Book
1 online resource.
  • Employing 'second generation' matrices.- (MA)LDI MS mass spectrometry imaging at high specificity and sensitivity.- Techniques for fingermark analysis using MALDI MS - a practical overview.- Whole/Intact Cell MALDI MS Biotyping in Mammalian Cell Analyis.- MALDI biotyping for microorganism identification in clinical microbiology.- Future applications of MALDI-TOF MS in microbiology.- MALDESI: Fundamentals, direct analysis, and MS imaging.- Microprobe MS Imaging of Live Tissues, Cells, and Bacterial Colonies using LAESI.- Efficient production of multiply charged MALDI ions.- Food Authentication by MALDI MS - MALDI-TOF MS Analysis of Fish Species.- Quantitative MALDI MS using Ionic Liquid Matrices.- Disease profiling by MALDI MS analysis of biofluids.- Ionic Liquids and other liquid matrices for sensitive MALDI MS analysis.- Coupling liquid MALDI MS to liquid chromatography.
  • (source: Nielsen Book Data)
This book covers the state-of-the-art of modern MALDI (matrix-assisted laser desorption/ionization) and its applications. New applications and improvements in the MALDI field such as biotyping, clinical diagnosis, forensic imaging, and ESI-like ion production are covered in detail. Additional topics include MS imaging, biotyping/speciation and large-scale, high-speed MS sample profiling, new methods based on MALDI or MALDI-like sample preparations, and the advantages of ESI to MALDI MS analysis. This is an ideal book for graduate students and researchers in the field of bioanalytical sciences. This book also: * Showcases new techniques and applications in MALDI MS * Demonstrates how MALDI is preferable to ESI (electrospray ionization) * Illustrates the pros and cons associated with biomarker discovery studies in clinical proteomics and the various application areas, such as cancer proteomics.
(source: Nielsen Book Data)
Book
1 online resource (viii, 336 pages) : illustrations (some color)
In the last quarter century, advances in mass spectrometry (MS) have been at the forefront of efforts to map complex biological systems including the human metabolome, proteome, and microbiome. All of these developments have allowed MS to become a well-established molecular level technology for microbial characterization. MS has demonstrated its considerable advantage as a rapid, accurate, and cost-effective method for microbial identification, compared to conventional phenotypic techniques. In the last several years, applications of MS for microbial characterization in research, clinical microbiology, counter-bioterrorism, food safety, and environmental monitoring have been documented in thousands of publications. Regulatory bodies in Europe, the US, and elsewhere have approved MS-based assays for infectious disease diagnostics. As of mid-2015, more than 3300 commercial MS systems for microbial identification have been deployed worldwide in hospitals and clinical labs. While previous work has covered broader approaches in using MS to characterize microorganisms at the species level or above, this book focuses on strain-level and subtyping applications. In thirteen individual chapters, innovators, leaders and practitioners in the field from around the world have contributed to a comprehensive overview of current and next-generation approaches for MS-based microbial characterization at the subspecies and strain levels. Chapters include up-to-date reference lists as well as web-links to databases, recommended software, and other useful tools. The emergence of new, antibiotic-resistant strains of human or animal pathogens is of extraordinary concern not only to the scientific and medical communities, but to the general public as well. Developments of novel MS-based assays for rapid identification of strains of antibiotic-resistant microorganisms are reviewed in the book as well. Microbiologists, bioanalytical scientists, infectious disease specialists, clinical laboratory and public health practitioners as well as researchers in universities, hospitals, government labs, and the pharmaceutical and biotechnology industries will find this book to be a timely and valuable resource.
Book
online resource (xv, 333 pages) : illustrations (some color)
  • Mass spectrometry in clinical laboratory : applications in biomolecular analysis / Uttam Garg and Yan Victoria Zhang
  • Quantification of free carnitine and acylcarnitines in plasma or serum using HPLC/MS/MS / David Scott, Bryce Heese, and Uttam Garg
  • Quantification of arginine and its methylated derivatives in plasma by high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) / Faye B. Vicente ... [et al.]
  • Quantitation of albumin in urine by liquid chromatography tandem mass spectrometry / Hemamalini Ketha and Ravinder J. Singh
  • Quantitation of aldosterone in serum or plasma using liquid chromatography-tandem mass spectrometry (LC- MS/MS) / J. Grace Van Der Gugten and Daniel T. Holmes
  • Quantification of five clinically important amino acids by HPLC-Triple TOF 5600 based on pre-column double derivatization method / Shuang Deng, David Scott, and Uttam Garg
  • Sensitive, simple, and robust nano-liquid chromatography-mass spectrometry method for amyloid protein subtyping / Drew Payto, Courtney Heideloff, and Sihe Wang
  • Quantitation of ubiquinone (coenzyme Q10) in serum/plasma using liquid chromatography electrospray tandem mass spectrometry (ESI-LC-MS/MS) / Richard E. Mathieu Jr. and Catherine P. Riley
  • Quantitative analysis of salivary cortisol using LC-MS/MS / Yan Victoria Zhang
  • Quantification of dihydroxyacetone phosphate (DHAP) in human red blood cells by HPLC-TripleTOF 5600 mass spectrometer / Shuang Deng ... [et al.]
  • Simultaneous quantitation of estradiol and sstrone in serum using liquid chromatography mass spectrometry / Catherine P. Riley, Richard E. Mathieu Jr., and Carmen Wiley
  • Direct measurement of free estradiol in human serum and plasma by equilibrium dialysis-liquid chromatography-tandem mass spectrometry / Julie A. Ray ... [et al.]
  • Quantification of γ-aminobutyric acid in cerebrospinal fluid using liquid chromatography-electrospray tandem mass spectrometry / Erland Arning and Teodoro Bottiglieri
  • Quantitation of insulin analogues in serum using immunoaffinity extraction, liquid chromatography, and tandem mass spectrometry / J. Grace Van Der Gugten, Sophia Wong, and Daniel T. Holmes
  • Quantitation of insulin-like growth factor 1 in serum by liquid chromatography high resolution accurate-mass mass spectrometry / Hemamalini Ketha and Ravinder J. Singh
  • Quantitation of free metanephrines in plasma by liquid chromatography-tandem mass spectrometry / Courtney Heideloff, Drew Payto, and Sihe Wang
  • Quantification of metanephrine and normetanephrine in urine using liquid chromatography-tandem mass spectrometry / Jessica Gabler and Sihe Wang
  • High-throughput analysis of methylmalonic acid in serum, plasma, and urine by LC-MS/MS. Method for analyzing isomers without chromatographic separation / Mark M. Kushnir ... [et al.]
  • Quantitation of 5-methyltetrahydrofolate in cerebrospinal fluid using liquid chromatography-electrospray tandem mass spectrometry / Erland Arning and Teodoro Bottiglieri
  • Quantitative organic acids in urine by two dimensional gas chromatography-time of flight mass spectrometry (GCxGC-TOFMS) / Lawrence Sweetman, Paula Ashcraft, and Jeanna Bennett-Firmin
  • High sensitivity measurement of pancreatic polypeptide and its variant in serum and plasma by LC-MS/MS / Hernando Escobar ... [et al.]
