Lone SH, Bhat KA, Naseer S, Rather RA, Khuroo MA, and Tasduq SA
Journal Of Chromatography. B, Analytical Technologies In The Biomedical And Life Sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2013 Dec 01; Vol. 940, pp. 135-41. Date of Electronic Publication: 2013 Sep 27.
Animals, Cell Line, Tumor, Flavonoids analysis, Flavonoids isolation purification, Flavonoids pharmacology, Hexanes, Humans, Limit of Detection, Linear Models, Mice, Phytosterols analysis, Phytosterols isolation purification, Phytosterols pharmacology, Plant Extracts pharmacology, Plant Roots chemistry, Plant Shoots chemistry, Reproducibility of Results, Sesquiterpenes analysis, Sesquiterpenes isolation purification, Sesquiterpenes pharmacology, Artemisia chemistry, Cell Survival drug effects, Chromatography, High Pressure Liquid methods, and Plant Extracts chemistry
Wild and tissue culture raised regenerants of Artemisia amygdalina, a critically endangered and endemic plant of Kashmir and North West Frontier Provinces of Pakistan were screened for the amount of bioactive principles and in particular antimalarial compound artemesinin. Phytochemical screening of extracts revealed the presence of terpenes, alkaloids, phenolics, tannins (polyphenolics), cardiac glycosides and steroids in wild (aerial, inflorescence) and tissue culture regenerants (in vitro grown plant, callus and green house acclimatized plants). HPLC of Artemisia amygdalina revealed the presence of artemesinin in petroleum ether extracts of wild aerial part, tissue culture raised plant and green house acclimatized plants. Acetonitrile and water in 70:30 ratios at flow rate of 1ml/min was standardised as mobile phase. Retention time for standard chromatogram was 6.7. Wild inflorescences and callus does not produce artemesinin. This is the first report of phytochemical screening and artemesinin estimation of wild and tissue culture raised regenerants of Artemisia amygdalina.
This study was conducted to analyse the free radical scavenging potential of callus obtained from nodal segments and leaf explants of Artemisia amygdalina Decne. The explants were inoculated on MS medium augmented with various concentrations of BAP, Kn, NAA and 2,4-D for callus induction. In this study, 12.42 g of callus developed from the leaf explant on MS (NAA 10 + BAP 7.5 µM) and 8.81 g of callus developed from nodal explant on NAA 2 µM+BAP 2 µM. Callus raised from both explants on all treatments seemed non-regenerative but BAP 2 µM produced 7.33 shoots and BAP 15 µM produced callus and 5 shoots per nodal segment. Callus was analysed for antioxidant activity via DPPH, riboflavin photoxidation and DNA damage assays. Methanol and aqueous extracts show more scavenging in DPPH, deoxyribose assay and in contrast, petroleum ether and ethyl acetate extracts show higher activity in riboflavin photoxidation assay. Tocopherol, ascorbic acid and BHT were used as controls.