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• Cover; Title Page; Copyright Page; Brief Contents; Contents; Preface; Acknowledgments; About the Author;
• Chapter 1 The Microbial World: An Introduction; Good Morning Microbes!; 1.1 Microbiology and Society: A Connected World; 1.2 Why Microbes Matter: Protecting the Planet; The Ocean Microbiome; The Soil Microbiome; The Microbiome of the Atmosphere; The Microbiome in Earth's Crust; 1.3 Why Microbes Matter: Helping Maintain Human Health; 1.4 Why Microbes Matter: Causing Infections and Disease; 1.5 Why Microbes Matter: Benefiting Society; With Most Meals; In the Environment

• A CLOSER LOOK 1.1: Microbes to the Rescue!In the Pharmaceutical/Biotechnology Industries; 1.6 Microbiology Today: Challenges Remain; Air Travel; Urbanization and Poverty; Drug-Resistant Pathogens; Climate Change; A Final Thought; Chapter Discussion Questions;
• Chapter 2 Microbes in Perspective: The Pioneers, Classifiers, and Observers; What Shall We Call This One?; 2.1 The Roots of Microbiology: The Pioneers; Robert Hooke (1635-1703); Antony van Leeuwenhoek (1632-1723); Louis Pasteur (1822-1895); A CLOSER LOOK 2.1: Experimentation and Scientific Inquiry; Robert Koch (1843-1910)

• The Golden Age of Microbiology (1857-1917)2.2 Naming Microbes: What's in a Name?; Binomial Nomenclature; 2.3 The Microbial World: The Observers; Microbial Measurements and Cell Size; A CLOSER LOOK 2.2: "What's in a Name?"; The Light Microscope; The Electron Microscope; 2.4 Microbial Groups: Eukaryotes, Prokaryotes, and Viruses; Eukaryotic Microorganisms; Prokaryotic Organisms; The Viruses; 2.5 Taxonomy: Cataloging Life; Cataloging and Organizing Life; The Tree of Life; The Tree of Life and Society; A Final Thought; Chapter Discussion Questions

• Chapter 3 Molecules of the Cell: The Building Blocks of LifeThe Spark; 3.1 Chemistry Basics: Atoms, Bonds, and Molecules; Atoms and Bonding; Water; A CLOSER LOOK 3.1: Water and Life; The Molecules of Microbes; 3.2 Carbohydrates: Simple Sugars and Polysaccharides; Simple Sugars; Oligosaccharides and Polysaccharides; A CLOSER LOOK 3.2: How Sweet It Is; 3.3 Lipids: Fats, Phospholipids, and Sterols; Fats and Oils; Phospholipids; Sterols; 3.4 Proteins: Amino Acids and Polypeptides; Amino Acids; Polypeptides and Protein Shape; 3.5 Nucleic Acids: DNA and RNA; A Final Thought

• Chapter Discussion Questions
• Chapter 4 Exploring the Prokaryotic World: The Bacteria and Archaea Domains; Hail Caesar!; A CLOSER LOOK 4.1: Bacteria in Eight Easy Lessons1; 4.1 Cell Structure: Shapes and Arrangements; Prokaryotic Cell Shape and Arrangement; Staining Techniques; 4.2 Cell Structure: Anatomy of a Cell; Surface Structures; Surface Projections; A CLOSER LOOK 4.2: Diarrhea Doozies; Cytoplasmic Structures; 4.3 Prokaryotic Reproduction and Survival: Binary Fission and Endospores; Prokaryotic Reproduction; Measuring Prokaryotic Growth; Endospores

• Series Preface Preface Editors Contributors
• Chapter 1 When does symbiosis begin? Bacterial cues necessary for metamorphosis in the marine polychaete Hydroides elegans - Marnie Freckelton and Brian T. Nedved
• Chapter 2 The language of symbiosis: insights from protist biology - Morgan J. Colp and John M. Archibald
• Chapter 3 Trichoplax and its bacteria: How many are there? Are they speaking? - Michael G. Hadfield and Margaret J. McFall-Ngai
• Chapter 4 Decoding cellular dialogues between sponges, bacteria and phages - Lara Schmittmann, Martin T. Jahn, Lucia Pita and Ute Hentschel
• Chapter 5 Symbiotic interactions in the holobiont Hydra - Jay Bathia and Thomas C.G. Bosch
• Chapter 6 Hydra and Curvibacter - an intimate crosstalk at the epithelial interface - Timo Minten-Lange and Sebastian Fraune
• Chapter 7 The coral holobiont highlights the dependence of cnidarian animal hosts on their
• associated microbes - Claudia Pogoreutz, Christian R Voolstra, Nils Radecker, Virginia Weis, Anny Cardenas and Jean-Baptiste Raina
• Chapter 8 Extra-intestinal regulation of the gut microbiome: The case of C. elegans TGF /SMA signaling - Rebecca Choi, Dan Kim, Stacy Li, Meril Massot, Vivek Narayan, Samuel Slowinski, Hinrich Schulenburg and Michael Shapira
• Chapter 9 Multiple roles of bacterially produced natural products in the bryozoan Bugula neritina - Nicole B. Lopanik
• Chapter 10 The molecular dialogue through ontogeny between a squid host and its luminous symbiont - Margaret J. McFall-Ngai
• Chapter 11 Evolving integrated multipartite symbioses between plant-sap feeding insects (Hemiptera) and their endosymbionts - Gordon Bennett
• Chapter 12 Symbiosis for insect cuticle formation - Hisashi Anbutsu and Takema Fukatsu
• Chapter 13 Microbial determinants of folivory in insects - Aileen Berasategui and Hassan Salem
• Chapter 14 Right on cue: microbiota promote plasticity of zebrafish digestive tract - Michelle S. Massaquoi and Karen J. Guillemin
• Chapter 15 Uncovering the history of intestinal host-microbiome interactions through vertebrate comparative genomics - Colin R. Lickwar and John F. Rawls
• Chapter 16 Molecular interactions of microbes and the plant phyllosphere: The phyllosphere microbiome is shaped by the interplay of secreted microbial molecules and the plant immune system - Janine Haueisen, Cecile Lorrain and Eva H. Stukenbrock
• Chapter 17 Summing up: Cellular dialogues between hosts and microbial symbionts: generalities emerging - Michael C. G. Hadfield and Thomas G. Bosch.
• (source: Nielsen Book Data)