  • Quantitation of parathyroid hormone in serum or plasma by liquid chromatography-tandem mass spectrometry / Hemamalini Ketha and Ravinder J. Singh
  • Determination of phenylalanine and tyrosine by high performance liquid chromatography-tandem mass spectrometry / Judy Peat and Uttam Garg
  • Urine purine metabolite determination by UPLC-tandem mass spectrometry / Qin Sun
  • Urine pyrimidine metabolite determination by HPLC tandem mass spectrometry / Qin Sun
  • Quantitation of plasma renin activity in plasma using liquid chromatography-tandem mass spectrometry (LC- MS/MS) / J. Grace Van Der Gugten and Daniel T. Holmes
  • Quantitation of S-adenosylmethionine and S-adenosylhomocysteine in plasma using liquid chromatography electrospray tandem mass spectrometry / Erland Arning and Teodoro Bottiglieri
  • Simple, high-throughput method for analysis of ceramide, glucosylceramide, and ceramide trihexoside in dried blood spots by LC/MS/MS / Wei-Lien Chuang, Joshua Pacheco, and Kate Zhang
  • Quantification of dehydroepiandrosterone, 11-Deoxycortisol, 17-Hydroxyprogesterone, and testosterone by liquid chromatography-tandem mass spectrometry (LC/MS/MS) / Ada Munar, Clint Frazee, and Uttam Garg
  • Urinary succinylacetone analysis by gas chromatography-mass spectrometry (GC-MS) / Hongjie Chen and Chunli Yu
  • Quantification of 1,25-Dihydroxyvitamin D2 and D3 in serum using liquid chromatography-tandem mass spectrometry / Jonathon Mahlow, Dustin R. Bunch, and Sihe Wang
  • High-throughput serum 25-hydroxy vitamin D testing with automated sample preparation / Judy Stone
  • Quantitation of 25-OH-Vitamin-D2 and 25-OH-Vitamin-D3 in urine using LC-MS/MS / Dean C. Carlow, Ryan C. Schofield, and Michelle Denburg.
Medical Library (Lane)
Book
xv, 333 pages : illustrations (some color) ; 26 cm.
  • Mass spectrometry in clinical laboratory : applications in biomolecular analysis / Uttam Garg and Yan Victoria Zhang
  • Quantification of free carnitine and acylcarnitines in plasma or serum using HPLC/MS/MS / David Scott, Bryce Heese, and Uttam Garg
  • Quantification of arginine and its methylated derivatives in plasma by high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) / Faye B. Vicente [and others]
  • Quantitation of albumin in urine by liquid chromatography tandem mass spectrometry / Hemamalini Ketha and Ravinder J. Singh
  • Quantitation of aldosterone in serum or plasma using liquid chromatography-tandem mass spectrometry (LC- MS/MS) / J. Grace Van Der Gugten and Daniel T. Holmes
  • Quantification of five clinically important amino acids by HPLC-Triple TOF 5600 based on pre-column double derivatization method / Shuang Deng, David Scott, and Uttam Garg
  • Sensitive, simple, and robust nano-liquid chromatography-mass spectrometry method for amyloid protein subtyping / Drew Payto, Courtney Heideloff, and Sihe Wang
  • Quantitation of ubiquinone (coenzyme Q10) in serum/plasma using liquid chromatography electrospray tandem mass spectrometry (ESI-LC-MS/MS) / Richard E. Mathieu Jr. and Catherine P. Riley
  • Quantitative analysis of salivary cortisol using LC-MS/MS / Yan Victoria Zhang
  • Quantification of dihydroxyacetone phosphate (DHAP) in human red blood cells by HPLC-TripleTOF 5600 mass spectrometer / Shuang Deng [and others]
  • Simultaneous quantitation of estradiol and sstrone in serum using liquid chromatography mass spectrometry / Catherine P. Riley, Richard E. Mathieu Jr., and Carmen Wiley
  • Direct measurement of free estradiol in human serum and plasma by equilibrium dialysis-liquid chromatography-tandem mass spectrometry / Julie A. Ray [and others]
  • Quantification of γ-aminobutyric acid in cerebrospinal fluid using liquid chromatography-electrospray tandem mass spectrometry / Erland Arning and Teodoro Bottiglieri
  • Quantitation of insulin analogues in serum using immunoaffinity extraction, liquid chromatography, and tandem mass spectrometry / J. Grace Van Der Gugten, Sophia Wong, and Daniel T. Holmes
  • Quantitation of insulin-like growth factor 1 in serum by liquid chromatography high resolution accurate-mass mass spectrometry / Hemamalini Ketha and Ravinder J. Singh
  • Quantitation of free metanephrines in plasma by liquid chromatography-tandem mass spectrometry / Courtney Heideloff, Drew Payto, and Sihe Wang
  • Quantification of metanephrine and normetanephrine in urine using liquid chromatography-tandem mass spectrometry / Jessica Gabler and Sihe Wang
  • High-throughput analysis of methylmalonic acid in serum, plasma, and urine by LC-MS/MS. Method for analyzing isomers without chromatographic separation / Mark M. Kushnir [and others]
  • Quantitation of 5-methyltetrahydrofolate in cerebrospinal fluid using liquid chromatography-electrospray tandem mass spectrometry / Erland Arning and Teodoro Bottiglieri
  • Quantitative organic acids in urine by two dimensional gas chromatography-time of flight mass spectrometry (GCxGC-TOFMS) / Lawrence Sweetman, Paula Ashcraft, and Jeanna Bennett-Firmin
  • High sensitivity measurement of pancreatic polypeptide and its variant in serum and plasma by LC-MS/MS / Hernando Escobar [and others]
  • Quantitation of parathyroid hormone in serum or plasma by liquid chromatography-tandem mass spectrometry / Hemamalini Ketha and Ravinder J. Singh
  • Determination of phenylalanine and tyrosine by high performance liquid chromatography-tandem mass spectrometry / Judy Peat and Uttam Garg
  • Urine purine metabolite determination by UPLC-tandem mass spectrometry / Qin Sun
  • Urine pyrimidine metabolite determination by HPLC tandem mass spectrometry / Qin Sun
  • Quantitation of plasma renin activity in plasma using liquid chromatography-tandem mass spectrometry (LC- MS/MS) / J. Grace Van Der Gugten and Daniel T. Holmes
  • Quantitation of S-adenosylmethionine and S-adenosylhomocysteine in plasma using liquid chromatography electrospray tandem mass spectrometry / Erland Arning and Teodoro Bottiglieri
  • Simple, high-throughput method for analysis of ceramide, glucosylceramide, and ceramide trihexoside in dried blood spots by LC/MS/MS / Wei-Lien Chuang, Joshua Pacheco, and Kate Zhang
  • Quantification of dehydroepiandrosterone, 11-Deoxycortisol, 17-Hydroxyprogesterone, and testosterone by liquid chromatography-tandem mass spectrometry (LC/MS/MS) / Ada Munar, Clint Frazee, and Uttam Garg
  • Urinary succinylacetone analysis by gas chromatography-mass spectrometry (GC-MS) / Hongjie Chen and Chunli Yu
  • Quantification of 1,25-Dihydroxyvitamin D2 and D3 in serum using liquid chromatography-tandem mass spectrometry / Jonathon Mahlow, Dustin R. Bunch, and Sihe Wang
  • High-throughput serum 25-hydroxy vitamin D testing with automated sample preparation / Judy Stone
  • Quantitation of 25-OH-Vitamin-D2 and 25-OH-Vitamin-D3 in urine using LC-MS/MS / Dean C. Carlow, Ryan C. Schofield, and Michelle Denburg.
