Kang, Yun Gyeong, Shin, Ji Won, Park, So Hee, Kim, Young Mi, Gu, Seo Rin, Wu, Yanru, Ban, Hun Yeong, and Shin, Jung-Woog
Biotechnology letters, 2016 Jan., v. 38, no. 1, p. 175-181.
extracellular matrix, umbilical cord, stem cells, biomimetics, phenotype, and blood
OBJECTIVE: To investigate the expansion of hematopoietic stem/progenitor cells (HSPCs) from umbilical cord blood using extracellular matrix (ECM) protein-coated three-dimensional hierarchical scaffolds. RESULTS: The expansion of HSPCs was evaluated through total nucleated cell (TNC) expansion, immuno-phenotypic analysis, and clonogenic ability. After 7 days of culture, three-dimensional cultures with fibronectin-coated scaffolds achieved the highest fold increase in TNCs (164 ± 6.9 fold) and the highest CD45⁺CD34⁺ (35 %) and CD34⁺CD38⁻ (32 %) ratios. CONCLUSION: Three-dimensional hierarchical scaffolds were coated with ECM protein to simulate a biomimetic environment or niche, and had a significant effect on the expansion potential of HSPCs without changing their phenotype.