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1. Estudio y modelación matemática del cambio metabólico de las bacterias responsables de la eliminación biológica de fósforo en el tratamiento de aguas residuales

  • ACEVEDO JUÁREZ, BRENDA
Subjects
PAO, GAO, EBPR, SBR, Fósforo, Fosfatos, Polifosfatos, Glucógeno, PHA, VFA, Metabolismo, Modelo metabólico, Cambio metabólico, Recuperación de fósoro, PAO Tipo I, PAO Tipo II, Corto plazo, Largo plazo, Estequiometría, Cinética, Monod, and TECNOLOGIA DEL MEDIO AMBIENTE
Abstract
[EN] Phosphorus is very important in life because it plays an essential role in biological processes. The main use of phosphorus is in the fertilizer industry in the form of phosphates. These phosphates come mainly from phosphate rocks which might be exhausted in 50-100 years. The overexploitation of phosphate rocks has resulted in decreased quality of reserves, and it has raised the cost of extraction, processing and shipping. Moreover, phosphorus coming from wastewater, phosphate rock dissolution, and soil with an excessive supply of fertilizer, is deposited on the surface water bodies causing a serious pollution problem called eutrophication. One of the systems most used to reduced phosphorus levels in the wastewater is the enhanced biological phosphorus removal (EBPR). This process involves capturing biologically, alternating between anaerobic oxic/anoxic conditions, the wastewater phosphorus through the phosphorus accumulating organisms (PAOs). However, one of the main problems of this process is that the glycogen accumulating organisms (GAOs) compete with PAOs for volatile fatty acids (VFA). Even though there have been many studies on the factors affecting competition between PAOs and GAOs, there are still many unanswered questions regarding the metabolism of PAOs when they lack energy reserves in the form of intracellular polyphosphates (poly-P) and its effect on the population dynamics of PAOs and GAOs in an activated sludge system. Therefore, the main goal of this dissertation is to study short- and long-term the metabolic behavior of the PAOs to different levels of poly-P; to analyze the population dynamics of microorganisms involved in the process EBPR; to model mathematically that metabolic behavior; and finally, to evaluate the possible recovery of phosphorus by extracting poly-P present in the PAOs. In the short- and long-term study was observed a metabolic shift correlates with the content in poly-P so that under low contents of poly-P the PAOs are able to behave as GAOs but without a significant development of the GAO population. Although, in both studies was observed the same metabolic behavior, from the microbiological point of view were observed some differences. In the short-term, the PAO Type II clearly showed the metabolic shift, while long-term were the PAO Type I. From the experiments performed, necessary expressions (stoichiometric and kinetic) were developed to include new behaviors observed (metabolic rate) in metabolic models existing today. Monod type expressions were developed and implemented on the model of the PAOs to represent the change between the typical stoichiometric parameters of PAO and GAO metabolism. The model was calibrated and validated showing the ability to correctly represent the metabolic change of PAOs under low concentrations of poly-P. When was observed that with low concentrations of poly-P the PAOs have the ability to change its metabolism, without the process was deteriorated by the development of the GAO population, two operating strategies were evaluated to obtain a stream rich in phosphorus to allow later retrieval. The strategies studied differed in the level of extraction of the poly-P from PAOs. In the first strategy, it was extracted less than 40 % of poly-P, while the second strategy, it came to extract more than 90 % of poly-P. The second strategy showed a higher extraction efficiency, achieving recover up to 81 % of the phosphorus present in the wastewater. As a result, of work performed four articles were generated, three of them published in journals of particular importance (2 in the journal Water Research and 1 in the journal Chemical Engineering Journal) constituting the main body of this thesis.

2. Estudio y modelación matemática del cambio metabólico de las bacterias responsables de la eliminación biológica de fósforo en el tratamiento de aguas residuales

Subjects
PAO, GAO, EBPR, SBR, Fósforo, Fosfatos, Polifosfatos, Glucógeno, PHA, VFA, Metabolismo, Modelo metabólico, Cambio metabólico, Recuperación de fósoro, PAO Tipo I, PAO Tipo II, Corto plazo, Largo plazo, Estequiometría, Cinética, Monod, and TECNOLOGIA DEL MEDIO AMBIENTE

3. Effect of a total substitution of vegetable protein and phosphates on shrinkage by cooking and purging in chopped york ham. [2020]

  • Alfonso Cardona-Hincapié, José, Alonso Restrepo-Molina, Diego, and Humberto López-Vargas, Jairo
  • Revista Facultad Nacional de Agronomía Medellin. 2020, Vol. 73 Issue 3, p9333-9340. 8p.
Subjects
VEGETABLES, MEAT, HAM, HEAT treatment, PHOSPHATES, PORK, and POLYPHOSPHATES
Abstract
The trend with the most significant impact on food is currently clean labeling, and meat products are not exempt from it. This trend promotes the elimination of additives of inorganic origin and their replacement by natural ingredients in the formulation of products. In the present work, the effects of the total substitution of polyphosphate and vegetable protein for citric fiber and hydrolyzed pork collagen in chopped pork York ham, with an extension of 52.9% at the end of cooking, were evaluated to achieve clean labeling. Two treatments were performed with two types of brine, which had a citrus fiber A and a citrus fiber B as phosphate replacements. Additionally, as a vegetable protein replacement, the same hydrolyzed pork collagen was used for both treatments. Tumbler massaging was made to allow correcting protein extraction, then it was subjected to heat treatment by immersion in hot water at 80 °C. It was concluded that the ham made with citric fiber B and hydrolyzed pork collagen obtained better results in texture, syneresis, sensory analysis and cooking losses, with no significant differences with the standard. [ABSTRACT FROM AUTHOR]

4. Utility of bone replacement material in cochlear implant, skull base and sinus surgery: Our experience [2010]

  • Artal Sánchez, Raquel, Royo López, Juan, Urpegui García, Angel, Alfonso Collado, José Ignacio, and Vallés Varela, Héctor
  • In Acta Otorrinolaringologica (English Edition) 2010 61(6):428-433

5. Utilidad del material sustitutivo óseo en la cirugía del implante coclear, base de cráneo y senos paranasales: nuestra experiencia [2010]

  • Artal Sánchez, Raquel, Royo López, Juan, Urpegui García, Angel, Alfonso Collado, José Ignacio, and Vallés Varela, Héctor
  • In Acta Otorrinolaringologica Espanola January 2010