This volume provides stepwise instructions for the analysis of numerous clinically important analytes by mass spectrometry. Mass spectrometry offers clinical laboratory scientists a number of advantages including increased sensitivity and specificity, multiple component analysis, and no need for specialized reagents. The techniques described are a must for the measurement of many clinically relevant analytes in the fields of drug analysis, endocrinology, and inborn errors of metabolism. Each chapter provides a brief introduction about a specified analyte, followed by detailed instructions on the analytical protocol. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and practical, Clinical Applications of Mass Spectrometry in Biomolecular Analysis: Methods and Protocols is a great resource for clinical laboratory scientists who are already using or thinking of bringing mass spectrometry to their laboratories.
(source: Nielsen Book Data)
Biology Library (Falconer)
Book
xv, 272 pages : illustrations (some color) ; 26 cm.
  • Mass spectrometry in clinical laboratory : applications in therapeutic drug monitoring and toxicology / Uttam Garg and Yan Victoria Zhang
  • Quantitation of flecainide, mexiletine, propafenone, and amiodarone in serum or plasma using liquid chromatography-tandem mass spectrometry (LC-MS/MS) / Matthew H. Slawson and Kamisha L. Johnson-Davis
  • Quantitation of the oral anticoagulants dabigatran, rivaroxaban, apixaban, and warfarin in plasma using ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) / Jaime H. Noguez and James C. Ritchie
  • Simultaneous quantitation of lamotrigine, levetiracetam, 10-Hydroxycarbazepine, topiramate, and zonisamide in serum using HPLC-MS/MS / Dean C. Carlow, Heng Shi, and Ryan C. Schofield
  • Quantification of the triazole antifungal compounds voriconazole and posaconazole in human serum or plasma using liquid chromatography electrospray tandem mass spectrometry (HPLC-ESI-MS/MS) / Alejandro R. Molinelli and Charles H. Rose IV
  • Quantitation of haloperidol, fluphenazine, perphenazine, and thiothixene in serum or plasma using liquid chromatography-tandem mass spectrometry (LC-MS/MS) / Matthew H. Slawson and Kamisha L. Johnson-Davis
  • Quantitation of total buprenorphine and norbuprenorphine in meconium by LC-MS/MS / Stephanie J. Marin and Gwendolyn A. McMillin
  • Quantitation of buprenorphine, norbuprenorphine, buprenorphine glucuronide, norbuprenorphine glucuronide, and naloxone in urine by LC-MS/MS / Stephanie J. Marin and Gwendolyn A. McMillin
  • Simple liquid chromatography tandem mass spectrometry method for quantitation of plasma busulfan / Shuang Deng [and others]
  • High-throughput quantitation of busulfan in plasma using ultrafast solid-phase extraction tandem mass spectrometry (SPE-MS/MS) / Loralie J. Langman [and others]
  • Quantification of 11-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in meconium using gas chromatography/mass spectrometry (GC/MS) / Judy Peat [and others]
  • Quantitation of carisoprodol and meprobamate in urine and plasma using liquid chromatography-tandem mass spectrometry (LC-MS/MS) / Matthew H. Slawson and Kamisha L. Johnson-Davis
  • Cetirizine quantification by high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) / Ada Munar [and others]
  • Quantification of docetaxel in serum using turbulent flow liquid chromatography electrospray tandem mass spectrometry (TFC-HPLC-ESI-MS/MS) / Christopher A. Crutchfield, Mark A. Marzinke, and William A. Clarke
  • Comprehensive urine drug screen by gas chromatography/mass spectrometry (GC/MS) / Bheemraj Ramoo [and others]
  • Broad-spectrum drug screening using liquid chromatography-hybrid triple-quadrupole linear ion trap mass spectrometry / Judy Stone
  • High-resolution mass spectrometry for untargeted drug screening / Alan H.B. Wu and Jennifer Colby
  • Quantitation of ethyl glucuronide and ethyl sulfate in urine using liquid chromatography-tandem mass spectrometry (LC-MS/MS) / Matthew H. Slawson and Kamisha L. Johnson-Davis
  • Quantification of hydroxychloroquine in blood using turbulent flow liquid chromatography-tandem mass spectrometry (TFLC-MS/MS) / Allison B. Chambliss, Anna K. Füzéry, and William A. Clarke
  • Quantification of iohexol in serum by high-performance liquid chromatography-tandem mass spectrometry (LC- MS/MS) / Faye B. Vicente [and others]
  • Quantitation of teriflunomide in human serum/plasma across a 40,000-fold concentration range by LC/MS/MS / Geoffrey S. Rule, Alan L. Rockwood, and Kamisha L. Johnson-Davis
  • Determination of menthol in plasma and urine by gas chromatography/mass spectrometry (GC/MS) / Judy Peat [and others]
  • Development of an assay for methotrexate and Its metabolites 7-hydroxy methotrexate and DAMPA in serum by LC-MS/MS / Ryan C. Schofield [and others]
  • Quantitative, multidrug pain medication testing by liquid chromatography : tandem mass spectrometry (LC-MS/MS) / Geza S. Bodor
  • Quantification of free phenytoin by liquid chromatography tandem mass spectrometry (LC/MS/MS) / Judy Peat, Clint Frazee, and Uttam Garg
  • Detection of stimulants and narcotics by liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometry for sports doping control / Brian D. Ahrens, Yulia Kucherova, and Anthony W. Butch
  • Quantification of tricyclic antidepressants in serum using liquid chromatography electrospray tandem mass spectrometry (HPLC-ESI-MS/MS) / Christopher A. Crutchfield, Autumn R. Breaud, and William A. Clarke.
This volume describes methods and protocols for a number of drugs and toxins in a stepwise manner. Chapters in the book cover a wide array of topics such as: quantitation of Flecainide, Mexiletine, Propafenone, and Amiodarone in Serum or Plasma; quantitation of total Buprenorphine and Norbuprenorphine in Meconium; quantitation or Carisoprodol and Meprobamate in Urine; and quantitation of Tricyclic Antidepressants in Serum. Each chapter contains a brief introduction to the topic, clinical utility of the analyte(s), and useful notes to help laboratorians easily reproduce the protocols discussed. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Clinical Applications of Mass Spectrometry in Drug Analysis: Methods and Protocols, is a great resource for laboratorians who are already using mass spectrometry or thinking of introducing this technology to their laboratories.