This book examines how the growing knowledge of the huge range of protist-, animal-, and plant-bacterial interactions, whether in shared ecosystems or intimate symbioses, is fundamentally altering our understanding of biology. The establishment and maintenance of these interactions and their contributions to the health and survival of all partners relies on continuous cell-to-cell communication between them. This dialogue may be concerned with all aspects of the biology of both partners. The book includes chapters devoted to exploring, explaining, and exposing these dialogues across a broad spectrum of plant and animal eukaryotes to a broad field of biologists. Key Features Explores the nature of the interactions between eukaryotic hosts and their microbial symbionts Examines the links between prostist, animal, and plant evolution and microbial communities Reviews specific taxa and the microbial diversity associated with these taxa Illustrates the role microbes play in the physiology and etiology of several model species Includes chapters by an international team of leading scholars.
(source: Nielsen Book Data)

• Part I: Vector design For vaccination
• 1. A Novel Cre Recombinase Mediated In Vivo Minicircle (CRIM) DNA Vaccine Platform for Veterinary Application
• Yanlong Jiang, Guilian Yang, and Chunfeng Wang
• 2. Vaccination with Messenger RNA - a Promising Alternative to DNA Vaccination
• Istvan Tombacz, Drew Weissman, and Norbert Pardi
• 3. Impact of a Plasmid DNA-Based Alphavirus Vaccine on Immunization Efficiency
• Kenneth Lundstrom
• Part II: DNA Vaccine Adjuvants and Immunostimulatory Response
• 4. CpG Oligonucleotides as Vaccine Adjuvants
• Neslihan Kayraklioglu, Begum Horuluoglu, and Dennis M. Klinman
• 5. Molecular Adjuvants for DNA Vaccines: Application, Design, Preparation and Formulation
• Ailar Sabbaghi and Amir Ghaemi
• 6. Assessing Antigen-specific Cellular Immune Responses Upon HIV/SIV Plasmid DNA Vaccination in the Non-human Primate Model
• Xintao Hu, Barbara K. Felber, and Antonio Valentin
• Part III
• Biotechnological Processes to Obtain DNA Vaccines
• 7. Enhanced Biosynthesis of Plasmid DNA from Escherichia coli Applying Experimental Design
• Luis A. Passarinha
• 8. Primary Purification of Plasmid DNA Using Differential Isopropanol Precipitation
• Alexandra Wagner, A. Rita Silva-Santos, Sara Sousa Rosa, Sophie Gierak, Ana M. Azevedo, and Duarte Miguel F. Prazeres
• 9. Scale-Up of Plasmid DNA Downstream Process Based on Chromatographic Monoliths
• Urh Cernigoj and Ales Strancar
• 10. Purification of Plasmid DNA by Multimodal Chromatography
• A. Rita Silva-Santos, Sara Sousa Rosa, Duarte Miguel F. Prazeres, and Ana M. Azevedo
• 11. Minicircle DNA Vaccine Purification and E7 Antigen Expression Assessment
• Ana M. Almeida, Dalinda Eusebio, Joao A. Queiroz, Fani Sousa, and Angela Sousa
• Part IV:
• DNA Vaccine Delivery
• 12. Tumor-Specific CD8+ T Cell Responses Induced by DNA Vaccination
• Pablo Caceres-Morgado and Alvaro Lladser
• 13. Therapeutic DNA Vaccine Against HPV16-Associated Cancer
• Meihua Yu and Janin Chandra
• 14. Bulk and Microfluidic Synthesis of Stealth and Cationic-Liposomes for Gene Delivery Applications
• Lucimara Gaziola de la Torre, Amanda da Costa e Silva de Noronha Pessoa, Bruna Gregatti de Carvalho, Thiago Bezerra Taketa, Ismail Es, and Gabriel Perli
• 15. Conception of Plasmid DNA and Polyethylenimine Delivery Systems with Potential Application in DNA Vaccines Field
• Diana Costa, Angela Sousa, Ruben Faria, Ana Raquel Neves, and Joao A. Queiroz
• 16. Main Features of DNA Based Vectors for Use in Lactic Acid Bacteria and Update Protocols
• Nina D. Coelho-Rocha, Fernanda A. L. Barroso, Laisa M. Tavares, Ester S. S. dos Santos, Vasco Azevedo, Mariana M. Drumond, and Pamela Mancha-Agresti
• Part V:
• DNA Vaccine Transfer to Clinical Trials
• 17. Ethics of DNA Vaccines Transfer for Clinical Research
• Ana Cristina Ramalhinho and Miguel Castelo-Branco
• 18. Preparation of an Academic Clinical TrialPreparation of an academic clinical trial
• Ana Cristina Ramalhinho and Miguel Castelo-Branco.
• (source: Nielsen Book Data)

This volume details practical procedures on the latest DNA vaccine technology. Chapters guide readers through methods and protocols on DNA vaccine design, the adjuvant influence, production and purification methodologies, delivery systems, and approaches of the influence of DNA vaccines in the immunological response performance and in the cancer immunotherapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Vaccines: Methods and Protocols aims to ensure successful results in the further study of this vital field.
(source: Nielsen Book Data)

• Contents Foreword ix
• Preface xi
• Acknowledgment xiii
• Author xv
• Introduction xvii
• 1 Endophytes in the Tropics 1
• 1.1 Diversity and Ubiquity 1
• 1.2 Endophytes and Their Tropical Host Plants 4
• 1.3 Factors Driving Endophyte Communities in the Tropics 14
• 1.4 Spread of Tropical Endophytes and Their Endophyte-Host
• Plant Association 15
• 1.5 Endophyte Research in the Tropics 18
• 2 Applications of Endophytes from the Tropics 21
• 2.1 Introduction 21
• 2.2 Tropical Endophytes for Agriculture 22
• 2.3 Tropical Endophytes for Biomedicine and Biosynthesis
• of Valuable Compounds 28
• 2.3.1 Antimicrobial Compounds 28
• 2.3.2 Anticancer Compounds 30
• 2.3.3 Functional Polysaccharides 36
• 2.3.4 Other Valuable Compounds/Metabolites 36
• 2.3.5 Biotransformation of Valuable Compounds 39
• 2.3.6 Biosynthesis of Nanoparticles by Tropical
• Endophytes 42
• 2.4 Tropical Endophytes for Industrial Use 43
• 2.4.1 Enzymes from Tropical Endophytes 43
• 2.4.2 Pigments (or Colorants) from Tropical Endophytes 45
• 2.5 Tropical Endophytes for Bioenergy and Biocatalysis 48
• 2.6 Tropical Endophytes for Bioremediation 49
• 2.6.1 Metal Bioremediation 49
• 2.6.2 Hydrocarbon Bioremediation 54
• 2.6.3 Other Xenobiotic Pollutants 55
• 3 Valuable Endophytic Species from the Tropics 57
• 3.1 Endophytic Actinobacteria 57
• 3.2 Diaporthe and Phomopsis Species 58
• 3.3 Lasiodiplodia Species 59
• 3.4 Muscodor Species 60
• 3.5 Trichoderma Species 60
• 3.6 Xylaria Species 61
• 4 Commercialization of Endophytes from the Tropics 63
• 4.1 Introduction 63
• 5 Challenges in Endophytic Research in the Tropics 65
• 5.1 Challenges and Limitations 65
• 5.2 Remedial Strategies to Challenges 67
• 6 Conclusions 71
• References 73
• Index 105.
• (source: Nielsen Book Data)

