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Book
xix, 1293 pages ; 24 cm
In this Completely Revised and Extended Edition with a significantly enhanced printed content, all Chapters have been updated considering relevant literature and recent developments until 2015 together with application oriented examples with a focus on Industrial Biocatalysis. Newly treated topics comprise among others systems metabolic engineering approaches, metagenome screening, new tools for pathway engineering, and de-novo computational design as actual research areas in biocatalysis. Information about different aspects of RNA technologies, and completely new Chapters on 'Fluorescent Proteins' and 'Biocatalysis and Nanotechnology' are also included.
(source: Nielsen Book Data)9781783269082 20171121
Science Library (Li and Ma)
Book
xvii, 786 pages ; 26 cm.
  • Preface Part I: Macroscopic Fluid Mechanics Chapter 1: Introduction to Fluid Mechanics Chapter 2: Mass, Energy, and Momentum Balances Chapter 3: Fluid Friction in Pipes Chapter 4: Flow in Chemical Engineering Equipment Part II: Microscopic Fluid Mechanics Chapter 5: Differential Equations of Fluid Mechanics Chapter 6: Solution of Viscous-Flow Problems Chapter 7: Laplace's Equation, Irrotational and Porous-Media Flows Chapter 8: Boundary-Layer and Other Nearly Unidirectional Flows Chapter 9: Turbulent Flow Chapter 10: Bubble Motion, Two-Phase Flow, and Fluidization Chapter 11: Non-Newtonian Fluids Chapter 12: Microfluidics and Electrokinetic Flow Effects Chapter 13: An Introduction to Computational Fluid Dynamics and ANSYS Fluent Chapter 14: COMSOL Multiphysics for Solving Fluid Mechanics Problems Appendix A: Useful Mathematical Relationships Appendix B: Answers to the True/False Assertions Appendix C: Some Vector and Tensor Operations General Index COMSOL Multiphysics Index The Authors.
  • (source: Nielsen Book Data)9780134712826 20171017
The Chemical Engineer's Practical Guide to Fluid Mechanics: Now Includes COMSOL Multiphysics 5 Since most chemical processing applications are conducted either partially or totally in the fluid phase, chemical engineers need mastery of fluid mechanics. Such knowledge is especially valuable in the biochemical, chemical, energy, fermentation, materials, mining, petroleum, pharmaceuticals, polymer, and waste-processing industries. Fluid Mechanics for Chemical Engineers: with Microfluidics, CFD, and COMSOL Multiphysics 5, Third Edition, systematically introduces fluid mechanics from the perspective of the chemical engineer who must understand actual physical behavior and solve real-world problems. Building on the book that earned Choice Magazine's Outstanding Academic Title award, this edition also thoroughly introduces the popular COMSOL Multiphysics 5 software. This third edition contains extensive coverage of both microfluidics and computational fluid dynamics, systematically demonstrating CFD through detailed examples using COMSOL Multiphysics 5 and ANSYS Fluent. The chapter on turbulence now presents valuable CFD techniques to investigate practical situations such as turbulent mixing and recirculating flows. Part I offers a clear, succinct, easy-to-follow introduction to macroscopic fluid mechanics, including physical properties; hydrostatics; basic rate laws; and fundamental principles of flow through equipment. Part II turns to microscopic fluid mechanics. Differential equations of fluid mechanicsViscous-flow problems, some including polymer processingLaplace's equation, irrotational, and porous-media flowsNearly unidirectional flows, from boundary layers to lubrication, calendering, and thin-film applicationsTurbulent flows, showing how the k/ε method extends conventional mixing-length theoryBubble motion, two-phase flow, and fluidizationNon-Newtonian fluids, including inelastic and viscoelastic fluidsMicrofluidics and electrokinetic flow effects including electroosmosis, electrophoresis, streaming potentials, and electroosmotic switchingComputational fluid mechanics with ANSYS Fluent and COMSOL Multiphysics Nearly 100 completely worked practical examples include ten new COMSOL 5 examples: boundary layer flow, non-Newtonian flow, jet flow, lathe flow, lubrication, momentum diffusion, turbulent flow, and others. More than 300 end-of-chapter problems of varying complexity are presented, including several from University of Cambridge exams. The author covers all material needed for the fluid mechanics portion of the professional engineer's exam.
(source: Nielsen Book Data)9780134712826 20171017
Science Library (Li and Ma)
Book
xix, 695 pages : illustrations ; 24 cm.