(source: Nielsen Book Data)
Biology Library (Falconer)
Book
xxiii, 350 pages, 46 unnumbered pages of plates : illustrations (some color) ; 25 cm
Biology Library (Falconer)
Book
xii, 298 p. : ill. ; 25 cm
Introduction to Protein Mass Spectrometry provides a comprehensive overview of this increasingly important, yet complex, analytical technique. Unlike many other methods which automatically yield an absolutely unique protein name as output, protein mass spectrometry generally requires a deduction of protein identity from determination of peptide fragmentation products. This book enables readers to both understand, and appreciate, how determinations about protein identity from mass spectrometric data are made. Coverage begins with the technical basics, including preparations, instruments, and spectrometric analysis of peptides and proteins, before exploring applied use in biological applications, bioinformatics, database, and software resources. Citing the most recent and relevant work in the field of biological mass spectrometry, the book is written for researchers and scientists new to the field, but is also an ideal resource for those hoping to hone their analytical abilities. * Offers introductory information for scientists and researchers new to the field, as well as advanced insight into the critical assessment of computer-analyzed mass spectrometric results and their current limitations* Provides examples of commonly-used MS instruments from Bruker, Applied Biosystems, JEOL, Thermo Scientific/Thermo Fisher Scientific, IU, and Waters* Includes biological applications and exploration of analytical tools and databases for bioinformatics.
(source: Nielsen Book Data)
Biology Library (Falconer)
Book
xiii, 306 pages : illustrations (some color) ; 26 cm
  • Increased depth and breadth of plasma protein quantitation via two-dimensional liquid chromatography/multiple reaction monitoring-mass spectrometry with labeled peptide standards / Andrew J. Percy ... [et al.]
  • Quantitative analysis of the Sirt5-regulated lysine succinylation proteome in mammalian cells / Yue Chen
  • Determining the composition and stability of protein complexes using an integrated label-free and stable isotope labeling strategy / Todd M. Greco, Amanda J. Guise, and Ileana M. Cristea
  • Label-free quantitation for clinical proteomics / Robert Moulder, Young Ah Goo, and David R. Goodlett
  • Proteogenomic methods to improve genome annotation / Keshava K. Datta, Anil K. Madugundu, and Harsha Gowda
  • Mass spectrometry-based quantitative O-GlcNAcomic analysis / Junfeng Ma and Gerald W. Hart
  • Isolating and quantifying plasma HDL proteins by sequential density gradient ultracentrifugation and targeted proteomics / Clark M. Henderson, Tomas Vaisar, and Andrew N. Hoofnagle
  • Method for label-free, differential top-down proteomics / Ioanna Ntai ... [et al.]
  • Multiplexed immunoaffinity enrichment of peptides with anti-peptide antibodies and quantification by stable isotope dilution multiple reaction monitoring mass spectrometry / Eric Kuhn and Steven A. Carr
  • High-throughput quantitative proteomics enabled by mass defect-based 12-plex DiLeu isobaric tags / Dustin C. Frost and Lingjun Li
  • Isotopic N, N-Dimethyl leucine (iDiLeu) for absolute quantification of peptides using a standard curve approach / Tyler Greer and Lingjun Li
  • Selecting optimal peptides for targeted proteomic experiments in human plasma using in vitro synthesized proteins as analytical standards / James G. Bollinger ... [et al.]
  • Using the CPTAC assay portal to identify and implement highly characterized targeted proteomics assays / Jeffrey R. Whiteaker ... [et al.]
  • Large-scale and deep quantitative proteome profiling using isobaric labeling coupled with two-dimensional LC-MS/MS / Marina A. Gritsenko ... [et al.]
  • Multiple and selective reaction monitoring using triple quadrupole mass spectrometer : preclinical large cohort analysis / Qin Fu ... [et al.]
  • Methods for SWATH™ : data independent acquisition on tripleTOF mass spectrometers / Ronald J. Holewinski ... [et al.]
  • Measurement of phosphorylated peptides with absolute quantification / Raven J. Reddy ... [et al.]
  • Proteomic analysis of protein turnover by metabolic whole rodent pulse-chase isotopic labeling and shotgun mass spectrometry analysis / Jeffrey N. Savas, Sung Kyu Park, and John R. Yates, III.
Biology Library (Falconer)
Book
1 online resource.
  • An Introduction to Ambient Ionization Mass Spectrometry-- Direct Analysis in real time (DART(R))-- Ionization Mechanisms of Direct Analysis in Real Time (DART)-- Atmospheric Samples Analysis Probe (ASAP) Mass Spectrometry-- Ambient Analysis by Thermal Desorption Atmospheric Pressure Photoionization-- Low Temperature Plasma Probe-- Flowing Atmospheric Pressure Afterglow (FAPA), the Plasma-based Source for your ADI-MS needs-- Spray Desorption Collection and DESI Mechanisms-- Easy Ambient Sonic-Spray Ionization-- Secondary Electrospray Ionization-- Probe Electrospray Ionization-- Desorption Electrospray Mass Spectrometry-- Surface Acoustic Wave Nebulization-- Laser Ablation Electrospray Ionization Mass Spectrometry: Mechanisms, Configurations and Imaging Applications-- Electrospray Laser Desorption Ionization Mass Spectrometry-- Paper Spray-- Inlet and Vacuum Ionization from Ambient Conditions-- Enabling Automated Sample Analysis by Direct Analysis in Real Time (DART) Mass Spectrometry-- Laser Ablation Electrospray Ionization Mass Spectrometry (LAESI(R)-MS): Ambient Ionization Technology for 2D and 3D Molecular Imaging-- Liquid Extraction Surface Analysis Mass Spectrometry (LESA MS): Combining Liquid Extraction, Surface Profiling and Ambient Ionization Mass Spectrometry in One Novel Analysis Technique-- Subject Index.
  • (source: Nielsen Book Data)
Ambient ionization has emerged as one of the hottest and fastest growing topics in mass spectrometry enabling sample analysis with minimal sample preparation. Introducing the subject and explaining the basic concepts and terminology, this book will provide a comprehensive, unique treatise devoted to the subject. Written by acknowledged experts, there are full descriptions on how new ionization techniques work, with an overview of their strengths, weaknesses and applications. This title will bring the reader right up to date, with both applications and theory, and will be suitable as a tutorial text for those starting in the field from a variety of disciplines.
