Abstract
Abstract:
Byline: Sheng Yan, Sheng Yan, Shi-Yang Tang, Yuxing Li, Qianbin Zhao, Dan Yuan, Guolin Yun, Jun Zhang, Shiwu Zhang, Shiwu Zhang Keywords: Capillary microfluidics; Laser ablation; Rapid prototyping; Ur Abstract Proteinuria is an established risk marker for progressive renal function loss and patients would significantly benefit from a point-of-care testing. Although extensive work has been done to develop the microfluidic devices for the detection of urinary protein, they need the complicated operation and bulky peripherals. Here, we present a rapid, maskless 3D prototyping for fabrication of capillary fluidic circuits using laser engraving. The capillary circuits can be fabricated in a short amount of time (<10 min) without the requirements of clean-room facilities and photomasks. The advanced capillary components (e.g., trigger valves, retention valves and retention bursting valves) were fabricated, enabling the sequential liquid delivery and sample-reagent mixing. With the integration of smartphone-based detection platform, the microfluidic device can quantify the urinary protein via a colorimetric analysis. By eliminating the bulky and expensive equipment, this smartphone-based detection platform is portable for on-site quantitative detection. Article Note: These authors contributed equally to this work. Additional corresponding author: Prof. Weihua Li E-mail: weihuali@uow.edu.au Color Online: See the article online to view Figs. 1-5 in color. CAPTION(S): Supporting information