  • Preface xiii Part 1 Strategies of Affinity Materials 1 Recent Molecularly Imprinted Polymer-based Methods for Sample Preparation 3 Antonio Martin-Esteban 1.1 Introduction 3 1.2 Molecularly Imprinted Solid-phase Extraction 6 1.3 Molecularly Imprinted Solid-phase Microextraction 14 1.4 Molecularly Imprinted Stir Bar Sorptive Extraction 17 1.5 Other Formats 18 1.6 Conclusions 20 References 21 2 A Genuine Combination of Solvent-free Sample Preparation Technique and Molecularly Imprinted Nanomaterials 29 Santanu Patra, Ekta Roy, Rashmi Madhuri and Prashant K. Sharma 2.1 Introduction 30 2.2 Molecularly Imprinted Polymer Modified Fiber for Solid-phase Microextraction 40 2.3 In-tube Solid-phase Microextraction Technique 55 2.4 Monolithic Fiber 58 2.5 Micro-solid-phase Extraction 70 2.6 Stir-bar Sorptive Extraction 73 2.7 Conclusion and Future Scope 76 Acknowledgments 76 Abbreviations 77 References 78 3 Fluorescent Molecularly Imprinted Polymers 89 Kornelia Gawlitza, Wei Wan, Sabine Wagner and Knut Rurack 3.1 Introduction 89 3.2 Classes of Emitters to Endow MIPs with Fluorescence 91 3.3 Fluorescent Molecularly Imprinted Silica 108 3.4 Post-imprinting of MIPs 111 3.5 fMIPs as Labels 113 3.6 Formats for fMIPs 115 3.7 Conclusion 119 References 120 4 Molecularly Imprinted Polymer-based Micro- and Nanotraps for Solid-phase Extraction 129 R dvan Say, Rustem Kecili and Arzu Ersoz 4.1 Introduction 130 4.2 MIPs as SPE Materials 130 4.3 Conclusions 149 References 153 5 Imprinted Carbonaceous Nanomaterials: A Tiny Looking Big Thing in the Field of Selective and Secific Analysis 165 Ekta Roy, Santanu Patra, Rashmi Madhuri and Prashant K. Sharma 5.1 Introduction 166 5.2 Graphene-modified Imprinted Polymer 179 5.3 Carbon Nanotubes-modified Imprinted Polymer 190 5.4 Combination of graphene, CNTs, and MIPs 197 5.5 Graphene Quantum Dots and/or Carbon Dots 198 5.6 Fullerene 201 5.7 Activated carbon 202 5.8 Conclusions 203 Acknowledgments 204 List of abbreviations 204 References 205 6 Molecularly Imprinted Materials for Fiber-optic Sensor Platforms 217 Yavuz Orhan Yaman, Necdet Ba aran, Kubra Karayagiz, Zafer Vatansever, Cengiz Yegin, Onder Haluk Tekba and Mufrettin Murat Sari 6.1 Introduction 218 6.2 Material Aspect: Morphology and Physical Forms of MIPs in FO Sensors 223 6.3 Molecularly Imprinting Technology for Fiber-optic Sensors 231 6.4 State-of-the-art Fiber-optic Sensors Applications Using Molecularly Imprinted Materials 268 6.5 Conclusion 273 References 274 Part 2 Rational Design of MIP for Advanced Applications 7 Molecularly Imprinted Polymer-based Sensors for Biomedical and Environmental Applications 285 Anca Florea, Oana Hosu, Bianca Ciui and Cecilia Cristea 7.1 Introduction 285 7.2 Molecularly Imprinted Polymers for Analytes of Biomedical Interest 296 7.3 Molecularly Imprinted Polymers for Analytes of Environmental Interest 306 7.4 Conclusion 314 Acknowledgments 316 References 316 8 Molecularly Imprinted Polymers: The Affinity Adsorbents for Environmental Biotechnology 327 Bo Mattiasson and Gizem Erturk 8.1 Introduction 327 8.2 Molecularly Imprinted Polymers 329 8.3 Monomers 329 8.4 Cross-linking Agents 331 8.5 Mode of Polymerization 332 8.6 Cryogels 334 8.7 Process Technology 336 8.8 Applications 338 References 345 9 Molecular Imprinting Technology for Sensing and Separation in Food Safety 353 Baran Onal Ulusoy, Mehmet Odaba i and Ne e Hayat Aksoy 9.1 Food Safety 354 9.2 Food Analysis 355 9.3 Current Separation Methods Used for Food Safety Purposes 356 9.4 What Is MIP? 357 9.5 MIP Applications Used for Food Safety Purposes 359 References 377 10 Advanced Imprinted Materials for Virus Monitoring 389 Zeynep Altintas 10.1 Introduction 390 10.2 Virus Imprinting 393 10.3 Artificial MIP Receptors for Viruses 398 10.4 Virus Monitoring and Detection Using Biomimetic Sensors 399 10.5 Virus Imprinting for Separation Technologies 401 10.6 Conclusions 405 References 406 11 Design and Evaluation of Molecularly Imprinted Polymers as Drug Delivery Systems 413 Andre Luis Morais Ruela and Gislaine Ribeiro Pereira 11.1 Introduction 414 11.2 Synthesis and Characterization of MIPs Intended for Drug Release Using Non-covalent Approaches 418 11.3 Design and Evaluation of Drug Delivery Systems Based on MIPs 436 11.4 Conclusions 445 References 446 12 Molecularly Imprinted Materials for Controlled Release Systems 455 Yagmur Yegin, Gokhan Yilmaz, Omer Karakoc, Cengiz Yegin, Servet Cete, Mustafa Akbulut and Mufrettin Murat Sari 12.1 Introduction 456 12.2 Selectivity, Release Mechanism and Functionality of MIPs-based CR Systems 459 12.3 Molecularly Imprinted Polymers Production for Controlled Release 482 12.4 Controlled Release Applications Using Molecularly Imprinted Materials-based Controlled Release 491 12.5 Conclusion 506 References 507 13 Molecular Imprinting: The Creation of Biorecognition Imprints on the Biosensor Surfaces 523 Gizem Erturk and Bo Mattiasson 13.1 Introduction 523 13.2 Molecular Imprinting 524 13.3 Microcontact Imprinting 525 13.4 Capacitive Biosensors 529 13.5 Surface Plasmon Resonance Biosensors 541 13.6 Concluding Remarks 549 References 550 14 Molecular Imprinted Polymers for Sensing of Volatile Organic Compounds in Human Body Odor 561 Sunil Kr. Jha 14.1 Introduction 562 14.2 MIP-QCM Sensor Array Preparation 573 14.3 Chemical Vapor Sensing 576 14.4 Analysis Outcomes 603 14.5 Conclusion 624 Acknowledgments 624 References 624 15 Development of Molecularly Imprinted Polymer-based Microcantilever Sensor System 637 Meltem Okan and Memed Duman 15.1 Introduction to Mass Sensors 637 15.2 Principles of Mass Sensors 640 15.4 Molecularly Imprinted Polymer Technology 655 15.5 Molecularly Imprinted Polymer-based QCM Sensors 658 15.6 Ongoing Studies on Molecularly Imprinted Polymers-based Microcantilevers 661 Acknowledgments 669 References 669.