6. Improvement of frozen crab technology with use of ultrasonic treatment / Совершенствование технологии мороженого краба с использованием ультразвуковой обработки [2015]

  • Kim, Eh.N., Lapteva, E.P., and Poroshin, N.A., Far Eastern State Technical Fishery Univ., Vladivostok
  • News of institutes of higher education. Food technology, 2015, no. 1, p. 37-42.
Subjects
Food processing and preservation, RUSSIAN FEDERATION, FEDERATION DE RUSSIE, CONTENIDO VITAMINICO, CANGREJO DE MAR, POLYPHOSPHATES, CRABE, TENEUR EN VITAMINES, PHOSPHORE, VITAMIN CONTENT, MUSCLE FIBRES, NONPROTEIN NITROGEN, NITROGENO NO PROTEICO, FOSFORO, POLIFOSFATOS, FIBRE MUSCULAIRE, OXYDE, ULTRASONIC TREATMENT, POLYPHOSPHATE, YIELDS, CAPACIDAD DE RETENCION DE AGUA, TRATAMIENTO CON ULTRASONIDO, RENDIMIENTO, VALEUR NUTRITIVE, RENDEMENT, WATER HOLDING CAPACITY, CAPACITE DE RETENTION D'EAU, FIBRAS MUSCULARES, AZOTE NON PROTEIQUE, TRAITEMENT AUX ULTRASONS, PHOSPHORUS, OXIDES, NUTRITIVE VALUE, CRABS, OXIDOS, VALOR NUTRITIVO, and FEDERACION DE RUSIA
Abstract
Food additives on the basis of polyphosphates were used to reduce losses of valuable nutrients in the manufacture of frozen products from the Far Eastern species of crab. Processing by ultrasound (US) is used for acceleration of their diffusion in muscular tissue of crab. Process of saturation of muscular tissue of crab with polyphosphates was studied. After cutting the extremity of the Far Eastern crab was placed in bathtubs with sodium polyphosphate solution in 8, 10 or 12% concentration and processed with US of 2-6 W/sq.cm intensity for 10, 20 or 30 min. It is experimentally found that as a result of processing of the Far Eastern crab extremities with US the speed of saturation of muscular tissue phosphorus oxide increases by 2-5 times. Joint impact of US and polyphosphates on maintenance of water-holding capacity of Far Eastern crab's muscle tissue extremities and prevention of losses of vitamin B12 and non-protein nitrogen by them during thermal processing of raw materials is shown. The found statistical dependences are described by the mathematical equations on which basis rational parameters of US processing in polyphosphates solutions of several marketable species of Far Eastern crab are calculated. The developed technology is approved under production conditions. On the biological value of meat and its safety the developed prototypes didn't concede to the control. Introduction of the offered technology allows to accelerate saturation process of muscular tissue of crab with phosphorus oxide by 18-20 times and increase the yield of crab's frozen extremities by 4-5%.
Для снижения потерь ценных питательных веществ при производстве мороженой продукции из дальневосточных видов краба применяются пищевые добавки на основе полифосфатов. Для ускорения их диффузии в мышечную ткань краба используют обработку ультразвуком (УЗ). Изучали процесс насыщения мышечной ткани краба полифосфатами. После разделки конечности камчатского краба помещали в ванны с раствором натрийполифосфата в концентрации 8, 10 или 12% и обрабатывали УЗ интенсивностью 2-6 Вт/кв.см в течение 10, 20 или 30 мин. Экспериментально установлено, что в результате обработки конечностей камчатского краба УЗ скорость насыщения мышечной ткани оксидом фосфора увеличивается в 2-5 раз. Показано совместное воздействие УЗ и полифосфатов на сохранение водоудерживающей способности мышечной ткани конечностей камчатского краба и предотвращение потерь ими витамина В12 и небелкового азота при тепловой обработке сырья. Установленные статистические зависимости описаны математическими уравнениями, на основе которых рассчитаны рациональные параметры обработки УЗ в растворах полифосфатов нескольких промысловых видов дальневосточного краба. Разработанная технология апробирована в производственных условиях. По биологической ценности мяса и его безопасности выработанные опытные образцы не уступали контрольным. Внедрение предлагаемой технологии позволяет ускорить процесс насыщения мышечной ткани краба оксидом фосфора в 18-20 раз и увеличить выход мороженых конечностей краба на 4-5%.

7. Development and evaluation of protective capacity of Salmonella Enteritidis polyphosphate kinase-deleted and temperature-sensitive mutant [2013]

  • Kim, K., Kangwon National University, Chuncheon, Republic of Korea, Park, S., Kangwon National University, Chuncheon, Republic of Korea, Cho, Y., Kangwon National University, Chuncheon, Republic of Korea, Kwak, J.Y., Kangwon National University, Chuncheon, Republic of Korea, Kang, Z.W., Kangwon National University, Chuncheon, Republic of Korea, Kim, E.H., ChoongAng Vaccine Lab., DaeJeon, Republic of Korea, Choi, H.W., ChoongAng Vaccine Lab., DaeJeon, Republic of Korea, Won, H.K., ChoongAng Vaccine Lab., DaeJeon, Republic of Korea, Noh, Y.H., ChoongAng Vaccine Lab., DaeJeon, Republic of Korea, and Hahn, T.W.
  • Korean Journal of Veterinary Research, December 2013, v. 53(4) p. 211-216.
Subjects
cross protection, polyphosphate kinase deleted mutant, temperature-sensitive mutant, Veterinary science and hygiene, POLYPHOSPHATES, POLIFOSFATOS, POLYPHOSPHATE, and SALMONELLA ENTERITIDIS
Abstract
This study was focusing on evaluating the protection of polyphosphate kinase (ppk) deleted and/or temperature-sensitive (ts) Salmonella Enteritidis (SE) as an attenuated vaccine in chickens. We constructed SEppk, SEts and SEppk::ts mutants and screened those mutants by growth capability in vitro, protection study in mice model and antibody response in chickens. Among the mutants, SEppk::ts-3 was selected because it showed higher growth capability, good protection against highly virulent SE in mice model, and good antibody response in chickens. SEppk::ts-3 also showed good protection against highly virulent SE isolate because it decreased colonization of virulent SE challenge strain in spleen, liver and cecum compared with the non-vaccinated control. The SEppk::ts-3 mutant showed crossprotection against S. Gallinarum (SG) challenge although the its cross-protection rate was a little lower than that of SG9R, a commercial vaccine against SG infection. To use for live attenuated vaccine in chickens, it should further be characterized.

