Flower development : methods and protocols
QH506 .M45 V.1110
- Unknown QH506 .M45 V.1110
- Includes bibliographical references and index.
- Part I: Review and Overview Chapters 1. Flower Development in Arabidopsis - There is More to it than Learning your ABCs Nathanael Prunet and Thomas Jack 2. Flower Development in the Asterid Lineage Barry Causier and Brendan Davies 3. Grass Flower Development Hiro-Yuki Hirano, Wakana Tanaka, and Taiyo Toriba 4. Flower Diversity and Angiosperm Diversification Pamela S. Soltis and Douglas E. Soltis 5. Flower Development - Open Questions and Future Directions Frank Wellmer, John L. Bowman, Brendan Davies, Cristina Ferrandiz, Jennifer C. Fletcher, Robert G. Franks, Emmanuelle Graciet, Veronica Gregis, Toshiro Ito, Thomas P. Jack, Yuling Jiao, Martin M. Kater, Hong Ma, Elliot M. Meyerowitz, Nathanael Prunet, Jose Luis Riechmann PART II: Genetic and Phenotypic Analyses 6. Genetic Screens for Floral Mutants in Arabidopsis thaliana: Enhancers and Suppressors Thanh Theresa Dinh, Elizabeth Luscher, Shaofang Li, Xigang Liu, So Youn Won, and Xuemei Chen 7. Genetic and Phenotypic Analysis of Shoot Apical and Floral Meristem Development Mona M. Monfared and Jennifer C. Fletcher 8. Genetic and Phenotypic Analyses of Petal Development in Arabidopsis Judit Szecsi, Barbara Wippermann and Mohammed Bendahmane 9. Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsisand Rice Fang Chang, Zaibao Zhang, Yue Jin, and Hong Ma 10. Molecular Cell Biology of Male Meiotic Chromosomes and Isolation of Male Meiocytes in Arabidopsis thaliana Yingxiang Wang, Zhihao Cheng, Pingli Lu, Ljudmilla Timofejeva, Hong Ma 11. Genetic and Phenotypic Analyses of Carpel Development in Arabidopsis Vicente Balanza, Patricia Ballester, Monica Colombo, Chloe Fourquin, Irene Martinez-Fernandez, and Cristina Ferrandiz Part III: Microscopy and Histology 12. Microscopic Analysis of Arabidopsis Ovules Balaji Enugutti and Kay Schneitz 13. Scanning Electron Microscopy Analysis of Floral Development Robert G. Franks 14. Detection of mRNA Expression Patterns by Non-radioactive in situ Hybridization on Histological Sections of Floral Tissue Anna Medzihradszky, Kay Schneitz, and Jan U. Lohmann 15. The GUS Reporter System in Flower Development Studies Janaki S. Mudunkothge and Beth A. Krizek Part IV: Experimental Systems 16. A Floral Induction System for the Study of Early Arabidopsis Flower Development Diarmuid O'Maoileidigh and Frank Wellmer 17. Fluorescence Activated Cell Sorting of Shoot Apical Meristem Cell Types G. Venugopala Reddy 18. Translating Ribosome Affinity Purification (TRAP) for Cell-specific Translation Profiling in Developing Flowers Ying Wang and Yuling Jiao 19. Laser-assisted Microdissection Applied to Floral Tissues Samuel E. Wuest and Ueli Grossniklaus PartV: Molecular Biology, Genomics, and Systems Biology 20. Identification of Arabidopsis Knock-out Lines for Genes of Interest Jose Tomas Matus, Thilia Ferrier, and Jose Luis Riechmann 21. Gene Expression Analysis by Quantitative Real-time PCR for Floral Tissues Mariana Bustamante, Jian Jin, Oriol Casagran, Tania Nolan, and Jose Luis Riechmann 22. Misexpression Approaches for the Manipulation of Flower Development Yifeng Xu, Eng-Seng Gan, and Toshiro Ito 23. Next Generation Sequencing Applied to Flower Development: RNA-Seq Jun He and Yuling Jiao 24. Next-Generation Sequencing Applied to Flower Development: ChIP-Seq Emmanuelle Graciet, Diarmuid Seosamh O'Maoileidigh, and Frank Wellmer 25. Live-Imaging of the Arabidopsis Inflorescence MeristemMarcus G. Heisler and Carolyn Ohno 26. Gene Regulatory Network Models for Floral Organ Determination Eugenio Azpeitia, Jose Davila-Velderrain, Carlos Villarreal, and Elena R. Alvarez-Buylla.
- (source: Nielsen Book Data)
- Publisher's Summary
- In Flower Development: Methods and Protocols, researchers in the field detail protocols for experimental approaches that are currently used to study the formation of flowers, from genetic methods and phenotypic analyses, to genome-wide experiments, modeling, and system-wide approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls Authoritative and practical, Flower Development: Methods and Protocols is an essential guide for plant developmental biologists, from the novice to the experienced researcher, and for those considering venturing into the field.
(source: Nielsen Book Data)
- Publication date
- edited by José Luis Riechmann, Frank Wellmer.
- Methods in Molecular Biology, 1064-3745 ; 1110
- Springer protocols
- 978-1-4614-9408-9 (eBook)