  • (source: Nielsen Book Data)9781119336297 20161213
Molecularly imprinted polymers (MIPs) are an important functional material because of their potential implications in diverse research fields. The materials have been developed for a range of uses including separation, environmental, biomedical and sensor applications. In this book, the chapters are clustered into two main sections: Strategies to be employed when using the affinity materials, and rational design of MIPs for advanced applications. In the first part, the book covers the recent advances in producing MIPs for sample design, preparation and characterizations. In the second part, the chapters demonstrate the importance and novelty of creation of recognition imprinted on the materials and surfaces for a range of microbial detection sensors in the biomedical, environmental and food safety fields as well as sensing human odor and virus monitoring systems. Part 1: Strategies of affinity materials * Molecularly imprinted polymers * MIP nanomaterials * Micro- and nanotraps for solid phase extraction * Carbonaceous affinity nanomaterials * Fluorescent MIPs * MIP-based fiber optic sensors Part 2: Rational design of MIP for advanced applications * MIP-based biomedical and environmental sensors * Affinity adsorbents for environmental biotechnology * MIP in food safety * MIP-based virus monitoring * MIP-based drug delivery and controlled release * Biorecognition imprints on the biosensor surfaces * MIP-based sensing of volatile organic compounds in human body odour * MIP-based microcantilever sensor system.
(source: Nielsen Book Data)9781119336297 20161213
Science Library (Li and Ma)
Book
xxvi, 835 pages ; 24 cm.
  • Fundamentals of NanotechnologyTorben Kodanek, Sara Sanchez Paradinas, Franziska Lubkemann, Dirk Dorfs, and Nadja C. BigallBiocatalysis: An IntroductionPeter GrunwaldEnvironmentally Benign Nanomaterial Synthesis Mediated by Culture BrothsLihong Liu, Fang Xie, Xiuxia Meng, Vishnu Parek, and Shaomin LiuRational Design of Enzyme-Polymer BiocatalystsOmkar V. Zore, Rajeswari M. Kasi, and Challa V. KumarBiological Strategies in Nanobiocatalyst AssemblyIan Dominic F. Tabanag, and Shen-Long TsaiGraphene-Based Nanobiocatalytic SystemMichaela Patilaa , George Orfanakisa , Angeliki C. Polydera, Ioannis V. Pavlidis, and Haralambos StamatisImmobilization of Biocatalysts onto Nanosupports: Advantages for Green TechnologiesAlan S. Campbell, Andrew J. Maloney, Chenbo Dong, and Cerasela Z. DinuEnzyme Immobilization on Membrane and Its Application in BioreactorsPeng-Cheng Chen, Xue-Yan Zhu, Jin Lia, and Xiao-Jun HuangPotential Applications of Nanobiocatalysis for Industrial Biodiesel ProductionAvinesh Byreddy and Munish PuriEnzymogel Nanoparticles Chemistry for Highly Efficient Phase Boundary BiocatalysisAnaniy Kohut, Scott W. Pryor, Andriy Voronov, and Sergiy MinkoRecent Advances in Nanostructured Enzyme Catalysis for Chemical Synthesis in Organic SolventsZheng Liu, Jun Ge, Diannan Lu, Guoqiang Jiang, and Jianzhong WuProbing Enzymatic Activity by Combining Luminescent Quantum Dots, Gold Nanoparticles and Energy TransferNaiqian Zhan, Goutam Palui, Wentao Wang, and Hedi MattoussiFRET Reporter Molecules for Identification of Enzyme FunctionsJing Mu, Hao Lun Cheong, and Bengang XingQuantum Dot Architectures on Electrodes for Photoelectrochemical Analyte DetectionMark Riedel, Daniel Schafer, and Fred LisdatInorganic Nanoparticles as Enzyme MimicsRuben Ragg, Karsten Korschelt, Karoline Herget, Filipe Natalio, Muhammad Nawaz Tahir, and Wolfgang TremelEnzyme Nanocapsules for Glucose Sensing and Insulin DeliveryWanyi Tai and Zhen GuNanostructured Materials for Enzymatic Biofuel CellsTakanori TamakiEnzymatic Biofuel Cells on Porous NanostructuresDan Wen and Alexander EychmullerNanoplasmonic BiosensorsBruno P. Crulhas, Caroline R. Basso, and Valber A. PedrosaEnzyme Biocomputing: Logic Gates and Logic Networks to Interface and Control MaterialsMarcos PitaFunctional Nano-Bio Conjugates for Targeted Cellular Uptake and Specific Nanoparticle-Protein InteractionsSanjay Mathur, Shaista Ilyas, Laura Wortmann, Jasleen Kaur, Isabel GessnerCell-Free Expression-Based Microarrays: Applications and Future ProspectsApurva Atak and Sanjeeva SrivastavaOverview of the Current Knowledge and Challenges Associated With Human Exposure to NanomaterialsAli Kermanizadeh, Kim Jantzen, Astrid Skovmand, Ana C. D. Gouveia, Nicklas R. Jacobsen, Vicki Stone, and Martin J. D. Clift.
  • (source: Nielsen Book Data)9789814613699 20180115
Nanobiocatalysis has rapidly developed into a subarea of enzyme biotechnology. It combines the advances in nanotechnology that have generated nanoscale materials of different sizes, shapes, and physicochemical properties, and the excellent characteristics of biocatalysts into an innovative technology. This book provides an overview of the various relations between nanotechnology and biocatalysis. It discusses the fabrication and application of nanomaterials for the immobilization of enzymes used in the sustainable production of goods and chemicals. Nanosupports have several advantages compared with bulk solid materials because of their high surface area, which results in a significantly reduced mass transfer limitation and comparatively high enzyme loading. These characteristics are also of great use for applications in the fields of enzymatic biosensors, biofuel cells, bioelectronics, and photoelectrochemical analyte detection, where conductive nanomaterials improve the rate of electron transfer. The book also presents an overview of nanotoxicology and covers nanostructured enzyme catalysis in organic solvents and its potential application for biodiesel production, probing of enzymatic activity, and identification of enzyme functions of inorganic nanoparticles as enzyme mimics.
(source: Nielsen Book Data)9789814613699 20180115
Science Library (Li and Ma)
Book
x, 282 pages ; 24 cm.
