Antolli, Paolo G., Liu, Zhiming, Antolli, Paolo G., and Liu, Zhiming
Bioreactors, Water--Purification--Equipment and supplies, and Tissue engineering--Equipment and supplies
Bioreactors are the heart of many biotechnological systems that are used for agriculture, environmental, industrial and medical applications. This book presents the design, properties and applications of bioreactors. Topics discussed include bioreactors for microalgae; application of different types of bioreactors in bioprocesses; airlift bioreactors and its application in fermentation and wastewater treatment; development and deployment of a bioreactor for the removal of sulfate and manganese from circumneutral coal mine drainage and kinetic coefficients and factors affecting aeration efficiency as design parameters of MBR.
Lakhveer Singh, Abu Yousuf, Durga Madhab Mahapatra, Lakhveer Singh, Abu Yousuf, and Durga Madhab Mahapatra
Bioreactors: Sustainable Design and Industrial Applications in Mitigation of GHG Emissions presents and compares the foundational concepts, state-of-the-art design and fabrication of bioreactors. Solidly based on theoretical fundamentals, the book examines various aspects of the commercially available bioreactors, such as construction and fabrication, design, modeling and simulation, development, operation, maintenance, management and target applications for biofuels production and bio-waste management. Emerging issues in commercial feasibility are explored, constraints and pathways for upscaling, and techno-economic assessment are also covered. This book provides researchers and engineers in the biofuels and waste management sectors a clear, at-a-glance understanding of the actual potential of different advanced bioreactors for their requirements. It is a must-have reference for better-informed decisions when selecting the appropriate technology models for sustainable systems development and commercialization.Focuses on sustainable bioreactor processes and applications in bioenergy and bio-waste managementExplores techno-economic and sustainability assessment aspects through a comparative approach, catering to diverse arrays and applicationsOffers comprehensive coverage of the most recent technology, from fundamentals to applications
In this expert handbook both the topics and contributors are selected so as to provide an authoritative view of possible applications for this new technology. The result is an up-to-date survey of current challenges and opportunities in the design and operation of bioreactors for high-value products in the biomedical and chemical industries. Combining theory and practice, the authors explain such leading-edge technologies as single-use bioreactors, bioreactor simulators, and soft sensor monitoring, and discuss novel applications, such as stem cell production, process development, and multi-product reactors, using case studies from academia as well as from industry. A final section addresses the latest trends, including culture media design and systems biotechnology, which are expected to have an increasing impact on bioreactor design. With its focus on cutting-edge technologies and discussions of future developments, this handbook will remain an invaluable reference for many years to come.
Maxwell BM, Birgand F, Schipper LA, Barkle G, Rivas AA, Helmers MJ, and Christianson LE
Journal of environmental management [J Environ Manage] 2020 Oct 15; Vol. 272, pp. 110996. Date of Electronic Publication: 2020 Jul 18.
New Zealand, Nitrates analysis, Selection Bias, Bioreactors, and Denitrification
Woodchip bioreactors are a practical, low-cost technology for reducing nitrate (NO 3 ) loads discharged from agriculture. Traditional methods of quantifying their performance in the field mostly rely on low-frequency, time-based (weekly to monthly sampling interval) or flow-weighted sample collection at the inlet and outlet, creating uncertainty in their performance and design by providing incomplete information on flow and water chemistry. To address this uncertainty, two field bioreactors were monitored in the US and New Zealand using high-frequency, multipoint sampling for in situ monitoring of NO 3 -N concentrations. High-frequency monitoring (sub hourly interval) at the inlet and outlet of both bioreactors revealed significant variability in volumetric removal rates and percent reduction, with percent reduction varying by up to 25 percentage points within a single flow event. Time series of inlet and outlet NO 3 showed significant lag in peak concentrations of 1-3 days due to high hydraulic residence time, where calculations from instantaneous measurements produced erroneous estimates of performance and misleading relationships between residence time and removal. Internal porewater sampling wells showed differences in NO 3 concentration between shallow and deep zones, and "hot spot" zones where peak NO 3 removal co-occurred with dissolved oxygen depletion and dissolved organic carbon production. Tracking NO 3 movement through the profile showed preferential flow occurring with slower flow in deeper woodchips, and slower flow further from the most direct flowpath from inlet to outlet. High-frequency, in situ data on inlet and outlet time series and internal porewater solute profiles of this initial work highlight several key areas for future research. (Published by Elsevier Ltd.)
Anais da Academia Brasileira de Ciencias [An Acad Bras Cienc] 2020 Oct 12; Vol. 92 (suppl 2), pp. e20180856. Date of Electronic Publication: 2020 Oct 12 (Print Publication: 2020).
Cellulose, Fermentation, Aspergillus, Bioreactors, and Lipase
We evaluated the conditions to produce lipase in solid-state cultivation using a recently isolated strain of Aspergillus brasiliensis 157f in bioreactors of different configurations: static flat-bed, plugged-flow bed with forced water-saturated aeration, and pilot-scale rotating drum bioreactor, using malt bagasse as substrate. Lipase production was optimized applying experimental design analysis, which showed optima parameters defined as pH 7.7, addition of 11.3 % of soybean oil to the medium, and culture temperature of 32.7 oC, in static flat-bed. The highest enzyme activity (9.8 U.g-1 substrate) was obtained in the plugged-flow bed with forced water-saturated aeration. The fermented culture medium was lyophilized to create a solid enzymatic preparation (SEP), which was used to test the possibility of using this cheap biocatalyst in bioreactors to mediate esterification and transesterification reactions. SEP presented lipase activities of 7.35 U.g-1 substrate, indicating the possibility of further enhancing aspects of the use of such biocatalyst.
3D printing is a rapidly evolving field for biological (bioprinting) and non-biological applications. Due to a high degree of freedom for geometrical parameters in 3D printing, prototype printing of bioreactors is a promising approach in the field of Tissue Engineering. The variety of printers, materials, printing parameters and device settings is difficult to overview both for beginners as well as for most professionals. In order to address this problem, we designed a guidance including test bodies to elucidate the real printing performance for a given printer system. Therefore, performance parameters such as accuracy or mechanical stability of the test bodies are systematically analysed. Moreover, post processing steps such as sterilisation or cleaning are considered in the test procedure. The guidance presented here is also applicable to optimise the printer settings for a given printer device. As proof of concept, we compared fused filament fabrication, stereolithography and selective laser sintering as the three most used printing methods. We determined fused filament fabrication printing as the most economical solution, while stereolithography is most accurate and features the highest surface quality. Finally, we tested the applicability of our guidance by identifying a printer solution to manufacture a complex bioreactor for a perfused tissue construct. Due to its design, the manufacture via subtractive mechanical methods would be 21-fold more expensive than additive manufacturing and therefore, would result in three times the number of parts to be assembled subsequently. Using this bioreactor we showed a successful 14-day-culture of a biofabricated collagen-based tissue construct containing human dermal fibroblasts as the stromal part and a perfusable central channel with human microvascular endothelial cells. Our study indicates how the full potential of biofabrication can be exploited, as most printed tissues exhibit individual shapes and require storage under physiological conditions, after the bioprinting process.