Al-Qallaf, Danah A. A. A. Q., Tomlinson, Darren, Bell, Sandra, and McPherson, Michael
Biomarkers are molecules present in the patients' samples. They are used to detect the presence of a disease or an infection. An array of laboratory tools is used to monitor changes in the biomarker levels for diagnostic and prognostic purposes. Affimers are relatively new tools, which can be used in similar ways to commonly used antibodies with many advantages over the later. The work described in this PhD thesis has focused on a number of methodologies for the generation of Affimers against purified proteins and cell-surface molecules, with the intention of using these to detect biomarkers in cancer. To ensure the development of functional Affimer reagents against different membrane target molecules, EGFR, HER2, and HER3 were used as models to optimise the process of Affimers isolation and selection using phage display technology. In vitro production of Affimers using bacterial cells was assessed and optimised. Following optimisation, a range of Affimers that bound to EGFR, HER2, and HER3 were generated and partially characterised by different molecular applications, including immunofluorescence, immunohistochemistry, and pull-down assay. Upon characterisation, the developed reagents were not only able to identify their targets on cells and to precipitate them down from cell lysates, but also exhibited a complete inhibition of the downstream signalling activation of both EGFR and HER3. In addition, this thesis provides clear evidence of the potential of Affimers in biomarker discovery studies. After multiple rounds on non-tumourigenic (HB2) and cancerous (MDA-MB-453) breast-cell lines, eight novel anti-breast-cancer Affimers were successfully isolated and characterised. Following pull-down assay and mass spectrometry analysis, the target proteins to which these Affimers were found to bind was identified as CK18 and 19. Upon identification, the specificity of Affimers was further validated on a panel of breast cells, tissues, and on multiple breast-tissue microarrays (TMAs). There is a need to develop the knowledge to utilise this new Affimers-based technology to encourage adoption of this useful tool. With the aid of such technology, several novel detection reagents were generated, partially validated and proved to be promising tools for biomarker detection in different conventional assays.
Childhood obesity has reached epidemic proportions globally, and poses a considerable burden on a child’s short- and long-term health. Most obesity presents in the early (preschool) years, and once established tracks into later life. Therefore, identification of risk factors in the preschool period is considered critical for prevention of long-term obesity. In the United Arab Emirate (UAE), the rising prevalence of childhood obesity is of great public health concern. However, no previous study has explored risk factors for obesity in preschool children. This PhD aimed to: (i) identify risk factors for preschool obesity in the UAE (Study 1); (ii) describe dietary intake and patterns of preschool children, and explore their associations with risk of obesity (Study 2); and (iii) in a randomised controlled trial investigate the effectiveness of the ‘Eat Right Emirates’ (ERE) tool, a simple leaflet intervention designed to encourage a healthy lifestyle and prevent preschool obesity (Study 3). Study 1 showed that a longer duration of breastfeeding, and later introduction of complementary foods were associated with lower BMI z-score. Study 2 found that, compared with UK dietary guidelines, preschool children in the UAE exceeded intakes of protein, but did not meet recommended intakes for fibre. A high carbohydrate intake as a percentage of energy was associated with lower BMI z-score, whereas a high fat intake was associated with higher BMI z-score. A priori derived diet score found diets of preschool children were suboptimal, and principal component analysis identified three dietary patterns (‘traditional/health-conscious’, ‘processed/western’ and ‘convenience/snack’), which were not associated with BMI z-score. Study 3 found that the ERE tool was effective in reducing obesity risk compared to controls at 6-month follow-up. These findings, and the high compliance rate, suggest that the simple intervention is a promising approach for prevention of obesity in the UAE.
This research considers the possibility of a locally-centric design education curricula in Amman, Jordan by investigating the philosophies, theories, practices and models of curriculum and pedagogy most appropriate for design education. It describes perceptions of design and examines the possibilities for shifting these perceptions to move towards transforming design education. Jordan is a neopatriarchal society, and education re-enacts the dominant structures of the state within curriculum and pedagogy centred on the authority of the educator. This thesis argues for a decolonised design education based on a student-centred pedagogy drawn from the process and praxis curriculum models - a design education and design otherwise. Working with a range of designers, students and educators, it investigates the potential of these actors to contribute to the development of a pedagogy for design education in Jordan that is relevant to the milieu and locality. It poses the following questions: What philosophies, theories, practices, models of curriculum, and pedagogy are appropriate?; What potential shifts could this require and create?; How do we shift perceptions? This qualitative research uses interviews, focus groups, and design charrettes for data collection. Through participation and engagement with people that have most at stake in design education - designers, design educators and design students - I argue for an emancipatory design education that reflects on design beyond its traditional service-provider definition. Drawing on scholarship from design and education studies, and literature from fields such as history, decolonial studies, architecture and urbanism, political science, economics and philosophy, I argue for a curriculum model and student-centred pedagogy that considers design's role in society. Literature on Arab higher education is preoccupied with reforms to help the Arab region build a knowledge-society without considering the role of curriculum models and pedagogy nor addressing power structures. In addition, within design, little literature exists on the Arab region or Jordan, leaving its design culture(s) largely undocumented. My thesis investigates design education in higher education in Jordan by concentrating on models of pedagogy and curriculum and provides an overview of Jordan's contemporary design culture.
