The combined use of ultrasound and molecular sieves improves the synthesis of ethyl butyrate catalyzed by immobilized Thermomyces lanuginosus lipase
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- Authors:
- PALUDO, Natalia
ALVES, Joana S
ALTMANN, Cintia
AYUB, Marco A. Z
FERNANDEZ-LAFUENTE, Roberto
RODRIGUES, Rafael C - Author Affiliations:
- Biotechnology, Bioprocess and Biocatalysis Group, Institute of Food Science and Technology, Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500, P.O. Box 15090, ZC 91501-970 Porto Alegre, RS, Brazil
Department of Biocatalysis, ICP - CSIC, Campus UAM-CSIC, Cantoblanco, ZC 28049 Madrid, Spain - Source:
- Ultrasonics sonochemistry. 22:89-94
- Publication Date:
- 2015-01-01
- Language:
- English
- Abstract:
- In this work, the combined use of ultrasound energy and molecular sieves was investigated for the synthesis of ethyl butyrate, ester with mango and banana notes, catalyzed by the immobilized lipase from Thermomyces lanuginosus (Lipozyme TL-IM). Initially, the best concentrations of biocatalysts (35%) and butyric acid (0.7 M) were tested using ultrasound as an alternative to mechanical agitation. The amount of acid in the reaction could be increased by 2-fold when compared to previous works where mechanical agitation was used. In the next step, substrate molar ratio and reaction temperature were optimized and the best conditions were at their lowest levels: 1:1 (acid:alcohol), and 30 °C, reaching 61% of conversion in 6 h. Molecular sieves (3 A) were added to optimized reaction medium in order to remove the formed water and improve the maximum yield. The reaction yield increased 1.5 times, reaching 90% of conversion in 6 h, when 60 mg of molecular sieves per mmol of butyric acid was used. Finally, the reuse of Lipozyme TL-IM for the ultrasound-assisted synthesis of ethyl butyrate was verified for 10 batches, without any appreciable loss of activity, whereas in systems using mechanical agitation, the biocatalyst was completely inactivated after 5 batches. These results suggest that the combined use of ultrasound and molecular sieves greatly improve esterification reactions by stabilizing the enzyme and increasing yields.
- Notes:
- General chemistry and physical chemistry
- Subjects:
- General chemistry, physical chemistry
Chimie générale, chimie physique
Sciences exactes et technologie
Exact sciences and technology
Chimie
Chemistry
Chimie generale et chimie physique
General and physical chemistry
Théorie des réactions, cinétique générale. Catalyse. Nomenclature, documentation chimique, informatique chimique
Theory of reactions, general kinetics. Catalysis. Nomenclature, chemical documentation, computer chemistry
Catalyse
Catalysis
Physicochimie des réactions induites par rayonnements, particules et ultrasons
Physical chemistry of induced reactions (with radiations, particles and ultrasonics)
Sonochimie
Ultrasonic chemistry
Catalyse hétérogène
Heterogeneous catalysis
Catálisis heterogénea
Enzyme immobilisée
Immobilized enzyme
Enzima inmovilizada
Estérification
Esterification
Esterificación
Réaction catalytique
Catalytic reaction
Reacción catalítica
Sonochimie
Sonochemistry
Sonoquímica
Stabilité
Stability
Estabilidad
Synthèse
Synthesis
Síntesis
Tamis moléculaire
Molecular sieve
Tamiz molecular
Ultrason
Ultrasound
Ultrasonido
Butyrique acide ester éthyle
Esterification reactions
Lipases
Lipozyme TL-IM
Molecular sieves
Operational stability - Format:
- Academic Journal
- Database:
- PASCAL Archive
- Journal:
- Ultrasonics sonochemistry
- Volume:
- 22
- Page Start:
- 89
- Page Count:
- 6
- ISSN:
- 13504177
- Publisher:
- Kidlington: Elsevier, 2015.
- Document Type:
- Article
- Physical Description:
- print, 43 ref
