Chemistry & Biodiversity. Dec 2019, Vol. 16 Issue 12, n/a
Bleomycin -- Intellectual property, Bone morphogenetic proteins, Stem cells, Lung diseases, Obstructive, Medical research, Medicine, Experimental, Transforming growth factors, and Pulmonary fibrosis
Keywords: Qing-Xuan granule; pulmonary fibrosis; epithelial-mesenchymal transition (EMT); TGF-[beta]/Smad Abstract Pulmonary fibrosis (PF) is a chronic obstructive pulmonary disease without effective clinical drug treatment. Qing-Xuan Granule (QX) as a traditional Chinese patent medicine is clinically used to cure children's cough. This study was designed to investigate the effects of QX and possible molecular mechanisms for bleomycin-induced PF. The work used Western blotting and Q-PCR to explore the vitro and vivo mechanisms of QX treatment, while using HPLC-TOF/MS to explore the composition of QX. QX was given daily orally for two weeks after bleomycin intratracheal instillation. The protective effects of QX on lung function, inflammation, growth factors, hydroxyproline content and deposition of extracellular matrix were investigated. QX decreased expression of Col I and [alpha]-SMA in lung tissues by down-regulating TGF-[beta]1-Smad2/3 signaling and suppressed epithelial-mesenchymal transition and effectively reversed abnormal mRNA levels of MMP-1and TIMP-1 as well as LOXL-2 in lung tissues. HPLC-TOF/MS indicate that six substances could be the main active components, which were reported to protect against experimental lung disease. Article Note: These authors contributed equally to this work. Byline: , , Haitao Ge, Haitao Tang,Renshi Li, Chaofeng Zhang
Xu, Peng, Lyu, Lulu, Sami, Muhammad Umair, Lu, Xin, Ge, Haitao, Rong, Yutao, Hu, Chunfeng, and Xu, Kai
Experimental and Therapeutic Medicine. Dec 2018, Vol. 16 Issue 6, p4873, 6 p.
Hepatic vein thrombosis -- Diagnosis, Hepatic vein thrombosis -- Care and treatment, Angiography -- Usage, and Magnetic resonance imaging -- Usage
Introduction Budd-Chiari syndrome (BCS) is characterized by the blockage of the hepatic veins (HVs) and/or the inferior vena cava (IVC) (1). Myeloproliferative disorders are the most common causes of BCS [...] In recent years, the role of magnetic resonance angiography (MRA) in the diagnosis of Budd-Chiari Syndrome (BCS) has been the focus of various clinical studies. The purpose of the present study was to perform a meta-analysis of the diagnostic performance of MRA in patients with BCS by using digital subtraction angiography as a reference method. The search strategy for relevant research articles was based on the Cochrane Handbook for Systematic Reviews, and literature databases (including PubMed, Medline and China National Knowledge Infrastructure) and reference lists of retrieved studies published from 2000 to 2016 were searched. The Quality Assessment of Diagnostic Accuracy Studies tool was used to assess the methodological quality of these research studies by two reviewers independently. Summary estimates of the sensitivity, specificity, positive/negative likelihood ratio (LR+/-), diagnostic odds ratio (DOR) and the summary receiver operating characteristic (SROC) curve of MRA in identifying BCS were obtained. The pooled MRA estimates had a sensitivity of 97.6% [95% confidence interval (CI), 95.1-99.0%], a specificity of 70.7% (95% CI, 54.5-83.9%), an LR+ of 3.163 (95% CI, 2.03-4.94) and an LR- of 0.045 (95% CI, 0.02-0.09). The overall DOR was 94.053 (95% CI, 32.71-270.41). The area under the SROC curve was 0.972. In conclusion, MRA is an accurate modality for evaluating BCS. Key words: magnetic resonance angiography, Budd-Chiari syndrome, meta-analysis, hepatic vein, diagnostic accuracy