  • Introduction
  • Development of Microsystems Multi Physics Investigation Methods
  • MEMS applications for obesity prevention
  • MOEMS-assisted radial pulse measurement system development
  • Microsystems for the Effective Technological Processes.
This book presents the most important aspects of analysis of dynamical processes taking place on the human body surface. It provides an overview of the major devices that act as a prevention measure to boost a person's motivation for physical activity. A short overview of the most popular MEMS sensors for biomedical applications is given. The development and validation of a multi-level computational model that combines mathematical models of an accelerometer and reduced human body surface tissue is presented. Subsequently, results of finite element analysis are used together with experimental data to evaluate rheological properties of not only human skin but skeletal joints as well. Methodology of development of MOEMS displacement-pressure sensor and adaptation for real-time biological information monitoring, namely "ex vivo" and "in vitro" blood pulse type analysis, is described. Fundamental and conciliatory investigations, achieved knowledge and scientific experience about biologically adaptive multifunctional nanocomposite materials, their properties and synthesis compatibility, periodical microstructures, which may be used in various optical components for modern, productive sensors' formation technologies and their application in medicine, pharmacy industries and environmental monitoring, are presented and analyzed. This book also is aimed at research and development of vibrational energy harvester, which would convert ambient kinetic energy into electrical energy by means of the impact-type piezoelectric transducer. The book proposes possible prototypes of devices for non-invasive real-time artery pulse measurements and micro energy harvesting.
(source: Nielsen Book Data)9783319548487 20170724
Science Library (Li and Ma)
Book
xi, 314 pages : illustrations (some color) ; 25 cm.
  • 35 Years of Marine Natural Product Research in Sweden: Cool Molecules and Models from Cold Waters.- Major Antimicrobial Representatives from Marine Sponges and/or Their Associated Bacteria.- Discovery and Development of Novel Drugs.- Computer Aided Drug Discovery from Marine Compounds: Identification of the Three-dimensional Structural Features Responsible for Antimalarial Activity.- BlueGenics: Bioactive Natural Products of Medicinal Relevance and Approaches to Their Diversification.- New Target Sites for Treatment of Osteoporosis .- Biocalcite and Carbonic Acid Activators.- Electrospinning of Bioactive Wound Healing Nets.- Entotheonella Bacteria as Source of Sponge-derived Natural Products: Opportunities for Biotechnological Production.
  • (source: Nielsen Book Data)9783319512822 20170502
This book describes the discovery of molecules from unexploited extreme marine environments, and presents new approaches in marine genomics. It combines the current state of knowledge in marine genomics and advanced natural products' chemistry to pursue the sustainable production of novel secondary metabolites (lead compounds), as well as pharmacologically active peptides/proteins, with antimicrobial, neuroprotective, anti-osteoporotic, anti-protozoan/anti-plasmodial, anti-ageing and immune-modulating effects. Further, it employs molecular-biology-based approaches and advanced chemical techniques to obtain and to select candidate compounds for pre-clinical and clinical studies.
(source: Nielsen Book Data)9783319512822 20170502
Science Library (Li and Ma)
Book
xxiv, 348 pages : color illustrations ; 23 cm
Science Library (Li and Ma)
Book
xvi, 399 pages : illustrations ; 25 cm
  • Process Analysis - The Importance of Mass and Energy Balances-- Introduction to Chemical Reaction Engineering-- Concepts of Fluid Flow-- An Introduction to Heat Transfer-- An Introduction to Mass-Transfer Operations-- Scale-Up in Chemical Engineering-- An Introduction to Particle Systems-- An Introduction to Process Control-- Economic Appraisal of Large Projects-- Process and Personal Safety-- Engineering Statistics. Process Integration, and Experimental Design-- Subject Index.
  • (source: Nielsen Book Data)9781782623588 20161114
Based on a former popular course of the same title, Concepts of Chemical Engineering for Chemists outlines the basic aspects of chemical engineering for chemistry professionals. It clarifies the terminology used and explains the systems methodology approach to process design and operation for chemists with limited chemical engineering knowledge. The book provides practical insights into all areas of chemical engineering with well explained worked examples and case studies. The new edition contains a revised chapter on Process Analysis and two new chapters "Process and Personal Safety" and "Systems Integration and Experimental Design", the latter drawing together material covered in the previous chapters so that readers can design and test their own pilot process systems. This book is a guide for chemists (and other scientists) who either work alongside chemical engineers or who are undertaking chemical engineering-type projects and who wish to communicate with their colleagues and understand chemical engineering principles.
