Book
1 online resource
Experimental Methods in Orthopaedic Biomechanics is the first book in the field that focuses on the practicalities of performing a large variety of in-vitro laboratory experiments. Explanations are thorough, informative, and feature standard lab equipment to enable biomedical engineers to advance from a 'trial and error' approach to an efficient system recommended by experienced leaders. This is an ideal tool for biomedical engineers or biomechanics professors in their teaching, as well as for those studying and carrying out lab assignments and projects in the field. The experienced authors have established a standard that researchers can test against in order to explain the strengths and weaknesses of testing approaches.
Book
1 PDF (xv, 135 pages).
  • 1. Introduction
  • 1.1 Enabling technologies
  • 1.1.1 Camera-based systems
  • 1.1.2 Body worn sensors
  • 1.1.3 Force and pressure-based systems
  • 1.2 Body tracking in context
  • 1.3 Overview
  • 2. Clinical assessment of motor disability
  • 2.1 Introduction
  • 2.2 Tracking disease progression in multiple sclerosis assessment
  • 2.2.1 Contexts and practices in MS assessment with the EDSS
  • 2.2.2 Challenges and characteristics of assessment room
  • 2.2.3 Doctor-patient relationship in assessment
  • 2.2.4 Summary
  • 2.3 Understanding concerns in system design: assess MS system
  • 2.3.1 System overview
  • 2.3.2 Algorithms
  • 2.3.3 Movement exercise protocol
  • 2.3.4 Ensuring standardized movement performance
  • 2.3.5 Framing and standardization, seeing how the machine sees
  • 2.3.6 Representing the movement measure and classification
  • 2.4 Conclusions
  • 3. Self-directed rehabilitation and care
  • 3.1 Introduction
  • 3.2 Facilitating physical activity in chronic musculoskeletal pain
  • 3.3 Technology for chronic pain rehabilitation
  • 3.3.1 Go-with-the-flow: sonification in movement rehabilitation
  • 3.3.2 Transferring to everyday functioning: kinect vs. wearable smartphone as a body-tracking device
  • 3.3.3 Self-directed rehabilitation as process: from clinical facilitation to self-management
  • 3.3.4 Tracking affective states and pain levels
  • 3.4 Exergaming and balance rehabilitation in older adults
  • 3.4.1 Balance and fall risk in older adults
  • 3.4.2 Body-tracking technology for balance training
  • 3.4.3 Designing a balance training game
  • 3.4.4 Understanding rehabilitative game use
  • 3.5 Conclusion
  • 4. Interactions for clinicians
  • 4.1 Introduction
  • 4.2 Sterility and constraints on imaging practices
  • 4.3 Tracking the body of the clinician for enabling touchless interaction with images
  • 4.4 Clinical considerations in gesture design
  • 4.4.1 Clinical constraints on movement in gesture design
  • 4.4.2 Supporting collaboration and control
  • 4.4.3 What actions and body parts to track for the purposes of system control
  • 4.4.4 Engaging and disengaging the system
  • 4.4.5 Feedback and making oneself sensed
  • 4.4.6 Coarse vs. fine-grained control
  • 4.5 Body tracking, gesture, and robotics
  • 4.6 Increasing interaction bandwidth through input modality
  • 4.7 Conclusions
  • 5. Conclusions
  • 5.1 Introduction
  • 5.2 Contextual design
  • 5.2.1 Sensor technology
  • 5.2.2 Data and algorithms
  • 5.2.3 Designing movements
  • 5.2.4 Interface and interaction design
  • 5.2.5 Physical set-up and form factor
  • 5.2.6 Social set-up and practices
  • 5.3 The future
  • Bibliography
  • Author biographies.
Within the context of healthcare, there has been a long-standing interest in understanding the posture and movement of the human body. Gait analysis work over the years has looked to articulate the patterns and parameters of this movement both for a normal healthy body and in a range of movement-based disorders. In recent years, these efforts to understand the moving body have been transformed by significant advances in sensing technologies and computational analysis techniques all offering new ways for the moving body to be tracked, measured, and interpreted. While much of this work has been largely research focused, as the field matures, we are seeing more shifts into clinical practice. As a consequence, there is an increasing need to understand these sensing technologies over and above the specific capabilities to track, measure, and infer patterns of movement in themselves. Rather, there is an imperative to understand how the material form of these technologies enables them also to be situated in everyday healthcare contexts and practices. There are significant mutually interdependent ties between the fundamental characteristics and assumptions of these technologies and the configurations of everyday collaborative practices that are possible them. Our attention then must look to social, clinical, and technical relations pertaining to these various body technologies that may play out in particular ways across a range of different healthcare contexts and stakeholders. Our aim in this book is to explore these issues with key examples illustrating how social contexts of use relate to the properties and assumptions bound up in particular choices of body-tracking technology. We do this through a focus on three core application areas in healthcare-assessment, rehabilitation, and surgical interaction-and recent efforts to apply body-tracking technologies to them.