Endophytes of the Tropics: Diversity, Ubiquity and Applications (R) is a presentation of consolidated information on endophytes from the tropical region. It provides a glimpse of the various species of endophytes in the tropics, their host plants, their applications in the various field, and some of the challenges in endophytic research. In short, this book presents an overview of and the future of endophyte research in the tropics. The book offers comprehensive coverage of the most essential topics, including: Diversity of endophytes in the tropics Beneficial application of tropical endophytes or their derivatives Highlights on some valuable endophytic species from the tropics Some commercialized products based on tropical endophytes Challenges in researching endophytes Remedial strategies to advance research on tropical endophytes This books stands apart from others as it highlights on endophytes of the tropical regions, associating the uniqueness of tropical endophytes with the rich plant diversity and ethnobotanical history. There is therefore greater possibility that novel metabolites can be isolated from the endophytes, which can be explored and harnessed for the benefit of mankind.
(source: Nielsen Book Data)

• 1. Introduction
• 2. The Basics of Molecular Pathology
• 3. The Biochemical Basis of in situ Hybridization and Immunohistochemistry
• 4. The Basics of in situ Hybridization
• 5. The Basics of Immunohistochemistry
• 6. Quality Control for Immunohistochemistry and in situ Hybridization: How to Know if the Color Change Is Signal or Background
• 7. Recent Improvements in Immunohistochemistry and in situ Hybridization
• 8. The Basics of Histologic Interpretations of Tissues
• 9. The Recommended Protocol for in situ Hybridization
• 10. The Recommended Protocol for Immunohistochemistry
• 11. Coexpression Analyses
• 12. PCR in situ Hybridization and RT in situ PCR.
• (source: Nielsen Book Data)

Major advancements in the field of in situ molecular pathology have occurred since publication of the first edition. In Situ Molecular Pathology and Co-expression Analyses, Second Edition, continues to teach both the molecular basis for the improvements and the actual protocols. This is the unique feature that separates it from the pack of other "cook-book" type approaches. The fields of in situ hybridization and immunohistochemistry have expanded rapidly where computer-based analyses systems have greatly expanded the power of these methods. Further, knowledge of the marked improvements in the reagents themselves since the first edition can make the difference of excellent versus misleading data. The automated platforms require that researchers and diagnostic biomedical investigators have a good understanding of the basics of in situ based tests, protocols, and biochemistry for troubleshooting in order to maximize the use of these platforms. This second edition focuses attention on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses. Practicing molecular pathologists, diagnostic pathologists, laboratory directors, and toxicologists, as well as clinicians and researchers in training, will benefit from this clear presentation of protocols and theoretical framework. Data derived from in situ hybridization and immunohistochemistry.
(source: Nielsen Book Data)

• Life in extreme environments: insights in biological capability-- Introduction Guido di Prisco, Ad Huiskes, Josef Elster and Howell Edwards-- Part I. Extreme Environments: Responses and Adaptation to Change:
• 1. Physiological traits of the Greenland shark somniosus microcephalus obtained during the TUNU-expeditions to northeast Greenland Guido di Prisco, Nicoletta Ademollo, Stefania Ancora, Jorgen S. Christiansen, Daniela Coppola, Simonetta Corsolini, Sara Ferrando, Laura Ghigliotti, Daniela Giordano, Arve Lynghammar, Julius Nielsen, Eva Pisano, Roberta Russo, John F. Steffensen and Cinzia Verde--
• 2. Metazoan adaptation to deep-sea hydrothermal vents Stephane Hourdez and Didier Jollivet--
• 3. Extremophiles populating a high level natural radiation area (HLNRA) in northern Iran Fatemeh Heidari, Hossein Riahi and Zeinab Shariatmadari-- Part II. Biodiversity, Bioenergetic Processes, and Biotic and Abiotic Interactions:
• 4. Metazoan life in anoxic marine sediments Roberto Danovaro, Cinzia Corinaldesi, Antonio Dell'Anno, Cristina Gambi, Antonio Pusceddu and Michael Tangherlini--
• 5. How to survive winter? Adaptation and acclimation strategies of eukaryotic algae in polar terrestrial ecosystems Martina Pichrtova, Eva Hejdukova, Linda Nedbalova and Josef Elster--
• 6. Viral infections of vertebrates including humans in the polar areas Jiri Cerny, Jana Elsterova, Daniel Ruzek and Libor Grubhoffer-- Part III. Life in Extreme Environments and the Responses to Change: The Example of Polar Environments:
• 7. Life in the extreme environments of our planet under pressure: climate-induced threats and exploitation opportunities Melody S. Clark, Cinzia Verde, Silvia Fineschi, Francesco Loreto, Lloyd S. Peck and Guido di Prisco--
• 8. The ecophysiology of responding to change in polar marine benthos Lloyd S. Peck--
• 9. The Southern Ocean: an extreme environment or just home of unique ecosystems? Julian Gutt and Gerhard Dieckmann--
• 10. Microorganisms in cryoturbated organic matter of arctic permafrost soils Jiri Barta--
• 11. Chemical ecology in the Southern Ocean Carlos Angulo-Preckler, Paula de Castro-Fernandez, Rafael Martin-Martin, Blanca Figuerola and Conxita Avila--
• 12. Metabolic and taxonomic diversity in Antarctic subglacial environments Trista J. Vick-Majors, Amanda M. Achberger, Alexander B. Michaud and John C. Priscu-- Part IV. Life and Habitability: Introduction:
• 13. Analytical astrobiology: the search for life signatures and the remote detection of biomarkers through their Raman spectral interrogation Howell G. M. Edwards and Jan Jehlicka--
• 14. Adaptation/acclimatization mechanisms of oxyphototrophic microorganisms and their relevance to astrobiology Jana Kviderova--
• 15. Life at the extremes Steven Chown.
• (source: Nielsen Book Data)

