Al Youha, Sarah, Alowaish, Osama, Ibrahim, Islam K., Alghounaim, Mohammad, Abu-Sheasha, Ghada A., Fakhra, Zainab, Al Hendi, Sarah, AlQabandi, Yousif, Almazeedi, Sulaiman, Al Asoomi, Fatima, and Al-Sabah, Salman
Journal of infection and public health. 14(9):1226-1232
Alfraij, Abdulla, Bin Alamir, Abdulrahman A., Al-Otaibi, Abdulnasir M., Alsharrah, Danah, Aldaithan, Abdulrahman, Kamel, Ahmed M., Almutairi, Muna, Alshammari, Salman, Almazyad, Mohammed, Macarambon, Jara Mia, and Alghounaim, Mohammad
Journal of infection and public health. 14(2):193-200
Infection Control & Hospital Epidemiology; September 2016, Vol. 37 Issue: 9 p1087-1093, 7p
BACKGROUNDPolymerase chain reaction (PCR) assays based on the detection of the toxin B gene are replacing enzyme-linked immunosorbent assay (ELISA)–based toxin production detection or cell cytotoxicity assay in most laboratories.OBJECTIVETo determine the proportion of pediatric patients diagnosed withClostridium difficileinfection by PCR who would have also been diagnosed by ELISA and to compare the clinical characteristics of PCR+/ELISA+ vs PCR+/ELISA− patients.METHODSUsing the microbiology laboratory information system, stool samples positive for C. difficileby PCR between October 2010 and July 2014 were identified. Using frozen stool specimens, an ELISA for toxin A and B was performed. A retrospective medical chart review was conducted to obtain demographic and clinical data. Duplicate samples were excluded.RESULTSA total of 136 PCR-positive samples underwent ELISA testing: 54 (40%) were positive for toxin A or B. The mean (SD) age of the entire cohort was 8.5 (6.2) years. There was no difference in age, gender, clinical manifestation, previous medical problems, and management between patients positive or negative by ELISA. However, patients positive by ELISA were more likely to have had a recent exposure to antibiotics (67.9% vs 50%; crude odds ratio, 2.1 [95% CI, 1.03–4.28]).CONCLUSIONIn our pediatric population, 60% of patients with C. difficilediagnosed by PCR had no toxin detectable by ELISA. ELISA-negative patients were less likely to have received an antibiotic recently compared with ELISA-positive patients. These results highlight the need to standardize laboratory criteria for the diagnosis of C. difficileinfections in children.Infect Control Hosp Epidemiol2016;37:1087–1093
Journal of Clinical Microbiology; August 2020, Vol. 58 Issue: 11
Case identification, isolation, and contact tracing are fundamental strategies used to control the spread of coronavirus disease 2019 (COVID-19). This has led to widespread testing that interrupted the supply chain for testing materials around the world. A prospective study was conducted to compare inexpensive and easily sourced 3-dimensionally (3D)-printed polylactic acid and polyester nasopharyngeal swabs to commercially manufactured swabs for the detection of severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2).