Biophysical analysis of bacterial and viral systems : a shock tube study of bio-aerosols and a correlated AFM/NanoSIMS investigation of vaccinia virus
- Sean Damien Gates.
- May 2013.
- Physical description
- online resource (xxii, 210 pages) : illustrations (some color)
- Gates, Sean Damien.
- Hanson, Ronald. thesis advisor (primary).
- Kuhl, Ellen, 1971- thesis advisor.
- Malkin, A. J. (Alexander J.). thesis advisor.
- Stanford University. Department of Mechanical Engineering.
- Stanford University. Committee on Graduate Studies. degree grantor.
- Includes bibliographical references (p. 201-210). 73 refs.
- The work presented herein is concerned with the development of biophysical methodology designed to address pertinent questions regarding the behavior and structure of select pathogenic agents. Two distinct studies are documented: a shock tube analysis of endospore-laden bio-aerosols and a correlated AFM/NanoSIMS study of the structure of vaccinia virus. An experimental method was formulated to analyze the biological and morphological response of endospores to gas dynamic shock waves. A novel laser diagnostic system was implemented to provide time resolved data concerning the structural decomposition of endospores in shock-heated flows. In addition, an ex situ methodology combining viability analysis, flow cytometry and scanning electron microscopy was employed to both assess the biological response of the endospore to the shock event, as well as to provide complementary data regarding the structural state of the treated endospore. This methodology was implemented to model the shock wave induced response of a variety of Bacillus endospores. The results are subsequently synthesized into a theoretical framework to be employed in modeling the interaction of endospore-laden bio-aerosols with blast waves. An analytical method combining atomic force microscopy (AFM) and nanometer-scale secondary ion mass spectrometry (NanoSIMS) was developed to examine the spatial localization and depth distribution of specific biological elements in viral systems. This methodology was implemented to analyze the distribution of 13C labeled fatty acids as well as 15N labeled thymidine in individual nanometer sized vaccinia viral particles. Based upon the 13C and 15N signals, three-dimensional depth-resolved data regarding the architecture and localization of the virion lipid membrane and the nucleoprotein complex was generated. In addition, this methodology was employed to provide direct correlation of architectural and chemical data for isolated sub-viral structures. The technique and results presented herein represent a novel tool for the structural and chemical study of both intact viral particles as well as specifically targeted sub-viral elements.
- Bacillus > physiology
- High-Energy Shock Waves
- Hot Temperature
- Bacteriological Techniques
- Flow Cytometry
- Spores, Bacterial > physiology
- Time Factors
- Vaccinia virus > physiology
- Publication date
- Submitted to the Department of Mechanical Engineering and the Committee on Graduate Studies of Stanford University.
- Thesis (Ph.D.)--Stanford University, 2013.