From deep ocean trenches and the geographical poles to outer space, organisms can be found living in remarkably extreme conditions. This book provides a captivating account of these systems and their extraordinary inhabitants, 'extremophiles'. A diverse, multidisciplinary group of experts discuss responses and adaptations to change; biodiversity, bioenergetic processes, and biotic and abiotic interactions; polar environments; and life and habitability, including searching for biosignatures in the extraterrestrial environment. The editors emphasize that understanding these systems is important for increasing our knowledge and utilizing their potential, but this remains an understudied area. Given the threat to these environments and their biota caused by climate change and human impact, this timely book also addresses the urgency to document these systems. It will help graduate students and researchers in conservation, marine biology, evolutionary biology, environmental change and astrobiology better understand how life exists in these environments and their susceptibility or resilience to change.
(source: Nielsen Book Data)

"From deep ocean trenches and the geographical poles to outer space, organisms can be found living in remarkably extreme conditions. This book provides a captivating account of these systems and their extraordinary inhabitants, 'extremophiles'. A diverse, multidisciplinary group of experts discuss responses and adaptations to change; biodiversity, bioenergetic processes, and biotic and abiotic interactions; polar environments; and life and habitability, including searching for biosignatures in the extraterrestrial environment. The editors emphasize that understanding these systems is important for increasing our knowledge and utilizing their potential, but this remains an understudied area. Given the threat to these environments and their biota caused by climate change and human impact, this timely book also addresses the urgency to document these systems. It will help graduate students and researchers in conservation, marine biology, evolutionary biology, environmental change and astrobiology better understand how life exists in these environments and their susceptibility or resilience to change"-- Provided by publisher.

• Part I: Detection, Quantification, and Confirmation
• 1. Traditional Methods of Analysis for Listeria monocytogenes
• Rui Magalhaes, Cristina Mena, Vania Ferreira, Goncalo Almeida, Joana Silva, and Paula Teixeira
• 2. MALDI-ToF MS: A Rapid Methodology for Identifying and Subtyping Listeria monocytogenes
• Snehal R. Jadhav, Rohan M. Shah, and Enzo A. Palombo
• 3. Sample Preparation for qPCR Detection of Listeria from Food
• Patrick Mester, Anna Kristina Witte, and Peter Rossmanith
• 4. qPCR Validation on the Basis of the Listeria monocytogenes prfA Assay
• Anna Kristina Witte, Patrick Mester, and Peter Rossmanith
• Part II: Subtyping Approaches
• 5. Serotype Assignment by Sero-Agglutination, ELISA, and PCR
• Lisa Gorski
• 6. Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes
• Karen Hunt and Kieran Jordan
• 7. Multi-Locus Sequence Typing (MLST) and Whole Genome Sequencing (WGS) of Listeria monocytogenes and Listeria innocua
• Beatrix Stessl, Martin Wagner, and Werner Ruppitsch
• Part III: Genotypic and Phenotypic Characterization
• 8. High-Throughput Characterization of Listeria monocytogenes Using the OmniLog Phenotypic Microarray
• Laura Luque-Sastre, Kieran Jordan, Seamus Fanning, and Edward M. Fox
• 9. High-Throughput Screening of Biofilm Formation of Listeria monocytogenes on Stainless Steel Coupons Using a 96-Well Plate Format
• Jessica A. Gray, P. Scott Chandry, John P. Bowman, and Edward M. Fox
• 10. Confocal Laser Microscopy Analysis of Listeria monocytogenes Biofilms and Spatially Organized Communities
• Maud Darsonval, Marina Gregoire, Julien Deschamps, and Romain Briandet
• 11. Extraction and Preparation of Listeria monocytogenes Subproteomes for Mass Spectrometry Analysis
• Tiago Santos and Michel Hebraud
• Part IV: Strain Manipulation
• 12. Extraction and Analysis of Plasmid DNA from Listeria monocytogenes
• Aidan Casey and Olivia McAuliffe
• 13. Generation of Nonpolar Deletion Mutants in Listeria monocytogenes Using the "SOEing" Method
• Kathrin Rychli, Eva Harter, Caitriona M. Guinane, Karen Daly, Colin Hill, and Paul D. Cotter
• 14. Mutant Construction and Integration Vector-Mediated Genetic Complementation in Listeria monocytogenes
• Cameron Parsons, Reha Azizoglu, Driss Elhanafi, and Sophia Kathariou
• Part V: Host-Pathogen Interactions
• 15. Internalization Assays for Listeria monocytogenes
• Andreas Kuhbacher, Pascale Cossart, and Javier Pizarro-Cerda
• 16. Microscopy of Intracellular Listeria monocytogenes in Epithelial Cells
• Helene Bierne, Mounia Kortebi, and Natalie Descoeudres
• Part VI: Control Methods
• 17. Control of Listeria monocytogenes Biofilms in a Simulated Food Processing Environment
• Sofia V. Poimenidou, Stavros G. Manios, and Panagiotis N. Skandamis
• 18. Sampling the Food Processing Environment: Taking up the Cudgel for Preventive Quality Management in Food Processing (FP)
• Martin Wagner and Beatrix Stessl
• 19. Isolation and Evaluation of Anti-Listeria Lactococcus lactis from Vegetal Sources
• Van Thi Thuy Ho, Anran Dong, Raquel Lo, and Mark S. Turner.
• (source: Nielsen Book Data)

This updated book explores a wide repertoire of tools and approaches that have been created, modified, and applied to the study of L. monocytogenes, forming the basis of our understanding of the bacterium today. Many of these key experimental techniques are gathered together herein. The volume presents aspects such as clinical disease and host-pathogen interactions, as well as the study of biofilms which present a significant challenge for control of the organism in the food processing environment. The topics covered in this edition also include sampling in order to isolate Listeria, methods for their identification and characterization, methods for gene manipulation, and methods for control of the organism. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Listeria monocytogenes: Methods and Protocols, Second Edition aims to contribute toward the harmonization of methods used to study this important bacterium, and to be of particular interest to Listeria research both in relation to food association and control as well as clinical microbiology.
(source: Nielsen Book Data)

• Bacterial Endophytes: Diversity, Functional Importance, and Potential for Manipulation.
• Rhizosphere Carbon Turnover from Cradle to Grave: The Role of Microbe-Plant Interactions.
• Root-Soil-Microbe Interactions Mediating Nutrient Fluxes in the Rhizosphere.
• Diazotrophic Nitrogen Fixation in the Rhizosphere and Endosphere.
• Root Microbiome Structure and Microbial Succession in the Rhizosphere.
• Rhizosphere Legacy: Plant Root Interactions with the Soil and Its Biome.
• Rhizosphere Microbiome and Soil-Borne Diseases.
• Root Disease Impacts on Root-Rhizosphere Microbial Communities.
• Newly Introduced or Modified Genes in Plants Potentially Modulate the Host Microbiome.
• Rhizosphere Plant-Microbe Interactions Under Abiotic Stress.
• Arbuscular Mycorrhizal Fungi Interactions in the Rhizosphere.
• Microbial-Faunal Interactions in the Rhizosphere.
• Inter-Organismal Signaling in the Rhizosphere.
• Molecular Mechanisms of Plant-Microbe Interactions in the Rhizosphere as Targets for Improving Plant Productivity.
• Inoculation Effects in the Rhizosphere: Diversity and Function.

