Introduction: Non-hematopoietic stem cells may be a source of paracrine and structural regeneration for brain damaged by acute ischemic stroke. In this study, we investigated correlations of CD34-, CD34/CXCR4-, and CXCR4-positive peripheral blood CD45-negative stem cells with the neurological and functional status of 34 acute stroke patients. Material and methods: Blood was sampled and assessed by flow cytometry on days 1, 2, 4, and 6 after stroke onset. Parallel to blood sampling and after 3 and 6 months, patients were assessed with the National Institute of Health Stroke Scale, Barthel Index, Scandinavian Stroke Scale, and modified Rankin Scale. Blood was also sampled in 15 control subjects matched for age and sex, without history of previous stroke. Results: We observed very low levels of the observed stem cells resident in peripheral blood. Higher baseline numbers of all 3 stem cell types correlated with better neurological or functional status on admission. Additionally, higher increases in CD34- and CD34/CXCR4-positive stem cell number and lower increase in CXCR4-positive cells correlated with initially worse neurological status. However, increased CD34- and CD34/CXCR4-positive cell induction in patients correlated with better functional/neurological status after the 6-month follow-up. Conclusions: We report that the number and dynamic changes in levels of non-hematopoietic CD34-, CD34/CXCR4-, and CXCR4-positive stem cells tend to correlate with acute stroke patients' neurologic and functional status. [ABSTRACT FROM AUTHOR]
Clark, Barry, Wachowiak, Bartosz, W. Crawford, Ewan, Jakubowski, Zenon, and Kabata, Janusz
Journal of Analytical Methods In Chemistry; 1998, Vol. 20 Issue: 1
A pilot study was performed to evaluate the feasibility of using the Internet to securely deliver patient laboratory results, and the system has subsequently gone into routine use in Poland. The system went from design to pilot and then to live implementation within a four-month period, resulting in the LIS-Interlink software product. Test results are retrieved at regular intervals from the BioLinkTM LIS (Laboratory Information System), encrypted and transferred to a secure area on the Web server. The primary health-care centres dial into the Internet using a local-cell service provided by Polish Telecom (TP), obtain a TCP/IP address using the TP DHCP server, and perform HTTP ‘get’ and ‘post’ operations to obtain the files by secure handshaking. The data are then automatically inserted into a local SQL database (with optional printing of incoming reports)for cumulative reporting and searching functions. The local database is fully multi-user and can be accessed from different clinics within the centres by a variety of networking protocols.