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Animal Science and Zoology and Ecology, Evolution, Behavior and Systematics

Dinidor jograziae sp. nov., is described. The new species can be recognized by the relatively shorter mandibular plates which do not come in contact with each other anterior to the clypeus, by the dark brown hemelytral membranes, with darker, reticulate veins, and by having a relatively large portion of the connexiva exposed. We provide high-resolution photographs of important characters such as the head, pronotum, and the male and female external genitalia. We also provide new locality records for D. saucius from southern and southeastern Brazil, including the Atlantic Rain Forest region in the states of Rio de Janeiro, Santa Catarina and São Paulo.

Biodiversity, Taxonomy, Animalia, Arthropoda, Insecta, Hemiptera, Dinidoridae, Dinidor, and Dinidor saucius

Dinidor saucius Stål 1870 Dinidor saucius Stål: 79; Lethierry and Severin, 1893: 235; Kirkaldy, 1909: 254; Schouteden, 1913: 5; Durai, 1987: 228, 231–232. 1873 Cyclopelta saucia: Walker: 27. 2015 Dinidor mactabilis: Schwertner and Grazia: 822, 827, 849, figs.; Genevcius et al., 2016: 1–4. Distribution. BRAZIL: Espírito Santo, Rio de Janeiro (Cachoeiras de Macacu [new rec.], Itatiaia [new rec.], and Rio de Janeiro); Santa Catarina (Joinville [new rec.]); São Paulo (Barueri [new rec.], Óleo [new rec.], Santos [new rec.], São Bernardo do Campo, São Paulo [new rec.]). Material examined. Brasil, SP, São Bernardo do Campo, Acampamento dos Engenheiros 23°44’52’’S, 46°38’13.71’’W, 11–12.Nov.2010, Bio de Campo UNIFESP (1♀); [Brasil] Barueri, São Paulo — Brasil, 28. III.1955, K. Lenko col. (1♀); Brasil, Joinville, S[an]ta. Catarina, Dirings col. [Agt 1956, in the back of the label] (2♀); Brasil, Joinville, Rio Bracinho, S[an]ta Catarina, Museu Dirings col. [Mar 1956, written in the back of the label] (1♀); [Brazil] Horto Florestal, São Paulo —S.P., Brasil — 13.IV.1962, Lenko & Reichardt col. (2♀); [Brazil] 1.XII.1954, Barueri (Est. S.P.), K. Lenko leg. (1♀); Brasil São Paulo, Est. SP.—J. J. Ferraciolli, leg., 30.II.1954 (1♀); Brasil, Santos, Est[ado] S. Paulo, Dirings col. [Nov 1950, written in the back of the label] (2♀); [Brazil] Horto Florestal, São Paulo —S.P., Brasil — 13.IV.1962, Lenko & Reichardt col. (4♂); Brasil, Joinville, S[an]ta Catarina, Dirings col. [Agt 1956 written in the back of the label] (2♂); Brasil, Santos, Est[ado] S[ão] Paulo, Dirings col. [Nov 1950, written in the back of the label] (1♂); [Brazil] Barueri, São Paulo — Brasil, 28. III.1955, K. Lenko col. (1♂); Brasil, Joinville, S[an]ta Catarina, II-54, Dirings col. (1♂). Itatiaia, E[stado] do Rio-Brasil, J. F. Zikán col., 7.IV.[19]31 (18♀ ♂); Brasil, RJ Estado, Local Ipanema, Col. J. Jurberg, Data X-[19]91, Nº (1♂); Brasil —Estado PR, Local Óleo, Col. Cunha, Data 01/[19]96, Nº (1♂); Brazil, Rio de Jan[eiro], Acc[ess] No. 2966 Nov [ember], CJ Drake coll. 1956 (1♀). [Brazil] Rio [de Janeiro], PR Uhler Collection, no date (1♀ 2♂). * the species D. braziliensis and D. Impicticollis have unique colouration within the genus Dinidor; see text for explanation. ** Except for D. jograziae, body length measurements based on Durai (1987). Comments. The study of the specimens allows us to conclude that the correct identification of the species cited in Genevcius et al. (2016) is actually D. saucius. Some diagnostic characteristics pointed out by Durai (1987) in her key to separate D. mactabilis and D. saucius, for instance the colouration of the costal margin of the corium, are incongruent with the type specimens of both species, and the additional material examined. Indeed, the costal margins of hemelytra in red, orange or light brown is a characteristic seen in every species of the genus Dinidor, and cannot be used to separate the species. Some of the other characters used to diagnose the species, such as the width of the median coloured stripe along the pronotum, showed great variation within the species, and are not reliable for the correct identification of both species, D. mactabilis and D. saucius. Diagnostic characters for the five species of Dinidor with similar colouration (excluding D. braziliensis and D. impicticollis) are presented in Table 1. Prior to this work, the distribution of D. saucius has been scarcely addressed. Stål (1870) described the species from Rio de Janeiro (Brazil).Although Durai (1987) only mention Brazil for the distribution of D. saucius, additional material examined by the author were collected in the neighbouring State of Espírito Santo (dashed in the map), expanding the distribution of the species. Rolston et al. (1996) only cited Rio de Janeiro, and as the two previous works, no specific locality information was provided. Genevcius et al. (2016) recorded D. saucius (as D. mactabilis, see above) from the state of São Paulo (São Bernardo do Campo). Aside from the new locality records from the states of Rio de Janeiro and São Paulo, we extend the known distribution of D. saucius southwards by at least 600km, to the state of Santa Catarina. The distribution pattern of D. saucius seems to include only the Atlantic Rain Forest region, in southern and southeastern Brazil (Fig. 9).
Published as part of López, Guilherme E. L., Carrenho, Renan & Schwertner, Cristiano F., 2021, Description of a new species of Dinidor Latreille (Hemiptera: Dinidoridae) from the Brazilian Amazon Rainforest, and new records of D. saucius Stål, pp. 654-662 in Zootaxa 4958 (1) on pages 658-660, DOI: 10.11646/zootaxa.4958.1.40, http://zenodo.org/record/4692744
{"references":["Stal, C. (1870) Enumeratio Hemipterorum. Bidrag till en foreteckning ofver alla hittils kanda Hemiptera jemte systematiska meddelanden. Enumeratio Dinidorinorum. In: Kungliga Svenska vetenskaps-akademiens handlingar. Bd. 9. No. 1. P. A. Norstedt, Stockholm, pp. 79 - 89.","Schouteden, H. (1913) Heteroptera. Fam. Pentatomidae. Subfam. Dinidorinae, the genera with lists of species. Genera Insectorum, Fasc. 153, 1 - 19, 2 pls.","Durai, P. S. S. (1987) A Revision of the Dinidoridae of the World (Heteroptera: Pentatomoidea). Oriental Insects, 21 (1), 163 - 360. https: // doi. org / 10.1080 / 00305316.1987.11835477","Genevcius, B. C., Carrenho, R. & Schwertner, C. F. (2016) Dinidor mactabilis (Perty, 1833): first record of Dinidoridae (Hemiptera: Pentatomoidea) in the state of Sao Paulo, Brazil. Check List, 12 (3), 1 - 4. https: // doi. org / 10.15560 / 12.3.1900","Rolston, L. H., Rider, D. A., Murray, M. J. & Aalbu, R. L. (1996) Catalog of the Dinidoridae of the world. Papua New Guinea Journal of Agriculture, Forestry and Fisheries, 39 (1), 22 - 101."]}