This book presents a detailed discussion on the direct interactions of plants and microorganisms in the rhizosphere environment. It includes fifteen chapters, each focusing on a specific component of plant-microbe interactions, such as the influence of plants on the root microbiome, and the downstream effects of rhizosphere microbial dynamics on carbon and nutrient fluxes in the surroundings. As such, the book helps readers gain a better understanding of diversity above the ground, and its effect on the microbiome and its functionality.

• 1. Next day Salmonella spp. Detection Method Based on real-time PCR for Foods
• David Rodriguez-Lazaro and Marta Hernandez
• 2. Isolation of Salmonella spp. from Animal Feed
• KM Feye, AE Powell, Blaine Booher, Zachary Flores, PM Rubinelli, LH Calderwood, KE Richardson, PA Davis, R Sellers, and SC Ricke
• 3. Investigating Outbreaks of Salmonella Typhimurium using Case-control Studies, with a Reference to the One Health Approach
• Katrin Gaardbo Kuhn and Steen Ethelberg
• 4. Detection of Salmonella by the 3M Molecular Detection Assays - MDS (R) Method
• Greici Bergamo and Eliezer Avila Gandra
• 5. CRISPR-Typing of Salmonella Isolates
• Nikki Shariat and Edward Dudley
• 6. CRISPR Typing of Salmonella Isolates from Different Resources
• Qiuchun Li
• 7. Immunomagnetic Separation of Salmonella with Tailored Magnetic Micro and Nanocarriers
• Maria Isabel Pividori
• 8. Self-labeling Enzyme Tags for Translocation Analyses of Salmonella Effector Proteins Vera Goeser and Michael Hensel
• 9. Smartphone Based Paper Microfluidic Immunoassay of Salmonella and E. coli Laurel Dieckhaus, Tu San Park, and Jeong-Yeol Yoon
• 10. Correlative Light and Scanning Electron Microscopy to Study Interactions of Salmonella enterica with Polarized Epithelial Cell Monolayers Carina Kommnick and Michael Hensel
• 11. Production of Murine Macrophages from Hoxb8-immortalized Myeloblasts: Utility and use in the Context of Salmonella Infection
• Ziyan Fang, Margaux Lagier, and Stephane Meresse
• 12. In vitro Evaluation of Anti-biofilm Agents Against Salmonella enterica
• Jenna Sandala and John S. Gunn
• 13. Generation of Random luxCDABE Transcripcional Fusions in the Genome of Salmonella enterica
• Juan J. de la Rosa-Altura, Barbora Benesova, Concepcion Panadero-Medianero, Aida Amador-Alvarez, Julia Aguilera-Herce, Juan Luis Araujo-Garrido, Joaquin Bernal-Bayard, and Francisco Ramos-Morales
• 14. Vaccine Based On Outer Membrane Vesicles Using Hydrogels as Vaccine Delivery System
• Yadira Pastor, Isaiah Ting, Melibea Berzosa, Juan M. Irache, and Carlos Gamazo
• 15. Detection and Characterization of Salmonella enterica Serotypes by Simple PCR Technologies
• Samuel Kariuki and John Kiiru
• 16. Determination of Antimicrobial Resistance of Salmonella in Pork
• Jing Wang and Dan Gu
• 17. Molecular typing of Salmonella by Pulsed-field Gel Electrophoresis
• Xilong Kang, Chuang Meng, and Zhiming Pan
• 18. Isolation and Identification of Salmonella in Pork
• Hongqin Song and Aihua Zhu.
• (source: Nielsen Book Data)

This third edition expands upon the previous edition with current, detailed methods on different aspects, and landmarks advancing knowledge on Salmonella research. With new chapters on molecular assays for detection, identification and serotyping of Salmonella, quantitative proteomic identification of host factors involved in salmonella infection, determination of antimicrobial resistance in salmonella, site-directed mutagenesis, chromosomal gene analysis, various microscopy methods to analyze salmonella interaction with host cells, in vitro modeling of gallbladder-associated salmonella colonization, and analysis of salmonella phages and prophages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Salmonella: Methods and Protocols, Third Edition aims to provide methods that will have an impacted Salmonella research.
(source: Nielsen Book Data)

• 1. History of antimicrobial resistance
• 2. Use of antimicrobials and antibiotics in livestock, poultry, fishery and agriculture
• 3. The emergence of antimicrobialresistant bacteria in livestock, poultry and agriculture
• 4. Emergence of antimicrobial resistant bacteria in aquaculture
• 5. Emergence of antimicrobial-resistant bacteria in environment
• 6. ss-Lactamase
• 7. Carbapenem resistance
• 8. Resistance to fluoroquinolones
• 9. Resistance to aminoglycoside, tetracycline and macrolides
• 10. Colistin resistance
• 11. Antifungal resistance
• 12. Biofilm formation and persister cells
• 13. Salmonella
• 14. Klebsiella
• 15. Escherichia coli
• 16. Staphylococcus
• 17. Streptococcus
• 18. Actinobacillus
• 19. Campylobacter
• 20. Clostridium
• 21. Pasteurella and Mannheimia
• 22. Vibrio
• 23. Pseudomonas
• 24. Aeromonas
• 25. Mycobacterium
• 26. Candida
• 27. Others
• 28. Cross-resistance between biocides and antimicrobials
• 29. Antimicrobial stewardship
• 30. Alternative antiinfective therapy
• 31. Immunotherapy
• 32. Antimicrobial resistance: one health approach.
• (source: Nielsen Book Data)

