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1 online resource (p. 1-17 ): digital, PDF file.
As model organisms filamentous fungi have been important since the beginning of modern biological inquiry and have benefitted from open data since the earliest genetic maps were shared. From early origins in simple Mendelian genetics of mating types, parasexual genetics of colony colour, and the foundational demonstration of the segregation of a nutritional requirement, the contribution of research systems utilising filamentous fungi has spanned the biochemical genetics era, through the molecular genetics era, and now are at the very foundation of diverse omics approaches to research and development. Fungal model organisms have come from most major taxonomic groups although Ascomycete filamentous fungi have seen the most major sustained effort. In addition to the published material about filamentous fungi, shared molecular tools have found application in every area of fungal biology. Likewise, shared data has contributed to the success of model systems. Furthermore, the scale of data supporting research with filamentous fungi has grown by 10 to 12 orders of magnitude. From genetic to molecular maps, expression databases, and finally genome resources, the open and collaborative nature of the research communities has assured that the rising tide of data has lifted all of the research systems together.
1 online resource ( xiii, 303 pages) : illustrations (chiefly color).
  • Part I Biochemistry and Physiology.- Chapter 1 Evolutionary Origin of Euglena.- Chapter 2 The Mitochondrion of Euglena gracilis.- Chapter 3 C2 Metabolism in Euglena.- Chapter 4 Biochemistry and Physiology of Reactive Oxygen Species in Euglena. Chapter 5 Biochemistry and Physiology of Vitamins in Euglena .- Chapter 6 Biochemistry and Physiology of Heavy Metal Resistance and Accumulation in Euglena.- Part II Cell and Molecular Biology.- Chapter 7 Euglena gracilis Genomes and Transcriptome: Organelles, Nuclear Genome Assembly Strategies, Initial Features.- Chapter 8 Euglena transcript processing.- Chapter 9 Photo and Nutritional Regulation of Euglena Organelle Development.- Chapter 10 Protein targeting to Euglena chloroplasts.- Chapter 11 Photomovement in Euglena.- Chapter 12 Gravitaxis in Euglena.- Part III Biotechnology.- Chapter 13 Wax Ester Fermentation and Its Application for Biofuel Production.- Chapter 14 Large-scale Cultivation of Euglena.
  • (source: Nielsen Book Data)9783319549088 20170621
This much-needed book is the first definitive volume on Euglena in twenty-fire years, offering information on its atypical biochemistry, cell and molecular biology, and potential biotechnology applications. This volume gathers together contributions from well-known experts, who in many cases played major roles in elucidating the phenomenon discussed. Presented in three parts, the first section of this comprehensive book describes novel biochemical pathways which in some instances have an atypical subcellular localization. The second section details atypical cellular mechanisms of organelle protein import, organelle nuclear genome interdependence, gene regulation and expression that provides insights into the evolutionary origins of eukaryotic cells. The final section discusses how biotechnologists have capitalized on the novel cellular and biochemical features of Euglena to produce value added products. Euglena: Biochemistry, Cell and Molecular Biology will provide essential reading for cell and molecular biologists with interests in evolution, novel biochemical pathways, organelle biogenesis and algal biotechnology. Readers will come away from this volume with a full understanding of the complexities of the Euglena as well as new realizations regarding the diversity of cellular processes yet to be discovered.
(source: Nielsen Book Data)9783319549088 20170621
EBSCOhost Access limited to 1 user
1 online resource (1 streaming video file (31 min.) : color, sound).
  • Contents: Future discovery of CNVs
  • Genotyping
  • Inversion and deletion
  • 17q21.31 targeted sequencing
  • Application of next-generation sequencing technology
  • Personalized duplication or CNV map
  • Long read sequencing technology
  • Single-molecule, real-time detection of structural variation (SMRT-SV)
  • Full-spectrum of human genetic variation.
1 online resource (2,080 pages) : illustrations.
