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Book
online resource (xii, 367 pages, 12 unnumbered pages of plates) : illustrations
  • Durban and Johannesburg
  • Cape Town
  • Cambridge
  • The first visit to Israel
  • Back to Cambridge
  • Birkbeck-1
  • Birkbeck-2
  • The Laboratory of Molecular Biology
  • Image analysis in electron microscopy
  • Spherical virus structure
  • Tobacco mosaic virus
  • From thon rings to modern dance
  • Tomography
  • Transfer RNA
  • Chromatin and nucleosomes
  • Zinc fingers
  • Peterhouse
  • Director of the Laboratory of Molecular Biology
  • Presidency of the Royal Society
  • Ben Gurion University
  • Prizes
  • Envoi.
The atomic structures of macromolecules provide the key to understanding how life works. Aaron Klug led the way in the development of methods for solving such structures and is one of the pioneers of structural molecular biology. He was awarded a Nobel Prize in 1982 for his work. Illuminating both his personal life and scientific achievements, this unique biography begins with Klug's youth in Durban and his studies at Johannesburg, Cape Town and then Trinity College, Cambridge. Holmes proceeds to explore Klug's career from his work on the structure of viruses with Rosalind Franklin at Birkbeck College, London to his time as Director of the MRC Laboratory of Molecular Biology (LMB) in Cambridge and as President of the Royal Society. Drawing on their long-term collaboration, interviews and unique access to Klug's archives, Holmes provides a fascinating account of an innovative man and his place in the history of structural molecular biology.
Medical Library (Lane)
Book
1 online resource (p. 67-83) : digital, PDF file.
As model organisms filamentous fungi have been important since the beginning of modern biological inquiry and have benefitted from open data since the earliest genetic maps were shared. From early origins in simple Mendelian genetics of mating types, parasexual genetics of colony colour, and the foundational demonstration of the segregation of a nutritional requirement, the contribution of research systems utilising filamentous fungi has spanned the biochemical genetics era, through the molecular genetics era, and now are at the very foundation of diverse omics approaches to research and development. Fungal model organisms have come from most major taxonomic groups although Ascomycete filamentous fungi have seen the most major sustained effort. In addition to the published material about filamentous fungi, shared molecular tools have found application in every area of fungal biology. Likewise, shared data has contributed to the success of model systems. Furthermore, the scale of data supporting research with filamentous fungi has grown by 10 to 12 orders of magnitude. From genetic to molecular maps, expression databases, and finally genome resources, the open and collaborative nature of the research communities has assured that the rising tide of data has lifted all of the research systems together.
Book
1 online resource ( xiii, 303 pages) : illustrations (chiefly color).
  • Part I Biochemistry and Physiology.- Chapter 1 Evolutionary Origin of Euglena.- Chapter 2 The Mitochondrion of Euglena gracilis.- Chapter 3 C2 Metabolism in Euglena.- Chapter 4 Biochemistry and Physiology of Reactive Oxygen Species in Euglena. Chapter 5 Biochemistry and Physiology of Vitamins in Euglena .- Chapter 6 Biochemistry and Physiology of Heavy Metal Resistance and Accumulation in Euglena.- Part II Cell and Molecular Biology.- Chapter 7 Euglena gracilis Genomes and Transcriptome: Organelles, Nuclear Genome Assembly Strategies, Initial Features.- Chapter 8 Euglena transcript processing.- Chapter 9 Photo and Nutritional Regulation of Euglena Organelle Development.- Chapter 10 Protein targeting to Euglena chloroplasts.- Chapter 11 Photomovement in Euglena.- Chapter 12 Gravitaxis in Euglena.- Part III Biotechnology.- Chapter 13 Wax Ester Fermentation and Its Application for Biofuel Production.- Chapter 14 Large-scale Cultivation of Euglena.
