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Book
online resource (xi, 395 pages) : illustrations (some color)
  • Time-lapse fluorescence microscopy of budding yeast cells / Arun Kumar and Manuel Mendoza
  • Real-time visualization and quantification of contractile ring proteins in single living cells / Reshma Davidson ... [et al.]
  • Fluorescence recovery after photo-bleaching (FRAP) and fluorescence loss in photo-bleaching (FLIP) experiments to study protein dynamics during budding yeast cell division / Alessio Bolognesi ... [et al.]
  • High-speed super-resolution imaging of live fission yeast cells / Caroline Laplante ... [et al.]
  • Monitoring chitin deposition during septum assembly in budding yeast / Irene Arcones and Cesar Roncero
  • Imaging septum formation by fluorescence microscopy / Juan Carlos Ribas and Juan Carlos G. Cortés
  • Visualization of cytokinesis events in budding yeast by transmission electron microscopy / Franz Meitinger and Gislene Pereira
  • Visualization of fission yeast cells by transmission electron microscopy / Matthias Sipiczki
  • Characterization of septin ultrastructure in budding yeast using electron tomography / Aurélie Bertin and Eva Nogales
  • Isolation of cytokinetic actomyosin rings from Saccharomyces cerevisiae and Schizosaccharomyces pombe / Junqi Huang ... [et al.]
  • Measurements of myosin-II motor activity during cytokinesis in fission yeast / Qing Tang, Luther W. Pollard, and Matthew Lord
  • In vitro biochemical characterization of cytokinesis actin-binding proteins / Dennis Zimmermann ... [et al.]
  • Characterization of cytokinetic F-BARs and other membrane-binding proteins / Nathan A. McDonald and Kathleen L. Gould
  • Analysis of three-dimensional structures of exocyst components / Johannes Lesigang and Gang Dong
  • Analysis of Rho-GTPase activity during budding yeast cytokinesis / Masayuki Onishi and John R. Pringle
  • Detection of phosphorylation status of cytokinetic components / Franz Meitinger, Saravanan Palani, and Gislene Pereira
  • Studying protein-protein interactions in budding yeast using co-immunoprecipitation / Magdalena Foltman and Alberto Sanchez-Diaz
  • Conditional budding yeast mutants with temperature-sensitive and auxin-inducible degrons for screening of suppressor genes / Asli Devrekanli and Masato T. Kanemaki
  • Synchronization of the budding yeast Saccharomyces cerevisiae / Magdalena Foltman, Iago Molist, and Alberto Sanchez-Diaz
  • Fission yeast cell cycle synchronization methods / Marta Tormos-Pérez, Livia Pérez-Hidalgo, and Sergio Moreno
  • Review of fluorescent proteins for use in yeast / Maja Bialecka-Fornal, Tatyana Makushok, and Susanne M. Rafelski
  • Visualization and image analysis of yeast cells / Steve Bagley
  • Toolbox for protein structure prediction / Daniel Barry Roche and Liam James McGuffin
  • From structure to function : a comprehensive compendium of tools to unveil protein domains and understand their role in cytokinesis / Sergio A. Rincon and Anne Paoletti.
  • Time-lapse fluorescence microscopy of budding yeast cells / Arun Kumar and Manuel Mendoza
  • Real-time visualization and quantification of contractile ring proteins in single living cells / Reshma Davidson ... [et al.]
  • Fluorescence recovery after photo-bleaching (FRAP) and fluorescence loss in photo-bleaching (FLIP) experiments to study protein dynamics during budding yeast cell division / Alessio Bolognesi ... [et al.]
  • High-speed super-resolution imaging of live fission yeast cells / Caroline Laplante ... [et al.]
  • Monitoring chitin deposition during septum assembly in budding yeast / Irene Arcones and Cesar Roncero
  • Imaging septum formation by fluorescence microscopy / Juan Carlos Ribas and Juan Carlos G. Cortés
  • Visualization of cytokinesis events in budding yeast by transmission electron microscopy / Franz Meitinger and Gislene Pereira
  • Visualization of fission yeast cells by transmission electron microscopy / Matthias Sipiczki
  • Characterization of septin ultrastructure in budding yeast using electron tomography / Aurélie Bertin and Eva Nogales
  • Isolation of cytokinetic actomyosin rings from Saccharomyces cerevisiae and Schizosaccharomyces pombe / Junqi Huang ... [et al.]
  • Measurements of myosin-II motor activity during cytokinesis in fission yeast / Qing Tang, Luther W. Pollard, and Matthew Lord
  • In vitro biochemical characterization of cytokinesis actin-binding proteins / Dennis Zimmermann ... [et al.]
  • Characterization of cytokinetic F-BARs and other membrane-binding proteins / Nathan A. McDonald and Kathleen L. Gould
  • Analysis of three-dimensional structures of exocyst components / Johannes Lesigang and Gang Dong
  • Analysis of Rho-GTPase activity during budding yeast cytokinesis / Masayuki Onishi and John R. Pringle
  • Detection of phosphorylation status of cytokinetic components / Franz Meitinger, Saravanan Palani, and Gislene Pereira
  • Studying protein-protein interactions in budding yeast using co-immunoprecipitation / Magdalena Foltman and Alberto Sanchez-Diaz
  • Conditional budding yeast mutants with temperature-sensitive and auxin-inducible degrons for screening of suppressor genes / Asli Devrekanli and Masato T. Kanemaki
  • Synchronization of the budding yeast Saccharomyces cerevisiae / Magdalena Foltman, Iago Molist, and Alberto Sanchez-Diaz
  • Fission yeast cell cycle synchronization methods / Marta Tormos-Pérez, Livia Pérez-Hidalgo, and Sergio Moreno
  • Review of fluorescent proteins for use in yeast / Maja Bialecka-Fornal, Tatyana Makushok, and Susanne M. Rafelski
  • Visualization and image analysis of yeast cells / Steve Bagley
  • Toolbox for protein structure prediction / Daniel Barry Roche and Liam James McGuffin
  • From structure to function : a comprehensive compendium of tools to unveil protein domains and understand their role in cytokinesis / Sergio A. Rincon and Anne Paoletti.
Medical Library (Lane)
Status of items at Medical Library (Lane)
Medical Library (Lane) Status
Check Lane Library catalog for status
SPRINGER PROTOCOLS Unknown
Video
1 online resource (1 streaming video file (40 min.) : color, sound).
  • Contents: Patterns of genetic variation in Europe and the Neolithic
  • Ancient DNA and anatomically modern humans (Challenges & Potential)
  • The Neolithic transition in Europe (Scandinavia, Iberia and Eastern Europe).
  • Contents: Patterns of genetic variation in Europe and the Neolithic
  • Ancient DNA and anatomically modern humans (Challenges & Potential)
  • The Neolithic transition in Europe (Scandinavia, Iberia and Eastern Europe).
Book
online resource (xii, 252 pages) : illustrations (some color)
  • Library construction for mutation identification by whole-genome sequencing / Harold E. Smith
  • Fundamentals of comparative genome analysis in caenorhabditis nematodes / Eric S. Haag and Cristel G. Thomas
  • Genetic methods for cellular manipulations in C. elegans / Menachem Katz
  • Fusion PCR method for expressing genetic tools in C. elegans / Yifat Eliezer and Alon Zaslaver
  • Transposon-assisted genetic engineering with Mos1- mediated single-copy insertion (MosSCI) / Christian Frøkjær-Jensen
  • Creating genome modifications in C. elegans using the CRISPR/Cas9 system / John A. Calarco and Ari E. Friedland
  • Observing and quantifying fluorescent reporters / Michael Hendricks
  • Microbial rhodopsin optogenetic tools : application for analyses of synaptic transmission and of neuronal network activity in behavior / Caspar Glock, Jatin Nagpal, and Alexander Gottschalk
  • Simultaneous optogenetic stimulation of individual pharyngeal neurons and monitoring of feeding behavior in intact C. elegans / Nicholas F. Trojanowski and Christopher Fang-Yen
  • High-pressure freeze and freeze substitution electron microscopy in C. elegans / Laura Manning and Janet Richmond
  • Electron tomography methods for C. elegans / David H. Hall and William J. Rice
  • Microfluidic devices for behavioral analysis, microscopy, and neuronal imaging in Caenorhabditis elegans / Ross C. Lagoy and Dirk R. Albrecht
  • Tracking single C. elegans using a USB microscope on a motorized stage / Eviatar I. Yemini and André E. X. Brown
  • Imaging system for C. elegans behavior / Matthew A. Churgin and Christopher Fang-Yen
  • Method for obtaining large populations of synchronized Caenorhabditis elegans dauer larvae / Maria C. Ow and Sarah E. Hall
  • Sampling and isolation of C. elegans from the natural habitat / Nausicaa Poullet and Christian Braendle
  • Primer on prototyping / Dylan Lynch and David Biron
  • Primer on quantitative modeling / Iulia Neagu and Erel Levine.