8. Efecto de la carencia de polifosfatos inorgánicos en la expresión global de proteínas y en los determinantes de resistencia a cobre en el arqueón sulfolobus solfataricus [2015]

  • Soto Vergara, Daniela Fernanda
Subjects
Polifosfatos, Proteínas, Cobre, and Arquebacterias
Abstract
Memoria para optar el título de Bioquímico
Mientras los mecanismos de resistencia al cobre han sido ampliamente estudiados en bacterias, se tiene escasa información sobre los mecanismos de resistencia al cobre en arqueas. Recientemente se ha identificado un grupo de genes (cop) de resistencia al cobre, altamente conservado en las arqueas del orden Sulfolobales. Este grupo de genes incluye un regulador transcripcional específico de arqueas (copR), una metalochaperona de cobre (copT) y un transportador de cationes ATPasa tipo P (copA). Otro posible mecanismo de resistencia al cobre en Sulfolobales comprendería a los polifosfatos (poliP). Los poliP son moléculas lineales presentes en todos los seres vivos y que cumplen diversas funciones celulares. El modelo que vincula los poliP con la resistencia a cobre propone una disminución de poliP mediada por la exopolifosfatasa (PPX), enzima que degradaría el gran polímero hasta fosfato inorgánico (Pi), el cual podría unirse a cobre para ser finalmente expulsado hacia el periplasma o al medio extracelular por transportadores específicos. Recientemente nuestro laboratorio ha logrado generar una cepa de Sulfolobus solfataricus recombinante con niveles deficientes de poliP, con el fin de evaluar el efecto de su carencia en distintos fenómenos, entre ellos la resistencia a cobre. Basados en estudios transcripcionales realizados mediante la técnica PCR en tiempo real, determinamos que la inducción de la expresión de copA en respuesta a cobre ocurre más tempranamente en células carentes de poliP, comparadas con células que poseen este polímero. Una explicación a este fenómeno sería que en ausencia de poliP, el modelo que vincula los poliP con el eflujo de cobre no sería operativo. En consecuencia, el sistema cop debería ser activado más tempranamente para compensar la carencia de poliP.Con el objetivo de profundizar en el conocimiento que se tiene sobre los poliP y su relación con la resistencia a cobre en S. solfataricus, analizamos la expresión diferencial de proteínas en células carentes de poliP y en células expuestas a cobre. De esta forma, mediante la técnica de proteómica cuantitativa ICPL determinamos que en carencia de poliP las células de S. solfataricus sobreexpresaron proteínas involucradas en la degradación de glucógeno y ácidos grasos, junto a aquellas que remueven especies reactivas del oxígeno (ROS). Por otro lado, algunas de las proteínas que disminuyeron sus niveles en carencia de poliP están involucradas en la gluconeogénesis y el ciclo del glioxilato. Estos resultados en conjunto, sugieren que los poliP podrían desempeñar un papel energético en S. solfataricus, ya que al parecer, en su ausencia se favorecen las vías catabólicas. La sobreexpresión de proteínas que remueven ROS sugiere un mayor estado de estrés oxidativo en las células S. solfataricus poliP-. Adicionalmente, los experimentos de proteómica cuantitativa nos permitieron determinar que en presencia de cobre, las células de S. solfataricus poliP+ sobreexpresan proteínas involucradas en el ciclo del TCA, en la degradación y plegamiento de proteínas, la reparación del ADN y en la degradación del ARN. Estos resultados sugieren que la presencia de cobre podría provocar daño a tanto a nivel de proteínas, como de ácidos nucleicos en las células de S. solfataricus. El aumento en los niveles de algunas proteínas involucradas en el ciclo del TCA puede relacionarse con el ATP y el poder reductor que las células necesitan para enfrentar una situación de estrés (ej: proteólisis, plegamiento de proteínas). Finalmente, las células carentes de poliP y expuestas a cobre aumentaron los niveles de algunas proteasas, de proteínas que forman parte de la cadena transportadora de electrones y de proteínas que participan en la remoción de ROS, como la peroxiredoxina y la superóxido dismutasa (SOD). Si bien la peroxiredoxina se aumentó sus niveles en todas las condiciones de estudio, la SOD sólo lo hizo cuando las células de S. solfataricus carentes de poliP, fueron expuestas a cobre. En conjunto estos resultados sugieren un mayor nivel de estrés oxidativo en las células S. solfataricus poliP- expuestas a cobre y un papel energético de los poliP. Dentro de las proteínas que disminuyeron sus niveles, encontramos proteínas ribosomales. Esto podría incidir directamente en la síntesis proteica, disminuyendo la capacidad de las células de S. solfataricus poliP- de hacer frente a una situación de estrés
While copper resistance mechanisms have been widely studied in bacteria, there is little information about copper resistance mechanisms of Archaea. Recently, a highly conserved group of genes (cop) related to the resistance to copper was identified in some species of Sulfolobales. This group of genes consists of an archaeal-specific transcriptional regulator (copR), a putative metallochaperone (copT) and a P-type cation-transporting ATPase (copA). Another potential mechanism involved in copper resistance of Sulfolobales would involve inorganic polyphosphates (polyP). PolyP is a linear polymer present in all organisms and performs a wide variety of functions. The model that links polyP with resistance to copper suggests that the exopolyphosphatase (PPX) mediates the degradation of polyP to inorganic phosphate (Pi) which could eventually join copper and be finally ejected to the periplasm or to the extracellular medium by specific transporters. Recently, our laboratory has generated a recombinant Sulfolobus solfataricus strain deficient in polyP levels, in order to evaluate the role of its deficiency in various phenomena, including copper resistance. Based on transcriptional studies carried out through real-time PCR, we determined that the induction of the expression of copA in response to copper occurs earlier in cells lacking polyP, than those possessing the polymer. One explanation for this phenomenon could be that, in the absence of polyP, the model linking polyP to copper efflux would not be operational. As a consequence, the cop system should be activated earlier to compensate for the lack of polyP. To gain insight into polyP and its relationship to copper resistance in S. solfataricus, we studied the differential expression of proteins in cells lacking polyP and in cells exposed to copper. Thus, quantitative proteomics studies (ICPL) showed that in the absence of polyP S. solfataricus cells overexpresses proteins involved in: the degradation of glycogen and fatty acids, and in the removal of reactive oxygen species (ROS). On the other hand, some of the proteins that decreased their levels are involved in gluconeogenesis and the glyoxylate cycle. These results suggest that polyP could play an energetic role in the cell considering that the lack of this polymer, upregulated proteins involved in catabolic pathaways. The upregulation of proteins involved in removal of ROS suggests the presence of a higher level of oxidative stress in S.solfataricus poliP- cells. Further, ICPL experimentas allowed us to determine that in the presence of copper, S. solfataricus polyP+ cells overexpress proteins involved in: the TCA cycle, the degradation and folding of proteins, DNA repairing, and in RNA degradation. These results suggest that the presence of copper could cause damage to both proteins and nucleic acids from S. solfataricus cells. The up-regulation of some TCA proteins could be related with the ATP levels and reducing power that cells need to face a stress situation (e.g. proteolysis, folding of proteins). Finally, when cells lacking polyP were exposed to copper, there was an overexpression of proteases, electron transport chain proteins, and proteins involved in ROS removal (peroxiredoxin and SOD). Although peroxiredoxin was overexpress in all our study conditions, SOD was only up-regulated when S. solfataricus polyP- cells were exposing to copper. These findings may reflect a higher oxidative stress in cells lacking polyP and also suggest an energetic role of polyP. Some of the proteins that decreased their levels were ribosomal proteins. This could directly affect protein synthesis and therefor the ability of S. solfataricus polyP- cells to respond to a stressing situation

