Pluripotent stem cells : methods and protocols
- Includes bibliographical references and index.
- Sources and derivation of human embryonic stem cells / Michal Amit -- New chemical approach to the efficient generation of mouse embryonic stem cells / Hossein Baharvand and Seyedeh-Nafiseh Hassani -- Review of the methods for human iPSC derivation / Nasir Malik and Mahendra S. Rao -- Generation of human-induced pluripotent stem cells by lentiviral transduction / Jennifer C. Moore -- Generation of induced pluripotent stem cells with CytoTune, a non-integrating sendai virus / Pauline T. Lieu ... [et al.] -- Generation of human-induced pluripotent stem cells (hiPSCs) using episomal vectors on defined essential 8TM medium conditions / Andrew Fontes ... [et al.] -- Feeder-free substrates for pluripotent stem cell culture / Alexandria Sams and Mark J. Powers -- Methods for culturing human embryonic stem cells on feeders / Jasmeet Kaur and Mary Lynn Tilkins -- Methods for culturing human embryonic stem cells in a xeno-free system / Jasmeet Kaur ... [et al.] -- Directed differentiation of human pluripotent stem cells along the pancreatic endocrine lineage / Dennis Van Hoof and Muluye E. Liku -- Directed differentiation of pluripotent stem cells to functional hepatocytes / Philip Roelandt, Jolien Vanhove, and Catherine Verfaillie -- Highly efficient directed differentiation of human induced pluripotent stem cells into cardiomyocytes / Paul W. Burridge and Elias T. Zambidis -- Generation of transgene-free iPSC lines from human normal and neoplastic blood cells using episomal vectors / Kejin Hu and Igor Slukvin -- Cellular characterization of human pluripotent stem cells / Rene H. Quintanilla Jr. -- TaqMan® OpenArray® high-throughput transcriptional analysis of human embryonic and induced pluripotent stem cells / Sunali N. Patel ... [et al.] -- Epigenome analysis of pluripotent stem cells / Christopher L. Ricupero, Mavis R. Swerdel, and Ronald P. Hart -- Single cell gene expression analysis of pluripotent stem cells / Ronald V. Abruzzese and Richard A. Fekete -- Profiling stem cells using quantitative PCR protein assays / David Ruff and Pauline T. Lieu -- Labeled stem cells as disease models and in drug discovery / Catharina Ellerström, Raimund Strehl, and Johan Hyllner -- Cloning technologies / Andrew Fontes -- Stable transfection using episomal vectors to create modified human embryonic stem cells / Ying Liu, Kate Judd, and Uma Lakshmipathy -- Site-specific integration in human ESC using Jump-In (TM) TI(TM) technology / Chad C. MacArthur.
- Part I. Generation of Pluripotent Stem Cells 1. Sources and Derivation of Human Embryonic Stem Cells Michal Amit 2. A New Chemical Approach to the Efficient Generation of Mouse Embryonic Stem Cells Hossein Baharvand and Seyedeh-Nafiseh Hassani 3. A Review of the Methods for Human iPSC Derivation Nasir Malik and Mahendra S. Rao 4. Generation of Human Induced Pluripotent Stem Cells by Lentiviral Transduction Jennifer C. Moore 5. Generation of Induced Pluripotent Stem Cells with CytoTune, a Non-Integrating Sendai Virus Pauline T. Lieu, Andrew Fontes, Mohan C. Vemuri, and Chad C. MacArthur 6. Generation of Human Induced Pluripotent Stem Cells (hiPSCs) using Episomal Vectors on Defined Essential 8TM Medium Conditions Andrew Fontes, Chad C. MacArthur, Pauline T. Lieu, and Mohan C. Vemuri 7. Feeder-free Substrates for Pluripotent Stem Cell Culture Alexandria Sams and Mark J. Powers Part II. Culture and Differentiation of Pluripotent Stem Cells 8. Methods for Culturing Human Embryonic Stem Cells on Feeders Jasmeet Kaur and Mary Lynn Tilkins 9. Methods for Culturing Human Embryonic Stem Cells in a Xeno-Free System Jasmeet Kaur, Mary Lynn Tilkins, and Shayne Boucher 10. Directed Differentiation of Human Pluripotent Stem Cells Along the Pancreatic Endocrine Lineage Dennis Van Hoof and Muluye E. Liku 11. Directed Differentiation of Pluripotent Stem Cells to Functional Hepatocytes Philip Roelandt, Jolien Vanhove, and Catherine Verfaillie 12. Highly Efficient Directed Differentiation of Human Induced Pluripotent Stem Cells into Cardiomyocytes Paul W. Burridge and Elias T. Zambidis 13. Generation of iPSC Lines from Human Normal and Neoplastic Blood Cells Using Episomal Vectors Kejin Hu and Igor Slukvin Part III. Pluripotent Stem Cell Characterization 14. Cellular Characterization of Human Pluripotent Stem Cells Rene H. Quintanilla Jr. 15. TaqMan(R) OpenArray(R) High Throughput Transcriptional Analysis of Human Embryonic and Induced Pluripotent Stem Cells Sunali N. Patel, Yalei Wu, Yun Bao, Ricardo Mancebo, Janice Au-Young, and Elena Grigorenko 16. Epigenome Analysis of Pluripotent Stem Cells Christopher L. Ricupero, Mavis R. Swerdel, and Ronald P. Hart 17. Single Cell Gene Expression Analysis of Pluripotent Stem Cells Ronald V. Abruzzese and Richard A. Fekete 18. Profiling Stem Cells Using Quantitative PCR Protein Assays David Ruff and Pauline Lieu Part IV. Pluripotent Stem Cell Modification and Applications 19. Labeled Stem Cells as Disease Models and in Drug Discovery Catharina Ellerstrom, Raimund Strehl, and Johan Hyllner 20. Cloning Technologies Andrew Fontes 21. Stable Transfection Using Episomal Vectors to Create Modified Human Embryonic Stem Cells Yin Liu, Kate Wagner, and Uma Lakshmipathy 22. Site-Specific Integration in Human ESC Using Jump-In(t) TI(t) Technology Chad C. MacArthur.
- (source: Nielsen Book Data)9781627033473 20160612
- Publisher's Summary
- Human pluripotent stem cells such as human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) with their unique developmental plasticity hold immense potential as cellular models for drug discovery and in regenerative medicine as a source for cell replacement. While hESC are derived from a developing embryo, iPSC are generated with forced expression of key transcription factors to convert adult somatic cells to ESC-like cells, a process termed reprogramming. Using iPSC overcomes ethical issues concerning the use of developing embryos and it can be generated from patient-specific or disease-specific cells for downstream applications. Pluripotent Stem Cells: Methods and Protocols highlights the best methods and systems for the entire work flow. Divided into four convenient sections, topics include a focus on producing iPSC from diverse somatic sources, media systems for expanding ESC and iPSC with detailed protocols for directed differentiation into specific lineages, commonly used cellular and molecular characterization methods , and the potential application of labeled stem cells with specific methods for cloning, gene delivery and cell engineering. Written in the successful Methods in Molecular Biology(t) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Pluripotent Stem Cells: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential cellular feature.
(source: Nielsen Book Data)9781627033473 20160612
- Publication date
- Methods in molecular biology, 1064-3745 ; 997
- Springer protocols
- 1627033475 (alk. paper)
- 9781627033473 (alk. paper)
- 9781627033480 (eBook)
- 1627033483 (electronic bk.)
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