  • Library construction for mutation identification by whole-genome sequencing / Harold E. Smith
  • Fundamentals of comparative genome analysis in caenorhabditis nematodes / Eric S. Haag and Cristel G. Thomas
  • Genetic methods for cellular manipulations in C. elegans / Menachem Katz
  • Fusion PCR method for expressing genetic tools in C. elegans / Yifat Eliezer and Alon Zaslaver
  • Transposon-assisted genetic engineering with Mos1- mediated single-copy insertion (MosSCI) / Christian Frøkjær-Jensen
  • Creating genome modifications in C. elegans using the CRISPR/Cas9 system / John A. Calarco and Ari E. Friedland
  • Observing and quantifying fluorescent reporters / Michael Hendricks
  • Microbial rhodopsin optogenetic tools : application for analyses of synaptic transmission and of neuronal network activity in behavior / Caspar Glock, Jatin Nagpal, and Alexander Gottschalk
  • Simultaneous optogenetic stimulation of individual pharyngeal neurons and monitoring of feeding behavior in intact C. elegans / Nicholas F. Trojanowski and Christopher Fang-Yen
  • High-pressure freeze and freeze substitution electron microscopy in C. elegans / Laura Manning and Janet Richmond
  • Electron tomography methods for C. elegans / David H. Hall and William J. Rice
  • Microfluidic devices for behavioral analysis, microscopy, and neuronal imaging in Caenorhabditis elegans / Ross C. Lagoy and Dirk R. Albrecht
  • Tracking single C. elegans using a USB microscope on a motorized stage / Eviatar I. Yemini and André E. X. Brown
  • Imaging system for C. elegans behavior / Matthew A. Churgin and Christopher Fang-Yen
  • Method for obtaining large populations of synchronized Caenorhabditis elegans dauer larvae / Maria C. Ow and Sarah E. Hall
  • Sampling and isolation of C. elegans from the natural habitat / Nausicaa Poullet and Christian Braendle
  • Primer on prototyping / Dylan Lynch and David Biron
  • Primer on quantitative modeling / Iulia Neagu and Erel Levine.
Medical Library (Lane)
Status of items at Medical Library (Lane)
Medical Library (Lane) Status
Check Lane Library catalog for status
SPRINGER PROTOCOLS Unknown
Book
online resource (xvi, 472 pages) : illustrations ; 26 cm
  • Classes of cell-penetrating peptides / Margus Pooga and Ülo Langel
  • Penetratin story : an overview / Edmond Dupont, Alain Prochiantz, and Alain Joliot
  • Prediction of cell-penetrating peptides / Mattias Hällbrink and Mati Karelson
  • Computer-aided virtual screening and designing of cell- penetrating peptides / Ankur Gautam, Kumardeep Chaudhary, Rahul Kumar, and Gajendra Pal Singh Raghava
  • Investigating membrane interactions and structures of CPPs / Fatemeh Madani and Astrid Gräslund
  • Determining the effects of membrane-interacting peptides on membrane integrity / William C. Wimley
  • Study of CPP mechanisms by mass spectrometry / Sandrine Sagan, Chérine Bechara, and Fabienne Burlina
  • Methods to study the role of the glycocalyx in the uptake of cell-penetrating peptides / Samuel Schmidt, Rike Wallbrecher, Toin H. van Kuppevelt, and Roland Brock
  • Toxicity, immunogenicity, uptake, and kinetics methods for CPPs / Julia Uusna, Kent Langel, and Ülo Langel
  • Unraveling the mechanisms of peptide-mediated delivery of nucleic acids using electron microscopy / Helerin Margus, Carmen Juks, and Margus Pooga
  • SCARA involvement in the uptake of nanoparticles formed by cell-penetrating peptides / Henrik Helmfors, Staffan Lindberg, and Ülo Langel
  • Protein mimicry and the design of bioactive Cell- penetrating peptides / John Howl and Sarah Jones
  • Pepducins and other lipidated peptides as mechanistic probes and therapeutics / Ping Zhang, Lidija Covic, and Athan Kuliopulos
  • Identification and characterization of homing peptides using in vivo peptide phage display / Maija Hyvönen and Pirjo Laakkonen
  • Antimicrobial and antiviral applications of cell- penetrating peptides / Kalle Pärn, Elo Eriste, and Ülo Langel
  • Visualizing actin architectures in cells incubated with cell-penetrating peptides / Lin He, Peter D. Watson, and Arwyn T. Jones
  • Cell-penetrating peptides as carriers for transepithelial drug delivery in vitro / Stine Rønholt, Mie Kristensen, and Hanne Mørck Nielsen
  • Pathway toward tumor cell-selective CPPs? / Isabel D. Alves, Manon Carré, and Solange Lavielle
  • PepFects and nickFects for the intracellular delivery of nucleic acids / Piret Arukuusk, Ly Pärnaste, Mattias Hällbrink, and Ülo Langel
  • In vitro assays to assess exon skipping in duchenne muscular dystrophy / Prisca Boisguerin, Liz O'Donovan, Michael J. Gait, and Bernard Lebleu
  • Applications of ApoB LDLR-binding domain approach for the development of CNS-penetrating peptides for alzheimer's disease / Eliezer Masliah and Brian Spencer
  • CPP-based delivery system for in vivo gene delivery / Kaido Kurrikoff, Kadi-Liis Veiman, and Ülo Langel
  • Application of CPPs for brain delivery / Artita Srimanee, Jakob Regberg, and Ülo Langel
  • Intracellular delivery of nanoparticles with cell penetrating peptides / Giuseppina Salzano and Vladimir P. Torchilin
  • Multifunctional oligoaminoamides for the receptor-specific delivery of therapeutic RNA / Judith Weber, Ulrich Lächelt, and Ernst Wagner
  • Cell penetrating peptides for chemical biological studies / Ikuhiko Nakase, Toshihide Takeuchi, and Shiroh Futaki
  • Experiences with CPP-based self assembling peptide systems for topical delivery of botulinum toxin / Jane Lee, Phil Kennedy, and Jacob M. Waugh
  • Applications of CPPs in genome modulation of plants / Alicja Ziemienowicz, Jordan Pepper, and François Eudes
  • DNA transfer into animal cells using stearylated CPP based Transfection Reagent / Kristiina Karro, Tiiu Männik, Andres Männik, and Mart Ustav
  • Live cell genomics : cell-specific transcriptome capture in live tissues and cells / Thomas J. Bell and James Eberwine
  • Live cell genomics : RNA exon-specific RNA-binding protein isolation / Thomas J. Bell and James Eberwine.
  • Classes of cell-penetrating peptides / Margus Pooga and Ülo Langel
  • Penetratin story : an overview / Edmond Dupont, Alain Prochiantz, and Alain Joliot
  • Prediction of cell-penetrating peptides / Mattias Hällbrink and Mati Karelson
  • Computer-aided virtual screening and designing of cell- penetrating peptides / Ankur Gautam, Kumardeep Chaudhary, Rahul Kumar, and Gajendra Pal Singh Raghava
  • Investigating membrane interactions and structures of CPPs / Fatemeh Madani and Astrid Gräslund
  • Determining the effects of membrane-interacting peptides on membrane integrity / William C. Wimley
  • Study of CPP mechanisms by mass spectrometry / Sandrine Sagan, Chérine Bechara, and Fabienne Burlina
  • Methods to study the role of the glycocalyx in the uptake of cell-penetrating peptides / Samuel Schmidt, Rike Wallbrecher, Toin H. van Kuppevelt, and Roland Brock
  • Toxicity, immunogenicity, uptake, and kinetics methods for CPPs / Julia Uusna, Kent Langel, and Ülo Langel
  • Unraveling the mechanisms of peptide-mediated delivery of nucleic acids using electron microscopy / Helerin Margus, Carmen Juks, and Margus Pooga
  • SCARA involvement in the uptake of nanoparticles formed by cell-penetrating peptides / Henrik Helmfors, Staffan Lindberg, and Ülo Langel
  • Protein mimicry and the design of bioactive Cell- penetrating peptides / John Howl and Sarah Jones
  • Pepducins and other lipidated peptides as mechanistic probes and therapeutics / Ping Zhang, Lidija Covic, and Athan Kuliopulos
  • Identification and characterization of homing peptides using in vivo peptide phage display / Maija Hyvönen and Pirjo Laakkonen
  • Antimicrobial and antiviral applications of cell- penetrating peptides / Kalle Pärn, Elo Eriste, and Ülo Langel
  • Visualizing actin architectures in cells incubated with cell-penetrating peptides / Lin He, Peter D. Watson, and Arwyn T. Jones
  • Cell-penetrating peptides as carriers for transepithelial drug delivery in vitro / Stine Rønholt, Mie Kristensen, and Hanne Mørck Nielsen
  • Pathway toward tumor cell-selective CPPs? / Isabel D. Alves, Manon Carré, and Solange Lavielle
  • PepFects and nickFects for the intracellular delivery of nucleic acids / Piret Arukuusk, Ly Pärnaste, Mattias Hällbrink, and Ülo Langel
  • In vitro assays to assess exon skipping in duchenne muscular dystrophy / Prisca Boisguerin, Liz O'Donovan, Michael J. Gait, and Bernard Lebleu
  • Applications of ApoB LDLR-binding domain approach for the development of CNS-penetrating peptides for alzheimer's disease / Eliezer Masliah and Brian Spencer
  • CPP-based delivery system for in vivo gene delivery / Kaido Kurrikoff, Kadi-Liis Veiman, and Ülo Langel
  • Application of CPPs for brain delivery / Artita Srimanee, Jakob Regberg, and Ülo Langel
  • Intracellular delivery of nanoparticles with cell penetrating peptides / Giuseppina Salzano and Vladimir P. Torchilin
  • Multifunctional oligoaminoamides for the receptor-specific delivery of therapeutic RNA / Judith Weber, Ulrich Lächelt, and Ernst Wagner
  • Cell penetrating peptides for chemical biological studies / Ikuhiko Nakase, Toshihide Takeuchi, and Shiroh Futaki
  • Experiences with CPP-based self assembling peptide systems for topical delivery of botulinum toxin / Jane Lee, Phil Kennedy, and Jacob M. Waugh
  • Applications of CPPs in genome modulation of plants / Alicja Ziemienowicz, Jordan Pepper, and François Eudes
  • DNA transfer into animal cells using stearylated CPP based Transfection Reagent / Kristiina Karro, Tiiu Männik, Andres Männik, and Mart Ustav
  • Live cell genomics : cell-specific transcriptome capture in live tissues and cells / Thomas J. Bell and James Eberwine
  • Live cell genomics : RNA exon-specific RNA-binding protein isolation / Thomas J. Bell and James Eberwine.
