Cell-free prototyping enables implementation of optimized reverse β-oxidation pathways in heterotrophic and autotrophic bacteria

Authors:: Bastian Vögeli
Luca Schulz
Shivani Garg
Katia Tarasava
James M. Clomburg
Seung Hwan Lee
Aislinn Gonnot
Elamar Hakim Moully
Blaise R. Kimmel
Loan Tran
Hunter Zeleznik
Steven D. Brown
Sean D. Simpson
Milan Mrksich
Ashty S. Karim
Ramon Gonzalez
Michael Köpke
Michael C. Jewett
Source:: Nature Communications, Vol 13, Iss 1, Pp 1-10 (2022)
Publication Date:: 2022-06-01
Language:: English
Abstract:: An attractive route for carbon-negative synthesis of biochemical products is the reverse β-oxidation pathway coupled to the Wood-Ljungdahl pathway. Here the authors use a high-throughput in vitro prototyping workflow to screen 762 unique pathway combinations using cell-free extracts tailored for r-BOX to identify enzyme sets for enhanced product selectivity.
Subjects:: Science
Format:: Academic Journal
DOI:: 10.1038/s41467-022-30571-6
Database:: Directory of Open Access Journals
Journal:: Nature Communications
Volume:: 13
Issue:: 1
Page Start:: 1
Page Count:: 10
ISSN:: 20411723
Publisher:: Nature Portfolio, 2022.
Document Type:: article

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