(source: Nielsen Book Data)9781627054560 20160619
Video
1 online resource (1 streaming video file (36 min.) : color, sound).
  • Contents: Cardiac aging in human and animal models
  • Molecular mechanisms for cardiac aging
  • Recent advances on potential interventions for cardiac aging
  • Future perspectives of cardiac aging interventions.
Book
online resource (x, 438 pages) : color illustrations ; 19 cm
  • Fundamentals / S. Silbernagl and F. Lang
  • Temperature, energy / S. Silbernagl
  • Blood / S. Silbernagl
  • Kidney, salt and water balance / F. Lang
  • Stomach, intestines, liver / S. Silbernagl
  • Heart and circulation / S. Silbernagl
  • Metabolic disorders / S. Silbernagl
  • Hormones / F. Lang
  • Neuromuscular and sensory systems / F. Lang.
Medical Library (Lane)
Book
1 online resource (284 ill., 186 illus. in color. eReference.) : online resource. Digital: text file; PDF.
  • â General aspects, definitions and assessments of PTSD.- PTSD in different conditions, groups, and specific incidents.- PTSD in relation to psychotic conditions or assessments.- Peritraumatic stress.- Biochemical, molecular, and genetic aspects of PTSD.- Neurological and neurogenic aspects of PTSD.- PTSD as a risk factor in causation of diseases and pathology.- PTSD in children and adolescents.- Animal models of PTSD: enhancing understanding.- PTSD in international and comparative perspective.- PTSD and selective conditions of stress and anxiety.- Treatments and therapies of PTSD.
  • (source: Nielsen Book Data)9783319083599 20160912
This is an all-embracing reference that offers analyses and discussions of contemporary issues in the field of PTSD. The book brings together scientific material from leading experts in the field relating to a wide range of important current topics across disciplines. These include the early identification of PTSD and subsequent treatment, to social and behavioral studies, to biochemical, molecular and genetic research. With more than 125 chapters organized in 12 major sections, this is the most complete single resource on PTSD.
(source: Nielsen Book Data)9783319083599 20160912
Video
1 online resource (1 streaming video file (48 min.) : color, sound).
  • Contents: Insights into human aging
  • The compression of morbidity: central thesis
  • Reduction in health risks postpones aging
  • Delay aging by lifelong exercise
  • Exercise and morbidity
  • Actions that postpone aging
  • Multiple controlled longitudinal studies.
Video
1 online resource (1 streaming video file (52 min.) : color, sound).
  • Contents: Diversity of vertebrate photoreceptors
  • The eye and circadian biology
  • Retinal degenerate mice
  • Mouse models & photosensitive retinal ganglion cells (pRGCs)
  • The photopigment melanopsin (OPN4) and its ablation
  • Light and pRGCs human studies (regulation of circadian rhythms).
Video
1 online resource (1 streaming video file (31 min.) : color, sound).
  • Contents: Developmental plasticity
  • Genes and environment in development
  • Adaptive developmental decisions and their betrayal if conditions change
  • Phenotypic accommodation
  • Multiple pathways
  • Myth of single gene control
  • Blueprints and programs as false metaphors
  • Cross-generational continuity of the phenotype
  • Origins of novel phenotypes including disease.
Book
1 online resource.