9. Construction of plant-based expression vector of polyphosphate kinase gene / μø(PPK)ֲĹ [2012]

  • Cao Fang, Huzhou Teachers College, Huzhou, Yang Zhihong, Huzhou Teachers College, Huzhou, and Han Zhiping, Huzhou Teachers College, Huzhou
  • Agricultural Science and Technology, Oct. 2012, v. 13(10) p.2073-2075,2079.
Subjects
Plant genetics and breeding, ESCHERICHIA COLI, POLYPHOSPHATES, GENES, VECTORS, POLYPHOSPHATE, GENE, VECTEUR DE MALADIE, POLIFOSFATOS, and VECTORES
Abstract
Resume (Zh,En)
14 ref.
[Ŀ]ںϱPPKɫӫ⵰׵ںϱpCAMBIA1302-PPK[]GenBankе¼Ĵ󳦸˾PPKУL03719E.coli DH5DNAΪģ壬ͨPCRPPKȻIn-Fusion@ HD Cloning KitPPK¡pCAMBIA1302Nco Iøλ㡣[]вⶨʾpCAMBIA1302-PPKԼ2.0 kbPPKƬΣ˵PPKѾֲpCAMBIA1302ɫӫ⵰׻ǰ[]ɹںϱPPKɫӫ⵰׵ںϱpCAMBIA1302-PPK
[Objective] The aim was to construct the fusion expression vector of polyphosphate kinase (PPK) and green fluorescent protein (GFP) genes. [Method] In this study, the primers were designed based on PPK gene sequence (L03719) of E. coli DH5 in Genbank. Genomic DNA of E. coli DH 5 was extracted as template for the amplification of PPK gene by PCR method. By using In-Fusion@ HD Cloning Kit, the PPK gene was directionally cloned into NcoI site of the pCAMBIA1302 vector. [Result] Sequencing results showed that the 2.0 kb long fragment of PPK gene was inserted into the plant-based expression vector pCAMBIA1302 in front of GFP gene. [Conclusion] The fusion expression vector of PPK and GFP genes were successfully constructed.

10. Effect of additives on sensory quality of domestic meat products / Vpliv dodatkov na senzoricno kakovost domacih mesnih izdelkov [2014]

  • 23rd International Scientific Symposium on Nutrition of Farm Animals 'Zadravec-Erjavec Days', , Radenci, 13-14 Nov 2014, Murn, M., Ljubljana Univ. (Slovenia). Biotechnical Fac., Zootechnical Dept. , Enota za prasicerejo., Zemva, M., Kos, I., Ceh, T., Malensek, M., Kovac, M., and Malovrh, S.
Subjects
Food additives, PROPRIETE ORGANOLEPTIQUE, POLYPHOSPHATES, CALIDAD, NITRITOS, NITRITES, NITRITE, ADITIVOS ALIMENTARIOS, COMMON SALT, PRODUCTOS DE LA CARNE, POLIFOSFATOS, POLYPHOSPHATE, ANALYSE ORGANOLEPTIQUE, FOOD ADDITIVES, MEAT PRODUCTS, PRODUIT CARNE, ORGANOLEPTIC ANALYSIS, QUALITY, SAL, ORGANOLEPTIC PROPERTIES, PROPIEDADES ORGANOLEPTICAS, ADDITIF ALIMENTAIRE, SEL DE CUISINE, QUALITE, and ANALISIS ORGANOLEPTICO
Abstract
The purpose of this paper was to determine if randomly selected consumers perceive differences between domestic meat products with greater or lesser contents of nitrites, added polyphosphate and salt. Sensory quality was evaluated to 12 dry sausages, 8 dry pork neck, 7 sausages for cooking and 6 bratwursts. The content of nitrites, added polyphosphate and salt were determined in labs. Appearance,