  • (source: Nielsen Book Data)9783319549088 20170621
This much-needed book is the first definitive volume on Euglena in twenty-fire years, offering information on its atypical biochemistry, cell and molecular biology, and potential biotechnology applications. This volume gathers together contributions from well-known experts, who in many cases played major roles in elucidating the phenomenon discussed. Presented in three parts, the first section of this comprehensive book describes novel biochemical pathways which in some instances have an atypical subcellular localization. The second section details atypical cellular mechanisms of organelle protein import, organelle nuclear genome interdependence, gene regulation and expression that provides insights into the evolutionary origins of eukaryotic cells. The final section discusses how biotechnologists have capitalized on the novel cellular and biochemical features of Euglena to produce value added products. Euglena: Biochemistry, Cell and Molecular Biology will provide essential reading for cell and molecular biologists with interests in evolution, novel biochemical pathways, organelle biogenesis and algal biotechnology. Readers will come away from this volume with a full understanding of the complexities of the Euglena as well as new realizations regarding the diversity of cellular processes yet to be discovered.
(source: Nielsen Book Data)9783319549088 20170621
EBSCOhost Access limited to 1 user
Video
1 online resource (1 streaming video file (34 min.) : color, sound).
  • Contents: History of discovery of The complement system
  • Evolution of complement
  • The alternative complement pathway
  • Defects and diseases associated with complement
  • Complement and inflammation.
Book
1 online resource.
  • Part I: Self/Nonself Evolution, â A new view of how MHC class I molecules fight disease: generalists and specialists.- Evolution and diversity of defensins in vertebrates.- Interdependencies between the adaptation and interference modules guide efficient CRISPR-Cas immunity.- How the other half lives: CRISPR-Cas's influence on bacteriophages.- Hidden Silent Codes in Viral Genomes.- Self and Non-Self from a Genomic Perspective: Transposable Elements.- Mammalian-specific traits generated by LTR retrotransposon-derived SIRH genes.- Part II: Species Evolution and Evolution of Complex Traits, The life history of domesticated genes illuminates the evolution of novel mammalian genes.- Evolution of Complex Traits in Human Populations.- The descent of bison.- Convergent and parallel evolution in early Glires (Mammalia).- Reductive evolution of apicomplexan parasites from phototrophic an-cestors.- Part III : Methods and Concepts, Evolution of milk oligosaccharides and their function in monotremes and marsupials.- Mechanistic Models of Protein Evolution.- Genome-wide screens for molecular convergent evolution in mammals.- Assessing evolutionary potential in tree species through ecology-informed genome screening.- Evolutionary constraints on coding sequences at the nucleotidic level: a statistical physics approach.- Case studies of seven gene families with unusual high retention rate since the Vertebrate and Teleost Whole Genome Duplications.
  • (source: Nielsen Book Data)9783319615684 20171009
This book presents 19 selected contributions to the 20th Evolutionary Biology Meeting in Marseille, which took place in September 2016. They are grouped under the following major themes:* Self/Nonself Evolution* Species Evolution and Evolution of Complex Traits* Methods and Concepts The aims of the annual meetings in Marseille - which bring together leading evolutionary biologists and other scientists using evolutionary biology concepts, e.g. for medical research - are to promote the exchange of ideas and to encourage interdisciplinary collaborations. Offering a revealing overview of the latest findings in the field of evolutionary biology, this book represents an invaluable source of information for scientists, teachers and advanced students alike.
(source: Nielsen Book Data)9783319615684 20171009
Video
1 online resource (1 streaming video file (31 min.) : color, sound).
  • Contents: Future discovery of CNVs
  • Genotyping
  • Inversion and deletion
  • 17q21.31 targeted sequencing
  • Application of next-generation sequencing technology
  • Personalized duplication or CNV map
  • Long read sequencing technology
  • Single-molecule, real-time detection of structural variation (SMRT-SV)
  • Full-spectrum of human genetic variation.
Book
PDF-file: 6 pages; size: 3.4 Mbytes
Abstract not provided
Book
1 online resource (Article No. 15441) : digital, PDF file.
Mg/Ca ratios of planktic foraminifera are commonly used to reconstruct past ocean temperatures. However, intrashell Mg/Ca ratios exhibit a pattern of alternating high and low Mg-bands in many species. Whereas mechanisms controlling Mg variability are poorly constrained, recent experiments demonstrate that it is paced by the diurnal light/dark cycle in Orbulina universa, which forms a terminal shell of simple spherical geometry. It is unknown whether Mg-heterogeneity is diurnally paced in species with complex shell morphologies, or is the result of growth processes. Here, we show that high Mg/Ca-calcite also forms at night in cultured specimens of the multi-chambered planktic foraminifera Neogloboquadrina dutertrei. Our results demonstrate that N. dutertrei adds a significant amount of calcite, and nearly all Mg-bands, after the final chamber forms. Furthermore, these results have implications for interpreting patterns of calcification in N. dutertrei, and possibly other foraminifera species, and suggests diurnal Mg-banding is an intrinsic component of biomineralization in planktic foraminifera.
