Indian Journal of Orthopaedics. July-August, 2013, Vol. 47 Issue 4, 388
Tissue engineering, Stem cells, Collagen, Phosphate minerals, Phosphate rock, and Rapid prototyping
Background: In bone tissue engineering, extracellular matrix exerts critical influence on cellular interaction with porous biomaterial and the apatite playing an important role in the bonding process of biomaterial to [...]
BioMed Research International. March 2020, Vol. 2019
Construction equipment industry -- International economic relations, Penicillin G, Stem cells, Ethylenediaminetetraacetic acid, Tissue engineering, and Rapid prototyping
1. Introduction Bone defects resulting from congenital deformity, trauma, inflammation, and tumor resection can cause patients great pain and impose a tremendous burden on health-care systems. Thus, bone substitutes are [...] Poly-[epsilon]-caprolactone (PCL) is a promising synthetic material in bone tissue engineering (BTE). Particularly, the introduction of rapid prototyping (RP) represents the possibility of manufacturing PCL scaffolds with customized appearances and structures. Bio-Oss is a natural bone mineral matrix with significant osteogenic effects; however, it has limitations in being constructed and maintained into specific shapes and sites. In this study, we used RP and fabricated a hollow-structured cage-shaped PCL scaffold loaded with Bio-Oss to form a hybrid scaffold for BTE. Moreover, we adopted NaOH surface treatment to improve PCL hydrophilicity and enhance cell adhesion. The results showed that the NaOH-treated hybrid scaffold could enhance the osteogenesis of human bone marrow-derived mesenchymal stem cells (hBMMSCs) both in vitro and in vivo. Altogether, we reveal a novel hybrid scaffold that not only possesses osteoinductive function to promote bone formation but can also be fabricated into specific forms. This scaffold design may have great application potential in bone tissue engineering.