Antimicrobial Resistance in Agriculture: Perspective, Policy and Mitigation is a valuable industrial resource that addresses complex, multi-factorial topics regarding farm, wild, companion animals, fish, and how the environment plays an important role in amplification and transmission of resistant bugs into the human food chain. Information of phenotypical and genotypical properties of each bacterial genus associated with antimicrobial resistance, transmission dynamics from different reservoirs (food animals, poultry, fishes) and control measures with alternative therapy, such as phytobiotics and nanomaterials are provided. Researchers, scientists and practitioners will find this an essential resource on the judicial use of antibiotics in animals and humans.
(source: Nielsen Book Data)

• B cell development and maturation.- B cell receptor signalling.- Involvement of reactive oxygen species (ROS) in BCR signaling as a second messenger.- Germinal center reaction.- Memory B cells in local and systemic sites.- Regulation of plasma cell differentiation.- Regulation of humoral immune responses and B cell tolerance by the IgM Fc receptor.- Regulatory B cells.- Diversified IgA-bacteria interaction in gut homeostasis.- Primary antibody deficiencies.- B cell-mediated autoimmune diseases.- B cell lymphomas.
• (source: Nielsen Book Data)

This book contains twelve chapters contributed by prestigious international experts who are at the forefront of B cell research, and aims to provide a cutting-edge and comprehensive overview of all aspects of B cells, including B cell development, maturation and activation, germinal center reaction, memory and plasma cell differentiation, and antibody-mediated positive and negative regulation of humoral immune responses. There are also three chapters describing human diseases caused by B cell abnormalities, including primary antibody deficiencies, autoimmune diseases, and B cell malignancies. We hope that this book will become a standard and routine reference for both basic researchers and clinicians.
(source: Nielsen Book Data)

• Contributors ix
• Preface xv
• About the Editors xvii
• I. Cellular microbiology in the study of tissue and organ infections
• 1. Interaction between Intracellular Bacterial Pathogens and Host Cell Mitochondria 3 Anna Spier, Fabrizia Stavru, and Pascale Cossart
• 2 Shigella Pathogenesis: New Insights through Advanced Methodologies 15 Pamela Schnupf and Philippe J. Sansonetti
• 3. The Interplay between Salmonella enterica Serovar Typhimurium and the Intestinal Mucosa during Oral Infection 41 Annika Hausmann and Wolf-Dietrich Hardt
• 4. New Age Strategies To Reconstruct Mucosal Tissue Colonization and Growth in Cell Culture Systems 59 Alyssa C. Fasciano, Joan Mecsas, and Ralph R. Isberg
• 5. The Many Faces of Bacterium-Endothelium Interactions during Systemic Infections 69 Dorian Obino and Guillaume Dumenil
• 6. Reaching the End of the Line: Urinary Tract Infections 83 Kevin O. Tamadonfar, Natalie S. Omattage, Caitlin N. Spaulding, and Scott J. Hultgren
• 7. The Intracellular Life Cycle of Brucella spp. 101 Jean Celli
• 8. Infect and Inject: How Mycobacterium tuberculosis Exploits Its Major Virulence-Associated Type VII Secretion System, ESX-1 113 Sangeeta Tiwari, Rosalyn Casey, Celia W. Goulding, Suzie Hingley-Wilson, and William R. Jacobs, JR.
• 9. Mycobacterium tuberculosis: Bacterial Fitness within the Host Macrophage 127 Lu Huang, Evgeniya V. Nazarova, and David G. Russell
• 10. The Wolbachia Endosymbionts 139 Frederic Landmann
• 11. Make It a Sweet Home: Responses of Chlamydia trachomatis to the Challenges of an Intravacuolar Lifestyle 155 Sebastien Triboulet and Agathe Subtil
• 12. Salmonella Single-Cell Metabolism and Stress Responses in Complex Host Tissues 167 Dirk Bumann
• 13. Manipulation of Host Cell Organelles by Intracellular Pathogens 179 Titilayo O. Omotade and Craig R. Roy
• II. Subcellular microbiology
• 14. The Role of the Type III Secretion System in the Intracellular Lifestyle of Enteric Pathogens 199 Marcela de Souza Santos and Kim Orth
• 15. Customizing Host Chromatin: A Bacterial Tale 215 Michael Connor, Laurence Arbibe, and Melanie Hamon
• 16. Cell Biology of Intracellular Adaptation of Mycobacterium leprae in the Peripheral Nervous System 227 Samuel Hess and Anura Rambukkana
• 17. Multifaceted Roles of MicroRNAs in Host-Bacterial Pathogen Interaction 247 Carmen Aguilar, Miguel Mano, and Ana Eulalio
• 18. Modulation of Host Cell Metabolism by Chlamydia trachomatis 267 Marion Rother, Ana Rita Teixeira da Costa, Rike Zietlow, Thomas F. Meyer, and Thomas Rudel
• III. Autonomous defense pathways in the cell
• 19. Host-Encoded Sensors of Bacteria: Our Windows into the Microbial World 279 Charlotte Odendall and Jonathan C. Kagan
• 20. Recognition of Intracellular Bacteria by Inflammasomes 287 Petr Broz
• IV. New technologies to move cellular microbiology to organs and tissues
• 21. Modeling Infectious Diseases in Mice with a "Humanized" Immune System 301 Yan Li and James P. di Santo
• 22. A Cinematic View of Tissue Microbiology in the Live Infected Host 315 Agneta Richter-Dahlfors and Keira Melican
• 23. Cellular Imaging of Intracellular Bacterial Pathogens 325 Virginie Stevenin and Jost Enninga
• 24. Using a Systems Biology Approach To Study Host-Pathogen Interactions 337 Amy Yeung, Christine Hale, Simon Clare, Sophie Palmer, Josefin Bartholdson Scott, Stephen Baker, and Gordon Dougan
• Index 349.
• (source: Nielsen Book Data)

Bacteria and Intracellularity clearly demonstrates that cellular microbiology as a field has reached maturity, extending beyond the strictly cellular level to infections of various organs and tissues. Decades of intense investigation into host-bacterial pathogen interactions have highlighted common concepts in intracellularity but also very diverse mechanisms underlying the various infections produced by bacteria. This book offers a wide-ranging look at the latest studies, including: foodborne pathogens, including how, when, and where bacteria interact with the gut and its microbiota infections of the urogenital tract, endothelial barriers, and the nervous system major advances in work with Mycobacterium tuberculosis and M. leprae subcellular microbiology, including metabolism of infected cells, nuclear biology, and microRNAs endosymbionts, in particular the latest work with Wolbachia and its effect on insect transmission of viral pathogens research into cell autonomous defense pathways that has led to major insights into immunology and innate immunity the latest developments in technology, for the next steps in the study of intracellularity All facets of cellular physiology, within the entire scope of cells and host tissues, can be targeted by pathogens. This book offers to researchers, students, and laboratorians a valuable overview of the state of current research into the cellular microbiology of host-pathogen interactions.
(source: Nielsen Book Data)