(source: Nielsen Book Data)
Book
xxii, 378 pages : illustrations (some color) ; 25 cm
  • List of Contributors XIII Preface XVII Abbreviations XIX 1 Introduction to Mass Spectrometry, a Tutorial 1 Wilfried M.A. Niessen and David Falck 1.1 Introduction 1 1.2 Figures of Merit 1 1.2.1 Introduction 1 1.2.2 Resolution 2 1.2.3 Mass Accuracy 4 1.2.4 General Data Acquisition in MS 5 1.3 Analyte Ionization 6 1.3.1 Introduction 6 1.3.2 Electrospray Ionization 8 1.3.3 Matrix-Assisted Laser Desorption Ionization 10 1.3.4 Other Ionization Methods 10 1.3.5 Solvent and Sample Compatibility Issues 11 1.4 Mass Analyzer Building Blocks 12 1.4.1 Introduction 12 1.4.2 Quadrupole Mass Analyzer 13 1.4.3 Ion-Trap Mass Analyzer 13 1.4.4 Time-of-Flight Mass Analyzer 15 1.4.5 Fourier Transform Ion Cyclotron Resonance Mass Spectrometer 16 1.4.6 Orbitrap Mass Analyzer 17 1.4.7 Ion Detection 18 1.5 Tandem Mass Spectrometry 18 1.5.1 Introduction: Tandem-in-Time and Tandem-in-Space 18 1.5.2 Ion Dissociation Techniques 20 1.5.3 Tandem Quadrupole MS MS Instruments 21 1.5.4 Ion-Trap MSn Instruments 23 1.5.5 Tandem TOF (TOF TOF) Instruments 23 1.5.6 Hybrid Instruments (Q TOF, Q LIT, IT TOF) 24 1.5.7 MS MS and MSn in FT-ICR-MS 26 1.5.8 Orbitrap-Based Hybrid Systems 27 1.5.9 Ion-Mobility Spectrometry Mass Spectrometry 28 1.6 Data Interpretation and Analytical Strategies 30 1.6.1 Data Acquisition in MS Revisited 30 1.6.2 Quantitative Bioanalysis and Residue Analysis 31 1.6.3 Identification of Small-Molecule Known Unknowns 32 1.6.4 Identification of Drug Metabolites 33 1.6.5 Protein Molecular Weight Determination 37 1.6.6 Peptide Fragmentation and Sequencing 38 1.6.7 General Proteomics Strategies: Top-Down, Middle-Down, Bottom-Up 39 1.7 Conclusion and Perspectives 43 References 43 Part I Direct MS Based Affinity Techniques 55 2 Studying Protein Protein Interactions by Combining Native Mass Spectrometry and Chemical Cross-Linking 57 Michal Sharon and Andrea Sinz 2.1 Introduction 57 2.2 Protein Analysis by Mass Spectrometry 58 2.3 Native MS 59 2.3.1 Instrumentation for High-mass ion Detection 60 2.3.2 Defining the Exact Mass of the Composing Subunits 60 2.3.3 Analyzing the Intact Complex 61 2.4 Chemical Cross-linking MS 64 2.4.1 Types of Cross-linkers 64 2.4.2 MS/MS Cleavable Cross-linkers 66 2.4.3 Data Analysis 68 2.5 Value of Combining NativeMS with Chemical Cross-linkingMS 68 2.6 Regulating the Giant 69 2.7 Capturing Transient Interactions 70 2.8 An Integrative Approach for Obtaining Low-Resolution Structures of Native Protein Complexes 72 2.9 Future Directions 73 References 74 3 Native Mass Spectrometry Approaches Using Ion Mobility-Mass Spectrometry 81 Frederik Lermyte, Esther Marie Martin, Albert Konijnenberg, Filip Lemiere, and Frank Sobott 3.1 Introduction 81 3.2 Sample Preparation 82 3.3 Electrospray Ionization 84 3.4 Mass Analyzers and Tandem MS Approaches 88 3.5 Ion Mobility 90 3.6 Data Processing 95 3.7 Challenges and Future Perspectives 98 References 102 Part II LC MS Based with Indirect Assays 109 4 Methodologies for Effect-Directed Analysis: Environmental Applications, Food Analysis, and Drug Discovery 111 Willem Jonker, Marja Lamoree, Corine J. Houtman, and Jeroen Kool 4.1 Introduction 111 4.2 Principle of Traditional Effect-Directed Analysis 113 4.3 Sample Preparation 113 4.3.1 Environmental Analysis 113 4.3.2 Food Analysis 121 4.3.3 Drug Discovery 124 4.4 Fractionation for Bioassay Testing 126 4.4.1 Environmental Analysis 126 4.4.2 Food Analysis 130 4.4.3 Drug Discovery 131 4.5 Miscellaneous Approaches 133 4.6 Bioassay Testing 136 4.6.1 Environmental Analysis 136 4.6.2 Food Analysis 140 4.6.3 Drug Discovery 140 4.7 Identification and Confirmation Process 141 4.7.1 Instrumentation 141 4.7.2 Data Analysis 143 4.8 Conclusion and Perspectives 148 References 149 5 MS Binding Assays 165 Georg Hofner and Klaus T.Wanner 5.1 Introduction 165 5.2 MS Binding Assays Strategy 167 5.2.1 Analogies and Differences Compared to Radioligand Binding Assays 167 5.2.2 Fundamental Assay Considerations 169 5.2.3 Fundamental Analytical Considerations 170 5.3 Application of MS Binding Assays 171 5.3.1 MS Binding Assays for the GABA Transporter GAT1 171 5.3.2 MS Binding Assays for the Serotonin Transporter 183 5.3.3 MS Binding Assays Based on the Quantitation of the Nonbound Marker 187 5.3.4 Other Examples Following the Concept of MS Binding Assays 189 5.4 Summary and Perspectives 191 Acknowledgments 192 References 192 6 Metabolic Profiling Approaches for the Identification of Bioactive Metabolites in Plants 199 Emily Pipan and Angela I. Calderon 6.1 Introduction to Plant Metabolic Profiling 199 6.2 Sample Collection and Processing 200 6.3 Hyphenated Techniques 203 6.3.1 Liquid Chromatography Mass Spectrometry 203 6.3.2 Gas Chromatography Mass Spectrometry 206 6.3.3 Capillary Electrophoresis Mass Spectrometry 207 6.4 Mass Spectrometry 207 6.4.1 Time of Flight 208 6.4.2 Quadrupole Mass Filter 208 6.4.3 Ion Traps (Orbitrap and Linear Quadrupole (LTQ)) 209 6.4.4 Fourier Transform Mass Spectrometry 210 6.4.5 Ion Mobility Mass Spectrometry 210 6.5 Mass Spectrometric Imaging 210 6.5.1 MALDI-MS 211 6.5.2 SIMS-MS 212 6.5.3 DESI-MS 212 6.5.4 LAESI-MS 213 6.5.5 LDI-MS and Others for Imaging 213 6.6 Data Analysis 214 6.6.1 Data Processing 214 6.6.2 Data Analysis Methods 214 6.6.3 Databases 215 6.7 Future Perspectives 216 References 216 7 Antivenomics: A Proteomics Tool for Studying the Immunoreactivity of Antivenoms 227 Juan J. Calvete, Jose Maria Gutierrez, Libia Sanz, Davinia Pla, and Bruno Lomonte 7.1 Introduction 227 7.2 Challenge of Fighting Human Envenoming by Snakebites 227 7.3 Toolbox for Studying the Immunological Profile of Antivenoms 228 7.4 First-Generation Antivenomics 229 7.5 Snake Venomics 230 7.6 Second-Generation Antivenomics 232 7.7 Concluding Remarks 236 Acknowledgments 236 References 236 Part III Direct Pre- and On-Column Coupled Techniques 241 8 Frontal and Zonal Affinity Chromatography Coupled to Mass Spectrometry 243 Nagendra S. Singh, Zhenjing Jiang, and Ruin Moaddel 8.1 Introduction 243 8.2 Frontal Affinity Chromatography 244 8.3 Staircase Method 247 8.4 Simultaneous Frontal Analysis of a Complex Mixture 249 8.5 Multiprotein Stationary Phase 252 8.6 Zonal Chromatography 253 8.7 Nonlinear Chromatography 260 Acknowledgments 265 References 265 9 Online Affinity Assessment and Immunoaffinity Sample Pretreatment in Capillary Electrophoresis Mass Spectrometry 271 Rob Haselberg and Govert W. Somsen 9.1 Introduction 271 9.2 Capillary Electrophoresis 272 9.3 