Book
1 online resource (Article No. 183) : digital, PDF file.
Understanding the biological mechanisms related to lipids and glycolipids is challenging due to the vast number of possible isomers. Mass spectrometry (MS) measurements are currently the dominant approach for studying and providing detailed information on lipid and glycolipid structures. However, difficulties in distinguishing many structural isomers (e.g. distinct acyl chain positions, double bond locations, as well as glycan isomers) inhibit the understanding of their biological roles. Here we utilized ultra-high resolution ion mobility spectrometry (IMS) separations based upon the use of traveling waves in a serpentine long path length multi-pass Structures for Lossless Manipulations (SLIM) to enhance isomer resolution. The multi-pass arrangement allowed separations ranging from ~16 m (1 pass) to ~470 m (32 passes) to be investigated for the distinction of lipids and glycolipids with extremely small structural differences. Lastly, these ultra-high resolution SLIM IMS-MS analyses provide a foundation for exploring and better understanding isomer specific biological and disease processes.
Book
1 online resource (Article No. 40555) : digital, PDF file.
Lung immaturity is a major cause of morbidity and mortality in premature infants. Understanding the molecular mechanisms driving normal lung development could provide insights on how to ameliorate disrupted development. While transcriptomic and proteomic analyses of normal lung development have been previously reported, characterization of changes in the lipidome is lacking. Lipids play significant roles in the lung, such as dipalmitoylcholine in pulmonary surfactant; however, many of the roles of specific lipid species in normal lung development, as well as in disease states, are not well defined. In this study, we used liquid chromatography-mass spectrometry (LC-MS/MS) to investigate the murine lipidome during normal postnatal lung development. Lipidomics analysis of lungs from post-natal day 7, day 14 and 6-8 week mice (adult) identified 928 unique lipids across 21 lipid subclasses, with dramatic alterations in the lipidome across developmental stages. Our data confirmed previously recognized aspects of post-natal lung development and revealed several insights, including in sphingolipid-mediated apoptosis, inflammation and energy storage/usage. Complementary proteomics, metabolomics and chemical imaging corroborated these observations. Finally, this multi-omic view provides a unique resource and deeper insight into normal pulmonary development.
Book
1 online resource (2,080 pages) : illustrations.
  • Fundamental concepts and theories
  • Development and design methodologies
  • Tools and technologies
  • Utilization and application
  • Issues and challenges
  • Emerging trends.
Medical imaging has transformed the ways in which various conditions, injuries, and diseases are identified, monitored, and treated. As various types of digital visual representations continue to advance and improve, new opportunities for their use in medical practice will likewise evolve. Medical Imaging: Concepts, Methodologies, Tools, and Applications presents a compendium of research on digital imaging technologies in a variety of healthcare settings. This multi-volume work contains practical examples of implementation, emerging trends, case studies, and technological innovations essential for using imaging technologies for making medical decisions. This comprehensive publication is an essential resource for medical practitioners, digital imaging technologists, researchers, and medical students.
(source: Nielsen Book Data)9781522505716 20161213
Book
1 online resource (xviii, 740 pages) : illustrations (chiefly color).
ProQuest Ebook Central Access limited to 3 simultaneous users
Book
1 online resource.
EBSCOhost Access limited to 1 user
Book
1 online resource (Article No. 13924) : digital, PDF file.
