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FABELLAR, L. T and HEINRICHS, E. A
- Environmental entomology. 13(3):832-837
- Subjects
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Agronomy, agriculture, phytopathology, Agronomie, agriculture, phytopathologie, Ecology, Ecologie, Entomology, Entomologie, Environment, Environnement, Sciences biologiques et medicales, Biological and medical sciences, Sciences biologiques fondamentales et appliquees. Psychologie, Fundamental and applied biological sciences. Psychology, Ecologie animale, vegetale et microbienne, Animal, plant and microbial ecology, Ecologie appliquée, Applied ecology, Ecotoxicologie, effets biologiques de la pollution, Ecotoxicology, biological effects of pollution, Action de la pollution et effets secondaires des pesticides sur les protozoaires et les invertébrés, Effects of pollution and side effects of pesticides on protozoa and invertebrates, Phytopathologie. Zoologie agricole. Protection des cultures et des forets, Phytopathology. Animal pests. Plant and forest protection, Protozoaires. Invertébrés, Protozoa. Invertebrates, Généralités, Generalities, Lutte, Control, Angiospermae, Arachnida, Arthropoda, Gramineae, Insecta, Invertebrata, Monocotyledones, Spermatophyta, Acephate, Araneida, Carbophenothion, Cypermethrine, Cyrthorhinus lividipennis, Delphacidae, Deltamethrine, Dose létale 50, Lethal dose 50, Dosis letal 50, Déprédateur, Pest, Effet secondaire, Secondary effect, Efecto secundario, Endosulfan, Entomophage, Entomophagous, Entomofago, Ethylan, Heteroptera, Homoptera, Insecticide, Lycosa pseudoannulata, Microvelia atrolineata, Nilaparvata lugens, Oryza sativa, Osbac, Plante céréalière, Cereal crop, Prédateur, Predator, Predador, Toxicité, Toxicity, and Toxicidad
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2. The simultaneous assay of chlorophyll and bacteriochlorophyll in natural microbial communities [1984]
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STAL, L. J, VAN GEMERDEN, H, and KRUMBEIN, W. E
- Journal of microbiological methods. 2(6):295-306
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Medical laboratory techniques, Techniques de laboratoire médical, Microbiology, infectious diseases, Microbiologie, maladies infectieuses, Sciences biologiques et medicales, Biological and medical sciences, Sciences biologiques fondamentales et appliquees. Psychologie, Fundamental and applied biological sciences. Psychology, Microbiologie, Microbiology, Bactériologie, Bacteriology, Méthodes bactériologiques et techniques utilisées en bactériologie, Bacteriological methods and techniques used in bacteriology, Bactériochlorophylle, Bacteriochlorophyll, Bactériophéophytine, Bacteriopheophytine, Chlorophylle, Chlorophyll, Communauté microbienne, Communauté, Community, Dosage, Assay, Ecologie, Ecology, Ecosystème, Ecosystem, Environnement, Environment, Microflore, Microflora, Microorganisme, Microorganism, Méthode, Method, Pigment photosynthétique, Photosynthetic pigment, Pigment, Pigments, Simultané, Concurrent, Soufre, and Sulfur
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3. Nitrogen fixation associated with the cyanobacterial mat of a marine laminated microbial ecosystem [1984]
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STAL, L. J, GROSSBERGER, S, and KRUMBEIN, W. E
- Marine biology (Berlin). 82(3):217-224
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Marine biology, limnology, Biologie marine, limnologie, Ecology, Ecologie, Sciences biologiques et medicales, Biological and medical sciences, Sciences biologiques fondamentales et appliquees. Psychologie, Fundamental and applied biological sciences. Psychology, Ecologie animale, vegetale et microbienne, Animal, plant and microbial ecology, Ecologie microbienne, Microbial ecology, Milieux divers (espace extraatmosphérique, air, eau), Various environments (extraatmospheric space, air, water), Bacteria, Bactérie, Cyanobacteria, Eau mer, Seawater, Agua mar, Ecologie, Ecology, Ecologia, Fixation azote, Nitrogen fixation, Mer du Nord, North Sea, Nitrogenase, Zone intertidale, and Intertidal zone
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STAL, L. J and KRUMBEIN, W. E
- Archives of microbiology. 143(1):67-71