(source: Nielsen Book Data)9781782623588 20161114
Science Library (Li and Ma)
Book
ix, 308 pages : illustrations (some color) ; 26 cm
  • Preface IX 1 Introduction to Directed Evolution 1 1.1 General Definition and Purpose of Directed Evolution of Enzymes 1 1.2 Brief Account of the History of Directed Evolution 4 1.3 Applications of Directed Evolution of Enzymes 16 References 17 2 Selection versus Screening in Directed Evolution 27 2.1 Selection Systems 27 2.2 Screening Systems 44 2.3 Conclusions and Perspectives 52 References 53 3 Gene Mutagenesis Methods 59 3.1 Introductory Remarks 59 3.2 Error-Prone Polymerase Chain Reaction (epPCR) and Other Whole-Gene Mutagenesis Techniques 60 3.3 Saturation Mutagenesis: Away from Blind Directed Evolution 70 3.4 Recombinant Gene Mutagenesis Methods 85 3.5 Circular Permutation and Other Domain Swapping Techniques 91 3.6 Solid-Phase Combinatorial Gene Synthesis for Library Creation 92 3.7 Computational Tools 96 References 101 4 Strategies for Applying Gene Mutagenesis Methods 115 4.1 General Guidelines 115 4.2 Rare Cases of Comparative Studies 118 4.3 Choosing the Best Strategy when Applying Saturation Mutagenesis 130 4.3.1 General Guidelines 130 4.3.2 Choosing Optimal Pathways in Iterative Saturation Mutagenesis (ISM) 135 4.3.3 Systematization of Saturation Mutagenesis 142 4.3.4 Single Code Saturation Mutagenesis (SCSM): Use of a Single Amino Acid as Building Block 149 4.3.5 Triple Code Saturation Mutagenesis (TCSM): A Viable Compromise when Choosing the Optimal Reduced Amino Acid Alphabet 151 4.4 Techno-Economical Analyses of Saturation Mutagenesis Strategies 154 4.5 Combinatorial Solid-Phase Gene Synthesis: An Alternative for the Future? 159 References 160 5 Selected Examples of Directed Evolution of Enzymes with Emphasis on Stereo- and Regioselectivity, Substrate Scope, and/or Activity 167 5.1 Explanatory Remarks 167 5.2 Collection of Selected Examples from the Literature 2010 up to 2016 189 References 189 6 Directed Evolution of Enzyme Robustness 205 6.1 Introduction 205 6.2 Application of epPCR and DNA Shuffling 207 6.3 B-FIT Approach 211 6.4 Iterative Saturation Mutagenesis (ISM) at Protein Protein Interfacial Sites for Multimeric Enzymes 215 6.5 Ancestral and Consensus Approaches and their Structure-Guided Extensions 216 6.6 Computationally Guided Methods 219 6.6.1 SCHEMA Approach 219 6.6.2 FRESCO Approach 221 6.6.3 FireProt Approach 223 6.6.4 Constrained Network Analysis (CNA) Approach 224 6.6.5 Alternative Approaches 226 References 227 7 Directed Evolution of Promiscuity: Artificial Enzymes as Catalysts in Organic Chemistry 237 7.1 Introductory Background Information 237 7.2 Tuning the Catalytic Profile of Promiscuous Enzymes by Directed Evolution 245 7.3 Conclusions and Perspectives 259 References 260 8 Learning from Directed Evolution 267 8.1 Background Information 267 8.2 Case Studies Featuring Mechanistic, Structural, and/or Computational Analyses of the Source of Evolved Stereo- and/or Regioselectivity 269 8.2.1 Epoxide Hydrolase 269 8.2.2 Ene-Reductase of the Old Yellow Enzyme (OYE) 273 8.2.3 Esterase 279 8.2.4 Cytochrome P450 Monooxygenase 282 8.3 Additive versus Non-additive Mutational Effects in Fitness Landscapes 287 References 296 Index 303.
  • (source: Nielsen Book Data)9783527316601 20170130
Authored by one of the world's leading organic chemists, this authoritative reference provides an overview of basic strategies in directed evolution and introduces common gene mutagenesis, screening and selection methods. Throughout the text, emphasis is placed on methodology development to maximize efficiency, reliability and speed of the experiments and to provide guidelines for efficient protein engineering. Professor Reetz highlights the application of directed evolution experiments to address limitations in the field of enzyme selectivity, substrate scope, activity and robustness. He critically reviews recent developments and case studies, takes a look at future applications in the field of organic synthesis, and concludes with lessons learned from previous experiments.
(source: Nielsen Book Data)9783527316601 20170130
Science Library (Li and Ma)
Book
xiv, 594 pages : illustrations ; 26 cm.
Science Library (Li and Ma)
Book
xxxiv, 607 pages : illustrations ; 25 cm
  • List of Figures Xi List of Tables Xvii Abbreviations Xix Glossary Xxiii 1 Process Safety and Safe Automation 1 1.1 Objective 7 1.2 Scope 9 1.3 Limitations 9 1.4 Target Audience 11 1.5 Incidents That Define Safe Automation 13 1.6 Overview of the Contents 18 1.7 Key Differences 21 2 The Role of Automation in Process Safety 23 2.1 Process Operations 23 2.2 Plant Automation 33 2.3 A Framework for Process Safety 42 2.4 Risk-Based Design 54 2.5 Risk Management of Existing Facility 78 3 Automation Specification 83 3.1 Process Automation Lifecycle 83 3.2 Functional Specification 91 3.3 Designing For Operating Objectives 92 3.4 Inherently Safer Practices 104 3.5 Designing for Core Attributes 107 3.6 Control and Safety System Integration 133 4 Design And Implementation Of Process Control Systems 153 4.1 Input and Output Field Signal Types 161 4.2 Basic Application Program Functions 162 4.3 Process Control Objectives 165 4.4 Process Controller Technology Selection 172 4.5 Detailed Application Program Design 194 5 Design and Implementation of Safety Controls, Alarms, and Interlocks (SCAI) 211 5.1 SCAI Classification 215 5.2 Design Considerations 220 5.3 SCAI Technology Selection 244 6 Administrative Controls and Monitoring 265 6.1 Introduction 265 6.2 Automation Organization Management 266 6.3 Process Safety Information 269 6.4 Operating Procedures 273 6.5 Maintenance Planning 291 6.6 Human and Systematic Failure Management 303 6.7 Management of Change 316 6.8 Auditing, Monitoring and Metrics 321 Appendix A. Control System Considerations 329 Appendix B. Power, Grounding, and Shielding 371 Appendix C. Communications 391 C.1 Communication Classifications 391 C.2 Common Communication Network Topologies 395 C.3 Communication between Devices 397 C.4 Wireless Communication 400 C.5 Common Communication Configurations 403 C.6 Common Data Communication Issues 407 C.7 Process Control and Safety System Communications 412 C.8 SCAI Communications 419 Appendix D. Alarm Management 423 D.1 Alarms 423 D.2 Standards and Resources 423 D.3 Alarm Management 423 D.4 Managing the Safety Aspects Of Alarms 436 D.5 Alarm System Performance Benchmarking 437 D.6 Alarm Management Software 438 Appendix E. Field Device Considerations 441 E.1 General Signal Safety 441 E.2 Field Device Selection 458 E.3 Flow Measurement 465 E.4 Pressure Measurement 475 E.5 Level Measurement 476 E.6 Temperature Measurement 487 E.7 On-Stream Process Analysis 489 E.8 Automated Valves 493 E.9 Electric Motors 504 E.10 Steam Turbine Variable Speed Drives 505 Appendix F. Sis Equipment Selection 511 F.1 Selection Basis 511 F.2 Additional Considerations 518 Appendix G. Human Machine Interface Design 529 G.1 General 529 G.2 Operator Interface Standards and Resources 531 G.3 Instrument Panels 533 G.4 Configurable Operator Workstations 534 G.5 Process Alarms 538 G.6 Sis Impact on HMI 545 G.7 Control-Center Environment 545 G.8 Video 546 G.9 Operator Interfaces Of Future 546 G.10 HMI Considerations Checklist 547 Appendix H. Application Programming 551 H.1 Software Types 551 H.2 Application Program Development 552 H.3 Application Programming Languages 554 H.4 Application Program Developmental Models 556 H.5 Process Control Application Program 557 H.6 SCAI Application Program 563 Appendix I. Instrument Reliability Program 565 I.1 Introduction 565 I.2 Tracking Failure 566 I.3 Data Taxonomy 568 I.4 Data Collection Efforts 569 I.5 Failure Investigation 571 I.6 Calculation of Failure Rate 572 I.7 Verification 576 Appendix J. Acceptance Testing Guidelines 581 J.1 Acceptance Testing 581 J.2 Standards 581 J.3 Factory Acceptance Test 582 J.4 Site Acceptance Test (SAT) 589 Index 597.
