Introduction.- Computational analysis of PAR-CLIP data.- Transcriptome-wide analysis of regulatory interactions of the RNA-binding protein HuR.- Binding site occupancy with competition interactions in equilibrium.- Circular RNAs are a large class of animal RNAs with regulatory potency.- Discussion.- Methods.
(source: Nielsen Book Data)
The work described in this book is an excellent example of interdisciplinary research in systems biology. It shows how concepts and approaches from the field of physics can be efficiently used to answer biological questions and reports on a novel methodology involving creative computer-based analyses of high-throughput biological data. Many of the findings described in the book, which are the result of collaborations between the author (a theoretical scientist) and experimental biologists and between different laboratories, have been published in high-quality peer-reviewed journals such as Molecular Cell and Nature. However, while those publications address different aspects of post-transcriptional gene regulation, this book provides readers with a complete, coherent and logical view of the research project as a whole. The introduction presents post-transcriptional gene regulation from a distinct angle, highlighting aspects of information theory and evolution and laying the groundwork for the questions addressed in the subsequent chapters, which concern the regulation of the transcriptome as the primary functional carrier of active genetic information. (source: Nielsen Book Data) 9783319070810 20160613
From the contents: Introduction: Hierarchical Bottom-Up Methodology for Integrating
Synthesis of Ethynylhelicene Oligomers
Homo-Double Helix Formation of Ethynylhelicene Oligomers Possessing Various Side Chains
Hetero-Double Helix Formation of Pseudoenantiomeric Ethynylhelicene Oligomers
Higher Assembly Formation of Pseudoenantiomeric Ethynylhelicene Oligomers Conclusions
In biological systems, molecules hierarchically form ordered assemblies and macroscopic substances, as exemplified by the assembly of proteins. The development of such systems using oligomeric macromolecules obtained by organic synthesis can provide insights into biological phenomena and will lead to the creation of new materials. In this regard, synthetic double helix molecules are attractive subjects of study because they have an important structural motif that appears widely in nature and can exhibit dynamic structural change. This thesis describes an unprecedented bottom-up approach using synthetic ethynylhelicene oligomers, which form dynamic double helices in organic media. Oligomer synthesis, homo- and hetero-double helix formation, and higher assembly formation due to intercomplex interactions are documented. The hierarchical assembly formation is a cogent reminder of the assembly of biological proteins that create living creatures. Also notable here is the demonstration of a methodology of providing diverse hetero-double helices and their higher assemblies by changing the combination of oligomers, which can be another advantage of this synthetic system.
Christopher Schirwitz's thesis focuses on improving the quality of in situ synthesized high-complexity peptide micro arrays. Micro arrays containing proteins or small protein fragments in the form of peptides have become of great interest in proteomic research. With the help of these microarrays a large number of potential target molecules can be screened for interaction with a probe in a short timeframe. However, protein and peptide micro arrays are still lagging behind oligonucleotide arrays in terms of density, quality and manufacturing costs. A new approach developed at the German Cancer Research Center (DKFZ) has improved the synthesis of high-density peptide arrays. The current technology is capable of producing arrays with up to 40,000 different peptides per square cm by means of micro particle-based solid phase peptide synthesis. However, in situ synthesis approaches bear a conceptual disadvantage: The quality of the peptides is dependent on the efficiency of the synthesis so that peptide fragments are present in the resulting array among the desired full-length peptides. In peptide-protein interaction studies such peptide fragments. The central achievement of this thesis is the development of a new method allowing for the fast one-step purification of entire arrays without loss of resolution or spatial information. Christopher Schirwitz's work has resulted in a number of publications in high ranking journals.
Bellingham, Wash. (1000 20th St. Bellingham WA 98225-6705 USA) : SPIE, 1999.
Book — 1 online resource (xix, 208 p.) : ill.
Modulation Threshold and Noise-- Model for the Spatial Contrast Sensitivity of the Eye-- Extension of the Contrast Sensitivity Model to Extra-Foveal Vision-- Extension of the Contrast Sensitivity Model to the Temporal Domain-- Effect of Nonwhite Spatial Noise on Contrast Sensitivity-- Contrast Discrimination Model-- Image Quality Measure-- Effect of Various Parameters on Image Quality.
(source: Nielsen Book Data)
This book examines the contrast sensitivity of the human visual system - concerning the eye's ability to distinguish objects from each other or from the background - and its effects on the imageforming process. The text provides equations for determining various aspects of contrast sensitivity, in addition to models that easily can be used for practical applications. (source: Nielsen Book Data) 9780819434968 20160605