11. Changes in the ultrastructure of lamb muscles as influenced by freezing and sodium tripolyphosphate (STPP) [2011]

  • Abdel-Aal, H. A., Minia University, El-Minia and Mohamed, H. M. A.
  • Lucrari stiintifice. Seria Zootehnie - Universitatea de Stiinte Agricole si Medicina Veterinara Ion Ionescu de la Brad (Romania), 2011, vol. 56(16) p. 286-293.
Subjects
Food processing and preservation, Food composition, LAMB MEAT, FROZEN MEAT, POLYPHOSPHATES, MUSCLES, ULTRASTRUCTURE, WATER HOLDING CAPACITY, VIANDE D'AGNEAU, VIANDE CONGELEE, POLYPHOSPHATE, MUSCLE, CAPACITE DE RETENTION D'EAU, CARNE DE CORDERO, CARNE CONGELADA, POLIFOSFATOS, MUSCULOS, ULTRAESTRUCTURA, and CAPACIDAD DE RETENCION DE AGUA
Abstract
Summary (En)
Three lamb muscles from shoulder, Supraspinatus (SS); lion, Longissimus dorsi (LD) and leg, Biceps femoris (BF) were kept in refrigerator at 4C for 24 hr, dipped in 10% sodium tripolyphosphate (STPP) solution for 1 min and frozen at _18C or at _ 40C for 48 hr. Proximate composition, phosphorus, drip loss, cooking loss, pH and water holding capacity (WHC) were determined. Ultrastructure of treated and untreated muscles was studied using transmission electron microscopy (TEM). No significant (p0.05) differences were found between moisture, protein and fat content of the three lamb muscles. Muscles treated with STPP had higher phosphorus content than that of untreated muscles. Treated or untreated frozen lamb muscles at _ 40C had lower drip losses and cooking losses than those frozen at _18C. All three lamb muscles treated with STPP had lower drip losses and cooking losses and higher pH and WHC than those of untreated ones. Results of the study suggested that STPP increased the pH, improved the WHC and decreased drip loss and cooking loss of frozen lamb muscles. Freezing at _40C and STPP treatment maintained the well-defined all sarcomere components of lamb muscles compared to untreated muscles and frozen at _18C.

12. Avaliação antibiofilme e anticariogênica de nanocompósitos formados por polifosfatos de sódio e flúor associados à nanopartículas de prata : estudo in vitro [2018]

