Sommer, Ulf, Herscovitz, Haya, Welty, Francine K., and Costello, Catherine E.
Journal of lipid research, 2006 Apr., v. 47, no. 4, p. 804-814.
Includes references A simple and robust LC-MS-based methodology for the investigation of lipid mixtures is described, and its application to the analysis of human lipoprotein-associated lipids is demonstrated. After an optional initial fractionation on Silica 60, normal-phase HPLC-MS on a YMC PVA-Sil column is used first for class separation, followed by reversed-phase LC-MS or LC-tandem mass spectrometry using an Atlantis dC18 capillary column, and/or nanospray MS, to fully characterize the individual lipids. The methodology is applied here for the analysis of human apolipoprotein B-associated lipids. This approach allows for the determination of even low percentages of lipids of each molecular species and showed clear differences between lipids associated with apolipoprotein B-100-LDL isolated from a normal individual and those associated with a truncated version, apolipoprotein B-67-containing lipoproteins, isolated from a homozygote patient with familial hypobetalipoproteinemia. The methods described should be easily adaptable to most modern MS instrumentation.
Millar, John S., Brousseau, Margaret E., Diffenderfer, Margaret R., Barrett, P. Hugh R., Welty, Francine K., Cohn, Jeffrey S., Wilson, Aisha, Wolfe, Megan L., Nartsupha, Chorthip, Schaefer, Peter M., Digenio, Andres G., Mancuso, James P., Dolnikowski, Gregory G., Schaefer, Ernst J., and Rader, Daniel J.
In Journal of Lipid Research March 2008 49(3):543-549