Antigen processing : methods and protocols
- Responsibility
- edited by Peter van Endert.
- Imprint
- New York : Humana Press, c2013.
- Physical description
- xvii, 589 p. : ill. (some col.) ; 26 cm.
- Series
- Methods in molecular biology (Clifton, N.J.) ; v. 960.
- Springer protocols (Series)
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| Call number | Status |
|---|---|
| QH506 .M45 V.960 |
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Creators/Contributors
- Contributor
- Endert, Peter van.
Contents/Summary
- Bibliography
- Includes bibliographical references and index.
- Contents
-
- Purification of large cytosolic proteases for in vitro assays: 20S and 26S proteasomes
- Analysis of proteasome generated antigenic peptides by mass spectrometry
- Using protease inhibitors in antigen presentation assays
- Kinetic studies of cytoplasmic antigen processing and production of MHC class I ligands
- Assaying peptide translocation by the peptide transporter TAP
- In vitro reconstitution of the MHC class I peptide-loading complex
- Studying mhc class i peptide loading and exchange in vitro
- Measuring synthesis and degradation of MHC class I molecules
- Studying ubiquitination of MHC class I molecules
- Evaluation of viral interference with MHC class I-restricted antigen processing and presentation using a flow cytometry-based approach
- Determining the activity of the transporter associated with antigen processing in the compartments of the secretory pathway
- Biochemical large-scale identification of MHC class I ligands
- Establishing MHC class I peptide motifs
- Quantitating MHC class I ligand production and presentation using TCR-like antibodies
- Biochemical analysis of naturally processed antigenic peptides presented by MHC class I molecules
- Identifying source proteins for MHC class I-presented peptides
- Purification, preparation, and use of chaperone-peptide complexes for tumor immunotherapy
- Recombinant poxviruses: Versatile tools for immunological assays
- Bioinformatics identification of antigenic peptide: Predicting the specificity of major MHC class I and II pathway players
- Evaluating CD8(+) T cell responses in vitro
- Cloning CD8(+) cytolytic T lymphocytes
- Production of CD4(+) and CD8 (+) T cell hybridomas
- Tracking antigen-specific CD8(+) T cells using MHC class I multimers
- The purification of large numbers of antigen presenting dendritic cells from mouse spleen
- Preparation of dendritic cells by in vitro cultures
- Monitoring dendritic cell activation and maturation
- Monitoring the intracellular routing of internalized antigens by immunofluorescence microscopy
- Isolation of a specialized, antigen-loaded early endosomal subpopulation by flow cytometry
- Preparing antigens suitable for cross-presentation assays in vitro and in vivo
- Gene transduction in human monocyte-derived dendritic cells using lentiviral vectors
- Pulse-chase analysis for studies of MHC class II biosynthesis, maturation, and peptide loading
- Assembly of matched [alpha/beta] subunits to HLA class II peptide receptors
- Studying MHC class II peptide loading and editing in vitro
- Study of the allogeneic response induced by endothelial cells expressing hla class ii after lentiviral transduction
- Antigen processing for mhc presentation via macroautophagy
- Studying MHC class II transport in dendritic cells
- In vitro digestion with proteases producing MHC class II ligands
- MHC-II ubiquitination
- Studying MHC class II presentation of immobilized antigen by B lymphocytes
- Production of primary human CD4(+) T cell lines and clones
- Analyzing antigen recognition by natural killer T cells
- Chromatofocusing purification of CD1B-antigen complexes and their analysis by isoelectric focusing.
- Publisher's Summary
- This 'Methods in Molecular Biology' book offers a comprehensive set of protocols for studying the presentation of antigens produced in the standard processing pathways for MHC class I and class II molecules.
(source: Nielsen Book Data)9781627032179 20160611
Subjects
Bibliographic information
- Publication date
- 2012
- Series
- Methods in molecular biology, 1064-3745 ; 960
- Springer protocols, 1949-2448
- ISBN
- 9781627032179 (alk. paper)
- 1627032177 (alk. paper)
- 9781627032186 (eBook)
- 1627032185 (eBook)
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