GLO-Roots [electronic resource] : An imaging platform enabling multidimensional characterization of soil-grown root systems
- Washington, D.C. : United States. Dept. of Energy. Office of Science ; Oak Ridge, Tenn. : distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2015
- Physical description
- 1 online resource (26 p. ) : digital, PDF file.
- United States. Dept. of Energy. Office of Science. Sponsor
- United States. Dept. of Energy. Office of Scientific and Technical Information. Distributor
- Geng, Yu Author
- Vogel, John P. Author
- Rellan-Alvarez, Ruben Author
- Lobet, Guillaume Author
- Lindner, Heike Author
- Pradier, Pierre -Luc Author
- Sebastian, Jose Author
- Yee, Muh -Ching Author
- Joint Genome Institute, Walnut Creek, CA Author
- Trontin, Charlotte Author
- LaRue, Therese Author
- Schrager-Lavelle, Amanda Author
- Haney, Cara H. Author
- Nieu, Rita Author
- Maloof, Julin Author
- Dinneny, Jose R. Author
- Carnegie Institution for Science, Stanford, CA (United States) Researcher
- Root systems develop different root types that individually sense cues from their local environment and integrate this information with systemic signals. This complex multi-dimensional amalgam of inputs enables continuous adjustment of root growth rates, direction, and metabolic activity that define a dynamic physical network. Current methods for analyzing root biology balance physiological relevance with imaging capability. To bridge this divide, we developed an integrated-imaging system called Growth and Luminescence Observatory for Roots (GLO-Roots) that uses luminescence-based reporters to enable studies of root architecture and gene expression patterns in soil-grown, light-shielded roots. We have developed image analysis algorithms that allow the spatial integration of soil properties, gene expression, and root system architecture traits. Here, we propose GLO-Roots as a system that has great utility in presenting environmental stimuli to roots in ways that evoke natural adaptive responses and in providing tools for studying the multi-dimensional nature of such processes.
- Publication date
- Published through SciTech Connect.
- eLife 4 ISSN 2050-084X AM
- Geng, Yu; Vogel, John P.; Rellan-Alvarez, Ruben; Lobet, Guillaume; Lindner, Heike; Pradier, Pierre -Luc; Sebastian, Jose; Yee, Muh -Ching; Joint Genome Institute, Walnut Creek, CA; Trontin, Charlotte; LaRue, Therese; Schrager-Lavelle, Amanda; Haney, Cara H.; Nieu, Rita; Maloof, Julin; Dinneny, Jose R.
- Carnegie Institution for Science, Stanford, CA (United States)
- Funding Information