  • Gouvêa, Carla Corrêa Mendes [UNESP], Universidade Estadual Paulista (UNESP), Danelon, Marcelle [UNESP], Amaral, Jackeline Gallo do, and Barbosa, Debora de Barros [UNESP]
  • Repositório Institucional da UNESP
    Universidade Estadual Paulista
Subjects
Nanopartículas de prata, Polifosfatos, Biofilme, Cárie dentária, Desmineralização do dente, and Polyphosphates
Abstract
Submitted by Carla Corrêa Mendes Gouvêa (carla_cmendes@hotmail.com) on 2018-06-07T14:56:34Z No. of bitstreams: 1 Tese corrigida Carla Correa Mendes Gouvêa.pdf: 2438125 bytes, checksum: 89f73cd540d3d9b9bbdf46167a4ca374 (MD5) Approved for entry into archive by Ana Paula Rimoli de Oliveira null (anapaula@foa.unesp.br) on 2018-06-07T18:14:52Z (GMT) No. of bitstreams: 1 gouvea_ccm_dr_araca_int.pdf: 2438125 bytes, checksum: 89f73cd540d3d9b9bbdf46167a4ca374 (MD5) Made available in DSpace on 2018-06-07T18:14:52Z (GMT). No. of bitstreams: 1 gouvea_ccm_dr_araca_int.pdf: 2438125 bytes, checksum: 89f73cd540d3d9b9bbdf46167a4ca374 (MD5) Previous issue date: 2018-05-04 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) O objetivo deste estudo foi sintetizar e caracterizar nanocompósitos formados por polifosfatos (trimetafosfato de sódio (TMP) ou hexametafosfato de sódio (HMP)) e flúor (F) associados à nanopartículas de prata. Esses nanocompósitos foram avaliados quanto à sua ação anti-biofilme de Candida albicans (ATCC 10231) e Streptococcus mutans (ATCC 25175) e seu potencial em inibir a desmineralização do esmalte dentário por meio de ensaio in vitro de ciclagem de pH. As nanopartículas de prata foram sintetizadas através da redução do nitrato de prata (1 ou 10%) pelo borihidreto de sódio (NaBH4) em meio isopropílico contendo trimetafosfato de sódio (TMP) ou hexametafosfato de sódio (HMP)) e flúor (F). Os nanocompósitos foram caracterizados por microscopia eletrônica de varredura (MEV) e por difração de raios-X, e também determinada a concentração inibitória mínima (MIC) contra células planctônicas de C. albicans e S. mutans. A efetividade contra biofilmes pré-formados de 24h dos nanocompósitos, foi testada em concentrações de 1x e 10x os valores de MIC (de 40μg Ag ml−1), avaliada através da quantificação de células cultiváveis (CFUs), da atividade metabólica (XTT) e da biomassa total (Cristal Violeta). Os nanocompósitos contendo 10% de Ag apresentaram maior efetividade antimicrobiana contra ambos os microrganismos, comparados aos nanocompósitos contendo 1% de prata. O biofilme de S. mutans foi mais suscetível aos nanocompósitos TMP ou HMP/AgNP que o biofilme de C. albicans, com reduções respectivas de 2,88-3,71 log10 e 0,45-1,43 log10. Os nanocompósitos permitiram uma diminuição de cerca de 75% da biomassa total e em torno de 90% da atividade metabólica em ambos os biofilmes. Para avaliação in vitro do potencial anticárie, foi sintetizado um novo nanocompósito contendo 0,2% de TMP, 100 ppm de flúor e 10% de Ag e caracterizado por microscopia eletrônica de transmissão (MET). Blocos de esmalte bovino (4 mm x 4 mm, n = 60) selecionados pela dureza superficial inicial (SHi) foram alocados em cinco grupos (n = 12): água deionizada (Placebo), 100 ppm F (100F), 225 ppm F (225F), 100 ppm F + 0,2% TMP (100F/TMP) e 100 ppm F + 0,2% TMP + 10% Ag (100F/TMP/Ag). Os blocos foram tratados 2x ao dia com as soluções e submetidos a cinco ciclos de pH (soluções des e remineralizadora) à 37° C. Em seguida, determinou-se a dureza superficial final (SHf) e a dureza subsuperficial integrada (ΔKHN), e a concentração de fluoreto (F) e cálcio (Ca) no esmalte. A efetividade desse nanocompósisto (100F/TMP/Ag) contra a formação de biofilmes simples e misto de C. albicans (ATCC 10231) e S. mutans (ATCC 25175) foi avaliada por meio da contagem de unidades formadoras de colônias (CFUs). Os biofilmes foram também analisados por microscopia eletrônica de varredura (MEV). A dureza superficial (% SH) foi similar nas amostras tratadas com soluções contendo 225F, 100F/TMP e 100F/TMP/Ag (p> 0,001). Além disso, a capacidade de reduzir o corpo da lesão (ΔKHN) foi maior em 110F, 225F, 100F/TMP e 100F/TMP/Ag (p <0,001) na área A (5-20 μm) e na área B somente com 225F e 100F/TMP. O grupo 100F/ TMP/Ag apresentou maior desmineralização na área B (20-130 μm) (p <0,001). A concentração de F da solução 100F/TMP/Ag foi semelhante a 225F (p <0,001). Os grupos 100F, 225F, 100F/TMP e 100F/TMP/Ag apresentaram a maior e similar concentração de Ca (p> 0,001). A dureza superficial (% SH) foi similar nas amostras tratadas com soluções contendo 225F, 100F / TMP e 100F / TMP / Ag (p> 0,001). Além disso, a capacidade de reduzir o corpo da lesão (ΔKHN) foi maior em 110F, 225F, 100F / TMP e 100F / TMP / Ag (p <0,001) na zona A (5-20 μm) e na zona B apenas com 225F e 100F / TMP. O grupo 100F / TMP / Ag apresentou maior desmineralização na zona B (20-130 μm) (p <0,001). A concentração de F da solução 100F / TMP / Ag foi semelhante a 225F (p <0,001). Os grupos 100F, 225F, 100F / TMP e 100F / TMP / Ag apresentaram a maior e a mesma concentração de Ca (p> 0,001).Embora a solução 100F/TMP/Ag tenha apresentado valores mais baixos de áreas profundas do que a solução contendo 225 ppm F, o nanocompósito foi capaz de inibir a desmineralização da superfície do esmalte. Estes nanocompósitos demonstraram atividade antimicrobiana significativa, especialmente contra S. mutans, e podem ser considerados uma alternativa potencial para novos biomateriais odontológicos, visando a prevenção ou tratamento de lesões cariosas. The aim of this study was to synthesize and characterize nanocomposites formed polyphosphates (sodium trimetaphosphate (TMP) or sodium hexametaphosphate (HMP)) and fluoride (F) associated with silv er nanoparticles. The anti - biofilm activity of the nanocomposites were evaluated against Candida albicans (ATCC ATCC 10231) and Streptococcus mutans (ATCC 25175) . as well as their potential in inhibiting demineralization and increasing the remineralizatio n of dental enamel by in vitro pH cycling assay. Silver nanoparticles were synthesized by reduction of silver nitrate (1 or 10%) by sodium bor ohydride (NaBH 4 ) in isopropylic medium containing sodium trimetaphosphate (TMP) or sodium hexametap hosphate (HMP)) and fluoride (F) . The nanocomposites were characterized by scanning electron microscopy (SEM) and X - ray diffraction. The minimum inhibitory concentration (MIC) of the nanocomposites was determined against planktonic cells of C. albicans and S. mutans . Eff ect iveness of preformed 24 - hour biofilms of nanocomposites at 1x and 10x MIC values was assessed by quantifying viable cells (CFUs), metabolic activity (XTT) and total biomass (Crystal Violet). The nanocomposites containing 10% Ag showed higher antimicrobia l effectiveness against both microorganisms compared to nanocomposites containing 1% silver. The S. mutans biofilm was more susceptible to the TMP or HMP - AgNP nanocomposites than the C. albicans biofilm, with respective reductions of 2.88 - 3.71 log 10 and 0. 45 - 1.43 log 10 . The nanocomposites allowed a reduction of about 75% of the total biomass and about 90% of the metabolic activity in both biofilms. For the in vitro evaluation of the anticaries potential, a new nanocomposite containing 0.2% TMP, 100 ppm fluo rine and 10% Ag was synthesized and characterized by transmission electron microscopy (TEM). B ovine enamel blocks (4 mm x 4 mm, n = 60) selected by initial surface hardness (SHi) were allocated in five groups (n=12): deionized water (Placebo ), 100 ppm F (1 00 F), 225 ppm F (225 F), 100 ppm F + 0.2% TMP (100 F / TMP) and 100 ppm F + 0.2% TMP + 10% Ag (100F / TMP / Ag). The blocks were treated 2x daily with the solutions and subjected to five pH cycles (deismineralizang and remineralizing solutions) at 37 ° C. Then, the final surface hardness (SHf) and integrated subsurface hardness ( Δ KHN), the concentration of fluoride (F) and calcium (Ca) in the enamel was determined. The effectiveness of this nanocomposite (100F / TMP / Ag) against the formation of single an d mixed biofilms of C. albicans (ATCC 10231) and S . mutans (ATCC 25175) was evaluated by the count ing colony forming units (CFUs). Biofilms were also analyzed by scanning electron microscopy (SEM). Similar values of % SH wer e observed for groups 225F, 100F /TMP and 100F/TMP Ag (p> 0.001). The 225F, 100F/TMP and 100F/TMP/Ag solutions showed capacity to reduce the lesion body (ΔKHN) in the depth of 5 - 20 μm. F concentration was similar in the enamel for the 100F/TMP/Ag and 225F (p> 0.001). The groups 100F, 225F, 100F/TMP and 100F/TMP/Ag presented the highest and the same concentration of Ca (p> 0.001). In relation to viable cells 100F/TMP/Ag nanocomposite promoted significant log reductions in the number of CFUs of S. mutans , of 5.42 and 4.46 log 10 respectivel y in single and mixed biofilms and C. albicans was mor e resistant. SEM images confirmed these results. The surface hardness (% SH) was similar in the samples treated with solutions containing 225F, 100F/TMP and 100F/TMP/ Ag (p> 0.001). In addition, the abil ity to reduce the lesion body (ΔKHN) was greater in 110F, 225F, 100F / TMP and 100F/TMP/ Ag (p <0.001) in zone A (5 - 20 μm) and in zone B only with 225F and 100F/TMP. The 100F/TMP/ Ag group showed greater demineralization in zone B (20 - 130 μm) (p <0.001). The F concentration of the 100F /TMP/ Ag solution was similar to 225F (p <0. 001). Groups 100F, 225F, 100F/ TMP and 100F /TMP/ Ag showed the highest and the same concentration of Ca (p> 0.001).Although the 100F/TMP/Ag solution yielded lower values of lesion deep ar eas than the solution containing 225 ppm F, but the nanocomposite was able to inhibit demineralization of surface of enamel. These compounds have demonstrated significant antimicrobial activity, especially against S. mutans , and can be considered a potenti al alternative for new dental bio materials , aiming at the prevention or treatment of carious lesions .