Biodiversity, Taxonomy, Animalia, Arthropoda, Insecta, Hemiptera, Pentatomidae, Animal Science and Zoology, Ecology, Evolution, Behavior and Systematics, biology.organism_classification, biology, Taxonomy (biology), Zoology, Heteroptera, Pentatominae, and Key (lock)

Weiler, Luciana, Ferrari, Augusto, Grazia, Jocelia (2011): Contributions to the knowledge of Euschistus (Lycipta) with the description of E. (L.) riograndensis sp. nov. (Hemiptera: Heteroptera: Pentatomidae: Pentatominae: Carpocorini). Zootaxa 3067: 59-64, DOI: 10.5281/zenodo.207439
{"references":["Baker, A.D. (1931) A study of the male genitalia of Canadian species of Pentatomidae. Canadian journal of Research, 4, 148- 220.","Dupuis, C. (1970) Heteroptera. In: Tuxen S.L. (Ed.), Taxonomist's glossary of genitalia in insects. Munksgaard, Copenhagen, pp. 190-209.","Ferrari, A.; Schwertner, C.F. & Grazia, J. (2010) Review, cladistic analysis and biogeography of Nezara Amyot & Serville (Hemiptera: Pentatomidae). Zootaxa, 2424, 1-41.","Grazia, J. & Hildebrand, R. (1982) Revisao do genero Berecynthus Stal, 1862 (Heteroptera, Pentatomidae, Pentatomini). Revista Brasileira de Entomologia 26, 173-182.","Grazia, J. & Hildebrand R. (1983) Uma nova especie de Euschistus Dallas, 1851 (Heteroptera, Pentatomidae). Iheringia, Serie Zoologia, 62, 81-88.","Piran, A.A. (1963) Hemiptera neotropica IX. Especies nuevas y no mencionadas para las faunas de Peru, Brasil y Bolivia. Physis, 24(67), 219-222.","Rolston, L.H. (1982) A revision of Euschistus Dallas subgenus Lycipta Stal (Hemiptera: Pentatomidae). Proceedings of the Entomological Society of Washington, 84, 281-296.","Rolston, L.H. (1984) Key to the males of the nominate subgenus of Euschistus in South America, with descriptions of three new species (Hemiptera: Pentatomidae). Journal of the New York Entomological Society, 92, 352-364.","Schaefer, C.W. (1977) Genital capsule of the trichophoran male (Hemiptera: Heteroptera: Geocorisae). International Journal of Insect Morphology & Embryology, 6, 277-301.","Stal, C. (1860-1862a) Bidrag till Rio Janeiro-traktens, Hemipter-fauna. Kongliga Svenska Vetenskaps-Akademiens Handlingar, 2(7), 1-84, 3(6), 1-75.","Stal, C. (1862b) Hemiptera Mexicana enumeravit speciesque novas descripsit. Stettiner Entomologische Zeitung, 23, 81-462.","Stal, C. (1872) Enumeratio Hemipterorum 2. Kongliga Svenska Vetenskaps-Akademiens Handlingar, 10, 1-159.","Thomas, D.B. (1983) A note on the homonymy of Euschistus luridus Dallas 1851. (Hemiptera: Pentatomidae). Proceedings of the Entomological Society of Washington, 85(1), 182.","Weiler, L.M. (2011). Analise cladistica e descricao de uma nova especie para o subgenero Lycipta Stal, 1862 (Hemiptera, Pentatomidae, Carpocorini, Euschistus). Programa de Pos-Graduacao em Biologia Animal, Universidade Federal do Rio Grande do Sul, Porto Alegre. (Dissertacao de Mestrado), 97 pp."]}