Medical Library (Lane)
Status of items at Medical Library (Lane)
Medical Library (Lane) Status
Check Lane Library catalog for status
SPRINGER PROTOCOLS Unknown
Video
1 online resource (1 streaming video file (55 min.) : color, sound).
  • Contents: Ciliopathies: multisystemic disorders caused by structural and/or functional defects of the primary cilium
  • Mutations in almost 100 genes discovered to date, no clear pattern between specific genes and specific phenotypes
  • Functional interpretation of variants critical for understanding the contribution of alleles to disease severity and pleiotropy
  • A systems-based consideration for the total amount of pathogenic variation in the ciliary proteome begins to predict clinical substructure.
  • Contents: Ciliopathies: multisystemic disorders caused by structural and/or functional defects of the primary cilium
  • Mutations in almost 100 genes discovered to date, no clear pattern between specific genes and specific phenotypes
  • Functional interpretation of variants critical for understanding the contribution of alleles to disease severity and pleiotropy
  • A systems-based consideration for the total amount of pathogenic variation in the ciliary proteome begins to predict clinical substructure.
Book
1 online resource.
Initial proposal summary: The evolution of antibiotic-resistant mutants among bacteria (superbugs) is a persistent and growing threat to public health. In many ways, we are engaged in a war with these microorganisms, where the corresponding arms race involves chemical weapons and biological targets. Just as advances in microelectronics, imaging technology and feature recognition software have turned conventional munitions into smart bombs, the long-term objectives of this proposal are to develop highly effective antibiotics using next-generation biomolecular modeling capabilities in tandem with novel subatomic feature detection software. Using model compounds and targets, our design methodology will be validated with correspondingly ultra-high resolution structure-determination methods at premier DOE facilities (single-crystal X-ray diffraction at Argonne National Laboratory, and neutron diffraction at Oak Ridge National Laboratory). The objectives and accomplishments are summarized.
Initial proposal summary: The evolution of antibiotic-resistant mutants among bacteria (superbugs) is a persistent and growing threat to public health. In many ways, we are engaged in a war with these microorganisms, where the corresponding arms race involves chemical weapons and biological targets. Just as advances in microelectronics, imaging technology and feature recognition software have turned conventional munitions into smart bombs, the long-term objectives of this proposal are to develop highly effective antibiotics using next-generation biomolecular modeling capabilities in tandem with novel subatomic feature detection software. Using model compounds and targets, our design methodology will be validated with correspondingly ultra-high resolution structure-determination methods at premier DOE facilities (single-crystal X-ray diffraction at Argonne National Laboratory, and neutron diffraction at Oak Ridge National Laboratory). The objectives and accomplishments are summarized.
Book
online resource (xiii, 509 pages) : illustrations (some color)
  • Principles of cryopreservation / David E. Pegg
  • Principles of cryopreservation by vitrification / Gregory M. Fahy and Brian Wowk
  • Modeling and optimization of cryopreservation / James D. Benson
  • Principles of freeze-drying / Gerald D. J. Adams, Isobel Cook, and Kevin R. Ward
  • Use of in situ fourier transform infrared spectroscopy to study freezing and drying of cells / Willem F. Wolkers and Harriëtte Oldenhof
  • Calorimetric analysis of cryopreservation and freeze-drying formulations / Wendell Q. Sun
  • Measurement of intracellular ice formation kinetics by high-speed video cryomicroscopy / Jens O. M. Karlsson
  • Laser scanning microscopy in cryobiology / Frank Stracke, Asger Kreiner-Møller, and Heiko Zimmermann
  • Low-temperature electron microscopy : techniques and protocols / Roland A. Fleck
  • Cryopreservation of semen from domestic livestock / Harald Sieme and Harriëtte Oldenhof
  • Cryopreservation of mammalian oocytes / Victoria Keros and Barry J. Fuller
  • Vitrification : a simple and successful method for cryostorage of human blastocysts / Juergen Liebermann
  • Efficient cryopreservation of human pluripotent stem cells by surface-based vitrification / Julia C. Neubauer [and 3 others]
  • Cryopreservation of Greenshell mussel (Perna canaliculus) sperm / Serean L. Adams [and 4 others]
  • Membrane modification strategies for cryopreservation / Phillip H. Purdy and James K. Graham
  • Sperm cleanup and centrifugation processing for cryopreservation / Harald Sieme and Harriëtte Oldenhof
  • Cryopreservation of red blood cells / Johan W. Lagerberg
  • Cord blood clinical processing, cryopreservation, and storage / Heidi Elmoazzen and Jelena L. Holovati
  • Directional freezing for large volume cryopreservation / Joseph Saragusty
  • Vitrification of heart valve tissues / Kelvin G. M. Brockbank [and 3 others]
  • Cryopreservation of plant cell lines / Heinz Martin Schumacher, Martina Westphal, and Elke Heine-Dobbernack
  • Writing standard operating procedures (SOPs) for cryostorage protocols : using shoot meristem cryopreservation as an example / Keith Harding and Erica E. Benson
  • Freeze-drying of proteins / Baolin Liu and Xinli Zhou
  • Freeze-drying of lactic acid Bbacteria / Fernanda Fonseca , Stéphanie Cenard , and Stéphanie Passot
  • Freeze-drying of mammalian sperm / Levent Keskintepe and Ali Eroglu
  • Freeze-drying of decellularized deart valve tissues / Willem F. Wolkers and Andres Hilfiker.
This volume provides a variety of standard protocols used to cryopreserve or freeze-dry different types of specimens. In addition, it provides chapters focused on the fundamental principles of cryopreservation, vitrification, and freeze-drying. Several state of the art microscopic, spectroscopic as well as calorimetric methods are highlighted that can be used to study cellular and macromolecular changes in response to freezing or drying. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cryopreservation and Freeze-Drying Protocols, Third Edition serves as a practical guideline for studies on freezing and drying processes as well as preservation strategies for biological specimens.
  • Principles of cryopreservation / David E. Pegg
  • Principles of cryopreservation by vitrification / Gregory M. Fahy and Brian Wowk
  • Modeling and optimization of cryopreservation / James D. Benson
  • Principles of freeze-drying / Gerald D. J. Adams, Isobel Cook, and Kevin R. Ward
  • Use of in situ fourier transform infrared spectroscopy to study freezing and drying of cells / Willem F. Wolkers and Harriëtte Oldenhof
  • Calorimetric analysis of cryopreservation and freeze-drying formulations / Wendell Q. Sun
  • Measurement of intracellular ice formation kinetics by high-speed video cryomicroscopy / Jens O. M. Karlsson
  • Laser scanning microscopy in cryobiology / Frank Stracke, Asger Kreiner-Møller, and Heiko Zimmermann
  • Low-temperature electron microscopy : techniques and protocols / Roland A. Fleck
  • Cryopreservation of semen from domestic livestock / Harald Sieme and Harriëtte Oldenhof
  • Cryopreservation of mammalian oocytes / Victoria Keros and Barry J. Fuller
  • Vitrification : a simple and successful method for cryostorage of human blastocysts / Juergen Liebermann
  • Efficient cryopreservation of human pluripotent stem cells by surface-based vitrification / Julia C. Neubauer [and 3 others]
  • Cryopreservation of Greenshell mussel (Perna canaliculus) sperm / Serean L. Adams [and 4 others]
  • Membrane modification strategies for cryopreservation / Phillip H. Purdy and James K. Graham
  • Sperm cleanup and centrifugation processing for cryopreservation / Harald Sieme and Harriëtte Oldenhof
  • Cryopreservation of red blood cells / Johan W. Lagerberg
  • Cord blood clinical processing, cryopreservation, and storage / Heidi Elmoazzen and Jelena L. Holovati
  • Directional freezing for large volume cryopreservation / Joseph Saragusty
  • Vitrification of heart valve tissues / Kelvin G. M. Brockbank [and 3 others]
  • Cryopreservation of plant cell lines / Heinz Martin Schumacher, Martina Westphal, and Elke Heine-Dobbernack
  • Writing standard operating procedures (SOPs) for cryostorage protocols : using shoot meristem cryopreservation as an example / Keith Harding and Erica E. Benson
  • Freeze-drying of proteins / Baolin Liu and Xinli Zhou
  • Freeze-drying of lactic acid Bbacteria / Fernanda Fonseca , Stéphanie Cenard , and Stéphanie Passot
  • Freeze-drying of mammalian sperm / Levent Keskintepe and Ali Eroglu
  • Freeze-drying of decellularized deart valve tissues / Willem F. Wolkers and Andres Hilfiker.
This volume provides a variety of standard protocols used to cryopreserve or freeze-dry different types of specimens. In addition, it provides chapters focused on the fundamental principles of cryopreservation, vitrification, and freeze-drying. Several state of the art microscopic, spectroscopic as well as calorimetric methods are highlighted that can be used to study cellular and macromolecular changes in response to freezing or drying. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cryopreservation and Freeze-Drying Protocols, Third Edition serves as a practical guideline for studies on freezing and drying processes as well as preservation strategies for biological specimens.
Medical Library (Lane)
Status of items at Medical Library (Lane)
Medical Library (Lane) Status
Check Lane Library catalog for status
SPRINGER PROTOCOLS Unknown
Book
1 online resource (e0121177 ) : digital, PDF file.
More than 30% of acute myeloid leukemia (AML) patients possess activating mutations in the receptor tyrosine kinase FMS-like tyrosine kinase 3 or FLT3. A small-molecule inhibitor of FLT3 (known as quizartinib or AC220) that is currently in clinical trials appears promising for the treatment of AML. Here, we report the co-crystal structure of the kinase domain of FLT3 in complex with quizartinib. FLT3 with quizartinib bound adopts an “Abl-like” inactive conformation with the activation loop stabilized in the “DFG-out” orientation and folded back onto the kinase domain. This conformation is similar to that observed for the uncomplexed intracellular domain of FLT3 as well as for related receptor tyrosine kinases, except for a localized induced fit in the activation loop. The co-crystal structure reveals the interactions between quizartinib and the active site of FLT3 that are key for achieving its high potency against both wild-type FLT3 as well as a FLT3 variant observed in many AML patients. This co-complex further provides a structural rationale for quizartinib-resistance mutations.