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Biotechnology, Biotechnologies, Microbiology, infectious diseases, Microbiologie, maladies infectieuses, Sciences biologiques et medicales, Biological and medical sciences, Sciences biologiques fondamentales et appliquees. Psychologie, Fundamental and applied biological sciences. Psychology, Microbiologie, Microbiology, Bactériologie, Bacteriology, Métabolisme. Enzymes, Metabolism. Enzymes, Biotechnologie, Biotechnology, Recherche fondamentale orientée, Mission oriented research, Biologie des microorganismes d'intérêt industriel confirmé ou potentiel, Biology of microorganisms of confirmed or potential industrial interest, Physiologie et métabolisme, Physiology and metabolism, Bacteria, Oscillatoriaceae, Oscillatoriales, Bactérie, Alternance, Alternancia, Culture microorganisme, Microorganism culture, Cultivo microorganismo, Cyanobacteria, Dosage, Assay, Dosificacion, Enzyme, Enzima, Fixation azote, Nitrogen fixation, Fijacion nitrogenada, Lumière, Light, Luz, Nitrogenase, Obscurité, Obscurity, Oscuridad, Oscillatoria, Oxidoreductase, and Oxidorreductasa
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STAL, L. J and KRUMBEIN, W. E
- Archives of microbiology. 143(1):72-76
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Biotechnology, Biotechnologies, Microbiology, infectious diseases, Microbiologie, maladies infectieuses, Sciences biologiques et medicales, Biological and medical sciences, Sciences biologiques fondamentales et appliquees. Psychologie, Fundamental and applied biological sciences. Psychology, Microbiologie, Microbiology, Bactériologie, Bacteriology, Métabolisme. Enzymes, Metabolism. Enzymes, Biotechnologie, Biotechnology, Recherche fondamentale orientée, Mission oriented research, Biologie des microorganismes d'intérêt industriel confirmé ou potentiel, Biology of microorganisms of confirmed or potential industrial interest, Physiologie et métabolisme, Physiology and metabolism, Bacteria, Oscillatoriaceae, Oscillatoriales, Bactérie, Culture microorganisme, Microorganism culture, Cultivo microorganismo, Cyanobacteria, Enzyme, Enzima, Fixation azote, Nitrogen fixation, Fijacion nitrogenada, Nitrogenase, Oscillatoria, Oxidoreductase, Oxidorreductasa, Oxygène, Oxygen, Protection, Proteccion, Toxicité, Toxicity, and Toxicidad
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STAL, L. J and KRUMBEIN, W. E
- Botanica marina. 28(8):351-365
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Marine biology, limnology, Biologie marine, limnologie, Plant biology and physiology, Biologie et physiologie végétales, Ecology ethology, Écologie, éthologie, Sciences biologiques et medicales, Biological and medical sciences, Sciences biologiques fondamentales et appliquees. Psychologie, Fundamental and applied biological sciences. Psychology, Ecologie animale, vegetale et microbienne, Animal, plant and microbial ecology, Ecologie microbienne, Microbial ecology, Milieux divers (espace extraatmosphérique, air, eau), Various environments (extraatmospheric space, air, water), Bacteria, Bactérie, Caractérisation, Characterization, Caracterizacion, Cyanobacteria, Eau mer, Seawater, Agua mar, Fixation azote, Nitrogen fixation, Fijacion nitrogenada, Microflore, Microflora, Microscopie optique, Optical microscopy, Microscopia optica, Résistance, Resistance, Resistencia, Salinité, Salinity, and Salinidad
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OLIVEIRA, L. F. S, FRUTUOSO E MELO, P. F, LIMA, J. E. P, and STAL, I. L
- Nuclear engineering and design. 90(1):25-41
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Energy, Énergie, Metallurgy, welding, Métallurgie, soudage, Sciences exactes et technologie, Exact sciences and technology, Sciences appliquees, Applied sciences, Energie, Energy, Energie. Utilisation thermique des combustibles, Energy. Thermal use of fuels, Installations de production et de conversion d'énergie: énergie électrique, énergie thermique., Installations for energy generation and conversion: thermal and electrical energy., Centrales nucléaires à fission, Fission nuclear power plants, America, South America, Amérique du Sud, Amérique, America del sur, Analyse, Analysis, Arbre événement, Brésil, Brazil, Brasil, Centrale nucléaire, Nuclear power plant, Planta nuclear, Evaluation probabiliste, Programme ordinateur, Computer program, Risque, Risk, Riesgo, Sécurité, Safety, and Securidad
- Abstract
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Description de la méthodologie utilisée dans le programme d'ordinateur d'analyse d'arbres d'événements pour des évaluations probabilistes de risques. Description détaillée de la structure du programme et de ses entrées et sorties. Discussion de quelques limitations de la version actuelle du programme.