Affinity Capillary Electrophoresis 276 9.3.1 Dynamic Equilibrium ACE (Fast Complexation Kinetics) 276 9.3.2 Pre-Equilibrium ACE (Slow Complexation Kinetics) 279 9.3.3 Kinetic ACE (Intermediate Complexation Kinetics) 280 9.4 Immunoaffinity Capillary Electrophoresis 281 9.5 Capillary Electrophoresis Mass Spectrometry 283 9.5.1 General Requirements for Effective CE MS Coupling 283 9.5.2 Specific Requirements for ACE MS and IA-CE-MS 284 9.6 Application of ACE MS 286 9.7 Applications of IA-CE MS 292 9.8 Conclusions 295 References 296 10 Label-Free Biosensor Affinity Analysis Coupled to Mass Spectrometry 299 David Bonnel, Dora Mehn, and Gerardo R. Marchesini 10.1 Introduction to MS-Coupled Biosensor Platforms 299 10.2 Strategies for Coupling Label-Free Analysis with Mass Spectrometry 301 10.2.1 On-Chip Approaches 301 10.2.2 Off-Chip Configurations 305 10.2.3 Chip Capture and Release Chromatography Electrospray-MS 306 10.3 New Sensor and MS Platforms, Opportunities for Integration 307 10.3.1 Imaging Nanoplasmonics 307 10.3.2 EvanescentWave SiliconWaveguides 308 10.3.3 New Trends in MS Matrix-Free Ion Sources 309 10.3.4 Tag-Mass 310 10.3.5 Integration 310 References 310 Part IV Direct Post Column Coupled Affinity Techniques 317 11 High-Resolution Screening: Post-Column Continuous-Flow Bioassays 319 David Falck, Wilfried M.A. Niessen, and Jeroen Kool 11.1 Introduction 319 11.1.1 Variants of On-line Post-Column Assays Using Mass Spectrometry 321 11.1.2 Targets and Analytes 328 11.2 The High-Resolution Screening Platform 330 11.2.1 Separation 330 11.2.2 Flow Splitting 334 11.2.3 Bioassay 336 11.2.4 MS Detection 340 11.3 Data Analysis 342 11.3.1 Differences between HRS and HTS 342 11.3.2 Validation 350 11.4 Conclusions and Perspectives 353 11.4.1 The Relation of On-line Post-Column Assays to Other Formats 353 11.4.2 Trends in High-Resolution Screening 354 11.4.3 Conclusions 357 References 358 12 Conclusions 365 Jeroen Kool Index 373.
  • (source: Nielsen Book Data)
This monograph reviews all relevant technologies based on mass spectrometry that are used to study or screen biological interactions in general. Arranged in three parts, the text begins by reviewing techniques nowadays almost considered classical, such as affinity chromatography and ultrafiltration, as well as the latest techniques. The second part focusses on all MS-based methods for the study of interactions of proteins with all classes of biomolecules. Besides pull down-based approaches, this section also emphasizes the use of ion mobility MS, capture-compound approaches, chemical proteomics and interactomics. The third and final part discusses other important technologies frequently employed in interaction studies, such as biosensors and microarrays. For pharmaceutical, analytical, protein, environmental and biochemists, as well as those working in pharmaceutical and analytical laboratories.
(source: Nielsen Book Data)
Biology Library (Falconer)
Book
1 online resource.
Anthropic Awareness: The Human Aspects of Scientific Thinking in NMR Spectroscopy and Mass Spectrometry blends psychology, philosophy, physics, mathematics, and chemistry, describing a human-centered philosophy of the essence of scientific thinking in the natural sciences and in everyday life. It addresses the reasons why we are prone to make errors in our conclusions and how to avoid such mistakes, also exploring a number of the "mental traps" that can lead to both individual mistakes and mass misconceptions. The book advocates that by understanding the nature of these mental traps we can adopt tactics to safely evade them. It includes Illustrative examples of common scientific misunderstandings and mental traps in both the theory and real-life application of NMR spectroscopy and mass spectrometry.
Book
1 online resource : illustrations (some color)
Volume 9: Historical Perspectives, Part A: The Development of Mass Spectrometry of The Encyclopedia of Mass Spectrometry describes and analyzes the development of many aspects of Mass Spectrometry. Beginning with the earliest types of Mass Analyzers, Historical Perspectives explores the development of many different forms of analytical processes and methods. The work follows various instruments and interfaces, to the current state of detectors and computerization. It traces the use of Mass Spectrometry across many different disciplines, including Organic Chemistry, Biochemistry, and Proteomics; Environmental Mass Spectrometry; Forensic Science; Imaging; Medical Monitoring and Diagnosis; Earth and Planetary Sciences; and Nuclear Science. Finally, the book covers the history of manufacturers and societies as well as the professionals who form the Mass Spectrometry community. Also available: Volume 9: Historical Perspectives, Part B: Notable People in Mass Spectrometry briefly reviews the lives and works of many of the major people who carried out this development. * Preserves the history and development of Mass Spectrometry for use across scientific fields * Written and edited by Mass Spectrometry experts* Coordinates with Volume 9: Historical Perspectives, Part B: Notable People in Mass Spectrometry, a collection of short biographies on many of the major people who carried out this development.
(source: Nielsen Book Data)
Book
1 online resource : illustrations (some color)
  • Cover; The Encyclopedia ofMass Spectrometry; Contents; Foreword; Preface to Volume 9, Part B; Introduction to Part B: Criteria for Inclusion in these Biographies of Notable People in Mass Spectrometry; References; Arthur John Ahearn; References; Claude Jean Allègre; References; Francis William Aston; References; Kenneth Tompkins Bainbridge; References; Michael Barber; References; Hans-Dieter Beckey; References; Alfred Benninghoven; References; John Herbert Beynon; References; Klaus Biemann; References; Walker Bleakney; References; John Hamilton Bowie; References; Harrison Scott Brown.
  • William J. HenzelReferences; David Michael Hercules; References; Zdenecaronk Herman; References; Richard Franz Karl Herzog; References; Franz Hillenkamp; References; Heinrich Hintenberger; References; John Alfred Hipple, Jr.; References; John Leonard Holmes; References; Richard Edward Honig; References; Evan Charles Horning and Marjorie Groothuis Horning; References; Robert Samuel Houk; References; Donald Frederick Hunt; References; Mark G. Inghram; References; Keith R. Jennings; References; Michael Karas; References; Paul Kebarle; References; Charles Kemball; References; Frederick W. Lampe.
