Rapid Prototyping of Concave Microwells for the Formation of 3D Multicellular Cancer Aggregates for Drug Screening
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- Authors:
- TU, Ting-Yuan
ZHE WANG
JING BAI
WEI SUN
WENG KUNG PENG
HUANG, Ruby Yun-Ju
THIERY, Jean-Paul
KAMM, Roger D - Author Affiliations:
- BioSystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology (SMART) Center, 1 CREATE Way, #04-13/14 Enterprise Wing, Singapore, 138602, Singapore
Cancer Science Institute of Singapore, National University of Singapore MD6, Medical Drive, Singapore, 117456, Singapore
Institute of Molecular Cell Biology (IMCB), A-STAR Department of Biochemistry School of Medicine, National University of Singapore, Proteos, 61 Biopolis Drive, Singapore, 138673, Singapore
Department of Biological Engineering and Department of Mechanical Engineering Massachusetts Institute of Technology, Cambridge, MA 02139, United States - Source:
- Advanced healthcare materials (Print). 3(4):609-616
- Publication Date:
- 2014-01-01
- Language:
- English
- Abstract:
- Microwell technology has revolutionized many aspects of in vitro cellular studies from 2D traditional cultures to 3D in vivo-like functional assays. However, existing lithography-based approaches are often costly and time-consuming. This study presents a rapid, low-cost prototyping method of CO2 laser ablation of a conventional untreated culture dish to create concave microwells used for generating multicellular aggregates, which can be readily available for general laboratories. Polymethylmethacrylate (PMMA), polydimethylsiloxane (PDMS), and polystyrene (PS) microwells are investigated, and each produces distinctive microwell features. Among these three materials, PS cell culture dishes produce the optimal surface smoothness and roundness. A549 lung cancer cells are grown to form cancer aggregates of controllable size from ≈40 to ≈80 μm in PS microwells. Functional assays of spheroids are performed to study migration on 2D substrates and in 3D hydrogel conditions as a step towards recapitulating the dissemination of cancer cells. Preclinical anti-cancer drug screening is investigated and reveals considerable differences between 2D and 3D conditions, indicating the importance of assay type as well as the utility of the present approach.
- Notes:
- Surgery (generalities). Transplants, organs and tissues grafting. Graft pathologies<br><br>Tumours
- Subjects:
- Biomedical engineering
Génie biomédical
Sciences biologiques et medicales
Biological and medical sciences
Sciences medicales
Medical sciences
Tumeurs
Tumors
Tumeurs multiples. Tumeurs solides. Tumeurs chez l'enfant (généralités)
Multiple tumors. Solid tumors. Tumors in childhood (general aspects)
Chirurgie (generalites). Transplantations, greffes d'organes et de tissus. Pathologie des greffons
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Technologie. Biomatériaux. Equipements
Technology. Biomaterials. Equipments
Cancer
Cáncer
Agrégat
Aggregate
Agregado
Criblage
Screening
Cernido
Dépistage
Medical screening
Descubrimiento
Médicament
Drug
Medicamento
Prototypage rapide
Rapid prototyping
Prototipificación rápida
Tumeur maligne
Malignant tumor
Tumor maligno - Format:
- Academic Journal
- Database:
- PASCAL Archive
- Journal:
- Advanced healthcare materials (Print)
- Volume:
- 3
- Issue:
- 4
- Page Start:
- 609
- Page Count:
- 8
- ISSN:
- 21922640
- Publisher:
- Weinheim: Wiley-VCH, 2014.
- Document Type:
- Article
- Physical Description:
- print, 32 ref
