Nucleic acid encoding a self-assembling split-fluorescent protein system [electronic resource]

Imprint: Washington, D.C. : United States. Dept. of Energy. ; Oak Ridge, Tenn. : distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2015

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Creators/Contributors

Contributor: United States. Dept. of Energy. Sponsor; United States. Dept. of Energy. Office of Scientific and Technical Information. Distributor; Waldo, Geoffrey Author; Cabantous, Stephanie Author; Los Alamos National Security, LLC, Los Alamos, NM (United States) Researcher

Contents/Summary

Summary: The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

Subjects

Subjects: Applied Life Sciences.; Basic Biological Sciences.

Bibliographic information

Publication date: 2015
Note: Published through SciTech Connect.; 07/14/2015.; "9,081,014"; "14/166,770"; Waldo, Geoffrey; Cabantous, Stephanie.; Los Alamos National Security, LLC, Los Alamos, NM (United States)
Funding information: FG02-98ER62647

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