13. Influence of the pH in the stability of the silver coacervate by vitreous precursors [2018]

  • Montanari, Bianca, Vast, Pierre, Martines, Marco Antonio Utrera, Ribeiro, Sidney José Lima, Messaddeq, Younes, and Universidade Estadual Paulista (UNESP)
  • Eclética Química, Vol 27, Iss spe, Pp 305-314 (2002)
    Fundação Editora da Universidade Estadual Paulista Júlio de Mesquita Filho
    Eclética Química, Volume: 27, Issue: spe, Pages: 305-314, Published: 2002
    Eclética Química v.27 n.spe 2002
    Repositório Institucional da UFMS
    Repositório Institucional da UNESP
    Eclética Química, Vol 27, Iss 2 (2018)
    Universidade Estadual Paulista
    Universidade Federal de Mato Grosso do Sul
Subjects
Chemistry, vidros e fosfatos, Glass, coacervate, glasses and phosphates, coacervato, QD1-999, Phosphates, Fosfatos, Polyphosphates, Polifosfatos, and Vidro
Abstract
Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:49:11Z No. of bitstreams: 0 Made available in DSpace on 2013-08-22T18:49:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2002-01-01 Made available in DSpace on 2013-09-30T19:10:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2002-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T14:20:38Z No. of bitstreams: 0 Made available in DSpace on 2014-05-20T14:20:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2002-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Neste trabalho, usando o processo de coacervação, foi estudado o sistema NaPO3/AgNO3/H2O. Determinou-se o domínio de coacervação a partir de diferentes concentrações de NaPO3 e AgNO3 e a influência do pH na estabilização do coacervato foi determinada. Os coacervatos obtidos em pH ~ 5 apresentam maior região de estabilidade. In this work, using coacervation process, the influence of the pH in the stability of the coacervates of the NaPO3/AgNO3/H2O system was studied. The coacervate domain was established using different concentrations of NaPO3 and AgNO3 and the influence of the pH in the stability of the coacervate was putfoward. The coacervates obtained at pH ~ 5 present highest stability. UNESP Instituto de Química Departamento de Química Geral e Inorgânica UNESP Instituto de Química Departamento de Química Geral e Inorgânica

14. Bacterial phosphate metabolism and its application to phosphorus recovery and industrial bioprocesses [2010]

  • Hirota, R., Hiroshima Univ., Higashihiroshima, Kuroda, A., Kato, J., and Ohtake, H.
  • Journal of Bioscience and Bioengineering (Japan), May 2010, v. 109(5) p. 423-432.
Subjects
Processing of agricultural wastes, WASTES, PHOSPHATES, METABOLISM, RECYCLING, FERTILIZER INDUSTRY, MICROORGANISMS, POLYPHOSPHATES, DECHET, PHOSPHATE, METABOLISME, RECYCLAGE DES DECHETS, INDUSTRIE DES ENGRAIS, MICRO-ORGANISME, POLYPHOSPHATE, DESECHOS, FOSFATOS, METABOLISMO, RECICLAJE, INDUSTRIA DE ABONOS, MICROORGANISMOS, and POLIFOSFATOS
Abstract
Summary (En)
7 fig. 66 ref.
Enhanced biological phosphorus removal (EBPR) has become a well-established process and is currently applied in many full-scale wastewater treatment processes. Phosphorus recovered from EBPR waste sludge can be used as a raw material for the fertilizer industry, if a sound recycling strategy is developed and applied. In this review, we summarize our current knowledge on phosphate metabolism in bacteria, focusing on molecular mechanisms of bacterial polyphosphate (polyP) accumulation. A simple method for releasing polyP from EBPR waste sludge and recovering phosphorus in a reusable form for the fertilizer industry is presented. We also describe a recent development of bioprocesses for the expanded use of polyP in the production of value-added chemicals.

15. Electron microscopic analysis of heat-induced leakage of polyphosphate from a phoU mutant of Escherichia coli [2010]

  • Kashihara, H., Osaka Univ., Suita, Kang, B.M., Omasa, T., Honda, K., Sameshima, Y., Kuroda, A., and Ohtake, H.
  • Bioscience, Biotechnology, and Biochemistry (Japan), Apr 2010, v. 74(4) p. 865-868.
Subjects
Processing of agricultural wastes, ESCHERICHIA COLI, MUTANTS, MICROSCOPY, POLYPHOSPHATES, HEAT TREATMENT, X RAYS, CYTOPLASM, MUTANT, MICROSCOPIE, POLYPHOSPHATE, TRAITEMENT THERMIQUE, RAYON X, CYTOPLASME, MUTANTES, MICROSCOPIA, POLIFOSFATOS, TRATAMIENTO TERMICO, RAYOS-X, and CITOPLASMA
Abstract
Summary (En)
3 fig. 18 ref.

16. The content of nitrate and polyphosphates in meat products from zhe processing of farms / Vsebnost nitritov in polifosfatov v mesnih izdelkih iz predelave na kmetijah [2013]

  • 22th International Scientific Symposium on Nutrition of Farm Animals 'Zadravec-Erjavec Days', , Radenci, 14-15 Nov 2013, Murn, M., Ljubljana Univ. (Slovenia). Biotechnical Fac., Zootechnical Dept., Enota za prasicerejo., Kovac, M., Zemva, M., Kos, I., Ceh, T., Malensek, M., and Malovrh, S.
  • Proceedings of the 22nd International Scientific Symposium on Nutrition of Farm Animals 'Zadravec-Erjavec Days', Ceh, T.; Kapun S..- Murska Sobota (Slovenia): Kmetijsko gozdarska zbornica Slovenije, Murska Sobota (Slovenia);Kmetijsko gozdarski zavod, Murska Sobota (Slovenia), 2013. 22th International Scientific Symposium on Nutrition of Farm Animals 'Zadravec-Erjavec Days', Radenci, 14-15 Nov 2013
Subjects
Food additives, MEAT PRODUCTS, ADDITIVES, NITRITES, POLYPHOSPHATES, FOOD INDUSTRY, FAMILY FARMS, PRODUIT CARNE, ADDITIF, NITRITE, POLYPHOSPHATE, INDUSTRIE ALIMENTAIRE, EXPLOITATION AGRICOLE FAMILIALE, PRODUCTOS DE LA CARNE, ADITIVOS, NITRITOS, POLIFOSFATOS, INDUSTRIA ALIMENTARIA, and EXPLOTACION AGRICOLA FAMILIAR
Abstract
The food industry has made great strides in technology and the use of various additives in processed meat products to ensure the integrity of these microbiologically and facilitate recovery technology. The most controversial additives in excess proven harmful to human health, so it is additive intake via food products is limited. In Slovenia there are local meat processors as an alternative to mea