Byline: Haitao Ge, Longfa Fang, Xiahe Huang, Jinlong Wang, Weiyang Chen, Yuanya Zhang, Xiaorong Wang, Na Sui, Wu Xu, Qingfang He, Yingchun Wang Keywords: carbon metabolism; Hik33; photosynthesis; stress response Abstract The histidine kinase Hik33 plays a central role in acclimation to changing environments in cyanobacteria. Deletion of hik33 induces a strong stress-like response in Synechocystis sp. PCC 6803 (Synechocystis) as represented by repressed photoautotrophic growth and photosynthesis, and differential expression of stress-responsive proteins. In contrast, the photomixotrophic growth of the hik33-deletion mutant (Ihik33) with glucose as the exogenous carbon source is only marginally repressed. To investigate how glucose rescues the growth of Ihik33, the proteomes of the photomixotrophically growing wild-type (WT) and the mutant strains of Synechocystis are quantitatively analyzed. It is found that glucose induces upregulation of the oxidative pentose phosphate (OPP) pathway. Depletion of glucose-6-phosphate dehydrogenase (G6PDH), which catalyzes the first and the rate-limiting step of the OPP pathway, significantly inhibits the photomixotrophic growth of Ihik33 but not of the WT. The result suggests that the OPP pathway, which is usually nonfunctional in the photomixotrophically growing WT, plays a major role in the photomixotrophic growth of Ihik33. CAPTION(S): Supporting information.
International Journal of Cancer. Oct 1, 2017, Vol. 141 Issue 7, p1434, 11 p.
T cells, Macrophages, Brain tumors, and Gene expression
Byline: Haitao Ge, Luyan Mu, Linchun Jin, Changlin Yang, Yifan (Emily) Chang, Yu Long, Gabriel DeLeon, Loic Deleyrolle, Duane A. Mitchell, Paul S. Kubilis, Dunyue Lu, Jiping Qi, Yunhe Gu, Zhiguo Lin,Jianping Huang Tumor migration/metastasis and immunosuppression are major obstacles in effective cancer therapy. Incidentally, these 2 hurdles usually coexist inside tumors, therefore making therapy significantly more complicated, as both oncogenic mechanisms must be addressed for successful therapeutic intervention. Our recent report highlights that the tumor expression of a TNF family member, CD70, is correlated with poor survival for primary gliomas. In this study, we investigated how CD70 expression by GBM affects the characteristics of tumor cells and the tumor microenvironment. We found that the ablation of CD70 in primary GBM decreased CD44 and SOX2 gene expression, and inhibited tumor migration, growth and the ability to attract monocyte-derived M2 macrophages in vitro. In the tumor microenvironment, CD70 was associated with immune cell infiltrates, such as T cells; myeloid-derived suppressor cells; and monocytes/macrophages based on the RNA-sequencing profile. The CD163+ macrophages were far more abundant than T cells were. This overwhelming level of macrophages was identified only in GBM and not in low-grade gliomas and normal brain specimens, implying their tumor association. CD70 was detected only on tumor cells, not on macrophages, and was highly correlated with CD163 gene expression in primary GBM. Additionally, the co-expression of the CD70 and CD163 genes was found to correlate with decreased survival for patients with primary GBM. Together, these data suggest that CD70 expression is involved in promoting tumor aggressiveness and immunosuppression via tumor-associated macrophage recruitment/activation. Our current efforts to target this molecule using chimeric antigen receptor T cells hold great potential for treating patients with GBM.
Gene expression -- Health aspects, Ligases -- Health aspects, Glioblastoma multiforme -- Genetic aspects, Glioblastoma multiforme -- Development and progression, Glioblastoma multiforme -- Care and treatment, and Transcription factors -- Health aspects
Abstract. Glioblastoma multiforme (GBM), one of the most aggressive human malignant brain tumors, is induced by multiple complex pathological mechanisms. The main cause of mortality in patients with GBM is [...]