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STAL, L. J and KRUMBEIN, W. E
- Archives of microbiology. 149(1):76-80
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Biotechnology, Biotechnologies, Microbiology, infectious diseases, Microbiologie, maladies infectieuses, Sciences biologiques et medicales, Biological and medical sciences, Sciences biologiques fondamentales et appliquees. Psychologie, Fundamental and applied biological sciences. Psychology, Microbiologie, Microbiology, Bactériologie, Bacteriology, Métabolisme. Enzymes, Metabolism. Enzymes, Bacteria, Cyanobacteria, Bactérie, Azote, Nitrogen, Nitrogeno, Aérobiose, Aerobiosis, Enzyme, Enzima, Fixation azote, Nitrogen fixation, Fijación nitrogeno, Lumière, Light, Luz, Métabolisme, Metabolism, Metabolismo, Nitrogenase, Obscurité, Obscurity, Oscuridad, Oscillatoria, Photosynthèse, Photosynthesis, Fotosíntesis, Respiration cellulaire, Cell respiration, and Respiración celular
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HEYER, H, STAL, L, and KRUMBEIN, W. E
- Archives of microbiology. 151(6):558-564
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Biotechnology, Biotechnologies, Microbiology, infectious diseases, Microbiologie, maladies infectieuses, Sciences biologiques et medicales, Biological and medical sciences, Sciences biologiques fondamentales et appliquees. Psychologie, Fundamental and applied biological sciences. Psychology, Microbiologie, Microbiology, Bactériologie, Bacteriology, Métabolisme. Enzymes, Metabolism. Enzymes, Bacteria, Bactérie, Acétate, Acetate, Acetato, Anaérobiose, Anaerobiosis, Cyanobacteria, Diholoside, Disaccharide, Diholosido, Ethanol, Etanol, Fermentation, Fermentación, Glycogène, Glycogen, Glicógeno, Lactate, Lactates, Lactato, Métabolisme, Metabolism, Metabolismo, Obscurité, Obscurity, Oscuridad, Sulfure, Sulfides, Sulfuro, Tréhalose, Trehalose, Trehalosa, Fermentation hétérolactique, and Oscillatoria limosa
- Abstract
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The marine nitrogen-fixing cyanobacterium Oscillatoria limosa, strain 23 (Oldenburg) was investigated with respect to its dark anaerobic metabolism. As soon as the cells were incubated anaerobically in the dark, they started to ferment. Glycogen was presumably degraded via the heterolactic fermentative pathway. Glycogen-glucose was degraded to equimolar amounts of lactate, ethanol and carbon dioxide. The disaccharide trehalose, which serves as an osmoprotectant in O. limosa, was also catabolized.