Biodiversity, Taxonomy, Animalia, Arthropoda, Insecta, Hemiptera, Dinidoridae, Dinidor, and Dinidor jograziae

Dinidor jograziae sp. nov. (Figs. 1–8) Type material. Holotype (♂): S. Gabriel [Municipality of São Gabriel da Cachoeira], Rio Negro, Amaz. [State of Amazonas], 29.XI.1927, J. F. Zikán col., Coleção J. F. Zikán, 80688, CEIOC. Paratype: 1♀, same label as the holotype, CEIOC. Etymology. The species is named in honour of Prof. Dr. Jocélia Grazia, for her extensive and decisive contributions to the knowledge of the Heteroptera fauna of the Neotropics. Also, we recognize her for being a kind, inspiring and vivid mentor to several generations of taxonomists, including ourselves. Her legacy will always be a part of our lives. Diagnosis. Dinidor jograziae sp. nov. can be recognized by the following combination of characters: mandibular plates relatively short, truncate to rounded at apex, and not contacting each other in front of clypeus; lateral margins of mandibular plates sinuate; first antennal segment exceeds the apex of mandibular plates; rostrum only reaching the anterior margins of mesocoxae; pronotum with a medial suffused red stripe; anterolateral margins of pronotum straight, not emarginate; humeral angles rounded; scutellum with a medial ocher spot along basal margin, and a red/reddish brown suffused patch on the apical tip, reaching the middle of sternite IV, much shorter than half the length of the abdomen; lateral margins of the scutellum sinuate, narrowing posteriorly, apex acuminate; hemelytral membrane dark brown, with darker, reticulate veins; thoracic sterna, coxae and trochanter suffused in reddish brown; connexiva mostly red, with large black spots on anterior and posterior margins; ventral abdomen mostly black, suffused medially with reddish-brown band close to the posterior margin, and a semi-circular red marking on the lateral margin of each urosternite. Description. Measurements (in mm, ♂ / ♀). Total length 14.4/17.0; head length (along midline from base to an imaginary line connecting apices of mandibular plates) 1.6/1.7; head width (measured across eyes) 2.2/2.4; interocular distance 1.7/1.9; interocellar distance 0.8/0.9; lengths of antennal segments: scape 0.8/1.05, pedicel 1.8/2.1, basiflagellum 1.4/1.6, distiflagellum 2.4/2.7; labial segments lengths: AI 1.0/1.0, AII 1.2/1.1, AIII 0.7/0.8, AIV 0.6/0.7; pronotum length (along midline) 3.9/4.5; pronotum width (at humeral angles) 6.9/8.3; scutellum length (along midline) 3.8/4.1; scutellum width (at base) 4.0/5.0; abdomen width 8.2/10.1. General body outline and colour (figs. 1, 2). Elongate, much longer than wide, oval in shape. Abdomen, at its widest point, wider than pronotum. General colour of body black, with many structures (described below) red, and some spots yellow/ocher; venter black, with median portion of the sternites III–VI brown. Lateral paired trichobothria located within large, round, orangish-yellow calluses located on sternites III–VII. Entire body (including legs and antennae) covered with fine yellowish bristles. Head (fig. 3). Relatively short, 0.4 times length of pronotum; wider than longer, width about 1.4 times length. Rugose, mainly on dorsal portion, and even more prevalent on mandibular plates (mp); a few yellow bristles present. Mandibular plates 1.5 times longer than clypeus, apex round to truncate, coming close to each other but not touching at apex; lateral margins of mandibular plates sinuate. Bucculae anteriorly rounded, extending almost to apex of head; posteriorly, bucculae nearly reaching to middle of compound eyes. Compound eyes rounded, positioned laterally, slightly pedunculate, protruding. Ocelli rounded, red to reddish brown, located much closer to compound eyes than to each other. Antennae 4-segmented; first 3 segments concolourous with head; 4th segment mostly ochraceous, with a very small portion of its base concolourous with other segments. Antennal segments I and IV cylindrical; segments II and III prismatic, grooved on dorsal and ventral surfaces. First antennal segment exceeds apex of head; length of segments ratio: IV>II>III>I. Antenniferous tubercles ventrally positioned, partially obscured by compound eyes and mandibular plates. Rostrum 4-segmented, reaching anterior margins of mesocoxae; segment II longest; segment I reaches base of head, longer than segment III, segment IV shortest. Thorax (figs. 1–3). Pronotum trapezoidal, with anterior angles slightly projecting anteriorly. Anterior margin of pronotum concave; anterolateral margins straight, not emarginate; humeral angles rounded. General colour of pronotum black, with lateral margins and a medial stripe suffused red; stripe thin on anterior half of its length, becoming wider posteriorly. Scutellum subtriangular, lateral margins slightly sinuate, narrowing posteriorly, with a small expansion at apex, acuminate; scutellum reaching middle of sternite IV, much shorter than half length of abdomen. Scutellum mostly of same colour as body, with an ochraceous spot at midline of basal margin, and a red/reddish brown suffused patch at apex. Meso- and metasternum with a distinct, deep, medial groove, narrowing between mesocoxae, suffused in reddish brown (along with coxae and trochanters). Hemelytra (fig. 1). Corium longer than scutellum, claval comissure absent; membranes reaching posterior margin of urosternite VII. Corium mostly black, costal margins red, concolorous with lateral margins and medial stripe of pronotum. Membranes dark brown, with many thick, darker veins, forming a reticulate pattern with many closed cells. Legs (fig. 2). Forecoxae much closer to each other than mesocoxae and metacoxae; distance between mesocoxae subequal to distance between metacoxae. Tibiae grooved dorsally; foretibiae slightly depressed