Microbial phototrophs, key primary producers on Earth, use H<sub>2</sub>O, H<sub>2</sub>, H<sub>2</sub>S and other reduced inorganic compounds as electron donors. Here we describe a form of metabolism linking anoxygenic photosynthesis to anaerobic respiration that we call ‘syntrophic anaerobic photosynthesis’. We show that photoautotrophy in the green sulfur bacterium Prosthecochloris aestaurii can be driven by either electrons from a solid electrode or acetate oxidation via direct interspecies electron transfer from a heterotrophic partner bacterium, Geobacter sulfurreducens. Photosynthetic growth of P. aestuarii using reductant provided by either an electrode or syntrophy is robust and light-dependent. In contrast, P. aestuarii does not grow in co-culture with a G. sulfurreducens mutant lacking a trans-outer membrane porin-cytochrome protein complex required for direct intercellular electron transfer. Syntrophic anaerobic photosynthesis is therefore a carbon cycling process that could take place in anoxic environments. Lastly, this process could be exploited for biotechnological applications, such as waste treatment and bioenergy production, using engineered phototrophic microbial communities.
Book
1 online resource (1.2 MB) : digital, PDF file.
Plant cell walls have three primary components, cellulose, hemicellulose, and lignin, the latter of which is a recalcitrant, aromatic heteropolymer that provides structure to plants, water and nutrient transport through plant tissues, and a highly effective defense against pathogens. Overcoming the recalcitrance of lignin is key to effective biomass deconstruction, which would in turn enable the use of biomass as a feedstock for industrial processes. Our understanding of lignin structure in the plant cell wall is hampered by the limitations of the available lignin forcefields, which currently only account for a single linkage between lignins and lack explicit parameterization for emerging lignin structures both from natural variants and engineered lignin structures. Since polymerization of lignin occurs via radical intermediates, multiple C-O and C-C linkages have been isolated , and the current force field only represents a small subset of lignin the diverse lignin structures found in plants. In order to take into account the wide range of lignin polymerization chemistries, monomers and dimer combinations of C-, H-, G-, and S-lignins as well as with hydroxycinnamic acid linkages were subjected to extensive quantum mechanical calculations to establish target data from which to build a complete molecular mechanics force field tuned specifically for diverse lignins. This was carried out in a GPU-accelerated global optimization process, whereby all molecules were parameterized simultaneously using the same internal parameter set. By parameterizing lignin specifically, we are able to more accurately represent the interactions and conformations of lignin monomers and dimers relative to a general force field. This new force field will enables computational researchers to study the effects of different linkages on the structure of lignin, as well as construct more accurate plant cell wall models based on observed statistical distributions of lignin that differ between disparate feedstocks, and guide further lignin engineering efforts.
Video
1 online resource (1 streaming video file (51 min.) : color, sound).
  • Contents: Targeted therapy for cancer
  • Adaptive resistance: PI3K pathway
  • Tumor heterogeneity
  • Challenges & need for functional genomics
  • Identification of PIK3R1 driver mutations
  • Human proteomics atlas: reverse phase protein array (RPPA)
  • Checkpoint inhibition as potential targets
  • Synergy, operating model & strategy for combination therapies.
Book
1 online resource (Article No. 084002) : digital, PDF file.
Electrostatic interactions between DNA molecules have been extensively studied experimentally and theoretically, but several aspects (e.g. its role in determining the pitch of the cholesteric DNA phase) still remain unclear. Here, we performed large-scale all-atom molecular dynamics simulations in explicit water and 150 mM sodium chloride, to reconstruct the potential of mean force (PMF) of two DNA oligomers 24 base pairs long as a function of their interaxial angle and intermolecular distance. We find that the potential of mean force is dominated by total DNA charge, and not by the helical geometry of its charged groups. The theory of homogeneously charged cylinders fits well all our simulation data, and the fit yields the optimal value of the total compensated charge on DNA to ≈65% of its total fixed charge (arising from the phosphorous atoms), close to the value expected from Manning's theory of ion condensation. The PMF calculated from our simulations does not show a significant dependence on the handedness of the angle between the two DNA molecules, or its size is on the order of $1{{k}_{\text{B}}}T$ . Thermal noise for molecules of the studied length seems to mask the effect of detailed helical charge patterns of DNA. The fact that in monovalent salt the effective interaction between two DNA molecules is independent on the handedness of the tilt may suggest that alternative mechanisms are required to understand the cholesteric phase of DNA.
Book
1 online resource.
