Edelstein, Arthur D, Tsuchida, Mark A, Amodaj, Nenad, Pinkard, Henry, Vale, Ronald D, and Stuurman, Nico
Journal of biological methods, vol 1, iss 2
Subjects
Micro-Manager, high-content screening, high-speed imaging, localization microscopy, and open-source microscopy
Abstract
μManager is an open-source, cross-platform desktop application, to control a wide variety of motorized microscopes, scientific cameras, stages, illuminators, and other microscope accessories. Since its inception in 2005, μManager has grown to support a wide range of microscopy hardware and is now used by thousands of researchers around the world. The application provides a mature graphical user interface and offers open programming interfaces to facilitate plugins and scripts. Here, we present a guide to using some of the recently added advanced μManager features, including hardware synchronization, simultaneous use of multiple cameras, projection of patterned light onto a specimen, live slide mapping, imaging with multi-well plates, particle localization and tracking, and high-speed imaging.
Statistics - Applications and Statistics - Computation
Abstract
Computerized touchscreen "Direct Recording Electronic" DRE voting systems have been used by over 1/3 of American voters in recent elections. In many places, insufficient DRE numbers in combination with lengthy ballots and high voter traffic have caused long lines and disenfranchised voters who left without voting. We have applied computer queuing simulation to the voting process and conclude that far more DREs, at great expense, would be needed to keep waiting times low. Alternatively, paper ballot-optical scan systems can be easily and economically scaled to prevent long lines and meet unexpected contingencies.
Gabr, Refaat E., Schär, Michael, Edelstein, Arthur D., Kraitchman, Dara L., Bottomley, Paul A., and Edelstein, William A.
Subjects
Article
Abstract
As the number of MRI phased array coil elements grows, interactions among cables connecting them to the system receiver become increasingly problematic. Fiber optic or wireless links would reduce electromagnetic interference, but their dynamic range (DR) is generally less than that of coaxial cables. Raw MRI signals, however, have a large DR because of the high signal amplitude near the center of k-space. Here, we study DR in MRI in order to determine the compatibility of MRI multicoil imaging with non-coaxial cable signal transmission. Since raw signal data are routinely discarded, we have developed an improved method for estimating the DR of MRI signals from conventional magnitude images. Our results indicate that the DR of typical surface coil signals at 3 T for human subjects is less than 88 dB, even for three-dimensional acquisition protocols. Cardiac and spine coil arrays had a maximum DR of less than 75 dB and head coil arrays less than 88 dB. The DR derived from magnitude images is in good agreement with that measured from raw data. The results suggest that current analog fiber optic links, with a spurious-free DR of 60–70 dB at 500 kHz bandwidth, are not by themselves adequate for transmitting MRI data from volume or array coils with DR ~90 dB. However, combining analog links with signal compression might make non-coaxial cable signal transmission viable.
A power driven micrometer for engaging a workpiece surface has a rotatable screw and a motor for rotatingly driving the screw. The screw and motor are secured together for longitudinal travel as a unit, the screw being threadedly engaged and supported by a nut in the micrometer casing. A key precludes rotation of the motor relative to the casing while enabling longitudinal travel of the motor relative to the casing.
An optical mount enabling independent orthogonal adjustment of the angular position of an optical element comprises a cell adapted to receive an optical element and a base defining a chamber open on one side thereof for receipt of at least a part of the cell therein. The cell comprises a circumferentially extending partial spherical section and a pair of portions extending outwardly of the spherical section, and the base comprises an angular seat on the open side receiving and conforming to at least a portion of the spherical section of the cell and a pair of portions extending outwardly of the seat and facing the cell portions. A pair of independent adjustable means spaced from each other by substantially 90.degree. are secured to one of the portion pairs for movement therewith and have parts adapted to operatively abut against aligned sections of the other of the portion pairs. The parts of the adjustable means and the corresponding aligned sections of the other portion pair are biased towards each other. The cell is pivotable orthogonally about axes passing through the geometric center thereof, without cross-coupling by independent adjustment of the adjustable means.
Columbia Companion to the Twentieth-Century American Short Story; 2000, p117-121, 5p
Subjects
COLLEGE teachers, AMERICAN short stories, AMERICAN literature, and PROSE literature
Abstract
This article presents information related to the writer John Barth who was born in 1930 and grew up on the Eastern Shore of Maryland, a circumstance, he suggests in his autobiographical essay "Some Reasons Why," that is one of the roots of his often unconventional writing style. To date, Barth has published nine novels that range in mode from the nihilistic, "The Floating Opera," and "End of the Road," to the encyclopedic, "The Sot Weed Factor," and "Giles Goat-Boy." He has taught at Pennsylvania State University, the State University of New York at Buffalo, and since 1973, at Johns Hopkins University, where he is currently professor emeritus in the Writing Seminars.