  • (source: Nielsen Book Data)9781118949498 20170213
This book provides designers and operators of chemical process facilities with a general philosophy and approach to safe automation, including independent layers of safety. An expanded edition, this book includes a revision of original concepts as well as chapters that address new topics such as use of wireless automation and Safety Instrumented Systems. This book also provides an extensive bibliography to related publications and topic-specific information.
(source: Nielsen Book Data)9781118949498 20170213
Science Library (Li and Ma)
Book
xxiii, 696 pages : illustrations ; 26 cm
  • Architecture of Mathematical Models Introduction Classification of Mathematical Models in Chemical and Biological Engineering Models Resulting in Algebraic Equations: Lumped-Parameter, Steady-State Models Models Resulting in Ordinary Differential Equations: Initial Value Problems Models Resulting in Ordinary Differential Equations: Boundary Value Problems Models Resulting in Partial Differential Equations Model Equations in Non-Dimensional Form Concluding Comments Exercise Problems References Ordinary Differential Equations and Applications Introduction Review of Solution of Ordinary Differential Equations The Laplace Transform Technique Matrix Method of Solution of Simultaneous ODEs Concluding Comments Exercise Problems References Special Functions and Solutions of Ordinary Differential Equations with Variable Coefficients Introduction The Gamma Function The Beta Function The Error Function The Gamma Distribution Series Solution of Linear Second-Order ODEs with Variable Coefficients Series Solution of Linear Second-Order ODEs Leading to Special Functions Legendre Differential Equation and the Legendre Functions Hypergeometric Functions Concluding Comments Exercise Problems References Partial Differential Equations Introduction Common Second Order PDEs in Science and Engineering Boundary Value Problems Types of Boundary Conditions Techniques of Analytical Solution of a Second Order PDE Examples: Use of the Technique of Separation of Variables Solution of Non-Homogeneous PDEs Similarity Solution Moving Boundary Problems Principle of Superposition Green's Function Concluding Comments Exercise Problems References Integral Transforms Introduction Definition of an Integral Transform Fourier Transform Laplace Transform Application to Engineering Problems Concluding Comments Exercise Problems References Approximate Methods of Solution of Model Equations Introduction Order Symbols Asymptotic Expansion Perturbation Methods Concluding Comments Exercise Problems References Answers to Selected Exercise Problems Appendix A: Topics in Matrices Appendix B: Fourier Series Expansion and Fourier Integral Theorem Appendix C: Review of Complex Variables Appendix D: Selected Formulas and Identities-- Dirac Delta Function and Heaviside Function Appendix E: Brief Table of Inverse Laplace Transforms Appendix F: Some Detailed Derivations View a List of Solved Examples.
  • (source: Nielsen Book Data)9781482210385 20170306
Mathematical Methods in Chemical and Biological Engineering describes basic to moderately advanced mathematical techniques useful for shaping the model-based analysis of chemical and biological engineering systems. Covering an ideal balance of basic mathematical principles and applications to physico-chemical problems, this book presents examples drawn from recent scientific and technical literature on chemical engineering, biological and biomedical engineering, food processing, and a variety of diffusional problems to demonstrate the real-world value of the mathematical methods. Emphasis is placed on the background and physical understanding of the problems to prepare students for future challenging and innovative applications.
