lab-on-a-chip, bioassay, toxicity, additive manufacturing, polymers, 3D printing, Mechanical engineering and machinery, and TJ1-1570
Additive manufacturing (AM) is ideal for building adaptable, structurally complex, three-dimensional, monolithic lab-on-chip (LOC) devices from only a computer design file. Consequently, it has potential to advance micro- to milllifluidic LOC design, prototyping, and production and further its application in areas of biomedical and biological research. However, its application in these areas has been hampered due to material biocompatibility concerns. In this review, we summarise commonly used AM techniques: vat polymerisation and material jetting. We discuss factors influencing material biocompatibility as well as methods to mitigate material toxicity and thus promote its application in these research fields.
Materials Science, Mechanical engineering, Biomedical engineering, 3D Printing, Bioassay, ELISA, Fused Deposition Modeling, Manufacturing, and Stereolithography
Fabrication of a microfluidic ELISA assay can be a very time-consuming method, due to the curing process required for molded parts. This thesis examines Fused Deposition Modeling and Stereolithography as candidates for rapid prototyping microfluidic devices. Individual components of the device were designed on SolidWorks, and underwent several generations of revisions to address problems of air and fluid leakage. We present an automated ELISA assay device created using a combination of Fused Deposition Modeling and Stereolithography as a comprehensive demonstration of additive manufacturing capabilities, as well as the methodology used to create such a device. A detailed explanation on how to troubleshoot the fabrication process and machines is also discussed.