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JACKAI, L. E, ATROPO, P. K, and ODEBIYI, J. A
- Crop protection. 8(6):422-428
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Sciences biologiques et medicales, Biological and medical sciences, Sciences biologiques fondamentales et appliquees. Psychologie, Fundamental and applied biological sciences. Psychology, Phytopathologie. Zoologie agricole. Protection des cultures et des forets, Phytopathology. Animal pests. Plant and forest protection, Protozoaires. Invertébrés, Protozoa. Invertebrates, Signalements, symptômes, dégâts, importance économique, surveillance des populations, Records, symptoms, damages, economic importance, population surveys, Angiospermae, Arthropoda, Dicotyledones, Heteroptera, Insecta, Invertebrata, Leguminosae, Spermatophyta, Coreidae, Déprédateur, Pest, Plaga, Plante légumière, Vegetable crop, Planta leguminosa, Relation animal végétal, Animal plant relation, Relación animal vegetal, Rendement, Yield, Rendimiento, Seuil dégât économique, Economic injury level, Umbral daño económico, Seuil intervention, Treatment threshold, Umbral tratamiento, Surveillance population, Population survey, Vigilancia población, Vigna unguiculata, and Clavigralla tomenta sicollis
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STAL, O, WINGREN, S, CARSTENSEN, J, RUTQVIST, L. E, SKOOG, L, KLINTENBERG, C, and NORDENSKJÖLD, B
- European journal of cancer & clinical oncology. 25(2):301-309
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Biotechnology, Biotechnologies, Medical oncology, Cancérologie, Pharmacology drugs, Pharmacologie, galénique, Sciences biologiques et medicales, Biological and medical sciences, Sciences medicales, Medical sciences, Gynecologie. Andrologie. Obstetrique, Gynecology. Andrology. Obstetrics, Pathologie de la glande mammaire, Mammary gland diseases, Tumeurs, Tumors, Anatomopathologie, Pathology, Anatomía patológica, Cytométrie flux, Flow cytometry, Citometría flujo, DNA, Femelle, Female, Hembra, Glande mammaire pathologie, Mammary gland diseases, Glándula mamaria patología, Homme, Human, Hombre, Hormone ovarienne, Ovarian hormone, Hormona ovárica, Hormone stéroïde sexuelle, Sex steroid hormone, Hormona esteroide sexual, Oestrogène, Estrogen, Estrógeno, Phase S, S Phase, Fase S, Ploïdie, Ploidy, Ploidía, Pronostic, Prognosis, Pronóstico, Récepteur hormonal, Hormonal receptor, Receptor hormonal, Tumeur maligne, Malignant tumor, Tumor maligno, Tumeur, and Tumor
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12. Screening of cowpeas for resistance to Clavigralla tomentosicollis Stål (Hemiptera: Coreidae) [1990]
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JACKAI, L. E. N
- Journal of economic entomology. 83(2):300-305
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Agronomy, agriculture, phytopathology, Agronomie, agriculture, phytopathologie, Entomology, Entomologie, Sciences biologiques et medicales, Biological and medical sciences, Sciences biologiques fondamentales et appliquees. Psychologie, Fundamental and applied biological sciences. Psychology, Agronomie. Sciences du sol et productions vegetales, Agronomy. Soil science and plant productions, Génétique et amélioration des plantes d'intérêt économique, Genetics and breeding of economic plants, Sélection variétale. Amélioration spéciale, objectifs d'amélioration, Varietal selection. Specialized plant breeding, plant breeding aims, Résistance aux organismes nuisibles, Pest resistance, Déprédateurs, Pest animals, Africa, Angiospermae, Arthropoda, Dicotyledones, Heteroptera, Insecta, Invertebrata, Leguminosae, Spermatophyta, Afrique, Coreidae, Criblage, Screening, Cernido, Déprédateur, Pest, Plaga, Etude en laboratoire, Laboratory study, Estudio en laboratorio, Etude en serre, Greenhouse study, Estudio en invernadero, Etude sur terrain, Field study, Estudio en campo, Lignée sélection, Breeding line, Línea selección, Méthode, Method, Método, Méthodologie, Methodology, Metodología, Nigéria, Nigeria, Origine végétale, Plant origin, Origen vegetal, Plante légumière, Vegetable crop, Planta leguminosa, Relation animal végétal, Animal plant relation, Relación animal vegetal, Résistance insecte, Insect resistance, Resistencia insecto, Sensibilité résistance, Sensitivity resistance, Sensibilidad resistencia, Vigna unguiculata, Pest resistance, Clavigralla tomentosicollis, and Résistance déprédateur
- Abstract
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Description des procédures de criblage destinées à tester la résistance de nouvelles accessions de niébé, et qui peuvent être appliquées pour le criblage rapide de germoplasmes et la séparation des lignées de sélection.
