Schuller-Ravoo, Sigrid, Zant, Erwin, Feijen, Jan, and Grijpma, Dirk W.
Advanced Healthcare Materials. Dec 2014, Vol. 3 Issue 12, p2004, 8 p.
Tissue engineering, Carbonates, and Rapid prototyping
Byline: Sigrid Schuller-Ravoo, Erwin Zant, Jan Feijen, Dirk W. Grijpma Keywords: photocross-linking; poly(trimethylene carbonate); rapid prototyping; stereolithography; microvascular networks; tissue-engineering scaffolds Designed flexible and elastic network structures are prepared by stereolithoA-graphy using a photo-crosslinkable resin based on a poly(trimethylene carbonate) (PTMC) macromer with a molecular weight of 3150 g/mol. Physical properties and the compatibility with human umbilical vein endothelial cells (HUVECs) are evaluated. The hydrophobic networks are found to be flexible and elastic, with an E modulus of 7.9 [+ or -] 0.1 MPa, a tensile strength of 3.5 [+ or -] 0.1 MPa and an elongation at break of 76.7 [+ or -] 0.7%. HUVECs attach and proliferate well on the surfaces of the built structures. A three-dimensional microvascular network is designed to serve as a perfusable scaffold for tissue engineering. In the design, 5 generations of open channels each branch into 4 smaller channels yielding a microvascular region with a high density of capillaries. The overall cross-A-sectional area through which medium or blood can be perfused remains constant. These structures would ensure efficient nourishment of cells in a large volume of tissue. Built by stereolithography using the PTMC resin, the smallest channels of these structures have square cross-sectional areas, with inner widths of approximately 224 I1/4m and wall thicknesses of approximately 152 I1/4m. The channels are open, allowing water to perfuse the scaffold at 0.279 [+ or -] 0.006 mL/s at 80 mmHg and 0.335 [+ or -] 0.009 mL/s at 120 mmHg. Supporting information: Additional Supporting Information may be found in the online version of this article As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. CAPTION(S): Supplementary