More than 30% of acute myeloid leukemia (AML) patients possess activating mutations in the receptor tyrosine kinase FMS-like tyrosine kinase 3 or FLT3. A small-molecule inhibitor of FLT3 (known as quizartinib or AC220) that is currently in clinical trials appears promising for the treatment of AML. Here, we report the co-crystal structure of the kinase domain of FLT3 in complex with quizartinib. FLT3 with quizartinib bound adopts an “Abl-like” inactive conformation with the activation loop stabilized in the “DFG-out” orientation and folded back onto the kinase domain. This conformation is similar to that observed for the uncomplexed intracellular domain of FLT3 as well as for related receptor tyrosine kinases, except for a localized induced fit in the activation loop. The co-crystal structure reveals the interactions between quizartinib and the active site of FLT3 that are key for achieving its high potency against both wild-type FLT3 as well as a FLT3 variant observed in many AML patients. This co-complex further provides a structural rationale for quizartinib-resistance mutations.
Video
1 online resource (1 streaming video file (35 min.) : color, sound).
  • Contents: Introduction to genetic drift
  • Hardy-Weinberg equilibrium
  • Experimental observations of genetic drift
  • Wright-Fisher model
  • Computational simulations.
  • Contents: Introduction to genetic drift
  • Hardy-Weinberg equilibrium
  • Experimental observations of genetic drift
  • Wright-Fisher model
  • Computational simulations.
Video
1 online resource (1 streaming video file (28 min.) : color, sound).
  • Contents: Quantifying human skeletal variation
  • Genetic & environmental basis of human skeletal variation
  • Observations about human genetic variation
  • Patterns of cranial variation vs. patterns of genetic variation
  • Evolutionary processes that shaped patterns of cranial variation
  • Humans in comparison with other taxa.
  • Contents: Quantifying human skeletal variation
  • Genetic & environmental basis of human skeletal variation
  • Observations about human genetic variation
  • Patterns of cranial variation vs. patterns of genetic variation
  • Evolutionary processes that shaped patterns of cranial variation
  • Humans in comparison with other taxa.
Book
1 online resource. Digital: text file; PDF.
  • Introduction.- Introduction and prospects of Marine natural compounds.- Development of Anticancer Drugs from Marine Sources.- Seaweeds.- Bacteria and Cyanobacteria Fungal metabolites.- Sponge derived bioactive compounds Mollusk.- Soft corals.- Algae.- Tunicate.- Other marine organisms derived compounds.
  • (source: Nielsen Book Data)
This timely desk reference focuses on marine-derived bioactive substances which have biological, medical and industrial applications. The medicinal value of these marine natural products are assessed and discussed. Their function as a new and important resource in novel, anticancer drug discovery research is also presented in international contributions from several research groups. For example, the potential role of Spongistatin, Apratoxin A, Eribulin mesylate, phlorotannins, fucoidan, as anticancer agents is explained. The mechanism of action of bioactive compounds present in marine algae, bacteria, fungus, sponges, seaweeds and other marine animals and plants are illustrated via several mechanisms. In addition, this handbook lists various compounds that are active candidates in chemoprevention and their target actions. The handbook also places into context the demand for anticancer nutraceuticals and their use as potential anti-cancer pharmaceuticals and medicines. This study of advanced and future types of natural compounds from marine sources is written to facilitate the understanding of Biotechnology and its application to marine natural product drug discovery research.
(source: Nielsen Book Data)
  • Introduction.- Introduction and prospects of Marine natural compounds.- Development of Anticancer Drugs from Marine Sources.- Seaweeds.- Bacteria and Cyanobacteria Fungal metabolites.- Sponge derived bioactive compounds Mollusk.- Soft corals.- Algae.- Tunicate.- Other marine organisms derived compounds.
  • (source: Nielsen Book Data)
This timely desk reference focuses on marine-derived bioactive substances which have biological, medical and industrial applications. The medicinal value of these marine natural products are assessed and discussed. Their function as a new and important resource in novel, anticancer drug discovery research is also presented in international contributions from several research groups. For example, the potential role of Spongistatin, Apratoxin A, Eribulin mesylate, phlorotannins, fucoidan, as anticancer agents is explained. The mechanism of action of bioactive compounds present in marine algae, bacteria, fungus, sponges, seaweeds and other marine animals and plants are illustrated via several mechanisms. In addition, this handbook lists various compounds that are active candidates in chemoprevention and their target actions. The handbook also places into context the demand for anticancer nutraceuticals and their use as potential anti-cancer pharmaceuticals and medicines. This study of advanced and future types of natural compounds from marine sources is written to facilitate the understanding of Biotechnology and its application to marine natural product drug discovery research.
(source: Nielsen Book Data)
Video
1 online resource (1 streaming video file (37 min.) : color, sound).
  • Contents: Genetic admixture processes
  • Statistical approach to allele frequencies in admixed populations
  • General mechanistic approach to complex admixture processes
  • Human admixture: isolation, migration and sociocultural behavior
  • Genetic admixture in the evolutionary history of Homo sapiens.
  • Contents: Genetic admixture processes
  • Statistical approach to allele frequencies in admixed populations
  • General mechanistic approach to complex admixture processes
  • Human admixture: isolation, migration and sociocultural behavior
  • Genetic admixture in the evolutionary history of Homo sapiens.
Video
1 online resource (1 streaming video file (24 min.) : color, sound).
  • Contents: Human-chimpanzee similarities
  • Human-chimpanzee genomic comparison
  • DNA duplication in human evolution
  • DNA substitution in human evolution
  • Modeling molecular evolution
  • 2 goals of comparative genomics
  • Identifying human accelerated regions (HARs).
  • Contents: Human-chimpanzee similarities
  • Human-chimpanzee genomic comparison
  • DNA duplication in human evolution
  • DNA substitution in human evolution
  • Modeling molecular evolution
  • 2 goals of comparative genomics
  • Identifying human accelerated regions (HARs).
Book
online resource (xx, 592 pages) : illustrations ; 26 cm
  • Optimization of in situ hybridization and co-expression analysis in the central nervous system / Louisa Mezache ... [et al.]
  • In situ hybridization methods for RNA visualization in C. elegans / Gina Broitman-Maduro and Morris F. Maduro
  • Multicolored visualization of transcript distributions in Drosophila embryos / Iris Söll and Giselbert Hauptmann
  • Exploring brain genoarchitecture by single and double chromogenic in situ hybridization (ISH) and immunohistochemistry (IHC) in whole-mount embryos / José Luis ... [et al.]
  • Exploring brain genoarchitecture by single and double chromogenic in situ hybridization (ISH) and immunohistochemistry (IHC) on cryostat, paraffin, or floating sections / José Luis Ferran ... [et al.]
  • Combined radioactive and nonradioactive double in situ hybridization (DISH) / Jong-Hyun Son and Ursula H. Winzer-Serhan
  • One FISH, dFISH, three FISH : sensitive methods of whole- mount fluorescent in situ hybridization in freshwater planarians / David D. R. Brown and Bret J. Pearson
  • Whole-mount in situ hybridization and immunohistochemistry in xenopus embryos / Edgar M. Pera ... [et al.]
  • Manual and automated whole-mount in situ hybridization for systematic gene expression analysis in embryonic Zebrafish forebrain / Iris Söll and Giselbert Hauptmann
  • Large-scale ISH on mouse brain sections for systematic gene expression analysis in developing mouse diencephalon / Tomomi Shimogori and Seth Blackshaw
  • High-throughput in situ hybridization : systematical production of gene expression data and beyond / Lars Geffers and Gregor Eichele
  • Analysis of embryonic gene expression patterns in the hagfish / Yasuhiro Oisi ... [et al.]
  • Gene expression analysis of lamprey embryos / Fumiaki Sugahara, Yasunori Murakami, and Shigeru Kuratani
  • Detection of mRNA and microRNA expression in basal chordates, amphioxus and ascidians / Simona Candiani, Greta Garbarino, and Mario Pestarino
  • Analysis of gene expression in neurons and synapses by multi-color in situ hybridization / Leonid L. Moroz and Andrea B. Kohn
  • Fluorescent in situ hybridization in primary hippocampal neurons to detect localized mRNAs / Jacki E. Heraud-Farlow, Tejaswini Sharangdhar, and Michael A. Kiebler
  • Combining whole-mount in situ hybridization with neuronal tracing and immunohistochemistry / Jeremy S. Duncan ... [et al.]
  • Fluorescent in situ hybridization detection of microRNAs in Newt tissue cryosections / Jamie Ian Morrison
  • Subcellular transcript localization in Drosophila embryos and tissues visualized by multiplex-FISH / Julie Bergalet ... [et al.]
  • Application of alkaline phosphatase-mediated azo dye staining for dual fluorescent in situ hybridization in Zebrafish / Giselbert Hauptmann, Gilbert Lauter, and Iris Söll
  • Multiplex fluorescent RNA in situ hybridization via RNAscope / Hongwei Wang ... [et al.]
  • Quantum dot-based in situ hybridization and immunohistochemistry to detect mRNA and protein at subcellular levels, comparison with studies using electron microscopy / Akira Matsuno ... [et al.]
  • Quantitative multiplexed quantum dot based in situ hybridization in formalin-fixed paraffin-embedded tissue / Eleni Tholouli, Judith A. Hoyland, and Richard J. Byers
  • Single and multiplexed fluorescent in situ hybridization using quantum dots nanocrystals in xenopus / Andriani Ioannou ... [et al.]
  • Quantitative single-molecule mRNA fluorescent in situ hybridization in C. elegans / Remco A. Mentink ... [et al.]