laterally. Coxae and trochanters reddish brown, remaining leg segments black. Foretibial apparatus present; tibial combs present. Tarsi 3-segmented, claws depressed laterally, without bristles. Pulvilli differentiated into basi- and distipulvilli. Abdomen (figs. 1, 2, 4). Connexiva mostly exposed, red with large black spots on anterior and posterior margins of each connexival segment; posterolateral angles slightly projected. Spiracle II slightly exposed in male, totally covered by urosternite III in female. Spiracles III–VII exposed, located laterally on urosternites. In females, spiracle exposed on laterotergites VIII. Pseudo-sutures present on each urosternite, posteriorly to spiracles. A pair of trichobothria present on segments III–VII, located on a large, round, orangish-yellow callus, contiguous with pseudo-sutures, oriented diagonally with spiracles of corresponding segments. Abdominal venter mostly black, suffused medially with reddish-brown band close to posterior margin, a semi-circular, red marking on lateral margin of each urosternite. Male genital capsule (figs. 5–8). External opening dorso-posterior; outline of posterior rim oval, wider than long; dorsal rim (dr) folded inwards medially over segment 10 (X), rugose; ventral rim (vr) emarginate, slightly undulated medially, almost straight; cuplike sclerite with a small projection (csp). Parameres (p) each with apical border regular, nearly straight, thin, covered with many yellowish bristles. Female genital plates (figs. 2, 4). Gonocoxites 8 (gc8) subtriangular, wider than long, shorter than laterotergites 8 (lt8); mesial margins juxtaposed, posterior margins slightly convex. Laterotergites 8 triangular, posterior margins slightly concave; spiracles (s) located basally. Laterotergites 9 (lt9) oblong, longer than wider, apices rounded; mesial margins juxtaposed basally, straight, divergent towards apices; posterior margins suffused with red; surface with a small depression laterally. Segment 10 (X) visible ventrally between laterotergites 9. Comments. Dinidor jograziae is easily separated from D. braziliensis and D. impicticollis by the unique body colour patterns of these two species; that is, D. braziliensis is mostly brown in colour, D. impicticollis is the only species with the pronotum entirely black without any spots or stripes of a different colour. In turn, D. jograziae has the same body colour pattern of the remaining described species of Dinidor: mostly black with a red to reddish-brown stripe present along the lateral margins of the pronotum, which extends to the costal margins of the hemelytra. The new species differs from the remaining species (D. mactabilis, D. pulsator, D. rufocinctus and D. saucius) in several aspects including colouration pattern, shape and proportion of the mandibular plates, proportional length of the rostrum, shape of the lateral margins of the pronotum and humeral angles, length and general outline of the scutellum, and general outline of the ventral rim of the pygophore, among others (Table 1). Some of the major differences we found between D. jograziae and the three similar species found in northern South America (D. mactabilis, D. pulsator and D. rufocinctus) include characteristics such as size and the general body outline. Two species, D. mactabilis and D. rufocinctus, are larger than D. jograziae, the anterolateral margins of the pronotum are slightly convex and emarginate, the humeral angles are obtusely rounded, and the connexiva are narrowly exposed, giving the body outline a more elongate aspect. Although D. pulsator is similar in size and has rounded humeral angles, the description provided by Durai (1987) highlights the anterolateral margins of pronotum being strongly reflexed (“tilted upwards”), and the connexiva is narrowly exposed. The new species is smaller, the anterolateral margins of the pronotum are straight, not reflexed, and the connexiva is largely exposed. The colouration pattern of the connexiva also differs between them; for example in D. mactabilis, D. pulsator and D. rufocinctus, it is mostly red to orange-brown coloured with small black or brown spots on the intersegments, while in D. jograziae, it is red with large black spots on both the anterior and posterior margins of each segment. Dinidor jograziae and D. saucius are similar in size, and they share an oval body outline with the humeral angles rounded, and the connexiva largely exposed. Both species also share the coloured pattern of the connexiva. However, D. saucius has the mandibular plates spatulate (truncate in D. jograziae), with lateral margins deeply sinuate (only slightly sinuate in D. jograziae), pronotum with the anterolateral margins slightly concave (straight in D. jograziae), scutellum with the lateral margins deeply sinuous and apex rounded (lateral margins sinuate and apex acuminate in D. jograziae sp. nov.). Also, although variable in width, the median red stripe on the pronotum is always wider and more conspicuous in D. saucius. Additionally, D. saucius seems to be restricted to southeastern Brazil (see discussion below), while D. jograziae appears to be endemic to the Amazon region (Fig. 9). Distribution. BRAZIL: Amazonas (São Gabriel da Cachoeira).
Published as part of López, Guilherme E. L., Carrenho, Renan & Schwertner, Cristiano F., 2021, Description of a new species of Dinidor Latreille (Hemiptera: Dinidoridae) from the Brazilian Amazon Rainforest, and new records of D. saucius Stål, pp. 654-662 in Zootaxa 4958 (1) on pages 655-658, DOI: 10.11646/zootaxa.4958.1.40, http://zenodo.org/record/4692744
{"references":["Durai, P. S. S. (1987) A Revision of the Dinidoridae of the World (Heteroptera: Pentatomoidea). Oriental Insects, 21 (1), 163 - 360. https: // doi. org / 10.1080 / 00305316.1987.11835477"]}