  • Front Cover; Western Blotting Guru; Copyright Page; Contents; Preface; Note to the Reader; 1 Introduction; 1.1 What Is Western Blotting?; 1.2 A Bit of History; 2 Procedure; 2.1 Sample Preparation; 2.2 Gel Electrophoresis; 2.2.1 Gel Selection and Preparation; 2.2.2 Running the Gels; 2.2.3 Molecular Weight Markers; 2.3 Protein Transfer; 2.3.1 Transfer Setup; 2.3.2 Choosing the Right Membrane; 2.3.3 Blotting Paper; 2.3.4 Transfer Buffer; 2.3.5 Transfer Power Settings; 2.3.6 Visualization of Proteins After Transfer; 2.4 Blocking the Membrane; 2.5 Primary Antibodies; 2.6 Secondary Antibodies
  • 2.7 Blot Washes2.8 Developing Western Blots; 3 Good Practices; 4 Optimization and Troubleshooting; 4.1 Optimization Rules; 4.2 General Optimization Strategies; 4.2.1 Antibody Concentration; 4.2.2 Antibody Incubation Times; 4.2.3 Wash Stringency; 4.2.4 Blocking; 4.2.5 Preclear the Antibodies; 4.2.6 Supplement Antibody Solutions With Nonphosphorylated Peptides; 4.3 Troubleshooting Specific Problems; 4.3.1 Problem Type 1; 4.3.1.1 Subtype 1; 4.3.1.2 Subtype 2; 4.3.2 Problem Type 2; 4.3.3 Problem Type 3; 4.3.4 Problem Type 4; 4.3.5 Problem Type 5; 4.3.6 Problem Type 6; 4.3.7 Problem Type 7
  • 4.3.8 Problem Type 84.3.9 Problem Type 9; 4.3.10 Problem Type 10; 5 Tips and Tricks; 6 Special Cases; 6.1 Quantitative Western Blotting; 6.2 Overlay Assays; 6.3 Phospho-Specific Antibodies; 6.4 Phos-Tag; 6.5 Nonreducing PAGE; 6.6 Dot Blots; 7 Data Analysis, Storage, Retrieval; Appendix A: Buffers and Solutions; Appendix B: SDS-PAGE Gel Tables; Appendix C: SDS-PAGE Protocol; Appendix D: Wet Transfer and Immunoblotting Protocol; Appendix E: Home-Made Enhanced ChemiLuminescence (ECL) Detection; Appendix F: Stripping Protocols; Appendix G: Coomassie Staining Protocol
  • Appendix H: Lysis of Cells Using Native ConditionsAppendix I: Quick Denaturing Lysis Protocol; Appendix J: Protein Tags; Appendix K: Covalent Crosslinking of Antibodies to Beads; References; Back Cover
Western Blotting Guru provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with immunoblotting setup and optimization. As a laboratory guide, it emphasizes the technical aspects of efficiently employing immunoblotting as a tool in molecular biology laboratories. The book covers the basic science underlying immunoblotting and detailed description of the method parameters, followed by good benchtop practices, tips and tricks for obtaining high-quality data and a detailed troubleshooting guide addressing a variety of problem types.
Book
1 online resource (p. 3486-3493 ): digital, PDF file.
Proteins facilitate a wide range of chemical transformations important in soil as well as being a major reservoir of soil nitrogen themselves. The interactions and reactions of proteins with soils and minerals are of key importance to our understanding of their functional persistence in the environment. We combined NMR and EPR spectroscopies to distinguish the reaction of a model protein with a redox active mineral surface (Birnessite, MnO<sub>2</sub>) from its response to a redox neutral phyllosilicate (Kaolinite). Our data demonstrate that birnessite fragments the model protein while kaolinite has little impact on the protein structure. NMR and EPR spectroscopies are shown to be valuable tools to observe these reactions and capture the extent of protein transformation together with the extent of mineral response. These data suggest that mineral surfaces can have both promoting and retarding roles in terrestrial nitrogen cycling, with redox active minerals acting as accelerators by catalyzing the breakdown of proteins and proteinaceous materials while phyllosilicates are more likely to act as preservative media.
Video
1 online resource (1 streaming video file (47 min.) : color, sound).
  • Contents: Properties of GCase: structure and function
  • Enzymology and cell biology of GCase
  • Requirements for GCase activity
  • GCase and its role in Gaucher disease
  • Genotype/Phenotype and molecular correlations
  • Therapies for Gaucher disease (enzymes, genes, chaperones).