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SCHOTZKO, D. J and O'KEEFFE, L. E
- Journal of economic entomology. 83(4):1333-1337
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Agronomy, agriculture, phytopathology, Agronomie, agriculture, phytopathologie, Entomology, Entomologie, Sciences biologiques et medicales, Biological and medical sciences, Sciences biologiques fondamentales et appliquees. Psychologie, Fundamental and applied biological sciences. Psychology, Invertebres, Invertebrates, Ecologie animale, vegetale et microbienne, Animal, plant and microbial ecology, Ecologie animale et végétale, Animal and plant ecology, Autoécologie, Autoecology, Animaux, Animals, Protozoa. Invertebrata, Angiospermae, Arthropoda, Dicotyledones, Heteroptera, Insecta, Invertebrata, Leguminosae, Spermatophyta, Déprédateur, Pest, Plaga, Développement, Development, Desarrollo, Etude en laboratoire, Laboratory study, Estudio en laboratorio, Fécondité, Fecundity, Fecundidad, Lens culinaris, Longévité, Longevity, Longevidad, Pentatomidae, Pisum sativum, Plante hôte, Host plant, Planta huesped, Plante légumière, Vegetable crop, Planta leguminosa, Relation animal végétal, Animal plant relation, Relación animal vegetal, Reproduction, Reproducción, and Thyanta pallidovirens
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VILLBRANDT, M, STAL, L. J, BERGMAN, B, and KRUMBEIN, W. E
- Botanica acta. 105(2):90-96
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Plant biology and physiology, Biologie et physiologie végétales, Sciences biologiques et medicales, Biological and medical sciences, Sciences biologiques fondamentales et appliquees. Psychologie, Fundamental and applied biological sciences. Psychology, Microbiologie, Microbiology, Bactériologie, Bacteriology, Métabolisme. Enzymes, Metabolism. Enzymes, Bactérie, Bacteria, Cyanobacteria, Enzyme, Enzima, Activité enzymatique, Enzymatic activity, Actividad enzimática, Inactivation, Inactivación, Localisation, Localization, Localización, Modification, Modificación, Méthode immunoblotting, Immunoblotting assay, Nitrogenase, Technique immunoor, Immunogold labelling, Técnica inmunooro, Variation diurne, Diurnal variation, Variación diurna, Dinitrogénase réductase, Dinitrogénase, and Oscillatoria limosa
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VANHECKE, B, DE SCHEPPER, L, DE CEUNINCK, W, D'HAEGER, V, D'OLIESLAEGER, M, BEYNE, E, STALS, L, and ROGGEN, J
- Quality and reliability of electronic devicesQuality and reliability engineering international. 8(3):253-258
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Control theory, operational research, Automatique, recherche opérationnelle, Sciences exactes et technologie, Exact sciences and technology, Sciences appliquees, Applied sciences, Electronique, Electronics, Electronique des semiconducteurs. Microélectronique. Optoélectronique. Dispositifs à l'état solide, Semiconductor electronics. Microelectronics. Optoelectronics. Solid state devices, Circuits intégrés, Integrated circuits, Conception. Technologies. Analyse fonctionnement. Essais, Design. Technologies. Operation analysis. Testing, Aluminium, Bille, Caractéristique temporelle, Circuit intégré, Contact métal métal, Contrainte thermique, Electrodiffusion, Essai vieillissement accéléré, In situ, Interconnexion, Mesure, Or, and Résistance contact
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JACKAI, L. E. N, INANG, E. E, and NWOBI, P
- Tropical pest management. 38(1):56-60
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Agronomy, agriculture, phytopathology, Agronomie, agriculture, phytopathologie, Entomology, Entomologie, Sciences biologiques et medicales, Biological and medical sciences, Sciences biologiques fondamentales et appliquees. Psychologie, Fundamental and applied biological sciences. Psychology, Invertebres, Invertebrates, Phytopathologie. Zoologie agricole. Protection des cultures et des forets, Phytopathology. Animal pests. Plant and forest protection, Protozoaires. Invertébrés, Protozoa. Invertebrates, Lutte, Control, Lutte intégrée, Integrated pest control, Angiospermae, Arthropoda, Coreidae, Culture tropicale, Tropical crop, Cultivo tropical, Dicotyledones, Déprédateur, Pest, Plaga, Heteroptera, Insecta, Insecticide, Insecticida, Invertebrata, Leguminosae, Lepidoptera, Légumineuse grain, Grain legume, Leguminosa grano, Meliaceae, Plante pesticide, Pesticide crop, Planta plaguicida, Pyralidae, Spermatophyta, Azadirachta indica, Efficacité traitement, Treatment efficiency, Eficacia tratamiento, Extrait tissulaire, Tissue extract, Extracto tisular, Lutte antidéprédateur, Pest management, Manejo de plagas, Maruca testulalis, Origine végétale, Plant origin, Origen vegetal, Produit naturel, Natural product, Producto natural, Vigna unguiculata, Clavigralla tomentosicollis, Insecticide végétal, and Botanical insecticide
- Abstract
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The insecticidal activity of neem, Azadirachta indica A. Juss., was tested on two major cowpea pests using different concentrations of aqueous extracts, and powders of both neem seed and kernel. The pests were larvae of Maruca testulalis Geyer (Lepidoptera: Pyralidae), the legume pod borer and Clavigralla tomentosicollis Stäl (Hemiptera: Coreidae), the cowpea coreid bug. Ife Brown, an improved local cowpea variety, and IT84S-2246, a breeding line from IITA grain legume programme, were used in the study. Neem proved effective in acting as an insecticide and affected the rates of development of both pests at concentration as low as ca. 9% solution (wt/vol.).