Biodiversity, Taxonomy, Animalia, Arthropoda, Insecta, Hemiptera, Pentatomidae, Rhacognathus, and Rhacognathus americanus

Rhacognathus americanus Stål, 1870 Distribution: Canada: AB, MB, NT (Maw et al. 2000), ON (Maw et al. 2000, Roch 2020), QC (Roch 2020). United States: IL, IN, MA, MI, MN, NE, OH. Comments: Paiero (2019) has recently discussed the known records of this species from North America and has speculated that it might be extirpated from or might even be extinct from large portions of its known range. Further collecting is needed to test this possibility.
Published as part of Rider, David A. & Swanson, Daniel R., 2021, A distributional synopsis of the Pentatomidae (Heteroptera) north of Mexico, including new state and provincial records, pp. 1-69 in Zootaxa 5015 (1) on page 10, DOI: 10.11646/zootaxa.5015.1.1, http://zenodo.org/record/5159085
{"references":["Stal, C. (1870) Enumeratio Hemipterorum. Bidrag till en foreteckning ofver alla hittils kanda Hemiptera, jemte systematiska meddelanden. 1. Kongliga Svenska Vetenskaps-Akademiens Handlingar, 9 (1), 1 - 232. https: // doi. org / 10.5962 / bhl. title. 12549","Maw, H. E. L., Foottit, R. G., Hamilton, K. G. A. & Scudder, G. G. E. (2000) Checklist of the Hemiptera of Canada and Alaska. National Research Council of Canada, NRC Research Press, Ottawa, 220 pp. Canada,","Roch, J-F. (2020) Entomofaune du Quebec. Liste des Punaises du Quebec et des Regions Adjacentes (Hemiptera: Heteroptera). Document Faunique 27. Version 2.3. Entomofaune du Quebec Inc., Saguenay, 41 pp.","Paiero, S. M. (2019) Does it make scents? Making a case for the extirpation, and possibly extinction, of the native stink bug Rhacognathus americanus (Pentatomidae: Asopinae). Journal of the Entomological Society of Ontario, 150, 37 - 45."]}

Myrcia ovata, Lippia sidoides, atividades biol?gica, biological activities, and Qu?mica