Volume 9: Historical Perspectives, Part A: The Development of Mass Spectrometry of The Encyclopedia of Mass Spectrometry describes and analyzes the development of many aspects of Mass Spectrometry. Beginning with the earliest types of Mass Analyzers, Historical Perspectives explores the development of many different forms of analytical processes and methods. The work follows various instruments and interfaces, to the current state of detectors and computerization. It traces the use of Mass Spectrometry across many different disciplines, including Organic Chemistry, Biochemistry, and Proteomics; Environmental Mass Spectrometry; Forensic Science; Imaging; Medical Monitoring and Diagnosis; Earth and Planetary Sciences; and Nuclear Science. Finally, the book covers the history of manufacturers and societies as well as the professionals who form the Mass Spectrometry community. Also available: Volume 9: Historical Perspectives, Part B: Notable People in Mass Spectrometry briefly reviews the lives and works of many of the major people who carried out this development. * Preserves the history and development of Mass Spectrometry for use across scientific fields * Written and edited by Mass Spectrometry experts* Coordinates with Volume 9: Historical Perspectives, Part B: Notable People in Mass Spectrometry, a collection of short biographies on many of the major people who carried out this development.
(source: Nielsen Book Data)
Book
xviii, 606 p. : ill.
  • Liquid Chromatography-- UHPLC-- Sample Treatment-- Mass Spectrometry-- High Resolution Mass Spectrometry-- Sub-2mum Columns-- Core-Shell Columns-- Monolithic Columns, High-Temperature Liquid Chromatography-- HILIC-- Perfluorinated Stationary Phases-- Sample Treatment-- On-line SPE-- MIPs-- RAM-- Turbulent Flow Chromatography-- Ambient Source Ionization-- DESI-- DART-- DAPPI-- Quantification Aspects in MS-- Confirmation Aspects in MS-- Food Analysis-- Environmental Analysis-- Pesticides-- Polyphenolic Compounds-- Food Packaging Contaminants-- Perfluorinated Compounds-- Mycotoxins--.
  • (source: Nielsen Book Data)
There is a growing need for high-throughput separations in food and environmental research that are able to cope with the analysis of a large number of compounds in very complex matrices. Laboratories worldwide are now demanding fast and efficient analytical procedures with enough sensitivity, robustness, effectiveness and high resolution to be able to perform both qualitative and quantitative analysis while at the same time achieving cost-effective methodologies with reduced analysis times. Whereas the most common approach for solving many analytical problems has often been high-performance liquid chromatography (HPLC), the recent use of fast or ultra-fast chromatographic methods for environmental and food analysis has increased the overall sample throughput and laboratory efficiency without loss (and even with an improvement) in the resolution obtained by conventional HPLC systems.This book brings together researchers at the top of their field from across the world to discuss and analyze recent advances in fast liquid chromatography-mass spectrometry (LC-MS) methods for food and environmental analysis. It focuses on the development of the analytical method: sample preparation, chromatographic separation, mass spectrometry and, finally, confirmation and quantification aspects. These topics are addressed in three main parts. First, the most novel approaches to achieve fast and ultra-fast methods as well as the use of alternative and complementary stationary phases are described. In the second part, advances in fast LC-MS methods are addressed, focusing on novel treatment procedures coupled with LC-MS, new ionization sources, high-resolution mass spectrometry, and the problematic confirmation and quantification aspects in mass spectrometry. Finally, the third part is devoted to relevant LC-MS applications in food and environmental analysis and addresses the analysis of pesticides, mycotoxins, food packaging contaminants, perfluorinated compounds and polyphenolic compounds.This book brings together researchers working at the top of the field at universities and in industry from across the world to present the state-of-the-art in current research on food and environmental analysis. The scope of the book is intentionally broad and is aimed at worldwide analytical laboratories working in food and environmental applications as well as researchers in universities worldwide.
(source: Nielsen Book Data)
Book
1 online resource (Article No. 13121 ) : digital, PDF file.
An accurate and routinely available method for stoichiometric analysis of thin films is a desideratum of modern materials science where a material’s properties depend sensitively on elemental composition. We thoroughly investigated femtosecond laser ablation-inductively coupled plasma-mass spectrometry (fs-LA-ICP-MS) as an analytical technique for determination of the stoichiometry of thin films down to the nanometer scale. The use of femtosecond laser ablation allows for precise removal of material with high spatial and depth resolution that can be coupled to an ICP-MS to obtain elemental and isotopic information. We used molecular beam epitaxy-grown thin films of LaPd<sub>(x)</sub>Sb<sub>2</sub> and T´-La<sub>2</sub>CuO<sub>4</sub> to demonstrate the capacity of fs-LA-ICP-MS for stoichiometric analysis and the spatial and depth resolution of the technique. Here we demonstrate that the stoichiometric information of thin films with a thickness of ~10 nm or lower can be determined. Furthermore, our results indicate that fs-LA-ICP-MS provides precise information on the thin film-substrate interface and is able to detect the interdiffusion of cations.
Book
1 online resource.
Book
1 online resource (various pagings) : illustrations (some color).
  • Preface
  • Author biography
  • 1. Introduction
  • 1.1. Overview
  • 1.2. Basic principles
  • 2. Practical requirements
  • 2.1. Ion generation
  • 2.2. Primary and sputter ion beam sources
  • 2.3. Mass analysis
  • 2.4. Ion detection
  • 2.5. Ultra high vacuum
  • 3. Modes of analysis
  • 3.1. High-resolution mass spectra
  • 3.2. Depth profiling
  • 4. Ion beam-target interactions
  • 4.1. Ion beam induced atomic mixing
  • 4.2. Beam induced surface roughening and uneven etching
  • 4.3. Beam induced segregation
  • 4.4. Other beam induced effects
  • 4.5. Depth profiling with cluster ion beams
  • 5. Application to materials science
  • 5.1. Biomaterials and tissue studies
  • 5.2. Glass corrosion
  • 5.3. Ceramic oxides
  • 5.4. Semiconductor analysis
  • 5.5. Organic electronics
  • 6. Summary.
This book highlights the application of Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) for high-resolution surface analysis and characterization of materials. While providing a brief overview of the principles of SIMS, it also provides examples of how dual-beam ToF-SIMS is used to investigate a range of materials systems and properties. Over the years, SIMS instrumentation has dramatically changed since the earliest secondary ion mass spectrometers were first developed. Instruments were once dedicated to either the depth profiling of materials using high-ion-beam currents to analyse near surface to bulk regions of materials (dynamic SIMS), or time-of-flight instruments that produced complex mass spectra of the very outer-most surface of samples, using very low-beam currents (static SIMS). Now, with the development of dual-beam instruments these two very distinct fields now overlap.
Book
1 online resource
  • -1 Introduction -2 Fundamentals of Ion Chemistry -3 A theoretical Approach on ion-molecule complexation' -4 Experimental Methods and Instrumentation -5 Applications of Association Reactions in the Gas -6 Hybrid System with Ion Attachment Techniques -7 Cationization mass spectrometry for condensed-phase samples -8 Direct Analysis Mass Spectrometry -9 Summary and perspectives.
  • (source: Nielsen Book Data)
This book explores the mechanism of alkali-metal ion/molecule association reaction, surveys the instrumental basis to study its kinetic, and describes the instrumentation to the measurement of alkali-metal ion affinities. The applications of the ion complexation mechanism in the condensed phase in reaction to direct analysis MS are also covered. Other topics include mechanism and reaction rate, experimental and theoretical ion affinities, applications of ion attachment reactions (IAR) to mass spectrometry such as alkali ion CIMS, ion attachment MS and cationization mass spectrometry of ESI, FAB, FD, LD, MALDI and SIMS and topics of IAR-based direct analysis mass spectrometry.