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VANHECKE, B, DE SCHEPPER, L, DE CEUNINCK, W, D'HAEGER, V, D'OLIESLAEGERS, M, BEYNE, E, ROGGEN, J, and STALS, L
- Microelectronics and reliability. 33(8):1141-1157
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Electronics, Electronique, Sciences exactes et technologie, Exact sciences and technology, Sciences appliquees, Applied sciences, Electronique, Electronics, Electronique des semiconducteurs. Microélectronique. Optoélectronique. Dispositifs à l'état solide, Semiconductor electronics. Microelectronics. Optoelectronics. Solid state devices, Circuits intégrés, Integrated circuits, Conception. Technologies. Analyse fonctionnement. Essais, Design. Technologies. Operation analysis. Testing, Aluminium, Aluminio, Circuit intégré, Integrated circuit, Circuito integrado, Electrodiffusion, Electrodifusión, Encapsulation, Encapsulación, Etude expérimentale, Experimental study, Estudio experimental, Fiabilité, Reliability, Fiabilidad, Interconnexion, Interconnection, Interconección, Métallisation, Metallizing, Metalización, Or, Gold, Oro, Phénomène transport, Transport process, and Fenómeno transporte
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18. S-phase fraction and survival benefit from adjuvant chemotherapy or radiotherapy of breast cancer [1994]
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STÅL, O, SKOOG, L, RUTQVIST, L. E, CARSTENSEN, J. M, WINGREN, S, SULLIVAN, S, ANDERSSON, C, DUFMATS, M, and NORDENSKJÖLD, B
- British journal of cancer. 70(6):1258-1262
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Medical oncology, Cancérologie, Sciences biologiques et medicales, Biological and medical sciences, Sciences medicales, Medical sciences, Pharmacologie. Traitements medicamenteux, Pharmacology. Drug treatments, Anticancéreux, Antineoplastic agents, Chimiothérapie, Chemotherapy, Anticancéreux, Antineoplastic agent, Anticanceroso, Chimiothérapie, Chemotherapy, Quimioterapia, Cycle cellulaire, Cell cycle, Ciclo celular, Cytométrie flux, Flow cytometry, Citometría flujo, Etude cas, Case study, Estudio caso, Exploration, Exploración, Glande mammaire, Mammary gland, Glándula mamaria, Homme, Human, Hombre, Phase S, S Phase, Fase S, Pronostic, Prognosis, Pronóstico, Préménopause, Premenopause, Premenopausia, Radiothérapie, Radiotherapy, Radioterapia, Traitement adjuvant, Adjuvant treatment, Tratamiento adyuvante, Traitement, Treatment, Tratamiento, Tumeur maligne, Malignant tumor, and Tumor maligno
- Abstract
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Cancer chemotherapy interacts with cell proliferation, but data on the relationship between cancer cell replication and the effect of adjuvant chemotherapy are scarce. We have investigated the S-phase fractions of the primary tumour from premenopausal breast cancer patients who participated in a randomised trial comparing 12 cycles of polychemotherapy (CMF) with post-operative radiotherapy. DNA flow cytometry was performed on frozen tissues from 208 primary breast carcinomas, of which the S-phase fraction was estimated in 176 cases. There was a significantly higher benefit from CMF among patients with a high S-phase fraction (P=0.0033).