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2020-05-18T13:43:55Z No. of bitstreams: 1 2013 - Geovany Amorim Gomes.pdf: 11819103 bytes, checksum: 381e568ac6398f6cfa4544b2f766e74d (MD5) Made available in DSpace on 2020-05-18T13:43:57Z (GMT). No. of bitstreams: 1 2013 - Geovany Amorim Gomes.pdf: 11819103 bytes, checksum: 381e568ac6398f6cfa4544b2f766e74d (MD5) Previous issue date: 2013-12-05 Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior, CAPES, Brasil. The present work reports the phytochemical study of essential oils, and of the hexane and ethyl acetate fractions obtained from ethanolic extract of leaves of Myrcia ovata Cambess. (Myrtaceae), as well as the study of the dichloromethane fraction from the ethanolic extract of stem and branch of this species. In addition, the chemical composition analysis of essential oils from Lippia sidoides Cham. leaves (Verbenaceae) was done. The chromatographic processing of these fractions allowed to the isolation of fifteen non-volatile constituents of M. ovata, including, nine phenolic compounds tectochrysin; 6-methyltectochrysin; 8-methyltectochrysin; 2,5-dihydroxy-6-methyl-7-methoxyflavanone; 2,5-dihydroxy-8-methyl-7-methoxyflavanone; astilbin (rel-2R,3R-,5,7,3?,4?-tetrahydroxy-3-O-?-L-rhamnopyranosylflavanonol); quercetin 3,3?,4?,5,7-pentahydroxyflavone); engeletin (rel-2R,3R-5,7,4?-trihydroxy-3-O-?-L-rhamnopyranosylflavanonol) and 4,5-di-O-caffeoylquinic acid methyl ester, four triterpenes (friedelin, lupeol, ?-and ?-amyrin), one steroid (?-sitosterol) and one sesquiterpene (7-hydroxycadalenal). The reaction of 6-methyltectochrysin with iodine yielded new derivative. The structural formulas were identified by 1H and 13C NMR (1D and 2D), masses, IR and CD spectra data analysis, as well as comparison with literature data. The oxygenated monoterpenes geranial and thymol were prevalent in the essential oils of M. ovata and L. sidoides, respectively. The oils were obtained by hydrodistillation or steam distillation method. The analysis was performed by GC/MS, GC/FID and by retention rates of the constituents by co-injection of hydrocarbon standards. The ethyl acetate fraction of ethanolic extract from leaves of M. ovata, stood out among the rest in relation to antioxidant activity. The presence of quercetin, astilbin, engeletin and 4,5-di-O-caffeoylquinic acid methyl ester in this fraction, can justify this activity. The essential oil of M. ovata presented low antioxidant activity, moderate larvicidal activity against Aedes aegypti L. (Diptera: Culicidae), strong acaricidal action against larvae of Rhipicephalus microplus (Canestrini, 1888) (Acari: Ixodidae) and partial control of engorged females of this species of tick. The oil of L. sidoides showed ninficidal action against Rhodnius prolixus (Stal, 1859) (Hemiptera: Reduviidae), while thymol and carvacrol exhibited biocidal effect in eggs and nymphs of this insect. Furthermore, this oil presented acaricidal activity on larvae of Dermacentor nitens (Neumann, 1897), Amblyomma cajennense (Fabricius, 1787) and R. sanguineus (Latreille, 1806) (Acari: Ixodidae), nymphs of the latter two species of ticks and larvae and engorged females of R. microplus. O presente trabalho relata o estudo fitoqu?mico de ?leos essenciais e de fra??es hexano e acetato de etila obtidas do extrato etan?lico de folhas de Myrcia ovata Cambess. (Myrtaceae), e da fra??o obtida com diclorometano do fracionamento do extrato etan?lico de caule e galhos desta esp?cie. Al?m disso, realizou-se a an?lise da composi??o qu?mica de ?leos essenciais de folhas de Lippia sidoides Cham. (Verbenaceae). O processamento cromatogr?fico destas fra??es forneceram 15 constituintes n?o-vol?teis de M. ovata, destes, nove s?o compostos fen?licos, tectocrisina, 6-metiltectocrisina, 8-metiltectocrisina, 2,5-diidroxi-6-metil-7-metoxiflavanona, 2,5-diidroxi-8-metil-7-metoxiflavanona, astilbina (rel-2R,3S-5,7,3',4'-tetraidroxi-3-O-?-L-ramnopiranosilflavanonol), quercetina (3,3?,4?,5,7-pentahidroxiflavona); engeletina (rel-2R,3S-5,7,4?-triidroxi-3-O-?-L-ramnopiranosilflavanonol) e 4,5-di-O-cafeoilquinato de metila; quatro triterpenos, friedelina, lupeol, ?-e ?-amirina, um esteroide, ?-sitosterol e um sesquiterpeno (7-hidroxicadalenal). A rea??o de ioda??o da flovona 6-metiltectocrisina forneceu o derivado 8-iodo-6-metiltectocrisina, subst?ncia in?dita na literatura. As f?rmulas estruturais foram identificadas atrav?s de an?lise dos dados espectrom?tricos de RMN de 1H e 13C (1D e 2D), massas, IV e DC, al?m de compara??o com dados da literatura. Os monoterpenos oxigenados geranial e timol foram predominantes nos ?leos essenciais de M. ovata e L. sidoides, respectivamente. Os ?leos foram obtidos por hidrodestila??o ou pelo m?todo de arraste a vapor. A an?lise foi realizada atrav?s de CG/EM, CG/DIC e pela obten??o de ?ndices de reten??o dos constituintes atrav?s de co-inje??o de padr?es de hidrocarbonetos. A fra??o acetato de etila do extrato etan?lico de folhas de M. ovata, destacou-se entre as demais em rela??o ? atividade antioxidante. A presen?a de quercetina, astilbina, engeletina e 4,5-di-O-cafeoilquinato de metila nesta fra??o, podem justificar esta atividade. O ?leo essencial de M. ovata apresentou baixo poder antioxidante, moderada atividade larvicida frente ? Aedes aegypti (Linnaeus, 1762) (Diptera: Culicidae), potente a??o carrapaticida frente a larvas de Rhipicephalus microplus (Canestrini, 1888) (Acari: Ixodidae) e controle parcial de f?meas ingurgitadas desta esp?cie de carrapato. O ?leo de L. sidoides apresentou a??o ninficida contra Rhodnius prolixus (Stal, 1859) (Hemiptera: Reduviidae), enquanto timol e carvacrol exibiram efeito biocida em ovos e ninfas deste inseto. Al?m disso, esse ?leo exibiu atividade carrapaticida sobre larvas de Dermacentor nitens (Neumann, 1897), Amblyomma cajennense (Fabricius, 1787) e R. sanguineus (Latreille, 1806) (Acari: Ixodidae), ninfas das duas ?ltimas esp?cies de carrapatos e larvas e f?meas ingurgitadas de R. microplus.