17. Real-time PCR analysis of Candidatus 'Accumulibacter phosphatis' in A/O activated sludge enhancing phosphate release activity [2009]

  • Tsuji, K., Fujita, M., Akashi, A., Fukushima, T., and Furumai, H.
  • Journal of Japan Society on Water Environment (Japan), Feb 2009, v. 32(2) p. 87-91.
Subjects
Processing of agricultural wastes, Water resources and management, ACETATES (SALTS), POLYPHOSPHATES, PCR, ANALYSIS, ACTIVATED SLUDGE, ACETATE (SEL), POLYPHOSPHATE, ANALYSE, BOUE ACTIVEE, ACETATOS (SALES), POLIFOSFATOS, ANALISIS, and LODOS ACTIVADOS
Abstract
Summaries (En, Ja)
3 tab. 2 fig. 17 ref.

18. Genetic interaction between ribosome biogenesis and inositol polyphosphate metabolism in Saccharomyces cerevisiae [2009]

  • Horigome, C., Ikeda, R., Okada, T., Takenami, K., and Mizuta, K.
  • Bioscience, Biotechnology and Biochemistry (Japan), Feb 2009, v. 73(2) p. 443-446.
Subjects
Research methods, Food science and technology, INOSITOL, SACCHAROMYCES CEREVISIAE, RIBOSOMES, POLYPHOSPHATES, BIOSYNTHESIS, RIBOSOME, POLYPHOSPHATE, BIOSYNTHESE, RIBOSOMAS, POLIFOSFATOS, and BIOSINTESIS
Abstract
Summary (En)
3 fig. 19 ref.

19. Применение пищевых полифосфатных добавок при производстве продукции из гидробионтов / Приготовление бульонов из кожи, костей и голов горбуши / Using polyphosphate additives in preparing products from hydrobionts [Broth making of hunchback salmon skin, bones and heads] [2009]

  • Kim, Eh.N., Nagajtseva, N.V., Mar'yasov, V.L., and Pravdina, T.V., Far East State Technical Fishery Univ., Vladivostok
  • Treatises of Far Eastern State Technical Fisheries University(v.21, p.1), Far Eastern State Technical Fisheries University, Vladivostok (Russian Federation).- Vladivostok (Russian Federation): FESTFU, 2009.- 978-5-88871-468-3.- p.292-298
Subjects
Fisheries production, MOLLUSCA, MEAT, POLYPHOSPHATES, FREEZING, TECHNOLOGY, FOOD PROCESSING, ORGANOLEPTIC ANALYSIS, RUSSIAN FEDERATION, VIANDE, POLYPHOSPHATE, CONGELATION, TECHNOLOGIE, TRAITEMENT DES ALIMENTSTRAITEMENT DES ALIMENTS, ANALYSE ORGANOLEPTIQUE, FEDERATION DE RUSSIE, CARNE, POLIFOSFATOS, CONGELACION, TECNOLOGIA, PROCESAMIENTO DE ALIMENTOSPROCESAMIENTO DE ALIMENTOS, ANALISIS ORGANOLEPTICO, and FEDERACION DE RUSIA
Abstract
4 tables
The optimum conditions of using polyphosphate additives Peskaplus in preparing raw frozen dressed hunchback salmon were studied. There are given the characteristics of modern polyphosphate additives improving the structure of proteins of muscular fibers of hydrobionts. Exposure of hunchback salmon in iced pickle of polyphosphate additives increased the output of the semi-product before freezing by 18.3%. The following freezing and storing at -18 degrees C for 12 months promoted after defrosting increasing the water-retaining ability in trial samples compared to the control samples. The treatments of hunchback salmon with polyphosphate additives slows down the decomposition nucleotides that are important for organoleptic evaluation of the product, as well as reduces the total microbial contamination at the primary stage of processing and following refrigerating storage. Degustation of foods made from trial samples softer and more juicy consistency, improved taste, lighter color compared to the control samples were observed. The conserves made from trial samples got a higher organoleptic evaluation than conserves from control samples. It is concluded that using polyphosphate additives in manufacturing of hunchback salmon meat allows at most preserving the useful properties of feedstock, to reduce losses by the following treating and getting high organoleptic characteristics of different groups of uniform products made of hunchback salmon meat

20. Effects of anoxia on 31P NMR spectra of Phycomyces blakesleeanus during development [2009]

  • Stanic, M., University of Belgrade, Belgrade (Serbia). Faculty of Biology, Zivic, M., University of Belgrade, Belgrade (Serbia). Faculty of Biology, and Zakrzewska, J., Institute of General and Physical Chemistry Belgrade
  • Archives of Biological Sciences (Serbia), 2009, v. 61(1) p. 17-22.
Subjects
Plant physiology - Nutrition, PHYCOMYCES, ANOXIA, POLYPHOSPHATES, NMR SPECTROSCOPY, ANOXIE, POLYPHOSPHATE, SPECTROSCOPIE RMN, POLIFOSFATOS, and ESPECTROSCOPIA RMN
Abstract
Summaries (En, Sr)
3 graphs
21 ref.
The method of 31P NMR spectroscopy was used to investigate the effects of anoxia on Phycomyces blakesleeanus mycelium during development. The greatest changes were recorded in the PPc, NADH, and alfa-ATP signals. Decrease of PPc signal intensity is due to chain length reduction and reduction in number of PPn molecules. Smaller decrease of beta-ATP compared to alfa-ATP signal intensity can be attributed to maintenance of ATP concentration at the expense of PPn hydrolysis. Sensitivity to anoxia varies with the growth stage. It is greatest in 32-h and 44-h mycelium, in which PPn is used as an additional energy source, while the smallest effect was noted for 36-h fungi.

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