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Nanotechnology for nucleic acid delivery : methods and protocols / edited by Manfred Ogris, David Oupicky.

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Language:
English.
Publication date:
2013
Imprint:
New York : Humana Press, c2013.
Format:
  • Book
  • xii, 286 p. : ill. (some color) ; 26 cm.
Bibliography:
Includes bibliographical references and index.
Contents:
  • Toxicological aspects for nanomaterial in humans
  • Lactate dehydrogenase assay for assessment of polycation cytotoxicity
  • Combined fluorimetric caspase 3/7 assay and bradford protein determination for assessment of polycation-mediated cytotoxicity
  • Anti-PEG igm production via a PEGylated nano-carrier system for nucleic acid delivery
  • Near-infrared optical imaging of nucleic acid nanocarriers in vivo
  • Lipids for nucleic acid delivery: Synthesis and particle formation
  • Histidine-rich cationic amphipathic peptides for plasmid DNA and siRNA delivery
  • Synthesis of polyethylenimine-based nanocarriers for systemic tumor targeting of nucleic acids
  • Synthesis of bioreducible polycations with controlled topologies
  • Lyophilization of synthetic gene carriers
  • Surface- and hydrogel-mediated delivery of nucleic acid nanoparticles
  • Layer-by-layer assembled gold nanoparticles for the delivery of nucleic acids
  • In situ AFM analysis investigating disassembly of DNA nanoparticles and nano-films
  • Enhancing nucleic acid delivery with ultrasound and microbubbles
  • Magnetic and acoustically active microbubbles loaded with nucleic acids for gene delivery
  • Synthesis of lipidic magnetic nanoparticles for nucleic acid delivery
  • Lipopeptide delivery of siRNA to the central nervous system
  • Flow cytometry-based cell type-specific assessment of target regulation by pulmonary siRNA delivery
  • Liver-targeted gene delivery through retrograde intrabiliary infusion.
Contributor:
Ogris, Manfred.
Oupicky, David.
Series:
Methods in molecular biology, 1064-3745 ; 948
Springer protocols
Methods in molecular biology (Clifton, N.J.) v. 948.
Springer protocols (Series)
Subjects:
ISBN:
9781627031394
1627031391
9781627031400
1627031405

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