Automated handling of Drosophila for biological investigation
- Eric Tatt Wei Ho.
- Aug. 2013.
- Physical description
- online resource (xxi, 169 pages) : illustrations (some color)
- Ho, Eric Tatt Wei.
- Clandinin, Thomas R. (Thomas Robert), 1970- thesis advisor.
- Schnitzer, Mark Jacob, 1970- thesis advisor (primary).
- Shenoy, Krishna V. (Krishna Vaughn). thesis advisor (primary).
- Stanford University. Department of Electrical Engineering.
- Stanford University. Committee on Graduate Studies. degree grantor.
- Includes bibliographical references (p. 153-169). 127 refs.
- ["The fruit fly, Drosophila melanogaster, is a key model species for biological research. Trained humans can manipulate, inspect and dissect individual flies, but these operations are often rate-limiting bottlenecks for screening and experimentation. Here I present a high-speed, economical robot for handling non-anesthetized adult flies. Using machine vision the robot tracks a fly's thorax and gently grabs it ~400 ms after targeting. The robot can then translate and rotate the picked fly, inspect its phenotype, dissect or release it, and thereby rapidly prepare multiple flies sequentially for a wide range of experimental formats. In one illustration, the robot restrained flies and dissected the cuticle to permit two-photon imaging of neural dynamics. In another, the robot sorted flies by sex. The robot's tireless capacity for accurate, repeatable manipulations will enable experiments and biotechnology applications that would otherwise be totally infeasible, especially those requiring high-throughput capture, testing and assessment of individual fly attributes."]
- Publication date
- Submitted to the Department of Electrical Engineering and the Committee on Graduate Studies of Stanford University.
- Thesis (Ph.D.)--Stanford University, 2013.