  • In situ detection of individual mRNA molecules with padlock probes and target-primed rolling-circle amplification in fixed mouse brain tissues / Thomas Hauling ... [et al.]
  • Extended multiplexed fluorescent in situ hybridization by combinatorial encoding of individual transcripts / Lars Martin Jakt and Satoko Moriwaki
  • Characterizing cellular identity at one cell resolution / Amaresh K. Ranjan, Mugdha V. Joglekar, and Anandwardhan A. Hardikar
  • Transglutaminase-mediated in situ hybridization (TransISH) for mRNA detection in mammalian tissues / Katsuyuki Miyawaki, Sumihare Noji, and Noriho Kamiya
  • ECHO-FISH for gene transcript detection in neuronal and other cells and subcellular compartments / Dan Ohtan Wang and Akimitsu Okamoto.
  • Optimization of in situ hybridization and co-expression analysis in the central nervous system / Louisa Mezache ... [et al.]
  • In situ hybridization methods for RNA visualization in C. elegans / Gina Broitman-Maduro and Morris F. Maduro
  • Multicolored visualization of transcript distributions in Drosophila embryos / Iris Söll and Giselbert Hauptmann
  • Exploring brain genoarchitecture by single and double chromogenic in situ hybridization (ISH) and immunohistochemistry (IHC) in whole-mount embryos / José Luis ... [et al.]
  • Exploring brain genoarchitecture by single and double chromogenic in situ hybridization (ISH) and immunohistochemistry (IHC) on cryostat, paraffin, or floating sections / José Luis Ferran ... [et al.]
  • Combined radioactive and nonradioactive double in situ hybridization (DISH) / Jong-Hyun Son and Ursula H. Winzer-Serhan
  • One FISH, dFISH, three FISH : sensitive methods of whole- mount fluorescent in situ hybridization in freshwater planarians / David D. R. Brown and Bret J. Pearson
  • Whole-mount in situ hybridization and immunohistochemistry in xenopus embryos / Edgar M. Pera ... [et al.]
  • Manual and automated whole-mount in situ hybridization for systematic gene expression analysis in embryonic Zebrafish forebrain / Iris Söll and Giselbert Hauptmann
  • Large-scale ISH on mouse brain sections for systematic gene expression analysis in developing mouse diencephalon / Tomomi Shimogori and Seth Blackshaw
  • High-throughput in situ hybridization : systematical production of gene expression data and beyond / Lars Geffers and Gregor Eichele
  • Analysis of embryonic gene expression patterns in the hagfish / Yasuhiro Oisi ... [et al.]
  • Gene expression analysis of lamprey embryos / Fumiaki Sugahara, Yasunori Murakami, and Shigeru Kuratani
  • Detection of mRNA and microRNA expression in basal chordates, amphioxus and ascidians / Simona Candiani, Greta Garbarino, and Mario Pestarino
  • Analysis of gene expression in neurons and synapses by multi-color in situ hybridization / Leonid L. Moroz and Andrea B. Kohn
  • Fluorescent in situ hybridization in primary hippocampal neurons to detect localized mRNAs / Jacki E. Heraud-Farlow, Tejaswini Sharangdhar, and Michael A. Kiebler
  • Combining whole-mount in situ hybridization with neuronal tracing and immunohistochemistry / Jeremy S. Duncan ... [et al.]
  • Fluorescent in situ hybridization detection of microRNAs in Newt tissue cryosections / Jamie Ian Morrison
  • Subcellular transcript localization in Drosophila embryos and tissues visualized by multiplex-FISH / Julie Bergalet ... [et al.]
  • Application of alkaline phosphatase-mediated azo dye staining for dual fluorescent in situ hybridization in Zebrafish / Giselbert Hauptmann, Gilbert Lauter, and Iris Söll
  • Multiplex fluorescent RNA in situ hybridization via RNAscope / Hongwei Wang ... [et al.]
  • Quantum dot-based in situ hybridization and immunohistochemistry to detect mRNA and protein at subcellular levels, comparison with studies using electron microscopy / Akira Matsuno ... [et al.]
  • Quantitative multiplexed quantum dot based in situ hybridization in formalin-fixed paraffin-embedded tissue / Eleni Tholouli, Judith A. Hoyland, and Richard J. Byers
  • Single and multiplexed fluorescent in situ hybridization using quantum dots nanocrystals in xenopus / Andriani Ioannou ... [et al.]
  • Quantitative single-molecule mRNA fluorescent in situ hybridization in C. elegans / Remco A. Mentink ... [et al.]
  • In situ detection of individual mRNA molecules with padlock probes and target-primed rolling-circle amplification in fixed mouse brain tissues / Thomas Hauling ... [et al.]
  • Extended multiplexed fluorescent in situ hybridization by combinatorial encoding of individual transcripts / Lars Martin Jakt and Satoko Moriwaki
  • Characterizing cellular identity at one cell resolution / Amaresh K. Ranjan, Mugdha V. Joglekar, and Anandwardhan A. Hardikar
  • Transglutaminase-mediated in situ hybridization (TransISH) for mRNA detection in mammalian tissues / Katsuyuki Miyawaki, Sumihare Noji, and Noriho Kamiya
  • ECHO-FISH for gene transcript detection in neuronal and other cells and subcellular compartments / Dan Ohtan Wang and Akimitsu Okamoto.
Medical Library (Lane)
Status of items at Medical Library (Lane)
Medical Library (Lane) Status
Check Lane Library catalog for status
SPRINGER PROTOCOLS Unknown
Book
online resource (xi, 327 pages) : illustrations
  • Introduction to membrane lipids /Richard M. Epand
  • Introduction : membrane properties (good) for life /Marek Cebecauer
  • Introduction to fluorescence probing of biological membranes /Alexander P. Demchenko ... [et al.]
  • Assembly and use of tethered bilayer lipid membranes (tBLMs) /Charles Cranfield ... [et al.]
  • Preparation of detergent-resistant membranes (DRMs) from cultured mammalian cells /Deborah A. Brown
  • Isolation of giant plasma membrane vesicles for evaluation of plasma membrane structure and protein partitioning /K. R. Levental and I. Levental
  • Asymmetric giant lipid vesicle fabrication /Peichi C. Hu and Noah Malmstadt
  • Cholesterol depletion using Methyl-[Beta]-cyclodextrin /Saleemulla Mahammad and Ingela Parmryd
  • Comparative LC-MS based profiling approach to analyze lipid composition in tissue culture systems /G. Ekin Atilla-Gokcumen and Ulrike S. Eggert
  • Imaging membrane order using environmentally sensitive fluorophores /G.W. Ashdown and Dylan M. Owen
  • 3D super-resolution imaging by localization microscopy /Astrid Magenau and Katharina Gaus
  • Electron microscopy methods for studying plasma membranes /Alison J. Beckett and Ian A. Prior
  • Measuring cytoskeleton and cellular membrane mechanical properties by atomic force microscopy /Charles Roduit ... [et al.]
  • Fluorescence linear dichroism imaging for quantifying membrane order /Richard K. P. Benninger
  • Scanning fluorescence correlation spectroscopy on biomembranes /Eduard Hermann, Jonas Ries, and Ana J. García-Sáez
  • X-ray diffraction of lipid model membranes /Arwen I. I. Tyler, Robert V. Law, and John M. Seddon
  • Solid state NMR of lipid model membranes /Arwen I. I. Tyler ... [et al.]
  • Fluorescence recovery after photobleaching (FRAP) : acquisition, analysis, and applications /Michael Carnell, Alex Macmillan, and Renee Whan
  • Laurdan spectral phasor method to explore membrane micro-heterogeneity and lipid domains in live cells /Ottavia Golfetto, Elizabeth Hinde, and Enrico Gratton
  • Cholesterol behavior in asymmetric lipid bilayers : insights from molecular dynamics simulations /Semen O. Yesylevskyy and Alexander P. Demchenko
  • Computer simulations of phase separation in lipid bilayers and monolayers /Svetlana Baoukina and D. Peter Tieleman.
  • Introduction to membrane lipids /Richard M. Epand
  • Introduction : membrane properties (good) for life /Marek Cebecauer
  • Introduction to fluorescence probing of biological membranes /Alexander P. Demchenko ... [et al.]
  • Assembly and use of tethered bilayer lipid membranes (tBLMs) /Charles Cranfield ... [et al.]
  • Preparation of detergent-resistant membranes (DRMs) from cultured mammalian cells /Deborah A. Brown
  • Isolation of giant plasma membrane vesicles for evaluation of plasma membrane structure and protein partitioning /K. R. Levental and I. Levental
  • Asymmetric giant lipid vesicle fabrication /Peichi C. Hu and Noah Malmstadt
  • Cholesterol depletion using Methyl-[Beta]-cyclodextrin /Saleemulla Mahammad and Ingela Parmryd
  • Comparative LC-MS based profiling approach to analyze lipid composition in tissue culture systems /G. Ekin Atilla-Gokcumen and Ulrike S. Eggert
  • Imaging membrane order using environmentally sensitive fluorophores /G.W. Ashdown and Dylan M. Owen
  • 3D super-resolution imaging by localization microscopy /Astrid Magenau and Katharina Gaus
  • Electron microscopy methods for studying plasma membranes /Alison J. Beckett and Ian A. Prior
  • Measuring cytoskeleton and cellular membrane mechanical properties by atomic force microscopy /Charles Roduit ... [et al.]
  • Fluorescence linear dichroism imaging for quantifying membrane order /Richard K. P. Benninger
  • Scanning fluorescence correlation spectroscopy on biomembranes /Eduard Hermann, Jonas Ries, and Ana J. García-Sáez
  • X-ray diffraction of lipid model membranes /Arwen I. I. Tyler, Robert V. Law, and John M. Seddon
  • Solid state NMR of lipid model membranes /Arwen I. I. Tyler ... [et al.]
