Biomedical engineering, Génie biomédical, Otorhinolaryngology, stomatology, dentistry, Otorhinolaryngologie, stomatologie, dentisterie, Sciences biologiques et medicales, Biological and medical sciences, Sciences medicales, Medical sciences, Otorhinolaryngologie. Stomatologie, Otorhinolaryngology. Stomatology, Face, maxillaires, dents, parodonte: maladies, sémiologie, Facial bones, jaws, teeth, parodontium: diseases, semeiology, Chirurgie (generalites). Transplantations, greffes d'organes et de tissus. Pathologie des greffons, Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases, Chirurgie orl et maxillofaciale. Chirurgie dentaire. Orthodontie, Head and neck surgery. Maxillofacial surgery. Dental surgery. Orthodontics, Chirurgie maxillofaciale. Chirurgie dentaire. Orthodontie, Maxillofacial surgery. Dental surgery. Orthodontics, Chirurgie orthopédique, Orthopedic surgery, Mammalia, Primates, Stomatologie, Stomatology, Estomatología, Vertebrata, Biomatériau, Biomaterial, Calcium Phosphate, Calcium Phosphates, Calcio Fosfato, Dispositif médical, Medical device, Dispositivo médico, Parodontopathie, Periodontal disease, Parodontopatía, Phosphate de calcium, Calcium phosphate, Calcio fosfato, Singe, Monkey, Mono, Phosphate tricalcique, Régénération osseuse, Bone regeneration, Regeneración ósea, GDF-5, biological factors, bone regeneration, medical devices, periodontal disease, and periodontal regeneration
The application of growth factors has been advocated in support of periodontal regeneration. Recombinant human growth and differentiation factor-5 (rhGDF-5), a member of the bone morphogenetic protein family, has been used to encourage periodontal tissue regeneration. This study evaluated the dose response of rhGDF-5 lyophilized onto beta-tricalcium phosphate (bTCP) granules for periodontal tissue regeneration in a baboon model. Periodontal defects were created bilaterally in 12 baboons by a split-mouth design. Plaque was allowed to accumulate around wire ligatures to create chronic disease. After 2 mos, the ligatures were removed, and a notch was placed at the base of the defect. Two teeth on each side of the mouth were randomly treated with bTCP only, 0.5, 1.0, or 2.0 mg rhGDF-5/g bTCP. Animals were sacrificed 5 mos post-treatment, with micro-CT and histomorphometric analysis performed. After 5 mos, analysis showed alveolar bone, cementum, and periodontal ligament formation in all treatment groups, with a dose-dependent increase in rhGDF-5-treated groups. Height of periodontal tissues also increased with the addition of rhGDF-5, and the amount of residual graft material decreased with rhGDF-5 treatment. Therefore, rhGDF-5 delivered on bTCP demonstrated effective regeneration of all 3 tissues critical for periodontal repair.