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chemical analysis, equipment design, mass spectrometry, and equipment performance

A fast and straightforward method to prototype microfluidic chip systems for dead-volume-free hyphenation to electrospray-ionisation mass spectrometry is presented. The developed approach based on liquid-phase lithography provides an inexpensive and reliable access to microfluidic chips for MS coupling which can be manufactured in any laboratory with low technical demands. The rapid prototyping approach enables the seamless integration of capillaries serving as electrospray emitters with negligible dead volume. The high versatility of the presented prototyping method and the applicability of a variety of chip-based devices in different fields of lab-on-a-chip technology are established for analytical separations by means of chip-electrochromatography–MS and for continuous-flow synthesis using microreactor technology with MS detection.

rapid prototyping, agriculture safety and health, job training program development, Education, PROGRAMMES, PROGRAMME, and PROGRAMAS

Summary(En)
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The purpose of this study was to develop a job training program that can deliver knowledge and skill necessary for agricultural safety and health manager. A new program development model applying rapid prototyping(RP) approach to the basic ISD model was established to develop the program. Then, a textbook for learners, a manual for instructors, additional learning material were produced based on the model. The major conclusions of this study were as follows. First, this study suggested a new type of program development model that combines RP model and linear ISD model that can apply opinion of participants to compensate the defect of traditional ISD model. Second, the material was developed by applying opinion of stakeholders of program development. By developing a prototype, interim finding, the users could evaluate it at first hand. Therefore, the materials contain the content knowledge, skill, tools(KST) that agriculture safety and health managers need when they perform the job. Also various types of activities were developed so that can the learners can apply the learned contents to the job. Third, the instructors' manual and supplementary material was developed based on learners' text book. The instructors' manual is a guidance that contains the details such as learning contents, procedure and methods so that standard lectures could be delivered regardless of who teaches. Also, supplementary material is the material contains intensified knowledge and skill related to the contents of learners' textbook, so that the instructors can check them with ease.

extracellular matrix, umbilical cord, stem cells, biomimetics, phenotype, and blood

OBJECTIVE: To investigate the expansion of hematopoietic stem/progenitor cells (HSPCs) from umbilical cord blood using extracellular matrix (ECM) protein-coated three-dimensional hierarchical scaffolds. RESULTS: The expansion of HSPCs was evaluated through total nucleated cell (TNC) expansion, immuno-phenotypic analysis, and clonogenic ability. After 7 days of culture, three-dimensional cultures with fibronectin-coated scaffolds achieved the highest fold increase in TNCs (164 ± 6.9 fold) and the highest CD45⁺CD34⁺ (35 %) and CD34⁺CD38⁻ (32 %) ratios. CONCLUSION: Three-dimensional hierarchical scaffolds were coated with ECM protein to simulate a biomimetic environment or niche, and had a significant effect on the expansion potential of HSPCs without changing their phenotype.

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TECHNOLOGY - Research and Development - Costs, COST ANALYSIS - Research and Development, and COST EFFECTIVENESS - Methodology

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