  • Fluorescence recovery after photobleaching (FRAP) : acquisition, analysis, and applications /Michael Carnell, Alex Macmillan, and Renee Whan
  • Laurdan spectral phasor method to explore membrane micro-heterogeneity and lipid domains in live cells /Ottavia Golfetto, Elizabeth Hinde, and Enrico Gratton
  • Cholesterol behavior in asymmetric lipid bilayers : insights from molecular dynamics simulations /Semen O. Yesylevskyy and Alexander P. Demchenko
  • Computer simulations of phase separation in lipid bilayers and monolayers /Svetlana Baoukina and D. Peter Tieleman.
Medical Library (Lane)
Status of items at Medical Library (Lane)
Medical Library (Lane) Status
Check Lane Library catalog for status
SPRINGER PROTOCOLS Unknown
Book
1 online resource (p. 19-27 ) : digital, PDF file.
We use Molecular Dynamics and Monte Carlo simulations to examine molecular transport phenomena in nanochannels, explaining four orders of magnitude difference in wheat germ agglutinin (WGA) protein diffusion rates observed by fluorescence correlation spectroscopy (FCS) and by direct imaging of fluorescently-labeled proteins. We first use the ESPResSo Molecular Dynamics code to estimate the surface transport distance for neutral and charged proteins. We then employ a Monte Carlo model to calculate the paths of protein molecules on surfaces and in the bulk liquid transport medium. Our results show that the transport characteristics depend strongly on the degree of molecular surface coverage. Atomic force microscope characterization of surfaces exposed to WGA proteins for 1000 s show large protein aggregates consistent with the predicted coverage. These calculations and experiments provide useful insight into the details of molecular motion in confined geometries.
We use Molecular Dynamics and Monte Carlo simulations to examine molecular transport phenomena in nanochannels, explaining four orders of magnitude difference in wheat germ agglutinin (WGA) protein diffusion rates observed by fluorescence correlation spectroscopy (FCS) and by direct imaging of fluorescently-labeled proteins. We first use the ESPResSo Molecular Dynamics code to estimate the surface transport distance for neutral and charged proteins. We then employ a Monte Carlo model to calculate the paths of protein molecules on surfaces and in the bulk liquid transport medium. Our results show that the transport characteristics depend strongly on the degree of molecular surface coverage. Atomic force microscope characterization of surfaces exposed to WGA proteins for 1000 s show large protein aggregates consistent with the predicted coverage. These calculations and experiments provide useful insight into the details of molecular motion in confined geometries.
Book
1 online resource.
  • List of Contributors ix Preface xiii 1 Technological Advances in Studies of Plant Adaptation 1 Jose G. Vallarino and Sonia Osorio Introduction 1 Next-Generation Sequencing Technologies 2 Applications of Next-Generation Sequencing 7 Proteome Analysis in Understanding Plant Adaptation 12 Applications of Proteomics 16 Metabolome Analysis in Plant Adaptation 17 Applications of Metabolic Profiling 18 Concluding Remarks and Future Prospects 21 Acknowledgments 22 References 22 2 Use of Natural Variation in Arabidopsis thaliana to Study Adaptation 31 Lisa M. Smith and Roosa A. E. Laitinen Introduction 31 Genetic Natural Variation 33 Epigenetic Natural Variation 37 Natural Variation and Metabolites 42 Use of A. thaliana Hybrids in Understanding Evolution 46 Conclusion 49 Acknowledgments 50 References 50 3 Seed Dormancy, Longevity and Their Adaptation 61 Thu-Phuong Nguyen and Leonie Bentsink Introduction 61 The Induction of Seed Dormancy and Seed Longevity 62 Factors Affecting Seed Dormancy and Seed Longevity 63 Seed Dry Storage 64 Genetics of Seed Dormancy and Seed Longevity 67 The Relation Between Seed Dormancy and Seed Longevity and its Ecological Significance 70 Ecological Role 70 The Trade-off Between Seed Dormancy and Seed Longevity 73 Conclusions 74 References 74 4 The Gatekeeper Concept: Cell-Type Specific Molecular Mechanisms of Plant Adaptation to Abiotic Stress 83 SamW. Henderson and Matthew Gilliham Introduction 83 The Gatekeeper Concept 85 Single Cell TypesWithin Plant Roots 86 Root Hairs Tolerance to Phosphorus Deficiency 88 Epidermal Cells of the Root Apex Aluminum Tolerance 91 Xylem Parenchyma Cells Salinity Tolerance 94 Pericycle Cells Nitrogen Starvation 99 Endodermal Cells ABA Signaling Under Abiotic Stress 102 Beyond Gatekeepers Conclusions and Perspectives 103 References 105 5 Regulatory and Biosynthetic Mechanisms Underlying Plant Chemical Defense Responses to Biotic Stresses 117 William R. Chezem and Nicole K. Clay Introduction 117 Defensive Phenylpropanoids 119 Defense-Related Regulators of Phenylpropanoid Metabolism 124 Defensive Aromatic Alkaloids 126 Defense-Related Regulators of Aromatic Alkaloid Metabolism 131 Conclusion 134 References 135 6 Role of Small RNAs in Regulation of Plant Responses to Stress 147 Luis A.A. Toledo-Filho and Sascha Laubinger Introduction 147 miRNAs Biogenesis and Function 148 Evolution of miRNAs 149 siRNAs Biogenesis and Function 150 sRNA Stress Responses 151 sRNA in Abiotic Stress Responses 157 Conclusions and Future Prospects 162 References 163 7 Adaptation of Flower Form: An Evo-Devo Approach to Study Adaptive Evolution in Flower Morphology 169 Roxana Yockteng, Ana M.R. Almeida, Alma Pi neyro-Nelson, and Chelsea D. Specht Introduction 169 Flower Developmental Genetics: (A)BCs and Beyond 171 Approaches to the Study of Evolution of Floral Morphology 172 Using GRNs to Investigate Adaptive Evolution of Floral Form: SEP3 as a Case Study 176 Conclusions 184 Acknowledgments 185 References 185 8 Computational Approaches to Dissect and Understand Mechanisms of Adaptation 191 Sabrina Kleessen and Zoran Nikoloski Introduction 191 Experimental Set-Ups for Data Acquisition to Reveal Trade-Offs via Correlations 193 Pareto Front Approaches 195 The Triangulation Criterion 195 Ranking of Genotypes 197 From Models to Elements Contributing to Adaptation 199 Cellular Tasks Involved in Adaptation 202 Minimal Network Adjustments Upon Perturbations 202 Investigation of Network Adjustments by Integrating High-Throughput Data 204 Non-Steady State Behavior and Metabolic Network Adjustments 205 Future Challenges and Perspectives 207 References 208 9 From the Greenhouse to the Real World Arabidopsis Field Trials and Applications 215 Karin Kohl and Roosa A.E. Laitinen Introduction 215 Field Experiments in A. thaliana 216 How to do Field Trials? 220 From Arabidopsis to Crops 228 Future Prospects 230 References 230 Index 235.
  • (source: Nielsen Book Data)
Plants are forced to adapt for a variety of reasons protection, reproductive viability, and environmental and climatic changes. Computational tools and molecular advances have provided researchers with significant new insights into the molecular basis of plant adaptation. Molecular Mechanisms in Plant Adaptation provides a comprehensive overview of a wide variety of these different mechanisms underlying adaptation to these challenges to plant survival. Molecular Mechanisms in Plant Adaptation opens with a chapter that explores the latest technological advances used in plant adaptation research, providing readers with an overview of high-throughput technologies and their applications. The chapters that follow cover the latest developments on using natural variation to dissect genetic, epigenetic and metabolic responses of plant adaptation. Subsequent chapters describe plant responses to biotic and abiotic stressors and adaptive reproductive strategies. Emerging topics such as secondary metabolism, small RNA mediated regulation as well as cell type specific responses to stresses are given special precedence. The book ends with chapters introducing computational approaches to study adaptation and focusing on how to apply laboratory findings to field studies and breeding programs. Molecular Mechanisms in Plant Adaptation interest plant molecular biologists and physiologists, plant stress biologists, plant geneticists and advanced plant biology students.
