Yisa Song, BA, Fei Wang, MA, Yaxun Wei, MA, Dong Chen, BA, and Gang Deng, BA
Technology in Cancer Research & Treatment, Vol 20 (2021) Technology in Cancer Research & Treatment
Subjects
Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282, Cancer Research, Oncology, Original Article, ATP5A1, RNA-seq, alternative splicing, apoptosis, and cancer
Abstract
Background: Aberrant expression and alternative splicing of oncogenes are the driving events in tumor initiation and development. But how these events are regulated in cancer cells is largely unknown. Functions of ATP5A1, an important mitochondrial ATP synthase gene, in transcriptional and posttranscriptional regulation were explored in this study. Methods: ATP5A1 was overexpressed using plasmid-transformed HeLa cells, and its influence on cell apoptosis and proliferation is evaluated. Transcriptome sequencing was then performed using RNA-seq to study the changes in gene expression and regulation of alternative splicing events. Validation of the implicated genes was achieved using RT-qPCR analysis. Results: It was found that ATP5A1 could significantly promote cellular apoptosis, but it had no influence on cell proliferation. ATP5A1 overexpression significantly increased the expression levels of genes associated with the innate immune response, angiogenesis, and collagen catabolic processes. This included enrichment of MMP2 and MMP19. It was also found that ATP5A1 could interfere with the alternative splicing of hundreds of genes associated with glucose homeostasis, HIF-1 signaling activation, and several pathways associated with cancers. Eight ATP5A1-regulated differentially expressed genes and 3 genes altered by splicing were selected and validated using RT-qPCR analysis. Conclusions: In summary, we illustrate the regulatory functions of ATP5A1 on the transcriptome of HeLa cells by exploring its influence on gene expression and alternative splicing. The results suggest that ATP5A1 may play an important regulatory role in cervical cancer cells by regulating expression and alternative splicing of cancer-associated genes. This study provides novel insights into the current understanding of the mechanisms of ATP5A1 on carcinogenesis and cancer progression.
Song, BA, Yisa, Wang, MA, Fei, Wei, MA, Yaxun, Chen, BA, Dong, and Deng, BA, Gang
Technology in Cancer Research & Treatment; 9/14/2021, p1-13, 13p
Subjects
ECTOPIC tissue, GENE expression, CANCER genes, KNOTS & splices, and HELA cells
Abstract
Background: Aberrant expression and alternative splicing of oncogenes are the driving events in tumor initiation and development. But how these events are regulated in cancer cells is largely unknown. Functions of ATP5A1, an important mitochondrial ATP synthase gene, in transcriptional and posttranscriptional regulation were explored in this study. Methods: ATP5A1 was overexpressed using plasmid-transformed HeLa cells, and its influence on cell apoptosis and proliferation is evaluated. Transcriptome sequencing was then performed using RNA-seq to study the changes in gene expression and regulation of alternative splicing events. Validation of the implicated genes was achieved using RT-qPCR analysis. Results: It was found that ATP5A1 could significantly promote cellular apoptosis, but it had no influence on cell proliferation. ATP5A1 overexpression significantly increased the expression levels of genes associated with the innate immune response, angiogenesis, and collagen catabolic processes. This included enrichment of MMP2 and MMP19. It was also found that ATP5A1 could interfere with the alternative splicing of hundreds of genes associated with glucose homeostasis, HIF-1 signaling activation, and several pathways associated with cancers. Eight ATP5A1-regulated differentially expressed genes and 3 genes altered by splicing were selected and validated using RT-qPCR analysis. Conclusions: In summary, we illustrate the regulatory functions of ATP5A1 on the transcriptome of HeLa cells by exploring its influence on gene expression and alternative splicing. The results suggest that ATP5A1 may play an important regulatory role in cervical cancer cells by regulating expression and alternative splicing of cancer-associated genes. This study provides novel insights into the current understanding of the mechanisms of ATP5A1 on carcinogenesis and cancer progression. [ABSTRACT FROM AUTHOR]
Song, BA, Yisa, Wang, MA, Fei, Wei, MA, Yaxun, Chen, BA, Dong, and Deng, BA, Gang
Subjects
111299 Oncology and Carcinogenesis not elsewhere classified, FOS: Clinical medicine, 110320 Radiology and Organ Imaging, and Biochemistry
Abstract
Supplemental material, sj-docx-1-tct-10.1177_15330338211039126 for ATP5A1 Participates in Transcriptional and Posttranscriptional Regulation of Cancer-Associated Genes by Modulating Their Expression and Alternative Splicing Profiles in HeLa Cells by Yisa Song, BA, Fei Wang, MA, Yaxun Wei, MA, Dong Chen, BA, Gang Deng and BA in Technology in Cancer Research & Treatment
Affected by high switching speed and parasitic parameters, crosstalk problem of eGaN HEMT in a phase-leg configuration cannot be ignored. By decreasing gate driver turn-off voltage, the false turn-on phenomenon of device caused by crosstalk can be avoided, but it is hard to realize the minimization of total power loss. Thus, we analyze the influence of gate driver turn-off voltage on eGaN HEMT reverse conduction loss, switching loss and crosstalk voltage, and propose an optimized design rules for determining turn-off voltage value in a phase-leg configuration. By reasonably choosing gate driver turn-off voltage, a balance between optimal loss and reliable operation is achieved, thereby effectively alleviating the conflict between efficiency and reliability in the power electronic system.