(source: Nielsen Book Data)9781482210385 20170306
Science Library (Li and Ma)
Book
xix, 338 pages, 12 unnumbered pages of plates : illustrations (some color) ; 28 cm
  • Preface, vii Acknowledgements, ix Introduction to biotechnology lab, xi About the companion website, xix Part I: METHODS IN BIOTECHNOLOGY (MB) LABORATORY EXERCISES 1 1 MB experiment 1: Lab measurements, 3 2 MB experiment 2: Use of the spectrophotometer and Beer s law, 9 3 MB experiment 3: Making solutions and buffer efficacy, 15 4 MB experiment 4: Acid base titration, 19 5 MB experiment 5: Protein denaturation and precipitation, 23 6 MB experiment 6: Bacterial transformation, 27 7 MB experiment 7: GFP purification, 33 8 MB experiment 8: SDS-PAGE analysis, 37 9 MB experiment 9: DNA isolation, 41 10 MB experiment 10: PCR-based Alu-human DNA typing, 45 11 MB experiment 11: Restriction enzyme digestion, 51 12 MB experiment 12: Agarose gel electrophoresis, 53 13 MB experiment 13: Ouchterlony and ELISA immunoassays, 57 14 MB experiment 14: Testing plant substances for antimicrobial activity, 63 15 MB experiment 15: Peroxidase enzyme activity assay, 67 Part II: ADVANCED METHODS IN BIOTECHNOLOGY (AMB) 1 LABORATORY EXERCISES 71 16 AMB 1 experiment 16: Aseptic technique and culture handling, 73 17 AMB 1 experiment 17: Yeast culture media preparation, 77 18 AMB 1 experiment 18: Growth curve, 79 19 AMB 1 experiment 19: Mini plasmid prep, 83 20 AMB 1 experiment 20: Restriction digestion, purification, concentration, and quantification of DNA, 87 21 AMB 1 experiment 21: Polymerase chain reaction (PCR), 91 22 AMB 1 experiment 22: TA, blunt end, SLIC, and CPEC cloning of PCR product, 97 23 AMB 1 experiment 23: One-step multifragment assembly cloning, 103 24 AMB 1 experiment 24: Restriction enzyme digestion and fast agarose gel electrophoresis, 111 25 AMB 1 experiment 25: Southern blot transfer, 117 26 AMB 1 experiment 26: Probe labeling and purification, 121 27 AMB 1 experiment 27: Dot blot assay, 125 28 AMB 1 experiment 28: Pre-hybridization, hybridization, and detection, 127 29 AMB 1 experiment 29: Total yeast RNA isolation and RT-PCR, 131 30 AMB 1 experiment 30: Yeast-based in vivo recombination cloning, 139 31 AMB 1 experiment 31: Plasmid DNA isolation from yeast, 143 32 AMB 1 experiment 32: E. coli transformation with yeast plasmid DNA, 145 33 AMB 1 experiment 33: X-gal filter lift assay, 147 34 AMB 1 experiment 34: Protein quantitation assay, 149 35 AMB 1 experiment 35: Quantitative -Galactosidase assay in yeast, 155 36 AMB 1 experiment 36: Gel filtration chromatography (GFC), 161 37 AMB 1 experiment 37: Ion exchange chromatography (IEC), 165 Part III: ADVANCED METHODS IN BIOTECHNOLOGY (AMB) 2 LABORATORY EXERCISES 169 38 AMB 2 experiment 38: E. coli culture media preparation, 171 39 AMB 2 experiment 39: Site-directed mutagenesis, 175 40 AMB 2 experiment 40: Protein expression in E. coli, 179 41 AMB 2 experiment 41: Protein purification by affinity column chromatography, 187 42 AMB 2 experiment 42: SDS-PAGE analysis of affinity column fractions, 193 43 AMB 2 experiment 43: Western blot analysis of affinity column fractions, 199 44 AMB 2 experiment 44: Yeast media preparation and phenotypic analysis of yeast strains, 203 45 AMB 2 experiment 45: Yeast transformation for yeast two-hybrid (Y2H) assay and genome editing by CRISPR-Cas system, 209 46 AMB 2 experiment 46: Yeast mating-mediated Y2H assay and genomic PCR, 215 47 AMB 2 experiment 47: Yeast colony PCR screening and cycle DNA sequencing, 221 48 AMB 2 experiment 48: DNA sequencing electrophoresis, 227 49 AMB 2 experiment 49: RNA interference, 237 50 AMB 2 experiment 50: Protein preparation for 2D gel electrophoresis, 241 51 AMB 2 experiment 51: Two-dimensional gel electrophoresis, 245 Part IV: APPENDICES 253 1 Methods in Biotechnology Appendix 1, 255 2 MB Appendix 2, 261 3 MB Appendix 3, 265 4 MB Appendix 4, 267 5 AMB 1 Appendix 1, 271 6 AMB 1 Appendix 2, 281 7 AMB 1 Appendix 3, 287 8 AMB 1 Appendix 4, 291 9 AMB 1 Appendix 5, 297 10 AMB 2 Appendix 1, 299 11 AMB 2 Appendix 2, 307 12 AMB 2 Appendix 3, 313 13 AMB 2 Appendix 4, 317 14 AMB 2 Appendix 5, 319 15 AMB 2 Appendix 6, 321 16 AMB 2 Appendix 7, 325 Glossary, 327 Abbreviations, 335 Index, 337.
  • (source: Nielsen Book Data)9781119156789 20161114
As rapid advances in biotechnology occur, there is a need for a pedagogical tool to aid current students and laboratory professionals in biotechnological methods; Methods in Biotechnology is an invaluable resource for those students and professionals. Methods in Biotechnology engages the reader by implementing an active learning approach, provided advanced study questions, as well as pre- and post-lab questions for each lab protocol. These self-directed study sections encourage the reader to not just perform experiments but to engage with the material on a higher level, utilizing critical thinking and troubleshooting skills. This text is broken into three sections based on level Methods in Biotechnology, Advanced Methods in Biotechnology I, and Advanced Methods in Biotechnology II. Each section contains 14-22 lab exercises, with instructor notes in appendices as well as an answer guide as a part of the book companion site. This text will be an excellent resource for both students and laboratory professionals in the biotechnology field.
(source: Nielsen Book Data)9781119156789 20161114
Science Library (Li and Ma)
Book
xv, 182 pages : illustrations (some color) ; 25 cm.
  • Foreword.- Introduction.- About protocells.- Why modelling protocells.- Collective self-replication.- Self-replication in a vesicle.- Self-replication in a reproducing protocell.- Generic properties of dynamical models of protocells.- Introduction. -Generic properties of biological systems: data.- Generic properties of biological systems: concepts.- What shall we model.- Dynamical models of protocells and synchronization.- Simplified surface-reaction models of protocells.- Synchronization in surface reaction models.- Several linearly interacting replicators.- Several interacting replicators with nonlinear interactions.- Internal reaction models.- Models of self-replication.- Introduction.- Autocatalytic sets.- The properties of some replication models.- Products and substrates.- Reflexive autocatalytic food-generated (raf) sets.- A stochastic model of growing and dividing protocells.- Semipermeable protocells.- The role of active membranes.- The effects of passive membranes.- Coupled dynamics of rafs and protocells.- Maintaining novelties.-A comment on evolvable populations of protocells.- Conclusions, open questions and perspectives.- Introduction.- The hypothesis of spontaneous fission and synchronization.- The formation of self-sustaining autocatalytic cycles.- The role of membranes.- A virtual laboratory.