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19. Tumor necrosis factor alpha promotes replication and pathogenicity of rat cytomegalovirus [1994]
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HAAGMANS, B. L, STALS, F. S, VAN DER MEIDE, P. H, BRUGGEMAN, C. A, HORZINEK, M. C, and SCHIJNS, V. E. C. J
- Journal of virology. 68(4):2297-2304
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Biochemistry, molecular biology, biophysics, Biochimie, biologie moléculaire, biophysique, Microbiology, infectious diseases, Microbiologie, maladies infectieuses, Virology, Virologie, Sciences biologiques et medicales, Biological and medical sciences, Sciences medicales, Medical sciences, Pathologie infectieuse, Infectious diseases, Viroses, Viral diseases, Viroses expérimentales et modèles, Experimental viral diseases and models, Betaherpesvirinae, Cytokine, Citoquina, Herpesviridae, Infection, Infección, Mammalia, Rodentia, Vertebrata, Virus, Cytomegalovirus, Facteur nécrose tumorale α, Tumor necrosis factor α, Factor necrosis tumoral α, Histopathologie, Histopathology, Histopatología, Immunodépression, Immunosuppression, Inmunodepresión, Modèle animal, Animal model, Modelo animal, Multiplication virus, Virus multiplication, Multiplicación virus, Pathogénie, Pathogenesis, Patogenia, Pathologie expérimentale, Experimental disease, Patología experimental, Rat, Rata, Relation hôte virus, Host virus relation, Relación huesped virus, Spécificité tissu, Tissue specificity, Especificidad tejido, Symptomatologie, Symptomatology, Sintomatología, Virose, Viral disease, and Virosis
- Abstract
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We investigated the role of tumor necrosis factor alpha (TNF-α) in the pathogenesis of rat cytomegalovirus (RCMV) infection. TNF-α levels found in the sera of radiation-immunosuppressed rats in the course of infection (>350 pg/ml) correlated with the development of RCMV disease. Administration of anti-TNF-α antibodies strongly reduced the severity of pneumonia and led to a reduction in virus titers. In immunocompetent rats, anti-TNF-α antibodies also significantly suppressed viral replication. Conversely, administration of TNF-α augmented RCMV replication and aggravated the disease signs. In vitro, TNF-α enhanced RCMV replication in the macrophage, whereas a reduction of viral replication was observed in fibroblasts, indicating that the effect on viral replication is cell type specific.
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HAAGMANS, B. L, VAN DER MEIDE, P. H, STALS, F. S, VAN DER EERTWEGH, A. J. M, CLAASSEN, E, BRUGGEMAN, C. A, HORZINEK, M. C, and SCHIJNS, V. E. C. J
- Journal of virology. 68(4):2305-2312
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Biochemistry, molecular biology, biophysics, Biochimie, biologie moléculaire, biophysique, Microbiology, infectious diseases, Microbiologie, maladies infectieuses, Virology, Virologie, Sciences biologiques et medicales, Biological and medical sciences, Sciences biologiques fondamentales et appliquees. Psychologie, Fundamental and applied biological sciences. Psychology, Microbiologie, Microbiology, Virologie, Virology, Cycle de développement, interférence, relations hôte-virus, pouvoir pathogène, souches diverses, Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains, Betaherpesvirinae, Cytokine, Citoquina, Herpesviridae, Infection, Infección, Mammalia, Rodentia, Vertebrata, Virus, Activité biologique, Biological activity, Actividad biológica, Cytomegalovirus, In vitro, In vivo, Interféron gamma, Gamma interferon, Interferón gamma, Modèle animal, Animal model, Modelo animal, Multiplication virus, Virus multiplication, Multiplicación virus, Pathologie expérimentale, Experimental disease, Patología experimental, Rat, Rata, Relation hôte virus, Host virus relation, Relación huesped virus, Virose, Viral disease, and Virosis
- Abstract
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The role of gamma interferon (IFN-γ) in the resolution of rat cytomegalovirus (RCMV) infection was investigated. In the spleen, IFN-γ-producing cells reached maximum numbers on day 7 after infection. Prophylactic treatment with high doses of recombinant rat IFN-γ exerted antiviral activity in fibroblasts and protected immunosuppressed rats against a lethal RCMV challenge. Remarkably, in immunocompetent rats, neutralization of endogenous IFN-γ activity significantly reduced the numbers of RCMV antigen-expressing cells in the spleen, the predominant site of viral replication. Moreover, protection of radiation-immunosuppressed infected rats by transferred immune T cells was enhanced by coinjection of IFN-γ neutralizing antibodies.
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