  • Fundamental concepts and theories
  • Development and design methodologies
  • Tools and technologies
  • Utilization and application
  • Issues and challenges
  • Emerging trends.
Medical imaging has transformed the ways in which various conditions, injuries, and diseases are identified, monitored, and treated. As various types of digital visual representations continue to advance and improve, new opportunities for their use in medical practice will likewise evolve. Medical Imaging: Concepts, Methodologies, Tools, and Applications presents a compendium of research on digital imaging technologies in a variety of healthcare settings. This multi-volume work contains practical examples of implementation, emerging trends, case studies, and technological innovations essential for using imaging technologies for making medical decisions. This comprehensive publication is an essential resource for medical practitioners, digital imaging technologists, researchers, and medical students.
(source: Nielsen Book Data)9781522505716 20161213
1 online resource.
EBSCOhost Access limited to 1 user
1 online resource (Article No. 084002 ): digital, PDF file.
Electrostatic interactions between DNA molecules have been extensively studied experimentally and theoretically, but several aspects (e.g. its role in determining the pitch of the cholesteric DNA phase) still remain unclear. Here, we performed large-scale all-atom molecular dynamics simulations in explicit water and 150 mM sodium chloride, to reconstruct the potential of mean force (PMF) of two DNA oligomers 24 base pairs long as a function of their interaxial angle and intermolecular distance. We find that the potential of mean force is dominated by total DNA charge, and not by the helical geometry of its charged groups. The theory of homogeneously charged cylinders fits well all our simulation data, and the fit yields the optimal value of the total compensated charge on DNA to ≈65% of its total fixed charge (arising from the phosphorous atoms), close to the value expected from Manning's theory of ion condensation. The PMF calculated from our simulations does not show a significant dependence on the handedness of the angle between the two DNA molecules, or its size is on the order of $1{{k}_{\text{B}}}T$ . Thermal noise for molecules of the studied length seems to mask the effect of detailed helical charge patterns of DNA. The fact that in monovalent salt the effective interaction between two DNA molecules is independent on the handedness of the tilt may suggest that alternative mechanisms are required to understand the cholesteric phase of DNA.
1 online resource.
  • Front Cover; Western Blotting Guru; Copyright Page; Contents; Preface; Note to the Reader; 1 Introduction; 1.1 What Is Western Blotting?; 1.2 A Bit of History; 2 Procedure; 2.1 Sample Preparation; 2.2 Gel Electrophoresis; 2.2.1 Gel Selection and Preparation; 2.2.2 Running the Gels; 2.2.3 Molecular Weight Markers; 2.3 Protein Transfer; 2.3.1 Transfer Setup; 2.3.2 Choosing the Right Membrane; 2.3.3 Blotting Paper; 2.3.4 Transfer Buffer; 2.3.5 Transfer Power Settings; 2.3.6 Visualization of Proteins After Transfer; 2.4 Blocking the Membrane; 2.5 Primary Antibodies; 2.6 Secondary Antibodies
  • 2.7 Blot Washes2.8 Developing Western Blots; 3 Good Practices; 4 Optimization and Troubleshooting; 4.1 Optimization Rules; 4.2 General Optimization Strategies; 4.2.1 Antibody Concentration; 4.2.2 Antibody Incubation Times; 4.2.3 Wash Stringency; 4.2.4 Blocking; 4.2.5 Preclear the Antibodies; 4.2.6 Supplement Antibody Solutions With Nonphosphorylated Peptides; 4.3 Troubleshooting Specific Problems; 4.3.1 Problem Type 1; Subtype 1; Subtype 2; 4.3.2 Problem Type 2; 4.3.3 Problem Type 3; 4.3.4 Problem Type 4; 4.3.5 Problem Type 5; 4.3.6 Problem Type 6; 4.3.7 Problem Type 7
  • 4.3.8 Problem Type 84.3.9 Problem Type 9; 4.3.10 Problem Type 10; 5 Tips and Tricks; 6 Special Cases; 6.1 Quantitative Western Blotting; 6.2 Overlay Assays; 6.3 Phospho-Specific Antibodies; 6.4 Phos-Tag; 6.5 Nonreducing PAGE; 6.6 Dot Blots; 7 Data Analysis, Storage, Retrieval; Appendix A: Buffers and Solutions; Appendix B: SDS-PAGE Gel Tables; Appendix C: SDS-PAGE Protocol; Appendix D: Wet Transfer and Immunoblotting Protocol; Appendix E: Home-Made Enhanced ChemiLuminescence (ECL) Detection; Appendix F: Stripping Protocols; Appendix G: Coomassie Staining Protocol