  • (source: Nielsen Book Data)9789402411584 20171218
The monograph discusses models of synthetic protocells, which are cell-like structures obtained from non-living matter endowed with some rudimentary kind of metabolism and genetics, but much simpler than biological cells. They should grow and proliferate, generating offsprings that resemble in some way the parent protocells with some variation, so that selection may take place. Sustainable protocell populations have not yet been obtained experimentally and mathematical models are therefore extremely important to address key questions concerning their synthesis and behavior. Different protocell "architectures" have been proposed and high-level abstract models like those that are presented in this book are particularly relevant to gain a better understanding of the different properites. These models are able to treat all the major dynamical phenomena in a unified framework, so they can be seen as "virtual laboratories" for protocell research. Particular attention is paid to the problem of synchronization of the fission rate of the whole protocell and the duplication rate of its "protogenetic" material, which is shown to be an emergent property that spontaneously develops in successive generations.The book is of interest for a broad range of scientists working in soft matter physics, chemistry and biology, interested in the role protocells may play on the development of new technologies with medical, environmental and industrial applications as well as scientists interested in the origin of life.
(source: Nielsen Book Data)9789402411584 20171218
Science Library (Li and Ma)
Book
ix, 306 pages ; 25 cm.
This book describes the latest molecular insights needed to understand the chemical and biological (CB) agents and their associated biotechnologies. Its primary focus is to present and discuss molecular technologies such as mass spectrometry, chemical and biological sensors, chromatographic and electrophoretic separation, and comparisons of spectroscopic, immunological and molecular analyses of chemicals used for the detection of chemical and biological agents and to prevent terrorism. This NATO-ASI book also contributes to the critical assessment of existing knowledge on new and important detection technologies. It helps to identify directions for future research and to promote closer working relationships between scientists from different professional fields.
(source: Nielsen Book Data)9789402411126 20171030
Science Library (Li and Ma)
Book
x, 125 pages ; 23 cm
Science Library (Li and Ma)
Book
xxii, 488 pages : illustrations ; 26 cm.
  • 1. Mathematical modeling and structured programming-- 2. Linear algebraic equations-- 3. Nonlinear algebraic equations-- 4. Initial value problems-- 5. Dynamical systems-- 6. Boundary value problems-- 7. Partial differential equations-- 8. Interpolation and integration-- Appendix A. MATLAB tutorial-- Appendix B. Determinant of a matrix.
  • (source: Nielsen Book Data)9781107135116 20170313
Designed primarily for undergraduates, but also graduates and practitioners, this textbook integrates numerical methods and programming with applications from chemical engineering. Combining mathematical rigor with an informal writing style, it thoroughly introduces the theory underlying numerical methods, its translation into MATLAB programs, and its use for solving realistic problems. Specific topics covered include accuracy, convergence and numerical stability, as well as stiffness and ill-conditioning. MATLAB codes are developed from scratch, and their implementation is explained in detail, all while assuming limited programming knowledge. All scripts employed are downloadable, and built-in MATLAB functions are discussed and contextualised. Numerous examples and homework problems - from simple questions to extended case studies - accompany the text, allowing students to develop a deep appreciation for the range of real chemical engineering problems that can be solved using numerical methods. This is the ideal resource for a single-semester course on numerical methods, as well as other chemical engineering courses taught over multiple semesters.
(source: Nielsen Book Data)9781107135116 20170313
Science Library (Li and Ma)
Book
ix, 502 pages : illustrations ; 25 cm
Science Library (Li and Ma)
Book
xl, 1109 pages : illustrations ; 24 cm
The Definitive, Up-to-Date, Student-Friendly Guide to Separation Process Engineering-With More Mass Transfer Coverage and a New Chapter on Crystallization Separation Process Engineering, Fourth Edition, is the most comprehensive, accessible guide available on modern separation processes and the fundamentals of mass transfer. In this completely updated edition, Phillip C. Wankat teaches each key concept through detailed, realistic examples using real data-including up-to-date simulation practice and spreadsheet-based exercises. Wankat thoroughly covers each separation process, including flash, column, and batch distillation; exact calculations and shortcut methods for multicomponent distillation; staged and packed column design; absorption; stripping; and more. This edition provides expanded coverage of mass transfer and diffusion, so faculty can cover separations and mass transfer in one course. Detailed discussions of liquid-liquid extraction, adsorption, chromatography, and ion exchange prepare students for advanced work. Wankat presents coverage of membrane separations, including gas permeation, reverse osmosis, ultrafiltration, pervaporation, and applications. An updated chapter on economics and energy conservation in distillation adds coverage of equipment costs. This edition contains more than 300 new, up-to-date homework problems, extensively tested in undergraduate courses at Purdue University and the University of Canterbury (New Zealand). Coverage includes * New chapter on crystallization from solution, including equilibrium, chemical purity, crystal size distribution, and pharmaceutical applications* Thirteen up-to-date Aspen Plus process simulation labs, adaptable to any simulator* Eight detailed Aspen Chromatography labs* Extensive new coverage of ternary stage-by-stage distillation calculations* Fraction collection and multicomponent calculations for simple batch distillation* New mass transfer analysis sections on numerical solution for variable diffusivity* Mass transfer to expanding or contracting objects, including ternary mass transfer* Expanded coverage of pervaporation* Updated Excel spreadsheets offering more practice with distillation, diffusion, mass transfer, and membrane separation problems.
(source: Nielsen Book Data)9780133443653 20161108
Science Library (Li and Ma)
Book
viii, 280 pages ; 27 cm.
Science Library (Li and Ma)