This article documents the addition of 473 microsatellite marker loci and 71 pairs of single-nucleotide polymorphism (SNP) sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Barteria fistulosa, Bombus morio, Galaxias platei, Hematodinium perezi, Macrocentrus cingulum Brischke (a.k.a. M.abdominalis Fab., M.grandii Goidanich or M.gifuensis Ashmead), Micropogonias furnieri, Nerita melanotragus, Nilaparvata lugens Stal, Sciaenops ocellatus, Scomber scombrus, Spodoptera frugiperda and Turdus lherminieri. These loci were cross-tested on the following species: Barteria dewevrei, Barteria nigritana, Barteria solida, Cynoscion acoupa, Cynoscion jamaicensis, Cynoscion leiarchus, Cynoscion nebulosus, Cynoscion striatus, Cynoscion virescens, Macrodon ancylodon, Menticirrhus americanus, Nilaparvata muiri and Umbrina canosai. This article also documents the addition of 116 sequencing primer pairs for Dicentrarchus labrax.

CIENCIAS BIOLOGICAS::ZOOLOGIA::MORFOLOGIA DOS GRUPOS RECENTES [CNPQ], Cicadellinae, Cicadellidae, Hemiptera, Morfologia, Filogenia, Cicadelídeos, and Lissoscarta