  • Appendix H: Lysis of Cells Using Native ConditionsAppendix I: Quick Denaturing Lysis Protocol; Appendix J: Protein Tags; Appendix K: Covalent Crosslinking of Antibodies to Beads; References; Back Cover
Western Blotting Guru provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with immunoblotting setup and optimization. As a laboratory guide, it emphasizes the technical aspects of efficiently employing immunoblotting as a tool in molecular biology laboratories. The book covers the basic science underlying immunoblotting and detailed description of the method parameters, followed by good benchtop practices, tips and tricks for obtaining high-quality data and a detailed troubleshooting guide addressing a variety of problem types.
1 online resource (p. 3486-3493 ): digital, PDF file.
Proteins facilitate a wide range of chemical transformations important in soil as well as being a major reservoir of soil nitrogen themselves. The interactions and reactions of proteins with soils and minerals are of key importance to our understanding of their functional persistence in the environment. We combined NMR and EPR spectroscopies to distinguish the reaction of a model protein with a redox active mineral surface (Birnessite, MnO<sub>2</sub>) from its response to a redox neutral phyllosilicate (Kaolinite). Our data demonstrate that birnessite fragments the model protein while kaolinite has little impact on the protein structure. NMR and EPR spectroscopies are shown to be valuable tools to observe these reactions and capture the extent of protein transformation together with the extent of mineral response. These data suggest that mineral surfaces can have both promoting and retarding roles in terrestrial nitrogen cycling, with redox active minerals acting as accelerators by catalyzing the breakdown of proteins and proteinaceous materials while phyllosilicates are more likely to act as preservative media.
1 online resource (1 streaming video file (47 min.) : color, sound).
  • Contents: Properties of GCase: structure and function
  • Enzymology and cell biology of GCase
  • Requirements for GCase activity
  • GCase and its role in Gaucher disease
  • Genotype/Phenotype and molecular correlations
  • Therapies for Gaucher disease (enzymes, genes, chaperones).
8 p. : digital, PDF file.
<i>Escherichia coli</i> plays an important role as a member of the gut microbiota; however, pathogenic strains also exist, including various diarrheagenic <i>E. coli</i> pathotypes and extraintestinal pathogenic <i>E. coli</i> that cause illness outside of the GI-tract. <i>E. coli</i> have traditionally been serotyped using antisera against the ca. 186 O-antigens and 53 H-flagellar antigens. Phenotypic methods, including bacteriophage typing and O- and H- serotyping for differentiating and characterizing <i>E. coli</i> have been used for many years; however, these methods are generally time consuming and not always accurate. Advances in next generation sequencing technologies have made it possible to develop genetic-based subtyping and molecular serotyping methods for <i>E. coli</i>, which are more discriminatory compared to phenotypic typing methods. Furthermore, whole genome sequencing (WGS) of <i>E. coli</i> is replacing established subtyping methods such as pulsedfield gel electrophoresis, providing a major advancement in the ability to investigate food-borne disease outbreaks and for trace-back to sources. Furthermore, a variety of sequence analysis tools and bioinformatic pipelines are being developed to analyze the vast amount of data generated by WGS and to obtain specific information such as O- and H-group determination and the presence of virulence genes and other genetic markers.
1 online resource (206 KB ): digital, PDF file.
This fact sheet provides information about Algal Biofuels Research Laboratory capabilities and applications at NREL's National Bioenergy Center.
