Pediatric And Developmental Pathology: The Official Journal Of The Society For Pediatric Pathology And The Paediatric Pathology Society [Pediatr Dev Pathol] 2015 Nov-Dec; Vol. 18 (6), pp. 477-80. Date of Electronic Publication: 2015 Dec 23.
Adolescent, Age Factors, Anatomy education, Child, Child, Preschool, Education, Medical history, Female, History, 19th Century, History, 20th Century, Humans, Infant, Infant, Newborn, Male, Pediatrics education, Anatomy history, Medical Illustration history, Pediatrics history, and Reference Books, Medical
Current Opinion In Pediatrics [Curr Opin Pediatr] 2005 Dec; Vol. 17 (6), pp. 809-18.
Athletic Injuries prevention control, Child, Child Health Services standards, Health Services Accessibility economics, Health Services Accessibility standards, Humans, Insurance, Health trends, Sleep Wake Disorders diagnosis, Sleep Wake Disorders drug therapy, United States, Child Health Services economics, and Insurance, Health economics
Deist TM, Dankers FJWM, Valdes G, Wijsman R, Hsu IC, Oberije C, Lustberg T, van Soest J, Hoebers F, Jochems A, El Naqa I, Wee L, Morin O, Raleigh DR, Bots W, Kaanders JH, Belderbos J, Kwint M, Solberg T, Monshouwer R, Bussink J, Dekker A, and Lambin P
Medical Physics [Med Phys] 2018 Jul; Vol. 45 (7), pp. 3449-3459. Date of Electronic Publication: 2018 Jun 13.
Area Under Curve, Chemoradiotherapy adverse effects, Decision Trees, Humans, Logistic Models, Neoplasms mortality, Neural Networks (Computer), Prognosis, Software, Chemoradiotherapy methods, Machine Learning, Neoplasms diagnosis, and Neoplasms radiotherapy
Biochemistry [Biochemistry] 2018 Apr 03; Vol. 57 (13), pp. 1929-1938. Date of Electronic Publication: 2018 Mar 23.
Animals, Humans, Molecular Imaging methods, and Staining and Labeling methods
Qualitative imaging of biomolecular localization and distribution inside cells has revolutionized cell biology. Most of these powerful techniques require modifications to the target biomolecule. Over the past 10 years, these techniques have been extended to quantitative measurements, from in-cell protein folding rates to complex dissociation constants to RNA lifetimes. Such measurements can be affected even when a target molecule is just mildly perturbed by its labels. Here, the impact of labeling on protein (and RNA) structure, stability, and function in cells is discussed via practical examples from the recent literature. General guidelines for selecting and validating modification sites are provided to bring the best from cell biology and imaging to quantitative biophysical experiments inside cells.
Chromatin spread techniques have been widely used to assess the dynamic localization of various proteins during gametogenesis, particularly for spermatogenesis. These techniques allow for visualization of protein and DNA localization patterns during meiotic events such as homologous chromosome pairing, synapsis and DNA repair. While a few protocols have been described in the literature, general chromatin spread techniques using mammalian prophase oocytes are limited and difficult due to the timing of meiosis initiation in fetal ovaries. In comparison, prophase spermatocytes can be collected from juvenile male mice with higher yields without the need for microdissection. However, it is difficult to obtain a pure synchronized population of cells at specific stages due to the heterogeneity of meiotic and post-meiotic germ cell populations in the juvenile and adult testis. For later stages of meiosis, it is advantageous to assess oocytes undergoing meiosis I (MI) or meiosis II (MII), because groups of mature oocytes can be collected from adult female mice and stimulated to resume meiosis in culture. Here, methods for meiotic chromatin spread preparations using oocytes dissected from fetal, neonatal and adult ovaries are described with accompanying video demonstrations. Chromosome missegregation events in mammalian oocytes are frequent, particularly during MI. These techniques can be used to assess and characterize the effects of different mutations or environmental exposures during various stages of oogenesis. As there are distinct differences between oogenesis and spermatogenesis, the techniques described within are invaluable to increase our understanding of mammalian oogenesis and the sexually dimorphic features of chromosome and protein dynamics during meiosis.
Organic & Biomolecular Chemistry [Org Biomol Chem] 2016 Jun 21; Vol. 14 (23), pp. 5251-7. Date of Electronic Publication: 2016 May 17.
Catalysis, Models, Molecular, Molecular Conformation, Quantum Theory, Anilides chemistry, Carbon chemistry, Hydrogen chemistry, Palladium chemistry, Urea chemistry, and Water chemistry
C-H activation plays a central role in organometallic catalysis. Concerted metallation-deprotonation (CMD) has been dominant as the pathway for C-H bond cleavage. In the course of studying the mechanism of C-H activation of arylamides and arylureas with Pd complexes as part of catalytic oxidative Heck reactions, DFT calculations were carried out. The turnover-limiting C-H activation is acid-catalysed and can occur readily in the absence of acetate or other coordinating bases. The calculations simulated experiment, so that ligated sulfonate and water, both previously observed by X-ray characterization, were incorporated in the model. A Wheland-type complex between acetanilide and Pd was readily located, but the reactive C-H and the coordinated sulfonate were poorly placed for intramolecular proton transfer. Involvement of a water molecule coordinated to sulfonate provides a low-energy pathway to the palladacycle. The relative reactivity of substituted acetanilides and arylureas according to this model fits well with existing literature. General-base catalysis as described here has broader potential.