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Fungal secondary metabolism : methods and protocols / edited by Nancy P. Keller, Geoffrey Turner.

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Language:
English.
Publication date:
2012
Imprint:
New York : Humana Press, c2012.
Format:
  • Book
  • xii, 288 p. : ill. (some col.) ; 26 cm.
Bibliography:
Includes bibliographical references and index.
Contents:
  • Library preparation and data analysis packages for rapid genome sequencing
  • Bioinformatics approaches and software for detection of secondary metabolic gene clusters
  • Media and growth conditions for induction of secondary metabolite production
  • Manipulating filamentous fungus chemical phenotypes by growth on nutritional arrays
  • The "FERMEX" Method for metabolite-enriched fungal extracts
  • The chemical identification and analysis of aspergillus nidulans secondary metabolites
  • Detection and purification of non-ribosomal peptide synthetase products in Neosartorya fischeri
  • Production, detection, and purification of clavine-type ergot alkaloids
  • Analysis of volatile compounds emitted by filamentous fungi using solid-phase microextraction-gas chromatography/mass spectrometry
  • Tools for manipulation of secondary metabolism pathways: Rapid promoter replacements and gene deletions in Aspergillus nidulans
  • Fast and easy method for construction of plasmid vectors using modified quick-change mutagenesis
  • Reconstitution of a secondary metabolite biosynthetic pathway in a heterologous fungal host
  • Multi-copy genetic screen in Aspergillus nidulans
  • Identification of protein complexes from filamentous fungi with tandem affinity purification
  • Correlating secondary metabolite production with genetic changes using differential analysis of 2d NMR spectra
  • Chromatin immunoprecipitation analysis in filamentous fungi
  • Targeted proteomics for metabolic pathway optimization
  • Investigation of in vivo protein interactions in Aspergillus spores
  • Purification of a vesicle-vacuole (v) fraction from Aspergillus
  • Use of the hollow fiber assay for the discovery of novel anticancer agents from fungi
  • Molecular analysis of fungal gene expression upon interkingdom competition with insects.
Contributor:
Keller, Nancy P. (Nancy Pulane), 1955-
Turner, Geoffrey (Molecular biologist)
Series:
Methods in molecular biology, 1064-3745 ; 944
Springer protocols
Methods in molecular biology (Clifton, N.J.) v.944.
Springer protocols (Series)
Subjects:
ISBN:
9781627031219
1627031219
9781627031226
1627031227

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