1 online resource (Article No. 33079 ): digital, PDF file.
Although aggregation of Aβ amyloid fibrils into plaques in the brain is a hallmark of Alzheimer's Disease (AD), the correlation between amyloid burden and severity of symptoms is weak. One possible reason is that amyloid fibrils are structurally polymorphic and different polymorphs may contribute differentially to disease. However, the occurrence and distribution of amyloid polymorphisms in human brain is poorly documented. Here we seek to fill this knowledge gap by using X-ray microdiffraction of histological sections of human tissue to map the abundance, orientation and structural heterogeneities of amyloid within individual plaques; among proximal plaques and in subjects with distinct clinical histories. A 5 µ x-ray beam was used to generate diffraction data with each pattern arising from a scattering volume of only ~ 450 µ3 , making possible collection of dozens to hundreds of diffraction patterns from a single amyloid plaque. X-ray scattering from these samples exhibited all the properties expected for scattering from amyloid. Amyloid distribution was mapped using the intensity of its signature 4.7 Å reflection which also provided information on the orientation of amyloid fibrils across plaques. Margins of plaques exhibited a greater degree of orientation than cores and orientation around blood vessels frequently appeared tangential. Variation in the structure of Aβ fibrils is reflected in the shape of the 4.7 Å peak which usually appears as a doublet. Variations in this peak correspond to differences between the structure of amyloid within cores of plaques and at their periphery. Examination of tissue from a mismatch case - an individual with high plaque burden but no overt signs of dementia at time of death - revealed a diversity of structure and spatial distribution of amyloid that is distinct from typical AD cases. As a result, we demonstrate the existence of structural polymorphisms among amyloid within and among plaques of a single individual and suggest the existence of distinct differences in the organization of amyloid in subjects with different clinical presentations.
1 online resource (vii, 182 pages) : color illustrations
  • Preface; Contents; 1: The Protein Data Bank; 2: Seeing Is Believing: Methods of Structure Solution; 3: Visualizing the Invisible World of Molecules; 4: The Twists and Turns of DNA; 5: The Central Dogma; 6: The Secret of Life: The Genetic Code; 7: Evolution in Action; 8: How Evolution Shapes Proteins; 9: The Universe of Protein Folds; 10: Order and Chaos in Protein Structure; 11: Molecular Electronics; 12: Green Energy; 13: Peak Performance; 14: Cellular Signaling Networks; 15: GPCRs Revealed; 16: Signaling with Hormones; 17: Single-Molecule Chemistry: Enzyme Action and the Transition State
  • 18: Seven Wonders of the World of Enzymes One: Perfect Enzymes; Two: Induced Fit; Three: Form-Fitting Active Sites; Four: Allostery; Five: Substrate Channeling; Six: Chemical Cofactors; Seven: Ribozymes; 19: Building Bodies; 20: Coloring the Biological World; 21: Amazing Antibodies; 22: Attack and Defense:Weapons of the ImmuneSystem; 23: Reconstructing HIV
This book will take an evidence-based approach to current knowledge about biomolecules and their place in our lives, inviting readers to explore how we know what we know, and how current gaps in knowledge may influence the way we approach the information. Biomolecular science is increasingly important in our everyday life, influencing the choices we make about our diet, our health, and our wellness. Often, however, information about biomolecular science is presented as a list of immutable facts, discouraging critical thought. The book will introduce the basic tools of structural biology, supply real-life examples, and encourage critical thought about aspects of biology that are still not fully understood.
1 online resource (pages ; cm.) :

15. Biotechnology [2016]

1 online resource (xv, 833 pages) : color illustrations.
