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Peroxisome proliferator-activated receptors (PPARs) : methods and protocols / edited by Mostafa Z. Badr, Jihan Youssef.

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Language:
English.
Publication date:
2013
Imprint:
New York : Humana Press, c2013.
Format:
  • Book
  • xi, 343 p. : ill. (some col.) ; 26 cm.
Bibliography:
Includes bibliographical references and index.
Contents:
  • PPARs : history and advances / Jihan Youssef and Mostafa Z. Badr
  • PPAR-alpha cloning, expression, and characterization / Suong N.T. Ngo and Ross A. McKinnon
  • PPARGC1A and PPARG genotyping : beginner's guide to genotyping with unlabeled probes / Viera Habalova and Jozef Židzik
  • Generation of an inducible, cardiomyocyte-specific transgenic mouse model with PPAR [beta]/[delta] overexpression / Teayoun Kim ... [et al.]
  • Specific knockdown of PPAR[delta] gene in colon cancer cells by lentivirus-mediated RNA Interfering / Lie Yang, Zong-Guang Zhou, and Xiao-Feng Sun
  • Dominant-negative and knockdown approaches to studying PPAR activity / Sudhir Singh and Robert G. Bennett
  • Producing PPARgamma2 knockdown in mouse liver / Tomomi Yamazaki and Osamu Ezaki
  • Adipose tissue-specific PPAR[gamma] gene targeting / Weimin He
  • Site-directed mutagenesis to study the role of specific amino acids in the ligand binding domain of PPARs / Nico Mitro ... [et al.]
  • PPAR SUMOylation : some useful experimental tips / Benoit Pourcet, Bart Staels, and Corine Glineur
  • Analyzing phosphorylation-dependent regulation of subcellular localization and transcriptional activity of transcriptional coactivator NT-PGC-1[alpha] / Ji Suk Chang and Thomas W. Gettys
  • In vivo studies of PPAR-chromatin interactions : chromatin immunoprecipitation for single-locus and genomewide analyses / Till Adhikary and Rolf Müller
  • FISH analysis using PPAR[gamma]-specific probes for detection of PAX8-PPAR[gamma] translocation in follicular thyroid neoplasms / Noor Akmal Syed Husain Sharifah, Zubaidah Zakaria, and Wai Kit Chia
  • Immunohistochemical techniques to identify and localize proteins of interest in paraffin embedded tissue sections / Carolyn M. Komar and Mary Jane Long
  • Determination of PPAR expression by western blot / Aziz Guellich and Catherine Coirault
  • Fluorescence resonance energy transfer techniques to study ligand-mediated interactions of PPARs with coregulators / Nico Mitro, Cristina Godio, and Maurizio Crestani
  • Estimation of the PPAR[alpha] agonism of fibrates by a combined MM-docking approach / Fabio Lannutti, Alessandro Marrone, and Nazzareno Re
  • Combined biophysical and cell-based approaches for the assessment of ligand binding to PPAR[gamma] / Silvia Zorrilla and Dolores Pérez-Sala
  • Exploring PPAR modulation in experimental mice / Anna Rull ... [et al.]
  • Induction of adipogenic differentiation in three-dimensional culture model on a novel microfabricated scaffold / Yoshitaka Miyagawa, Hajime Okita, and Nobutaka Kiyokawa
  • Analyzing PPAR[alpha]/ligand interactions by chemical cross-linking and high-resolution mass spectrometry / Mathias Q. Müller, Christian H. Ihling, and Andrea Sinz
  • Synthesis, mass spectrometric characterization, and analysis of the PPAR[delta] agonist GW1516 and its major human metabolites : targets in sports drug testing / Mario Thevis ... [et al.]
  • LC-MS-based method for the qualitative and quantitative analysis of the novel PPAR[gamma] agonist KR-62980 / Min-Sun Kim and Myung Ae Bae
  • Behavioral paradigms to evaluate PPAR modulation in animal models of brain injury / Dana Greene-Schloesser, Caroline I. Schnegg, and Mike E. Robbins.
Contributor:
Badr, Mostafa Z., 1950-
Youssef, Jihan A., 1959-
Series:
Methods in molecular biology, 1064-3745 ; 952
Springer protocols
Methods in molecular biology (Clifton, N.J.) v. 952.
Springer protocols (Series)
Subjects:
ISBN:
9781627031547
1627031545

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