Goncalves, Ana I., Rodrigues, Marcia T., Carvalho, Pedro P., Banobre-Lopez, Manuel, Paz, Elvira, Freitas, Paulo, and Gomes, Manuela E.
Advanced Healthcare Materials. Jan 2016, Vol. 5 Issue 2, p213, 10 p.
Tissue engineering -- Magnetic properties, Tissue engineering -- Analysis, Collagen -- Magnetic properties, Collagen -- Analysis, Ferric oxide -- Magnetic properties, Ferric oxide -- Analysis, Rapid prototyping -- Magnetic properties, Rapid prototyping -- Analysis, Magnetic fields -- Magnetic properties, Magnetic fields -- Analysis, Stem cells -- Magnetic properties, and Stem cells -- Analysis
Byline: Ana I. Goncalves, Marcia T. Rodrigues, Pedro P. Carvalho, Manuel Banobre-Lopez, Elvira Paz, Paulo Freitas, Manuela E. Gomes Keywords: adipose stem cells; magnetic nanoparticles; magnetic scaffolds; tendons; tissue engineering The application of magnetic nanoparticles (MNPs) in tissue engineering (TE) approaches opens several new research possibilities in this field, enabling a new generation of multifunctional constructs for tissue regeneration. This study describes the development of sophisticated magnetic polymer scaffolds with aligned structural features aimed at applications in tendon tissue engineering (TTE). Tissue engineering magnetic scaffolds are prepared by incorporating iron oxide MNPs into a 3D structure of aligned SPCL (starch and polycaprolactone) fibers fabricated by rapid prototyping (RP) technology. The 3D architecture, composition, and magnetic properties are characterized. Furthermore, the effect of an externally applied magnetic field is investigated on the tenogenic differentiation of adipose stem cells (ASCs) cultured onto the developed magnetic scaffolds, demonstrating that ASCs undergo tenogenic differentiation synthesizing a Tenascin C and Collagen type I rich matrix under magneto-stimulation conditions. Finally, the developed magnetic scaffolds were implanted in an ectopic rat model, evidencing good biocompatibility and integration within the surrounding tissues. Together, these results suggest that the effect of the magnetic aligned scaffolds structure combined with magnetic stimulation has a significant potential to impact the field of tendon tissue engineering toward the development of more efficient regeneration therapies. Supporting information: Additional Supporting Information may be found in the online version of this article As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. CAPTION(S): Supplementary