Mishra, Punya, Henriksen, Danah, and Deep-Play Research Group
Cognitive learning and Educational technology
In this collection of beautifully written essays, Mishra, Henriksen, and the Deep-play Research Group challenge myths about technology and creativity, debate time-honored instructional practices, and play with new ideas for schools to care for and nurture, rather than constrain, creativity. These essays are provocative … refreshing, [and] insightful —Dr. Yong Zhao, Foundation Distinguished Professor, University of Kansas and Fellow, Mitchell Institute for Health and Education Policy, Victoria University, Australia. What is creativity? Why is it important? What does it look like across different disciplines and contexts? What role does technology play, if any, in the creative process? And finally, what do creativity and technology have to do with education? These are the questions that underlie the collection of articles in this book. These essays provide a broad analytic frame for thinking about creativity, technology and education and describe classroom examples as well as strategies for evaluating creative artifacts and creative environments. All of these are grounded in specific examples from across a wide range of disciplines and contexts—art, mathematics, engineering, computer science, graphic design, architecture, science to name just a few. The final essays take a broader perspective on creativity and technology focusing both on our highly inter-connected YouTube world but also possibilities for the future. Creativity, Technology & Education: Exploring their Convergence is a vital resource for educators and practitioners as they seek to incorporate creative work and thoughtful pedagogy in their personal and professional lives.
This book focuses on rethinking creativity for 21st century education. The specific emphasis examines the way that creativity spans disciplines, through a set of common thinking skills that the most accomplished thinkers in any field use. These seven transdisciplinary thinking skills are rooted in historical exemplars of creativity across disciplines. We examine these skills in more detail, chapter by chapter, to offer examples of what each skill looks like in disciplines ranging from art to science, or music to math, and beyond. This set of thinking skills reflects the way that creativity may look different across fields, yet there are common paths of creative thinking that cut across disciplinary boundaries. Beyond this each chapter also considers applications for such skills in 21st century educational contexts, with an eye toward creative teaching and technology. In all of this, the book weaves together broad cultural examples of creativity and the seven transdisciplinary skills, alongside specific application-based examples from technology and teacher education.
Technology and youth, Youth--Social life and customs, Internet and youth, Online social networks, and Youth--Political activity
In the last two decades, both the conception and the practice of participatory culture have been transformed by the new affordances enabled by digital, networked, and mobile technologies. This exciting new book explores that transformation by bringing together three leading figures in conversation. Jenkins, Ito and boyd examine the ways in which our personal and professional lives are shaped by experiences interacting with and around emerging media.Stressing the social and cultural contexts of participation, the authors describe the process of diversification and mainstreaming that has transformed participatory culture. They advocate a move beyond individualized personal expression and argue for an ethos of “doing it together” in addition to “doing it yourself.”Participatory Culture in a Networked Era will interest students and scholars of digital media and their impact on society and will engage readers in a broader dialogue and conversation about their own participatory practices in this digital age.
Quantum theory, Organizational change, Leadership, and Physics--Philosophy
Drawing inspiration from quantum physics, innovative management thinker Danah Zohar offers a powerful new model for business thinking and practice.'Quantum leaders,'she says, like the systems they have to manage, are poised at'the edge of chaos.'They thrive on the potential latent in uncertainty and are adept at unleashing the creativity of self-organization. More important, they are vision- and value-led; they adapt quickly, are unafraid to play with the boundaries and reinvent the rules, and celebrate diversity.Zohar points out that the existing, business-as-usual paradigm owes a great deal to the outdated thinking, assumptions, and values of Newtonian science, which gave rise to the Industrial Revolution. Newtonian thinking assumes that corporations and markets are like machines--predictable, stable, and controllable; they are best managed in a way that eliminates risk and assures equilibrium. Unfortunately, as the global financial collapse of 2008 demonstrated, this way of thinking is as obsolete as the steam engine.Further developing ideas she introduced in her acclaimed Rewiring the Corporate Brain and Spiritual Capital, Zohar has written an inspirational book that will motivate leaders to tap the full potential of their employees, their businesses, and the customers they serve.
Che succede quando gli adolescenti comunicano mediante i social network come Facebook, Twitter e Instagram? Come gestiscono la propria identità, come difendono la propria privacy, che cosa mettono in gioco dei propri sentimenti? Queste sono solo alcune delle domande che danah boyd, autrice di successo ed esperta di social media e comunicazione, affronta nel suo libro dedicato a quella second life che rappresenta ormai la forma di vita più importante per la maggioranza degli adolescenti. L'autrice sfata i falsi miti legati alla diffusione di internet, dal presunto aumento del cosiddetto cyberbullismo agli esagerati pericoli sulla spersonalizzazione dei giovani, fino alla stessa definizione di nativi digitali, troppo semplicistica rispetto alla complessità del fenomeno. It's complicated, frutto di dieci anni di ricerca sul campo, di interviste e di incontri con centinaia di adolescenti, è una lettura fondamentale per chiunque sia interessato a comprendere l'impatto sociale e culturale delle nuove tecnologie di comunicazione sulle generazioni future.
Customer services, Customer relations, and Consumer satisfaction
The success of service organizations ultimately depends on employees in customer service roles; their performance affects customer satisfaction and loyalty. Integrating theory and research, this book explores the factors that determine the performance of service providers, and points to important managerial implications.