  • Front Cover; Endocrinology of the Heart in Health and Disease; Copyright Page; Contents; List of Contributors; Foreword; Preface; Acknowledgments; I. The Heart as an Endocrine Organ; 1 Cardiac Natriuretic Peptides; Historical Perspective; Gene Structure of Natriuretic Peptides; Atrial Natriuretic Peptide; Brain or B-type Natriuretic Peptide; C-type Natriuretic Peptide; Translation, Processing, and Storage of Cardiac Natriuretic Peptides; Atrial Natriuretic Peptide; Brain or B-Type Natriuretic Peptide; C-Type Natriuretic Peptide
  • Circulating Concentrations, Forms, and Metabolism of Natriuretic PeptidesCirculating Levels; Circulating Forms and Metabolism; Natriuretic Peptide Receptors; Circulating Natriuretic Peptide Signal Peptides; Assays Measuring Natriuretic Peptides in the Circulation; Bioactivity; Hemodynamic; Renal; Endocrine; Integrative Role in Physiology and Pathophysiology; Natriuretic Peptide as Therapeutic Agents; Enhancing Bioactivity; NEP-I; Combined ACE and NEP Inhibitors; Combined ARB and NEP Inhibitors-New Frontier in Heart Failure Therapy; Natriuretic Peptides as Biomarkers in Heart Failure
  • Natriuretic Peptides in Diagnosis of Acute Decompensated Heart FailureBNP and NT-proBNP for Community Screening for Cardiac Impairment; B-Type Natriuretic Peptides for Prognosis in Heart Failure; Marker Guided Therapy in Heart Failure; References; 2 Adrenomedullin; Introduction; Molecular Forms, Structure, and Structure-Activity Relationships of Adrenomedullin; The Adrenomedullin Receptor; Cardiac Actions of Adrenomedullin; Effect of Adrenomedullin on Cardiac Contractility; Effect of Adrenomedullin on Cardiac Structure; Pathophysiological Function of Adrenomedullin in Cardiac Disease
  • Cardiac Hypertrophy and Heart FailureAcute Myocardial Infarction; Ischemia/Reperfusion; Clinical Applications of Adrenomedullin to Cardiac Disease; Effect of Adrenomedullin Administration in Patients with Heart Failure; Clinical Application of Adrenomedullin in Acute Myocardial Infarction; Clinical Trials with Adrenomedullin; Conclusion; References; 3 Endothelin-1 as a Cardiac-Derived Autocrine, Paracrine and Intracrine Factor in Heart Health and Disease; Introduction; Cardiac Production of ET-1; Cardiac ET-1 Production in Heart Disease; Expression of Cardiac ET Receptors
  • Intracellular ET Receptors: Evidence for an Intracrine Role of ET-1 in the Cardiac CellCardiac Effects of ET-1; Mechanisms Underlying Inotropic Responses; Physiological and Pathophysiological Roles of Cardiac-Derived ET-1: General Concepts; ET-1 and the Aging Heart; Association of ET-1 With Cardiac Diseases; ET-1 in Cardiac Hypertrophy and Heart Failure; Endogenous Modulation of ET-1: Keeping ET-1 in Check?; Potential Beneficial Effects of ET-1 in Cardiac Function; Development of Clinical Strategies aimed at Targeting ET-1
Endocrinology of the Heart in Health and Disease: Integrated, Cellular, and Molecular Endocrinology of the Heart covers the traditional concepts of cardio-endocrinology, the role of the various hormone systems, both in health and disease, therapeutic implications, and other recent advances in the various fields represented. The book explores how cardiac hormones are changed in various cardiac pathologies and the recent success that has been uncovered in their therapeutic use. Additional focus is placed on how the heart responds both physiologically and pathophysiologically to a plethora of circulating hormones, reinforcing the importance of the heart as a target of numerous endocrine systems, such as the brain, renal, and adipose. Significant advances have come from basic, clinical, and translational research from a multiplicity of investigators with diverse backgrounds. The book features over 200 photomicrographs, diagrams of molecular relationships, and tables that complement and support the text. It is aimed at a wide audience, including graduate students and post-doctoral fellows in a wide array of biomedical departments and PhD programs (e.g. Pathology, Physiology, Genetics, Pharmacology, Molecular Biology, and Cell Biology) related to the endocrine and cardiovascular sciences curricula, as well as medical residents in pathology, laboratory medicine, internal medicine, and cardiology. * Develops the concept of the heart as both an endocrine organ and an endocrine target, exploring the endocrine function of the heart in both health and disease* Explains how the levels of several cardiac hormones are changed in various cardiac pathologies and how some hormones can be used therapeutically* Offers a single resource on cardio-endocrine disease which collates and curates the wide range of advances being made in the areas of molecular biology, biochemistry, physiology, and pathology.
