Nash MA, Hoffman JM, Stevens DY, Hoffman AS, Stayton PS, and Yager P
Lab On A Chip [Lab Chip] 2010 Sep 07; Vol. 10 (17), pp. 2279-82. Date of Electronic Publication: 2010 Jul 06.
Animals, Antibodies, Immobilized chemistry, Antibodies, Immobilized immunology, Biosensing Techniques economics, Clinical Laboratory Techniques, Equipment Design, Gold chemistry, Humans, Immunoassay economics, Immunoglobulin G chemistry, Immunoglobulin G immunology, Metal Nanoparticles chemistry, Proteins immunology, Streptavidin chemistry, Streptavidin immunology, Syringes, Time Factors, Biosensing Techniques instrumentation, Immunoassay instrumentation, Paper, Proteins chemistry, and Reagent Strips
A method for patterning narrow lines of biomolecules onto nitrocellulose membranes using laboratory syringe pumps is described. One syringe pump is used to drive the biomolecule solution through a needle, while another modified syringe pump acts as a one-dimensional translation stage, moving the needle across the membrane much like a pen. This method consumes very small volumes of reagents, and is a viable option for laboratory-scale fabrication and prototyping of point-of-care rapid diagnostic test strips.