(source: Nielsen Book Data)
Book
1 online resource : text file, PDF
  • Preface Isotope Ratio Mass Spectrometry: Devices, Methods, and Applications V.S. Sevastyanov General Characteristics of Mass Spectrometric Methods for the Determination of Isotopic Composition of Light Elements Improvement of Equipment for the Determination Isotope Ratios of Light Elements Mass Spectrometric Methods for Determining the Isotopic Composition of Light Elements Metrological Characteristics of Mass Spectrometry of Isotope Ratios The Effect of the Strength of the Analytical Signal on the Results of Measuring the Isotopic Composition of Light Elements Study of the Completeness of Combustion of Difficult to Oxidise Compounds Developing a New Method of Sample Preparation Based on Solid Electrolytes for Isotope Mass Spectrometric Analysis The Electrochemical Decomposition of Water using a Solid Electrolyte Based on Zirconium Dioxide to Determine the Isotopic Composition of Hydrogen The Distribution of the Isotopes of Light Elements in Various Objects Finding the Source of Drugs and Explosives The Effects of Isotope Fractionation and Accompanying Organic Synthesis Isotope Effects in Carbonaceous Chondrites Determination of the Isotopic Composition of Hydrogen and Oxygen of Water and Isotope Effects during Evaporation The Distribution of Carbon Isotopes in Complex Organic Compounds of Biological Origin (Oil and Hydrocarbon Gases) Determination of the Isotopic Composition of Carbon in the Collagen of Bones of Ancient Tombs Conclusions References Universal Method for Preparation of Liquid, Solid, and Gaseous Samples for Determining the Isotopic Composition of Carbon T.A. Velivetskaya, A. Ignat'ev, and S. Kiyashko Introduction The Experimental Part Equipment and Materials Combustion of Solid and Low-Volatility Liquid Samples to Determine the Isotopic Composition of Carbon Combustion of Volatile Liquids to Determine Carbon Isotopic Composition Combustion of Methane for the Determination of the Isotopic Composition of Carbon Results and Discussion Conclusions Literature Using Isotope Ratio Mass Spectrometry for Assessing the Metabolic Potential of Soil Microbiota A.M. Zyakun and O. Dilly Introduction Methods of Analysis of Microbial Activity in Soil Microbiological Method Isotope Ratio Mass Spectrometry in the Study of Substrate-Induced Respiration (SIR) Kinetics of CO2 Production during Substrate-Induced Respiration Characteristics of Carbon Isotopic Composition of Microbial Products Amount of Metabolic Carbon Dioxide and Characterization of Its Origin in the Soil Using the 13C/12C Ratios to Characterize the Activity of the Microbiota in Arable Soils Analyzed Soil Samples Mineralization of SOM and Exogenous Glucose Priming Effect (PE) of Glucose Estimate of the Duration of the Effect of the Exogenous Substrate on the Microbiota Conclusion References Study of the Isotopic Composition of Normal Alkanes of Continental Plants N.A. Pedentchouk Introduction The Experimental Part General Provisions Methodological Features of Analysis Results and Discussion Conclusion Literature Using Isotope Ratio Mass Spectroscopy for Analysis of Tobacco A.B. Uryupin Literature Using Isotope Mass Ratio Spectrometry of Carbon in Doping Control T. Sobolevski, I.S. Prasolov, and G.M. Rodchenkov Introduction The Metabolism of Steroid Hormones The Experimental Part Equipment Reagents and Materials Sample Preparation Results and Discussion Literature Isolation Methods in Isotope Geochemistry of Noble Gases A.I. Buikin Introduction Stepwise Annealing Release of Gases by Heating with a Laser Beam The Stratified Oxidation Method Stepwise Fragmentation Conclusion Literature Using Laser Spectroscopy for Measuring the Ratios of Stable Isotopes V.S. Sevastyanov Introduction Absorption Spectroscopy Infrared Spectroscopy with Fourier Transform Non-Dispersive Absorption Spectroscopy Laser Cavity Ring-Down Spectroscopy Laser On-Axis Integrated Cavity Output Spectroscopy New Methods and Results Conclusions Literature Index.
  • (source: Nielsen Book Data)
Isotope Ratio Mass Spectrometry of Light Gas-Forming Elements explores different methods of isotope analysis, including spark, secondary ion, laser, glow discharge, and isotope ratio mass spectrometry. It explains how to evaluate the isotopic composition of light elements (H, C, N, O) in solid, liquid, and gaseous samples of organic and inorganic substances, as well as: Presents a universal, economical, simple, and rapid technique for sample preparation of organic substances to measure the isotopic composition of carbon Describes how to determine microbial mineralization of organic matter in soil and the effect of exogenous substrates on environmental sustainability Examines use of the isotopic composition of n-alkanes from continental vegetation to study the paleoclimate and plant physiology Proposes a systematic approach to identifying tobacco areas of origin and tobacco products based on data from the isotopic composition of light elements Discusses ways to detect doping drugs and suggests results assessment criteria based on determining reference intervals for endogenous markers Reviews methods of release of gases from inclusions of rocks and minerals for further implementation of isotope mass spectrometric analysis Considers use of optical isotope analyzers for determining the isotopic composition of carbon in CO2 and of hydrogen and oxygen in water Providing a complete picture of the latest advancements in the field, Isotope Ratio Mass Spectrometry of Light Gas-Forming Elements aids readers from a variety of disciplines in identifying the fundamental processes in biological, ecological, and geological systems and in revealing the subtle features of many physicochemical processes and chemical transformations.
(source: Nielsen Book Data)
Book
1 online resource (viii, 71 pages) : illustrations.
  • General and most usual proteomics methods based on mass spectrometry analysis: Protein identification and protein quantification.- Direct analysis of tissues and body fluids.- Mass spectrometry in archaeology.- Determination and quantification of post-translational modifications.- Protein-Protein interaction determined by mass spectrometry.- Protein structure analysis.- Determination of protein function by mass spectrometry.- Computer-assisted data analysis and data mining for new applications.
  • (source: Nielsen Book Data)
The understanding of the events taking place in a cell, a biological fluid or in any biological system is the main goal of biology research. Many fields of research use different technology to assess those events. Mass spectrometry is one of those techniques and this undergoes constant evolution and adaptation to always enhance the accuracy of the information provided. Proteomics provides a large panel of data on protein identity and protein expression that were made possible by mass spectrometry. For several years now mass spectrometry has become central to performing proteomic research, however this powerful tool is under constant evolution to be more sensitive and more resolute. More importantly mass spectrometry became a field of research focusing on new applications. Indeed, the complexity in biological systems relies on the changes of expression of transcription of proteins but also on the post-translational modification of proteins, the structure of proteins and the interaction between proteins, amongst others. As of now, several investigations tried to improve the quantification of proteins by mass spectrometry, the determination of post-translational modifications, the protein-protein and protein-nucleic acids interaction or the proteins structures. This book is structured as follows: after a brief introduction of the usual and most popular applications for mass spectrometry in proteomics, the most recent research and developments in mass spectrometry-based methodologies will be explored.
(source: Nielsen Book Data)

Looking for different results?

Modify your search: Search all fields

Search elsewhere: Search WorldCat Search library website