• 1. Mycorrhiza: A Potential Bio-Enhancer in Agriculture Production Systems
• Bakulranjan Jana
• 2. Plant Growth Promoting Microbiome Networks
• OEzlem Akkaya, Mine Gul Seker, and Yelda OEzden Ciftci
• 3. Plant Growth-Promoting Endophytic Bacteria and Their Potential Benefits in Asian Countries
• Nittaya Pitiwittayakul and Somboon Tanasupawat
• 4. Eccentricity in the Behavior of Pencillium Spp. as Phytopathogen and Phytoaugmentor
• Dhaval Patel et al.
• 5. Environmental Management of E-Waste by Biological Process
• Aparna Gunjal et al.
• 6. Microbial Degradation of Wastes for Environmental Protection
• Manobendro Sarker and Md. Maksudur Rahman
• 7. Soil Microbial Biofilm Communities and Their Interactions
• Bindu Sadanandan, Priya Ashrit, and V. Vijayalakshmi
• 8. Bacteriological Removal of Azo Dyes: An Eco-Friendly Approach
• Shanmugapriya Saravanabhavan et al.
• 9. Impact of Soil-Plant Microbe Interaction in Metal-Contaminated Soils
• Neetu Sharma, Abhinashi Singh, and Navneet Batra
• 10. Radiation-Resistant Thermophiles: From High Temperature and Radiation to Engineered Bioremediation
• Preeti Ranawat and Seema Rawat
• 11. Dynamic Potential of Indigenous and Effective Microbes in Wastewater Treatment Processes
• Jeyabalan Sangeetha et al.
• 12. Earthworms and Microbes in Environmental Management through Vermitechnology-Mediated Organic Farming
• Abdullah Adil Ansari.
• (source: Nielsen Book Data)

This new volume, Beneficial Microbes for Sustainable Agriculture and Environmental Management, reviews the multifaceted interactions among microbes, ecosystems, and their pivotal role in maintaining a more balanced environment, in order to help facilitate living organisms coexisting with the natural environment. Microbes are the most abundant organisms in the biosphere and regulate many critical elemental and biogeochemical phenomenona. Because microbes are the key players in the carbon cycle and in related biological reactions, microbial ecology is a vital research area for understanding the contribution of the biosphere in global warming and the response of the natural environment to climate variations. The beneficial uses of microbes have enabled constructive and cost-effective responses that have not been possible through physical or chemical methods. This book provides valuable information on microbial utilization for environmental sustainability and provides fascinating insights into microbial diversity.
(source: Nielsen Book Data)
Microbes are the most abundant organisms in the biosphere and regulate many critical elemental and biogeochemical phenomena. Because microbes are the key players in the carbon cycle and in related biological reactions, microbial ecology is a vital research area for understanding the contribution of the biosphere in global warming and the response of the natural environment to climate variations. The beneficial uses of microbes have enabled constructive and cost-effective responses that have not been possible through physical or chemical methods. This new volume reviews the multifaceted interactions among microbes, ecosystems, and their pivotal role in maintaining a more balanced environment, in order to help facilitate living organisms coexisting with the natural environment. With extensive references, tables, and illustrations, this book provides valuable information on microbial utilization for environmental sustainability and provides fascinating insights into microbial diversity. Key features include: Looks at enhancing plant production through growth-promoting arbuscular mycorrhizae, endophytic bacteria, and microbiome networks Considers microbial degradation and environmental management of e-wastes and azo dyes Explores soil-plant microbe interactions in metal-contaminated soils Examines radiation-resistant thermophiles for engineered bioremediation Describes potential indigenous/effective microbes for wastewater treatment processes Presents research on earthworms and microbes for organic farming.
(source: Nielsen Book Data)

• Introduction
• When to refer a patient for CAR-T cell therapy
• Optimizing the apheresis product
• CAR T cells: cell processing laboratory considerations
• Peri-CAR-T cell management
• Management of cytokine release syndrome
• Special considerations for ICU management of patients receiving CAR therapy
• Neurotoxicities after CAR-T cell immunotherapy
• Hematologic and non-CRS toxicities
• Response assessment and post-CAR-T cell therapy management
• Relapse management and role for consolidative hematopoietic stem cell transplantation following CAR-T cell therapy
• Promising chimeric antigen receptors for non-B cell hematological malignancies, pediatric solid tumors, and carcinomas
• CAR T cell therapy for CNS malignancies
• CAR 2.0: the next generation of synthetic receptor-based cellular therapy for cancer.

• Introduction
• 1. When to Refer a Patient for CAR-T Cell Therapy
• 2. Optimizing the Apheresis Product
• 3. CAR T Cells: Cell Processing Laboratory Considerations
• 4. Peri-CAR-T Cell Management
• 5. Management of Cytokine Release Syndrome
• 6. Special Considerations for ICU Management of Patients Receiving CAR Therapy
• 7. Neurotoxicities After CAR-T Cell Immunotherapy
• 8. Hematologic and Non-CRS Toxicities
• 9. Response Assessment and PosteCAR-T Cell Therapy Management
• 10. Relapse Management and Role for Consolidative Hematopoietic Stem Cell Transplantation Following CAR-T Cell Therapy
• 11. Promising Chimeric Antigen Receptors for Non-B Cell Hematological Malignancies, Pediatric Solid Tumors, and Carcinomas
• 12. CAR T Cell Therapy for CNS Malignancies
• 13. CAR 2.0: The Next Generation of Synthetic ReceptoreBased Cellular Therapy for Cancer.
• (source: Nielsen Book Data)

Chimeric Antigen Receptor (CAR) T-Cell Therapies for Cancer: A Practical Guide presents a comprehensive view of CAR modified T-cells from patient referral to post-therapy management in a concise and practical format. Providing clinical guidance on the implementation and management of CAR T-cell therapy, this clinical resource keeps you up-to-date on the latest developments in this rapidly evolving area. Covers all clinical aspects, including patient referral, toxicities management, comorbidities, bridging therapy, post-CAR monitoring, and multidisciplinary approaches to supportive care. Includes key topics on associated toxicities such as predictive biomarkers, infections, and multidisciplinary approaches to supportive care. Presents current knowledge on FDA approved CAR T-cell products as well as developments on the horizon. Editors and authors represent leading investigators in academia and worldwide pioneers of CAR therapy.
(source: Nielsen Book Data)

