cone, and Masoud Ghamari-Langroudi17. Stopped-Flow Fluorometric Ion Flux Assay for Ligand-Gated Ion Channel StudiesDavid J. Posson, Radda Rusinova, Olaf S. Andersen, and Crina M. Nimigean18. In Vivo Analysis of Potassium Channelopathies: Loose Patch Recording of Purkinje Cell Firing in Living, Awake ZebrafishJui-Yi Hsieh and Diane M. Papazian19. Site-Directed Unnatural Amino Acid Mutagenesis to Investigate Potassium Channel Pharmacology in Xenopus laevis OocytesRobin Y. Kim and Harley T. Kurata20. Random Spherically Constrained Single-Particle (RSC) Method to Study Voltage-Gated Ion ChannelsLiguo Wang21. CW-EPR Spectroscopy and Site-Directed Spin Labeling to Study the Structural Dynamics of Ion ChannelsCholpon Tilegenova, Benjamin W. Elberson, D. Marien Cortes, and Luis G. Cuello22. Ion Binding to Transport Proteins using Isothermal Titration CalorimetryShian Liu and Steve W. Lockless23. Building Atomic Models of the Ion Channels Based on Low Resolution Electron Microscopy Maps and Homology ModelingValery Novoseletsky, Olfat Malak, Gildas Loussouarn, and Olga S. Sokolova24. Studying Kv Channels Function using Computational MethodsAudrey Deyawe, Marina A. Kasimova, Lucie Delemotte, Gildas Loussouarn, and Mounir Tarek.
- (source: Nielsen Book Data)9781493973613 20180219
- Publisher's Summary
- This volume provides readers with the latest techniques and approaches used to study K+ channels. There are a wide range of topics covered in the book, such as channel structure; K+ channels and their roles in physiology and disease; single-molecule FRET; proteins expression levels in vivo; real-time correlation of channel activity; gating; pharmacology; physiological functions; and channelopathies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Potassium Channels: Methods and Protocols is a valuable resource for all researchers who are interested in studying potassium channels.
(source: Nielsen Book Data)9781493973613 20180219
- Publication date
- Springer Protocols
- Methods in molecular biology, 1064-3745 ; 1684
- 9781493973613 hardcover
- 1493973614 hardcover
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