A technology platform for the fast production of monoclonal recombinant antibodies against plant proteins and peptides.
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- Authors:
- Eeckhout D
De Clercq A
Van De Slijke E
Van Leene J
Stals H
Casteels P
Persiau G
Vercammen D
Van Breusegem F
Zabeau M
Inzé D
Jespers L
Depicker A
De Jaeger G - Source:
-
Journal of immunological methods [J Immunol Methods] 2004 Nov; Vol. 294 (1-2), pp. 181-7. - Publication Date:
- 2004-11-01
- Language:
- English
- Abstract:
- The application of recombinant antibodies in plant biology research is limited because plant researchers have minimal access to high-quality phage display libraries. Therefore, we constructed a library of 1.3 x 10(10) clones displaying human single-chain variable fragments (scFvs) that is available to the academic community. The scFvs selected from the library against a diverse set of plant proteins showed moderate to high antigen-binding affinity together with high specificity. Moreover, to optimize an scFv as immunodetection agent, two expression systems that allow efficient production and purification of bivalent scFv-Fc and scFv-CkappaZIP fusion proteins were integrated. We are convinced that this antibody platform will further stimulate applications of recombinant antibodies such as the diagnostic detection or immunomodulation of specific antigens in plants.
- Subjects:
- Antibodies, Monoclonal immunology
Antibody Affinity immunology
Carrier Proteins genetics
Gene Expression
Humans
Immunoglobulin Variable Region immunology
Immunoglobulin Variable Region isolation purification
Immunoglobulin kappa-Chains genetics
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins immunology
Recombinant Fusion Proteins isolation purification
Substrate Specificity genetics
Substrate Specificity immunology
Antibodies, Monoclonal genetics
Antibody Affinity genetics
Gene Library
Immunoglobulin Variable Region genetics
Peptides immunology
Plant Proteins immunology - Format:
- Academic Journal
- DOI:
- 10.1016/j.jim.2004.08.006
- Database:
- MEDLINE
- Journal:
- Journal of immunological methods
- Volume:
- 294
- Issue:
- 1-2
- Page Start:
- 181
- ISSN:
- 0022-1759