(source: Nielsen Book Data)9780128031117 20161114
Book
1 online resource : illustrations (chiefly color)
  • * Anatomy of the eye and orbit. * Embryology and early development of the eye and adnexia. * Genetics. * Biochemistry and cell biology. * Physiology of vision and the visual system. * General and ocular pharmacology. * Immunology. * Microbiology and infection. * Pathology.* Index.
  • (source: Nielsen Book Data)9780702055546 20160618
The Eye: Basic Sciences in Practice provides highly accessible, concise coverage of all the essential basic science required by today's ophthalmologists and optometrists in training. It is also essential reading for those embarking on a career in visual and ophthalmic science, as well as an invaluable, current refresher for the range of practitioners working in this area. This new fourth edition has now been fully revised and updated in line with current curricula, key research developments and clinical best practice. It succinctly incorporates the massive strides being made by genetics and functional genomics based on the Human Genome Project, the new understanding of how the microbiome affects all aspects of immunology, the remarkable progress in imaging technology now applied to anatomy and neurophysiology, as well as exciting new molecular and other diagnostic methodologies now being used in microbiology and pathology. All this and more collectively brings a wealth of new knowledge to students and practitioners in the fields of ophthalmology and visual science. For the first time, this (print) edition also now comes with bonus access to the complete, fully searchable electronic text - including carefully selected additional information and new video content to further explain and expand on key concepts - making The Eye a more flexible, comprehensive and engaging learning package than ever before.
(source: Nielsen Book Data)9780702055546 20160618
Book
1 online resource (xxii, 585 pages) : illustrations (some color). Digital: text file; PDF.
  • Part I. Introduction to Fractal Geometry and its Applications to Neurosciences
  • The Fractal Geometry of the Brain: An Overview
  • 2. Box-Counting Fractal Analysis: A Primer for the Clinician
  • Tenets and Methods of Fractal Analysis (1/f noise)
  • 4. Tenets, Methods and Applications of Multifractal Analysis in Neurosciences
  • Part II. Fractals in Neuroanatomy and Basic Neurosciences
  • Fractals in Neuroanatomy and Basic Neurosciences: An Overview
  • Morphology and Fractal-Based Classifications of Neurons and Microglia
  • The Morphology of the Brain Neurons: Box-counting Method in Quantitative Analysis of 2D Image
  • Neuronal Fractal Dynamics
  • Does a Self-Similarity Logic Shape the Organization of the Nervous System?
  • Fractality of Cranial Sutures
  • The Fractal Geometry of the Human Brain: An Evolutionary Perspective
  • Part III. Fractals in Clinical Neurosciences
  • Fractal Analysis in Clinical Neurosciences: An Overview
  • Fractal Analysis in Neurological Diseases
  • Fractal Dimension Studies of the Brain Shape in Aging and Neurodegenerative Diseases
  • Fractal Analysis in Neurodegenerative Diseases
  • Fractal Analysis of the Cerebrovascular System Physiopathology
  • Fractal and Chaos in the Hemodynamics of Intracranial Aneurysms
  • Fractal-based Analysis of Arteriovenous Malformations (AVMs)
  • Fractals in Neuroimaging
  • Computational Fractal-Based Analysis of MR Susceptibility Weighted Imaging (SWI) in Neuro-oncology and neurotraumatology
  • Texture Estimation for Abnormal Tissue Segmentation in Brain MRI
  • Tumor Growth in the Brain: Complexity and Fractality
  • Histological Fractal-based Classification of Brain Tumors
  • Computational Fractal-based Analysis of the Brain Tumors Microvascular Networks
  • Fractal analysis of electroencephalographic time-series (EEG-signals)
  • On Multiscaling of Parkinsonian Rest Tremor Signals and Their Classification
  • Fractals and Electromyograms
  • Fractal analysis in Neuro-ophthalmology
  • Fractals in Affective and Anxiety Disorders
  • Fractal Fluency: An Intimate Relationship Between the Brain and Processing of Fractal Stimuli
  • Part IV. Computational Fractal-Based Neurosciences
  • Computational Fractal-based Neurosciences: An Overview
  • ImageJ in Computational Fractal-based Neuroscience: Pattern Extraction and Translational Research
  • Fractal Analysis in MATLAB: A Tutorial for Neuroscientists
  • Methodology to Increase the Computational Speed to Obtain the Fractal Dimension Using GPU Programming
  • Fractal Electronics as a Generic Interface to Neurons
  • Fractal Geometry meets Computational Intelligence: Future Perspectives.