Submitted by Alberto Vieira (martins_vieira@ibest.com.br) on 2018-02-07T23:24:09Z No. of bitstreams: 1 492644.pdf: 9684351 bytes, checksum: f9908407ebf5918b6010cce76c8fb41f (MD5) Made available in DSpace on 2018-02-07T23:24:10Z (GMT). No. of bitstreams: 1 492644.pdf: 9684351 bytes, checksum: f9908407ebf5918b6010cce76c8fb41f (MD5) Previous issue date: 1999-12-02 FAPERJ CAPES O gênero neotropical Lissoscarta Stâl é composto por sete espécies conhecidas: L. beckeri Mejdalani & Felix, L. catutara Young, L. nipata Young, L. pebasensis Young, L. pereneensis Young, L. schlingeri Young e L. vespiformis (Fabricius). Essas espécies são muito semelhantes em tennos de coloração e morfologia externa, podendo ser identificadas somente através do estudo da genitália masculina. Os integrantes desse gênero são, aparentemente, mímicos batesianos de vespas da tribo Epiponini (Vespidae: Polistinae). Esse caso de mimetismo envolve não só características morfológicas, mas também aspectos comportamentais. Uma análise filogenética de Lissoscarta (excluindo-se L. pebasensis, conhecida somente por fêmeas), empregando-se os métodos da Sistemática Filogenética (cladística), é apresentada nesta dissertação. A morfologia externa de Lissoscarta, referente aos caracteres utilizados na análise, assim como a morfologia da genitália masculina e a coloração, é detalhadamente descrita. Os grupos-externos, escolhidos pelo critério de similaridade geral com o grupo-interno, foram os seguintes: Amblyscartidia albofasciata (Walker), Dilobopterus exaltatus (Fabricius), Dilobopterus trinotatus (Signoret) e Dilobopterus vicinus (Signoret). A partir da matriz de dados, com 88 caracteres e dez espécies, obteve-se apenas wn diagrama não-enraizado. A condição monofilética de Lissoscarta, subjetivamente baseada na unifonnidade e exclusividade do padrão mimético de suas espécies, é corroborada, sendo agora sustentada por dezesseis sinapomorfias. A presença de uma forte constrição na base do abdome (aspecto singular nos Cicadellini), simulando o pecíolo abdominal das vespas, é uma das mais conspícuas características apomórficas desse gênero. Determinados aspectos possivelmente relacionadas ao mimetismo originaram-se, ao que parece, em Dilobopterus Signoret. Algumas dessas apomorfias são, provavelmente, o resultado de transformações graduais, morfologicamente direcionadas, na linhagem Diloobopterus-lissoscarta. Assim, o gênero neotropical Dilobopterus, com aproximadamente 51 espécies, pode ser um táxon parafilético. Possivelmente, lissoscarta é uma linhagem especializada desse gênero, na qual teriam surgido características miméticas. Os caracteres do pigóforo masculino e do edeago foram os que mais contribuíram para a topologia obtida. Essas estruturas mostram um claro aumento de complexidade morfológica, caracterizado pela variabilidade de formas e pelo surgimento de processos. Por outro lado, os caracteres da morfologia externa e os da coloração foram mais conservativos em Lissoscarta. Esse aspecto pode ser explicado, provavelmente, pelo fato dos últimos caracteres apresentarem condições diretamente relacionadas à composição de um sinal mimético aposemático para o predador. Esse sinal é de um alto valor adaptativo para o mímico, por reduzir o risco de predação. The Neotropical genus Lissoscarta Stâl is composed of seven known species: L. beckeri Mejdalani & Felix, L. catutara Young, L. nipata Young, L. pebasensis Young, L. pereneensis Young, L. schlingeri Young, and L. vespiformis (Fabricius). These species are ve1y similar in terms of color and externai morphology and can be identified only through the study of the male genitalia. The members of this genus are apparently Batesian mimics of wasps of the tribe Epiponini (Vespidae: Polistinae). This case of mimicry involves morphological and behavioral features. A phylogenetic analysis of Lissoscarta (excluding L. pebasensis, known only from females) employing the methodology of Phylogenetic Systematics (cladistics) is presented in this study. The external morphology of Lissoscarta with regard to the characters employed in the analysis, as well as the morphology of the male genitalia and the coloration, is detailly described. The following outgroups were selected through the criterion of general similarity with the ingroup: Amblyscartidia albofasciata (Walker), Dilobopterus exaltatus (Fabricius), Dilobopterus trinotatus (Signoret), and Dilobopterus vicinus (Signoret). Only one unrooted diagram was obtained from the data matrix, which included 88 characters and ten species. The monophyletic condition of Lissoscarta is supported by sixteen synapomorphies. The monophyly of this genus, which was subjectively based on the uniformity and exclusiveness of the mimetic pattem of its species, is thus corroborated. One of the most conspicuous apomorphic features of Lissoscarta is the abdomen strongly constricted at the base (a unique aspect within the Cicadellini), which simulates the abdominal petiole of the wasps. Some mimicry-related features of Lissoscarta apparently originated in Dilobopterus Signoret. Severa] of these apomorphies are probably the result of gradual, morphologically directed transfonnations in the Dilobopterus-Lissoscarta lineage. Tirns, the Neotropical genus Dilobopterus, which has about 5 l species, may be a paraphyletic taxon. Lissoscarta is possibly a specialized lineage of this genus in which mimetic features have arisen. Characters from the male pygofer and from the aedeagus were the ones that contributed mostly to the obtained topology. TI1ese structures show a clear increase in morphological complexity that is characterized by the variability of shapes and development of processes. On the other hand, the characters from the externai morphology and from the coloration were more conservative in Lissoscarta. This aspect may be explained by the direct relationship of states of these characters with the composition of the aposematic mimetic signal to the predator. This signal has a high significance in fitness to the mimic, due to the reduction of the risk of predation.

Cell Biology, Molecular Biology, Biochemistry, Biophysics, Sample preparation, Malondialdehyde, chemistry.chemical_compound, chemistry, Chromatography, Lipid peroxidation, Butanol, Glutathione, High-performance liquid chromatography, Thiobarbituric acid, and Metabolite

Malondialdehyde (MDA) is a widely used marker of oxidative lipid injury whose concentration varies in response to biotic and abiotic stress. Commonly, MDA is quantified as a strong light-absorbing and fluorescing adduct following reaction with thiobarbituric acid (TBA). However, plant tissues in particular contain many compounds that potentially interfere with this reaction and whose concentrations also vary according to the tissue type and stress conditions. As part of our studies into the stress responses of plant tissues, we were interested in developing a rapid, accurate, and robust protocol for MDA analysis using reverse-phased HPLC to avoid these problems with reaction specificity. We demonstrate that a partitioning step into n -butanol during sample preparation is essential and that gradient HPLC analysis is necessary to prevent sample carryover between injections. Furthermore, the starting composition of the mobile phase must be sufficiently hydrophobic to allow direct injection of the n -butanol extracts without peak splitting, tailing, and other artifacts. To minimize analysis times, we used a short, so-called “Rocket” HPLC column and high flow rates. The optimized HPLC separation has a turnaround time of 2.5 min per sample. Butanolic extracts of MDA(TBA) 2 were stable for at least 48 h, and recoveries were linear between 0.38 and 7.5 pmol MDA added. Importantly, this procedure proved to be compatible with existing extraction procedures for l -ascorbate and glutathione analysis in different plant species, allowing multiple “stress metabolite” analyses to be carried out on a single tissue extract.