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siRNA design : methods and protocols / edited by Debra J. Taxman.

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Language:
English.
Publication date:
2013
Imprint:
New York : Humana Press, c2013.
Format:
  • Book
  • xiii, 392 p. : ill. (some col.) ; 26 cm.
Bibliography:
Includes bibliographical references and index.
Contents:
  • What parameters to consider and which software tools to use for target selection and molecular design of small interfering RNAs / Olga Matveeva
  • Methods for selecting effective siRNA target sequences using a variety of statistical and analytical techniques / Shigeru Takasaki
  • Designing functional siRNA with reduced off-target effects / Yuki Naito and Kumiko Ui-Tei
  • Design and screening of siRNAs against highly structured RNA targets / Neda Nasheri, John Paul Pezacki, and Selena M. Sagan
  • Engineering small interfering RNAs by strategic chemical modification / Jesper B. Bramsen and Jørgen Kjems
  • Design, selection, and evaluation of highly specific and functional siRNA incorporating unlocked nucleobase analogs / Narendra Vaish and Pinky Agarwal
  • Design, preparation and evaluation of asymmetric small interfering RNA for specific gene silencing in mammalian cells / Chanil Chang ... [et al.]
  • Design of nuclease-resistant fork-like small interfering RNA (fsiRNA) / Elena L. Chernolovskaya and Marina A. Zenkova
  • Designing dual-targeting siRNA duplexes having two active strands that combine siRNA and MicroRNA-like targeting / Pal Sætrom
  • Strategies for designing and Validating immunostimulatory siRNAs / Michael P. Gantier
  • Designing efficient and specific endoribonuclease-prepared siRNAs / Vineeth Surendranath ... [et al.]
  • Short hairpin RNA-mediated gene silencing / Luke S. Lambeth and Craig A. Smith
  • Design of lentivirally expressed siRNAs / Ying Poi Liu and Ben Berkhout
  • Bifunctional short hairpin RNA (bi-shRNA) : design and pathway to clinical application / Donald D. Rao ... [et al.]
  • Design and chemical modification of synthetic short shRNAs as potent RNAi triggers / Anne Dallas and Brian H. Johnston
  • Production and application of long dsRNA in mammalian cells / Katerina Chalupnikova, Jana Nejepinska, and Petr Svoboda
  • Design of RNAi reagents for invertebrate model organisms and human disease vectors / Thomas Horn and Michael Boutros
  • Construction of shRNA expression plasmids for silkworm cell lines using single-stranded DNA and Bst DNA polymerase / Hiromitsu Tanaka
  • Designing effective amiRNA and multimeric amiRNA against plant viruses / Muhammad Fahim and Philip J. Larkin
  • Downregulation of plant genes with miRNA-induced gene silencing / Felipe Fenselau de Felippes.
Contributor:
Taxman, Debra J.
Series:
Methods in molecular biology, 1064-3745 ; 942
Springer protocols, 1949-2448
Methods in molecular biology (Clifton, N.J.) v. 942.
Springer protocols (Series)
Subjects:
ISBN:
9781627031189
1627031189

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