Reviews the most intriguing applications of fractal analysis in neuroscience with a focus on current and future potential, limits, advantages, and disadvantages. Will bring an understanding of fractals to clinicians and researchers also if they do not have a mathematical background, and will serve as a good tool for teaching the translational applications of computational models to students and scholars of different disciplines. This comprehensive collection is organized in four parts: (1) Basics of fractal analysis; (2) Applications of fractals to the basic neurosciences; (3) Applications of fractals to the clinical neurosciences; (4) Analysis software, modeling and methodology.
Book
1 online resource (XIII, 115 p. 30 ill., 17 illus. in color.) : online resource. Digital: text file; PDF.
  • Introduction to NeuroIS.- A Primer on Neurobiology and the Brain for Information Systems Scholars.- Tools in NeuroIS Research: An Overview.- Topics in NeuroIS and a Taxonomy of Neuroscience Theories in NeuroIS.- quot--Times New Roman"--mso-ansi-language:EN-CA--mso-fareast-language:DE--mso-bi di-language: AR-SA">Establishing and Operating a NeuroIS Lab.
  • (source: Nielsen Book Data)9783662450901 20160619
This authored volume presents the fundamentals of NeuroIS, which is an emerging subfield within the Information Systems discipline that makes use of neuroscience and neurophysiological tools and knowledge to better understand the development, use, and impact of information and communication technologies. This book is an initial guide to this new research domain. The target audience primarily comprises PhD students and researchers, but the book may also be beneficial for graduate students and practitioners.
(source: Nielsen Book Data)9783662450901 20160619
Video
1 online resource (1 streaming video file (40 min.) : color, sound).
  • Contents: Complexity of the lung cancer genome
  • Molecular classification of subtypes
  • Pathways of activation in tumors
  • Intratumoral heterogeneity
  • Targetable alterations in patients
  • Emerging areas in genomics of lung cancer.
Video
1 online resource (1 streaming video file (45 min.) : color, sound).
  • Contents: Life phases and human senescent phenotypes
  • Seven major human senescence phenotypes
  • Hypertension
  • Atherosclerosis
  • Alzheimer's disease
  • Diabetes type 2
  • Muscle aging
  • Gastrointestinal cancers
  • Skin aging
  • Inflammasomes and aging.
Book
1 online resource (viii, 333 p.) Digital: text file; PDF.
  • The Mechanophysiololgy of Stress Fractures in Military Recruits.- Traumatic Brain Injury in the Military: Biomechanics and Finite Element Modelling.- Modeling Skeletal Injuries in Military Scenarios.- Biomechanics of Eye Injury in the Military.- The Biomechanical Basis for Increased Risk of Overuse Musculoskeletal Injuries in Female Soldiers.
  • (source: Nielsen Book Data)9783319330105 20160619
This book provides a state-of-the-art update, as well as perspectives on future directions of research and clinical applications in the implementation of biomechanical and biophysical experimental, theoretical and computational models which are relevant to military medicine. Such experimental and modeling efforts are helpful, on the one hand, in understanding the aetiology, pathophysiology and dynamics of injury development and on the other hand in guiding the development of better equipment and protective gear or devices that should ultimately reduce the prevalence and incidence of injuries or lessen their hazardous effects. The book is useful for military-oriented biomedical engineers and medical physicists, as well as for military physiologists and other medical specialists who are interested in the science and technology implemented in modern investigations of military related injuries.
(source: Nielsen Book Data)9783319330105 20160619
Book
1 online resource ()
  • Front Cover; Molecular Basis of Nutrition and Aging; Copyright Page; Contents; List of Contributors; Introduction to the Molecular Basis of Nutrition and Aging; Series Preface; Acknowledgments; I. Introductory Aspects on Aging and Nutrition; 1 Molecular and Cellular Basis of Aging; Introduction; Biological Principles of Aging; Occurrence, Accumulation, and Consequences of Molecular Damage; Homeodynamics and the Homeodynamic Space; Nutrition and Food for Aging Interventions; Nutritional Hormetins; Conclusions; Summary Points; References.