• Introduction - taking xenotransplantation into the clinic
• 1. Xenotransplantation
• in nonhuman primates - the present position
• Kidney xenotransplantation
• Heart xenotransplantation
• 2.
• Donor genetics and potential infectious risks
• The optimal genetically-engineered organ-source pig
• Organ-source pig health status and potential infectious risks
• Regulatory requirements for the organ-source pig
• 3.
• Antibody-mediated allotransplant rejection: lessons for xenotransplantation
• The problem of anti-HLA antibodies
• The problem of ABO blood group-incompatibility
• 4. Patient
• evaluation and selection for first clinical trials
• Kidney transplantation
• Heart transplantation in adults
• Heart transplantation in infants and children
• Heart transplantation vs mechanical circulatory support
• 5.
• Social and religious aspects of xenotransplantation
• 6.
• Economic implications
• of xenotransplantation
• 7. Summary - closing in on clinical xenotransplantation.
• (source: Nielsen Book Data)

This title provides an illuminating examination of the current state of xenotransplantation - grafting or transplanting organs or tissues between members of different species - and how it might move forward into the clinic. To be sure, this is a critical topic, as a major problem that remains worldwide is an inadequate supply of organs from deceased human donors, severely limiting the number of organ transplants that can be performed each year. Based on presentations given at a major conference on xenotransplantation, this title includes important views from many leading experts who were invited to present their data and opinions on how xenotransplantation can advance into the clinic. Attention was concentrated on pig kidney and heart transplantation as it is in regard to these organs that most progress has been made. Collectively, these chapters effectively highlight the many advantages of xenotransplantation to patients with end-stage organ failure, thereby encouraging the mapping of a concrete pathway to clinical xenotransplantation. The book is organized across 22 chapters, beginning with background information on clinical and experimental xenotransplantation. Following this are discussions addressing how pigs can be genetically engineered for their organs to be resistant to the human immune response through deletion of pig xenoantigens, and the insertion of 'protective' human transgenes. Subsequent chapters analyze complications that arise in practice, comparing allotransplant and xenotransplant rejection. The selection of the ideal patients for the first clinical trials is discussed. Finally, the book concludes with an analysis on the regulatory, economic, and social aspects of this research, including FDA perspectives and the sensitive, psychosocial factors regarding allotransplantation and xenotransplantation. A major and timely addition to the literature, Clinical Xenotransplantation will be of great interest to all researchers, physicians, and academics from other disciplines with an interest in xenotransplantation.
(source: Nielsen Book Data)

• Preface ix
• Acknowledgments xi
• Technical Note xi
• About the Authors xiii
• 1 Staphylococcus, Micrococcus, and Other Catalase?Positive Cocci 1
• 2 Streptococcus 11
• 3 Enterococcus 24
• 4 Aerococcus, Abiotrophia, and Other Miscellaneous Gram-Positive Cocci That Grow Aerobically 30
• 5 Coryneform Gram-Positive Bacilli 36
• 6 Listeria and Erysipelothrix 48
• 7 Bacillus 54
• 8 Nocardia, Rhodococcus, Actinomadura, Streptomyces, Gordonia, and Other Aerobic Actinomycetes 62
• 9 Mycobacterium 70
• 10 Introduction to Enterobacterales 91
• 11 Escherichia, Shigella, and Salmonella 103
• 12 Klebsiella, Enterobacter, Citrobacter, Cronobacter, Serratia, Plesiomonas, and Selected Other Enterobacterales 113
• 13 Yersinia 129
• 14 Vibrionaceae 134
• 15 Aeromonas 141
• 16 Pseudomonas 145
• 17 Burkholderia, Stenotrophomonas, Ralstonia, Cupriavidus, Pandoraea, Brevundimonas, Comamonas, Delftia, and Acidovorax 150
• 18 Acinetobacter, Chryseobacterium, Moraxella, Methylobacterium, and Other Nonfermentative Gram-Negative Bacilli 157
• 19 Actinobacillus, Aggregatibacter, Capnocytophaga, Eikenella, Kingella, Pasteurella, and Other Fastidious or Rarely Encountered Gram-Negative Bacilli 168
• 20 Legionella 180
• 21 Neisseria 184
• 22 Haemophilus 191
• 23 Bordetella and Related Genera 197
• 24 Brucella 203
• 25 Bartonella 207
• 26 Francisella 210
• 27 Introduction to Anaerobic Bacteria 213
• 28 Clostridium and Clostridioides 223
• 29 Peptostreptococcus, Finegoldia, Anaerococcus, Peptoniphilus, Cutibacterium, Lactobacillus, Actinomyces, and Other Non-Spore-Forming, Anaerobic Gram-Positive Bacteria 237
• 30 Bacteroides, Porphyromonas, Prevotella, Fusobacterium, and Other Anaerobic Gram-Negative Bacteria 252
• 31 Campylobacter and Arcobacter 261
• 32 Helicobacter 267
• 33 Chlamydia 272
• 34 Mycoplasma and Ureaplasma 277
• 35 Leptospira, Borrelia, Treponema, and Brachyspira 281
• 36 Rickettsia, Orientia, Ehrlichia, Anaplasma, and Coxiella 290
• 37 Tropheryma whipplei 297
• 38 Antimicrobial Susceptibility Testing 299
• 39 Molecular Diagnosis of Bacterial Infections 307
• 40 Total Laboratory Automation 330
• 41 Stains, Media, Reagents, and Histopathology 338
• 42 Fast Facts: Bacteria 367
• Index 421.
• (source: Nielsen Book Data)

This unique visual reference presents more than 750 brilliant, four-color images of bacterial isolates commonly encountered in diagnostic microbiology and the methods used to identify them, including microscopic and phenotypic characteristics, colony morphology, and biochemical properties. Chapters cover the most important bacterial pathogens and related organisms, including updated taxonomy, epidemiology, pathogenicity, laboratory and antibiotic susceptibility testing, and molecular biology methodology Tables summarize and compare key biochemical reactions and other significant characteristics New to this edition is a separate chapter covering the latest developments in total laboratory automation The comprehensive chapter on stains, media, and reagents is now augmented with histopathology images A new Fast Facts chapter presents tables that summarize and illustrate the most significant details for some of the more commonly encountered organisms For the first time, this easy-to-use atlas is available digitally for enhanced searching. Color Atlas of Medical Bacteriology remains the most valuable illustrative supplement for lectures and laboratory presentations, as well as for laboratorians, clinicians, students, and anyone interested in diagnostic medical bacteriology.
(source: Nielsen Book Data)