  • Basics of biotechnology
  • DNA, RNA, and protein
  • Recombinant DNA technology
  • DNA synthesis in vivo and in vitro
  • RNA-based technologies
  • Immune technology
  • Nanobiotechnology
  • Genomics and gene expression
  • Proteomics
  • Recombinant proteins
  • Protein engineering
  • Environmental biotechnology
  • Synthetic biology
  • From cell phones to cyborgs
  • Transgenic plants and plant biotechnology
  • Transgenic animals
  • Inherited defects and gene therapy
  • Cloning and stem cells
  • Cancer
  • Aging and apoptosis
  • Viral and prion infections
  • Biological warfare : infectious disease and bioterrorism
  • Forensic molecular biology
  • Bioethics in biotechnology.
1 online resource (1 streaming video file (24 min.) : color, sound).
  • Contents: Uniformitarianism, Lamarckism and Darwin's theory
  • Mendel’s work and the science of genetics
  • The modern synthesis: a mechanistic basis for variation and heredity
  • Molecular biology: the age of biological information
  • Rethinking gradual progressive evolution
  • Evolutionary biology in the age of genomics
  • Eugenics, the dark chapter of evolutionary biology
  • Group-level selection and evolution of morality.
1 online resource (1 streaming video file (36 min.) : color, sound).
  • Contents: Cardiac aging in human and animal models
  • Molecular mechanisms for cardiac aging
  • Recent advances on potential interventions for cardiac aging
  • Future perspectives of cardiac aging interventions.
1 online resource (1 streaming video file (16 min.) : color, sound).
  • Contents: Niche construction: definitions and examples
  • The endomembrane system
  • Advent of cholesterol depended on oxygen
  • Ecosystems and the process of death
  • Internal niche construction
  • The swim-bladder, the lung and PTHrP
  • Niche construction and epigenetics interactions
  • Gaia hypothesis.
1 online resource (10 p.) : digital, PDF file.
Similar to ruminants, swine have been shown to be a reservoir for Shiga toxin-producing Escherichia coli (STEC), and pork products have been linked with outbreaks associated with STEC O157 and O111:H-. STEC strains, isolated in a previous study from fecal samples of late-finisher pigs, belonged to a total of 56 serotypes, including O15:H27, O91:H14, and other serogroups previously associated with human illness. The isolates were tested by polymerase chain reaction (PCR) and a high-throughput real-time PCR system to determine the Shiga toxin (Stx) subtype and virulence-associated and putative virulence-associated genes they carried. Select STEC strains were further analyzed using a Minimal Signature E. coli Array Strip. As expected, stx<sub>2e</sub> (81%) was the most common Stx variant, followed by stx<sub>1a</sub> (14%), stx<sub>2d</sub> (3%), and stx<sub>1c</sub> (1%). The STEC serogroups that carried stx<sub>2d</sub> were O15:H27, O159:H16 and O159:H-. Similar to stx<sub>2a</sub> and stx<sub>2c</sub>, the stx<sub>2d</sub> variant is associated with development of hemorrhagic colitis and hemolytic uremic syndrome, and reports on the presence of this variant in STEC strains isolated from swine are lacking. Moreover, the genes encoding heat stable toxin (estIa) and enteroaggregative E. coli heat stable enterotoxin-1 (astA) were commonly found in 50 and 44% of isolates, respectively. The hemolysin genes, hlyA and ehxA, were both detected in 7% of the swine STEC strains. Although the eae gene was not found, other genes involved in host cell adhesion, including lpfA<sub>O113</sub> and paa were detected in more than 50% of swine STEC strains, and a number of strains also carried iha, lpfA<sub>O26</sub>, lpfA<sub>O157</sub>, fedA, orfA, and orfB. Furthermore, the present work provides new insights on the distribution of virulence factors among swine STEC strains and shows that swine may carry Stx1a-, Stx2e-, or Stx2d-producing E. coli with virulence gene profiles associated with human infections.
1 online resource (1 streaming video file (52 min.) : color, sound).
  • Contents: Application of new diagnostic techniques in clinical medicine, mainly in molecular cytogenetics of neoplasia
  • Relevant chromosomal abnormalities that impact the diagnosis and prognosis of patients with acute lymphoblastic leukemia and acute myeloblastic leukemia.