  • 2 Unraveling Stochastic Aging Processes in Mouse Liver: Dissecting Biological from Chronological AgeIntroduction; Pathological Parameters are only Partially Associated with Chronological Age; Intraorgan Specific Biological Phenotypes; Tissue-Specific Biological Phenotypes; Gene Expression Profiles Related to Pathological Aging Parameters; Gene Expression Profiles Correlating with Pathological Parameters are Largely Specific to the Pathological Parameters; Future Perspectives; Conclusion; References; 3 Nutrigenomics and Nutrigenetics: The Basis of Molecular Nutrition; Introduction.
  • Nutrigenetics of Omega-3 PUFA in CVDNutrigenetics of Omega-3 PUHA in Cancer; DNA Damage and Nutrients; Cellular Senescence and Nutrients; Epigenetics and Nutrients; Summary Points; References; 4 Diet and Longevity Phenotype; Introduction; Main Text; Nutrient Components and Aging Process; Crucial Role of Fatty Acid Component; Genomic Studies and Importance of Palmitoleic Acid; The Central Position of Energy Sensor Systems; The Involvement of Sympathetic System; Epigenetic Linkage of Aging and Nutrition; Summary; References; 5 Nutrition in the Elderly: General Aspects; Introduction.
  • Malnutrition in the Elderly: Definition and General AspectsCauses of Malnutrition in the Elderly; Malnutrition: Possible Correction With Supplements in the Elderly; Nutrient-Sensing Pathways; Nutrient-Gene Interaction in the Elderly (Nutrigenomic Approach); Conclusions and Perspectives; Summary Points; Acknowledgments; References; 6 Nutrition in the Hospitalized Elderly; Introduction; Pathogenesis of Malnutrition in Hospitalized Elderly; The Interaction Between Aging, Health Status, Hospital Environment, and Nutritional Status; Why Are Hospitalized Older Adults Nutritionally Vulnerable?
  • Age-Related ChangesAnorexia of Aging; Changes in the Body Composition; Taste, Smell, and Mastication Dysfunction; Gastrointestinal Changes; Medical Causes; Use of Multiple Medications; Functional and Psychosocial Factors; Hospital Environment-Related Risks; Clinical Consequences of Malnutrition in Hospital; Malnutrition Screening and Assessment in Hospitalized Elderly; Malnutrition Screening as a Part from Comprehensive Geriatric Assessment (CGA); Comprehensive Nutritional Assessment; Nutritional History; Physical and Clinical Assessment; Anthropometry; The Biochemical Investigations.
Video
1 online resource (1 streaming video file (39 min.) : color, sound).
  • Contents: Neuropathology of amyotrophic lateral sclerosis (ALS)
  • Neuroinflammation in human ALS
  • Neuroinflammation in ALS mouse model
  • Role of microglia in ALS
  • Role of T cells in ALS
  • TREM2
  • Progranulin.
Book
1 online resource.
  • 1- Models for studying Organogenetic gene networks in the 21st century James Castelli-Gair Hombria and Paola Bovolenta 2- Organogenesis of the C. elegans vulva and control of cell fusion Nathan Weinstein and Benjamin Podbilewicz 3- Advances in understanding the generation and specification of unique neuronal sub-types from Drosophila neuropeptidergic neurons Stefan Thor and Douglas W. Allan 4- Fast and Furious 800. The retinal determination gene network in Drosophila. Fernando Casares and Isabel Almudi 5- Genetic control of salivary gland tubulogenesis in Drosophila Clara Sidor and Katja Roper 6- Organogenesis of the Drosophila respiratory system Rajprasad Loganathan, Yim Ling Cheng, Deborah J. Andrew 7- Organogenesis of the zebrafish kidney George Chang, Richard W. Naylor, and Alan J. Davidson 8- Morphogenetic mechanisms of inner ear development Berta Alsinaand Andrea Streit 9- Vertebrate eye gene regulatory networks Juan R. Martinez-Morales 10- Vertebrate eye evolution Juan R. Martinez-Morales and Annamaria Locascio 11- Principles of early vertebrate forebrain formation Florencia Cavodeassi, Tania Moreno-Marmol, Maria Hernandez-Bejarano and Paola Bovolenta 12- Control of organoganesis by Hox genes James Castelli-Gair Hombria, Carlos Sanchez-Higeras and Ernesto Sanchez-Herrero Index.