(source: Nielsen Book Data)
  • List of Contributors ix Preface xiii 1 Technological Advances in Studies of Plant Adaptation 1 Jose G. Vallarino and Sonia Osorio Introduction 1 Next-Generation Sequencing Technologies 2 Applications of Next-Generation Sequencing 7 Proteome Analysis in Understanding Plant Adaptation 12 Applications of Proteomics 16 Metabolome Analysis in Plant Adaptation 17 Applications of Metabolic Profiling 18 Concluding Remarks and Future Prospects 21 Acknowledgments 22 References 22 2 Use of Natural Variation in Arabidopsis thaliana to Study Adaptation 31 Lisa M. Smith and Roosa A. E. Laitinen Introduction 31 Genetic Natural Variation 33 Epigenetic Natural Variation 37 Natural Variation and Metabolites 42 Use of A. thaliana Hybrids in Understanding Evolution 46 Conclusion 49 Acknowledgments 50 References 50 3 Seed Dormancy, Longevity and Their Adaptation 61 Thu-Phuong Nguyen and Leonie Bentsink Introduction 61 The Induction of Seed Dormancy and Seed Longevity 62 Factors Affecting Seed Dormancy and Seed Longevity 63 Seed Dry Storage 64 Genetics of Seed Dormancy and Seed Longevity 67 The Relation Between Seed Dormancy and Seed Longevity and its Ecological Significance 70 Ecological Role 70 The Trade-off Between Seed Dormancy and Seed Longevity 73 Conclusions 74 References 74 4 The Gatekeeper Concept: Cell-Type Specific Molecular Mechanisms of Plant Adaptation to Abiotic Stress 83 SamW. Henderson and Matthew Gilliham Introduction 83 The Gatekeeper Concept 85 Single Cell TypesWithin Plant Roots 86 Root Hairs Tolerance to Phosphorus Deficiency 88 Epidermal Cells of the Root Apex Aluminum Tolerance 91 Xylem Parenchyma Cells Salinity Tolerance 94 Pericycle Cells Nitrogen Starvation 99 Endodermal Cells ABA Signaling Under Abiotic Stress 102 Beyond Gatekeepers Conclusions and Perspectives 103 References 105 5 Regulatory and Biosynthetic Mechanisms Underlying Plant Chemical Defense Responses to Biotic Stresses 117 William R. Chezem and Nicole K. Clay Introduction 117 Defensive Phenylpropanoids 119 Defense-Related Regulators of Phenylpropanoid Metabolism 124 Defensive Aromatic Alkaloids 126 Defense-Related Regulators of Aromatic Alkaloid Metabolism 131 Conclusion 134 References 135 6 Role of Small RNAs in Regulation of Plant Responses to Stress 147 Luis A.A. Toledo-Filho and Sascha Laubinger Introduction 147 miRNAs Biogenesis and Function 148 Evolution of miRNAs 149 siRNAs Biogenesis and Function 150 sRNA Stress Responses 151 sRNA in Abiotic Stress Responses 157 Conclusions and Future Prospects 162 References 163 7 Adaptation of Flower Form: An Evo-Devo Approach to Study Adaptive Evolution in Flower Morphology 169 Roxana Yockteng, Ana M.R. Almeida, Alma Pi neyro-Nelson, and Chelsea D. Specht Introduction 169 Flower Developmental Genetics: (A)BCs and Beyond 171 Approaches to the Study of Evolution of Floral Morphology 172 Using GRNs to Investigate Adaptive Evolution of Floral Form: SEP3 as a Case Study 176 Conclusions 184 Acknowledgments 185 References 185 8 Computational Approaches to Dissect and Understand Mechanisms of Adaptation 191 Sabrina Kleessen and Zoran Nikoloski Introduction 191 Experimental Set-Ups for Data Acquisition to Reveal Trade-Offs via Correlations 193 Pareto Front Approaches 195 The Triangulation Criterion 195 Ranking of Genotypes 197 From Models to Elements Contributing to Adaptation 199 Cellular Tasks Involved in Adaptation 202 Minimal Network Adjustments Upon Perturbations 202 Investigation of Network Adjustments by Integrating High-Throughput Data 204 Non-Steady State Behavior and Metabolic Network Adjustments 205 Future Challenges and Perspectives 207 References 208 9 From the Greenhouse to the Real World Arabidopsis Field Trials and Applications 215 Karin Kohl and Roosa A.E. Laitinen Introduction 215 Field Experiments in A. thaliana 216 How to do Field Trials? 220 From Arabidopsis to Crops 228 Future Prospects 230 References 230 Index 235.
  • (source: Nielsen Book Data)
Plants are forced to adapt for a variety of reasons protection, reproductive viability, and environmental and climatic changes. Computational tools and molecular advances have provided researchers with significant new insights into the molecular basis of plant adaptation. Molecular Mechanisms in Plant Adaptation provides a comprehensive overview of a wide variety of these different mechanisms underlying adaptation to these challenges to plant survival. Molecular Mechanisms in Plant Adaptation opens with a chapter that explores the latest technological advances used in plant adaptation research, providing readers with an overview of high-throughput technologies and their applications. The chapters that follow cover the latest developments on using natural variation to dissect genetic, epigenetic and metabolic responses of plant adaptation. Subsequent chapters describe plant responses to biotic and abiotic stressors and adaptive reproductive strategies. Emerging topics such as secondary metabolism, small RNA mediated regulation as well as cell type specific responses to stresses are given special precedence. The book ends with chapters introducing computational approaches to study adaptation and focusing on how to apply laboratory findings to field studies and breeding programs. Molecular Mechanisms in Plant Adaptation interest plant molecular biologists and physiologists, plant stress biologists, plant geneticists and advanced plant biology students.
(source: Nielsen Book Data)
Book
1 online resource (xiv, 147 pages) : illustrations
  • Taxonomy in the Electronic Age.- Correct Names for Some of the Closest Relatives of Carica Papaya.- A Dated Phylogeny of the Papaya Family.- Chromosome Counts for the Caricaceae Reveal.
  • (source: Nielsen Book Data)
Fernanda Antunes Carvalho addresses an issue of key importance to the field of systematics, namely how to foster taxonomic work and the dissemination of scientific knowledge about species by taking full advantage of electronic data and bioinformatics tools. The first part focuses on the development of an electronic monograph of the papaya family (Caricaceae) using existing infrastructures of Information Technology (IT) and bioinformatic tools that together set the stage for a new era of systematics. Based on the plastid and nuclear DNA data, the author inferred historical processes in the second part that may have shaped the evolution of the Caricaceae and explain their current geographic distribution. The last part is dedicated to the evolution of chromosome numbers in the Caricaceae and includes counts for species from three genera (Cylicomorpha, Horovitzia, Jarilla) that have never been investigated before.
(source: Nielsen Book Data)
  • Taxonomy in the Electronic Age.- Correct Names for Some of the Closest Relatives of Carica Papaya.- A Dated Phylogeny of the Papaya Family.- Chromosome Counts for the Caricaceae Reveal.
  • (source: Nielsen Book Data)
Fernanda Antunes Carvalho addresses an issue of key importance to the field of systematics, namely how to foster taxonomic work and the dissemination of scientific knowledge about species by taking full advantage of electronic data and bioinformatics tools. The first part focuses on the development of an electronic monograph of the papaya family (Caricaceae) using existing infrastructures of Information Technology (IT) and bioinformatic tools that together set the stage for a new era of systematics. Based on the plastid and nuclear DNA data, the author inferred historical processes in the second part that may have shaped the evolution of the Caricaceae and explain their current geographic distribution. The last part is dedicated to the evolution of chromosome numbers in the Caricaceae and includes counts for species from three genera (Cylicomorpha, Horovitzia, Jarilla) that have never been investigated before.
(source: Nielsen Book Data)
Book
online resource (xiv, 396 pages) : illustrations (some color) ; 26 cm
  • Neuronal cell death : an overview of its different forms in central and peripheral neurons / Laura Lossi, Claudia Castagna, and Adalberto Merighi
  • Cell volume regulation monitored with combined epifluorescence and digital holographic microscopy / Nicolas Pavillon and Pierre Marquet
  • Flow cytometric analysis of DNA synthesis and apoptosis in central nervous system using fresh cell nuclei / Noelia López-Sánchez and José M. Frade
  • Nuclear signs of pre-neurodegeneration / Fernando C. Baltanás ... [et al.]
  • Multi-parametric O2 imaging in three-dimensional neural cell models with the phosphorescent probes / Ruslan I. Dmitriev and Dmitri B. Papkovsky
  • Calcium imaging in neuron cell death / María Calvo, Carlos Villalobos, and Lucía Núñez
  • Monitoring mitochondrial membranes permeability in live neurons and mitochondrial swelling through electron microscopy analysis / Macarena S. Arrázola and Nibaldo C. Inestrosa
  • Real-time visualization of caspase-3 activation by fluorescence resonance energy transfer (FRET) / Silvia Alasia ... [et al.]
  • Design and cloning of short hairpin RNAs (shRNAs) into a lentiviral silencing vector to study the function of selected proteins in neuronal apoptosis / Nadia Canu
  • Genomic analysis using affymetrix standard microarray genechips (169 format) in degenerate murine retina / Sook Hyun Chung , Weiyong Shen , and Mark Gillies
  • Genomic analysis of transcriptional changes underlying neuronal apoptosis / Sebastiano Cavallaro
  • High-throughput cell death assays / Matthew E. Pamenter and Gabriel G. Haddad
  • Staining of dead neurons by the golgi method in autopsy material / Stavros J. Baloyannis
  • Image analysis algorithms for immunohistochemical assessment of cell death / Stan Krajewski ... [et al.]
  • In vitro oxygen-glucose deprivation to study ischemic cell death / Carla I. Tasca, Tharine Dal-Cim, and Helena Cimarosti
  • Laser microbeam targeting of single nerve axons in cell culture / Nicholas Hyun , Linda Z. Shi , and Michael W. Berns
  • Real-time imaging of retinal cell apoptosis by confocal scanning laser ophthalmoscopy / Eduardo M. Normando ... [et al.]
  • Targeted toxicants to dopaminergic neuronal cell death / Huajun Jin ... [et al.]
  • Stem cells, neural progenitors, and engineered stem cells / Raj R. Rao and Shilpa Iyer
  • Herpes simplex virus type 1 (HSV-1)-derived recombinant vectors for gene transfer and gene therapy / Peggy Marconi , Cornel Fraefel, and Alberto L. Epstein
  • Herpes simplex virus type 1 (HSV-1)-derived amplicon vectors for gene transfer and gene therapy / Cornel Fraefel Peggy Marconi, and Alberto L. Epstein
  • Bone marrow transplantation for research and regenerative therapies in the central nervous system / David Díaz, José Ramón Alonso, and Eduardo Weruaga
  • Detection of activated caspase-8 in injured spinal axons by using fluorochrome-labeled inhibitors of caspases (FLICA) / Antón Barreiro-Iglesias and Michael I. Shifman
  • Generation of zebrafish models by CRISPR/Cas9 genome editing / Alexander Hruscha and Bettina Schmid
  • In vivo assessment of neuronal cell death in Drosophila / Pierre Dourlen
  • Drosophila model for studying phagocytosis following neuronal cell death / Boris Shklyar, Flonia Levy-Adam, and Estee Kurant.
