- 1 Isolation of Lipid Rafts from Human Neutrophils by Density Gradient CentrifugationCarl Fortin and Tamas Fulop 2 Flow Cytometry Analysis of NK Cell Phenotype and Function in Ageing Raquel Tarazona, Carmen Campos, Alejandra Pera, Beatriz Sanchez-Correa and Rafael Solana 3 Flow Cytometric Identification of Fibrocytes in the Human CirculationXinyuan Hu, Erin M. DeBiasi, and Erica L. Herzog 4 Experimental Approaches to Tissue Injury and Repair in Advanced AgeAleah L. Brubaker, Stewart R. Carter, and Elizabeth J. Kovacs 5 Multicolor Digital Flow Cytometry Used in Human Translational ImmunologySamit Joshi, Subhasis Mohanty, Albert C. Shaw 6 Flow Cytometry-Based Methods to Characterize Immune Senescence in Nonhuman PrimatesChristine Meyer, Kristen Haberthur, Mark Asquith and Ilhem Messaoudi 7 Multiparameter Phenotyping of Human PBMCs Using Mass CytometryMichael D. Leipold, Evan W. Newall, and Holden T. Maecker 8 Imaging ImmunosenescenceFeng Qian and Ruth R. Montgomery 9 Activation-induced Cytidine Deaminase and Switched Memory B Cells as Predictors of Effective In Vivo Responses to the Influenza VaccineDaniela Frasca, Alain Diaz, and Bonnie B. Blomberg 10 Analyzing the Effects of Aging on CD8+ T Cell Phenotype Using Flow CytometryMin Sun Shin and Insoo Kang 11 Cell-Mediated Immune Response to Influenza Using Ex Vivo Stimulation and Assays of Cytokine and Granzyme B ResponsesJanet C. McElhaney and Beth Gentleman 12 Assays for Monitoring Macroautophagy Activity in T CellsYair Botbol and Fernando Macian 13 Fluorescence-Based Approaches for Quantitative Assessment of Protein Carbonylation, Protein Disulfides and Protein Conformation in Biological TissuesAshish R. Chauduri, Rochelle Wei, Arunabh Bhattacharya, and Ryan Hamilton 14 Monitoring the DNA Damage Response at Dysfunctional TelomeresRekha Rai and Sandy Chang 15 Single-Cell Analysis of T Cell Receptor ab RepertoirePradyot Dash, George C. Wang, and Paul G. Thomas 16 Assessment of B Cell Repertoire in HumansYu-Chang Wu, David Kipling, and Deborah Dunn-Walters 17 Laboratory and Data Analysis Methods for Characterization of Human B Cell Repertoires by High-throughput DNA SequencingChen Wang, Yi Liu, Krishna M. Roskin, Katherine J.L. Jackson, Scott C. Boyd 18 Discovery of Novel MicroRNAs in Aging C. ElegansAlexandre de Lencastre and Frank Slack 19 Analysis of DNA Methylation by PyrosequencingColin Delaney, Sanjay K. Garg, and Raymond Yung.
- (source: Nielsen Book Data)
In this volume, expert researchers detail protocols for evaluating the protean effects of immunosenescence on innate and adaptive immune function, including approaches to the analysis of antigen receptor repertoire. In addition, Immunosenecence: Methods and Protocols guides readers through cell biology and biochemical methods for analyses of telomere dysfunction, autophagy, protein oxidation, microRNAs and DNA methylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Immunosenecence: Methods and Protocols provides a mixture of basic and advanced protocols that will be useful for immunologists in general and investigators in aging biology in particular.
(source: Nielsen Book Data)