  • (source: Nielsen Book Data)9783319427652 20161010
All animals, including humans, derive from a single cell, which possesses all the genetic instructions needed to define how the animal will look like. However, during development, the millions of cells that derive from the zygote will only select part of this genetic information to give rise to the various organs of the body. The coordination of different cell behaviours during development results in the formation of specialized tissues and organs giving rise to highly adapted animals. This book provides an overview of how this diversification is achieved during organ formation and how it may have evolved. Conserved cellular processes are presented using examples from selected vertebrate and invertebrate species that illustrate how developmental biologists are solving the complex puzzle of organ formation. This volume is aimed to students, researchers and medical doctors alike who want to find a simple but rigorous introduction on how gene networks control organ formation."div>.
(source: Nielsen Book Data)9783319427652 20161010
Book
1 online resource.
  • Dedication; Preface; Contents; Contributors; 1: Historical Background of Pancreatic Islet Isolation; 1.1 The History of Diabetes Mellitus; 1.1.1 Discovery of Insulin; 1.2 The History of Islet Isolation; 1.3 Concluding Remarks; References; 2: The Different Faces of the Pancreatic Islet; 2.1 Introduction; 2.1.1 Cytoarchitecture; 2.1.2 Vasculature; 2.1.3 Paracrine Signaling; 2.1.4 Innervation; 2.2 Summary; References; 3: Isolation of Mouse Pancreatic Islets of Langerhans; 3.1 Introduction; 3.2 Pancreas Distention and Dissection; 3.2.1 General Considerations About the Procedure
  • 3.2.2 Enzyme Administration3.3 Pancreas Digestion; 3.3.1 General Considerations About the Procedure; 3.3.2 Selection of the Enzyme; 3.3.3 Duration of Digestion; 3.4 Islet Purification; 3.4.1 General Considerations About the Procedure; 3.4.2 Density Gradient Purification; 3.4.3 Other Purification Methods; 3.5 Islet Culture; 3.5.1 General Considerations About the Procedure; 3.6 Concluding Remarks; References; 4: Pancreatic Islets: Methods for Isolation and Purification of Juvenile and Adult Pig Islets; 4.1 Introduction; 4.2 Pig Donor Variables; 4.2.1 Pig Strain
  • 4.2.2 Porcine Donor Age4.3 Pig Pancreas Procurement; 4.3.1 Intraductal Pancreas Perfusion Technique; 4.3.2 Intraductal Pancreas Perfusion Buffer; 4.4 Pig Pancreas Digestion; 4.4.1 Enzyme Blend Selection; 4.4.2 Dissociation; 4.5 Porcine Islet Purification; 4.5.1 Determinants of Intrinsic Pig Pancreas Density; 4.5.2 Reversing Cell Swelling; 4.5.3 Selection of Density Gradient Media; 4.5.4 Density Gradient Centrifugation Technique; 4.5.5 Isokinetic Islet Purification; 4.6 Porcine Islet Culture; 4.6.1 Selecting the Most Suitable Culture Medium; 4.6.2 Glucose Concentration
  • 4.6.3 Serum Content4.6.4 Glutamine Supplementation; 4.6.5 Culture Temperature; 4.7 Summary; References; 5: Isolation of Pancreatic Islets from Nonhuman Primates; 5.1 Introduction; 5.2 Description of the Procedure and Key Aspects; 5.2.1 Laboratory Set Up; 5.2.1.1 Procedures Prior to the Day of Islet Isolation; 5.2.1.2 Procedures on the Day of Islet Isolation; Solutions and Instruments; Preparation of Digestive Enzymes; Pancreas Procurement; Collagenase Digestion and Dilution; Concentration and Purification of Islets; Determination of Islet Yield and Purity
  • Islet Assessment in NHP IsletsIn Vitro Tests; In Vivo Test; 5.3 Conclusions; References; 6: Necessities for a Clinical Islet Program; 6.1 Introduction; 6.2 The Islet Processing Facility; 6.3 Clinical Islet Isolation Outcomes; 6.4 The Donor Organ; 6.4.1 Effect of Donor Age; 6.4.2 Effect of BMI; 6.4.3 Effect of Cold Ischaemic Time (CIT); 6.5 Organ Retrieval; 6.6 Pancreas Preservation; 6.6.1 The Two-Layer Method; 6.6.2 Machine Perfusion; 6.7 The Pancreas; 6.8 Concluding Remarks; References; 7: Clinical Islet Isolation