  • Neuronal cell death : an overview of its different forms in central and peripheral neurons / Laura Lossi, Claudia Castagna, and Adalberto Merighi
  • Cell volume regulation monitored with combined epifluorescence and digital holographic microscopy / Nicolas Pavillon and Pierre Marquet
  • Flow cytometric analysis of DNA synthesis and apoptosis in central nervous system using fresh cell nuclei / Noelia López-Sánchez and José M. Frade
  • Nuclear signs of pre-neurodegeneration / Fernando C. Baltanás ... [et al.]
  • Multi-parametric O2 imaging in three-dimensional neural cell models with the phosphorescent probes / Ruslan I. Dmitriev and Dmitri B. Papkovsky
  • Calcium imaging in neuron cell death / María Calvo, Carlos Villalobos, and Lucía Núñez
  • Monitoring mitochondrial membranes permeability in live neurons and mitochondrial swelling through electron microscopy analysis / Macarena S. Arrázola and Nibaldo C. Inestrosa
  • Real-time visualization of caspase-3 activation by fluorescence resonance energy transfer (FRET) / Silvia Alasia ... [et al.]
  • Design and cloning of short hairpin RNAs (shRNAs) into a lentiviral silencing vector to study the function of selected proteins in neuronal apoptosis / Nadia Canu
  • Genomic analysis using affymetrix standard microarray genechips (169 format) in degenerate murine retina / Sook Hyun Chung , Weiyong Shen , and Mark Gillies
  • Genomic analysis of transcriptional changes underlying neuronal apoptosis / Sebastiano Cavallaro
  • High-throughput cell death assays / Matthew E. Pamenter and Gabriel G. Haddad
  • Staining of dead neurons by the golgi method in autopsy material / Stavros J. Baloyannis
  • Image analysis algorithms for immunohistochemical assessment of cell death / Stan Krajewski ... [et al.]
  • In vitro oxygen-glucose deprivation to study ischemic cell death / Carla I. Tasca, Tharine Dal-Cim, and Helena Cimarosti
  • Laser microbeam targeting of single nerve axons in cell culture / Nicholas Hyun , Linda Z. Shi , and Michael W. Berns
  • Real-time imaging of retinal cell apoptosis by confocal scanning laser ophthalmoscopy / Eduardo M. Normando ... [et al.]
  • Targeted toxicants to dopaminergic neuronal cell death / Huajun Jin ... [et al.]
  • Stem cells, neural progenitors, and engineered stem cells / Raj R. Rao and Shilpa Iyer
  • Herpes simplex virus type 1 (HSV-1)-derived recombinant vectors for gene transfer and gene therapy / Peggy Marconi , Cornel Fraefel, and Alberto L. Epstein
  • Herpes simplex virus type 1 (HSV-1)-derived amplicon vectors for gene transfer and gene therapy / Cornel Fraefel Peggy Marconi, and Alberto L. Epstein
  • Bone marrow transplantation for research and regenerative therapies in the central nervous system / David Díaz, José Ramón Alonso, and Eduardo Weruaga
  • Detection of activated caspase-8 in injured spinal axons by using fluorochrome-labeled inhibitors of caspases (FLICA) / Antón Barreiro-Iglesias and Michael I. Shifman
  • Generation of zebrafish models by CRISPR/Cas9 genome editing / Alexander Hruscha and Bettina Schmid
  • In vivo assessment of neuronal cell death in Drosophila / Pierre Dourlen
  • Drosophila model for studying phagocytosis following neuronal cell death / Boris Shklyar, Flonia Levy-Adam, and Estee Kurant.
Medical Library (Lane)
Status of items at Medical Library (Lane)
Medical Library (Lane) Status
Check Lane Library catalog for status
SPRINGER PROTOCOLS Unknown
Book
online resource (xii, 351 pages) : illustrations
  • Visualization of lncRNA by single-molecule fluorescence in situ hybridization / Margaret Dunagin , Moran N. Cabili , John Rinn , and Arjun Raj
  • Super-resolution imaging of nuclear bodies by STED microscopy / Yasushi Okada and Shinichi Nakagawa
  • High-resolution 3D DNA FISH using plasmid probes and computational correction of optical aberrations to study chromatin structure at the sub-megabase scale / Luca Giorgetti, Tristan Piolot, and Edith Heard
  • Time-lapse imaging of nuclear bodies / Saskia Hutten, Samuel Swift, and Angus I. Lamond
  • Visualization of nucleic acids with synthetic exciton-controlled fluorescent oligonucleotide probes / Dan Ohtan Wang and Akimitsu Okamoto
  • Live CLEM imaging to analyze nuclear structures at high resolution / Tokuko Haraguchi, Hiroko Osakada, and Takako Koujin
  • Ultrastructural analysis of nuclear bodies using electron microscopy / Sylvie Souquere and Gérard Pierron
  • Analyses of nuclear proteins and nucleic acid structures using atomic force microscopy / Jamie L. Gilmore [and eight others]
  • Genome-wide co-localization screening of nuclear body components using a fluorescently tagged FLJ cDNA clone library / Tetsuro Hirose and Naoki Goshima
  • Purification of specific chromatin regions using oligonucleotides : capture hybridization analysis of RNA targets (CHART) / Christopher P. Davis and Jason A. West
  • RNA antisense purification (RAP) for mapping RNA interactions with chromatin / Jesse Engreitz, Eric S. Lander, and Mitchell Guttman
  • In situ dissection of RNA functional subunits by domain-specific chromatin isolation by RNA purification (dChIRP) / Jeffrey J. Quinn and Howard Y. Chang
  • Extracting, enriching, and identifying nuclear body sub-complexes using label-based quantitative mass spectrometry / Archa Fox [and three othrs]
  • Studying RNA-binding protein interactions with target mRNAs in eukaryotic cells : native ribonucleoprotein immunoprecipitation (RIP) assays / Joseph A. Cozzitorto [and eight others]
  • Cross-linking and immunoprecipitation of nuclear RNA-binding proteins / Quan Li , Yuri Uemura , and Yukio Kawahara
  • Purification of noncoding RNA and bound proteins using FLAG peptide-conjugated antisense-oligonucleotides / Shungo Adachi and Tohru Natsume
  • MMCT-mediated chromosome engineering technique applicable to functional analysis of lncRNA and nuclear dynamics / Makiko Meguro-Horike and Shin-ichi Horike
  • Reconstitution of nucleocytoplasmic transport using digitonin-permeabilized cells / Shingo Kose, Tomoko Funakoshi, and Naoko Imamoto
  • Genome-wide analysis of long noncoding RNA turnover / Hidenori Tani [and eight others]
  • Knockdown of nuclear-retained long noncoding RNAs using modified DNA antisense oligonucleotides / Xinying Zong [and six others]
  • siRNA screening of nuclear proteins / Yuko Hasegawa and Shinichi Nakagawa.
  • Visualization of lncRNA by single-molecule fluorescence in situ hybridization / Margaret Dunagin , Moran N. Cabili , John Rinn , and Arjun Raj
  • Super-resolution imaging of nuclear bodies by STED microscopy / Yasushi Okada and Shinichi Nakagawa
  • High-resolution 3D DNA FISH using plasmid probes and computational correction of optical aberrations to study chromatin structure at the sub-megabase scale / Luca Giorgetti, Tristan Piolot, and Edith Heard
  • Time-lapse imaging of nuclear bodies / Saskia Hutten, Samuel Swift, and Angus I. Lamond
  • Visualization of nucleic acids with synthetic exciton-controlled fluorescent oligonucleotide probes / Dan Ohtan Wang and Akimitsu Okamoto
  • Live CLEM imaging to analyze nuclear structures at high resolution / Tokuko Haraguchi, Hiroko Osakada, and Takako Koujin
  • Ultrastructural analysis of nuclear bodies using electron microscopy / Sylvie Souquere and Gérard Pierron
  • Analyses of nuclear proteins and nucleic acid structures using atomic force microscopy / Jamie L. Gilmore [and eight others]
  • Genome-wide co-localization screening of nuclear body components using a fluorescently tagged FLJ cDNA clone library / Tetsuro Hirose and Naoki Goshima
  • Purification of specific chromatin regions using oligonucleotides : capture hybridization analysis of RNA targets (CHART) / Christopher P. Davis and Jason A. West
  • RNA antisense purification (RAP) for mapping RNA interactions with chromatin / Jesse Engreitz, Eric S. Lander, and Mitchell Guttman
  • In situ dissection of RNA functional subunits by domain-specific chromatin isolation by RNA purification (dChIRP) / Jeffrey J. Quinn and Howard Y. Chang
  • Extracting, enriching, and identifying nuclear body sub-complexes using label-based quantitative mass spectrometry / Archa Fox [and three othrs]
  • Studying RNA-binding protein interactions with target mRNAs in eukaryotic cells : native ribonucleoprotein immunoprecipitation (RIP) assays / Joseph A. Cozzitorto [and eight others]
  • Cross-linking and immunoprecipitation of nuclear RNA-binding proteins / Quan Li , Yuri Uemura , and Yukio Kawahara
  • Purification of noncoding RNA and bound proteins using FLAG peptide-conjugated antisense-oligonucleotides / Shungo Adachi and Tohru Natsume
  • MMCT-mediated chromosome engineering technique applicable to functional analysis of lncRNA and nuclear dynamics / Makiko Meguro-Horike and Shin-ichi Horike
  • Reconstitution of nucleocytoplasmic transport using digitonin-permeabilized cells / Shingo Kose, Tomoko Funakoshi, and Naoko Imamoto
  • Genome-wide analysis of long noncoding RNA turnover / Hidenori Tani [and eight others]
  • Knockdown of nuclear-retained long noncoding RNAs using modified DNA antisense oligonucleotides / Xinying Zong [and six others]
  • siRNA screening of nuclear proteins / Yuko Hasegawa and Shinichi Nakagawa.
Medical Library (Lane)
Status of items at Medical Library (Lane)
Medical Library (Lane) Status
Check Lane Library catalog for status
SPRINGER PROTOCOLS Unknown