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Germline development : methods and protocols / edited by Wai-Yee Chan, Le Ann Blomberg.

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Language:
English
Imprint:
New York : Humana Press, c2012.
Format:
  • Book
  • xi, 256 p. : ill. (some col.) ; 26 cm.
Bibliography:
Includes bibliographical references and index.
Contents:
  • Isolation of fetal gonads from embryos of timed-pregnant mice for morphological and molecular studies
  • Neonatal testicular gonocytes isolation and processing for immunocytochemical analysis
  • Isolation of undifferentiated and early differentiating type a spermatogonia from pou5f1-GFP reporter mice
  • Isolation of human male germ-line stem cells using enzymatic digestion and magnetic-activated cell sorting
  • Isolation and purification of murine male germ cells
  • Preparation of enriched mouse syncitia-free pachytene spermatocyte cell suspensions
  • Revealing the transcriptome landscape of mouse spermatogonial cells by tiling microarray
  • Biochemical characterization of a testis-predominant isoform of n-[alpha] acetyltransferase
  • Identification of novel long noncoding RNA transcripts in male germ cells
  • Methylation profiling using methylated DNA immunoprecipitation and tiling array hybridization
  • Spermatogenesis in cryptorchidism
  • In vitro culture of fetal ovaries: A model to study factors regulating early follicular development
  • Microspread ovarian cell preparations for the analysis of meiotic prophase progression in oocytes with improved recovery by cytospin centrifugation
  • In vitro maturation (IVM) of porcine oocytes
  • Experimental approaches to the study of human primordial germ cells
  • Investigating the origins of somatic cell populations in the perinatal mouse ovaries using genetic lineage tracing and immunohistochemistry
  • DNA methylation analysis of germ cells by using bisulfite-based sequencing methods
  • Gene expression in mouse oocytes by RNA-Seq.
Contributor:
Chan, Wai-Yee.
Blomberg, Le Ann.
Series:
Methods in molecular biology, 1064-3745 ; 825
Springer protocols
Methods in molecular biology (Clifton, N.J.) v. 825.
Springer protocols (Series)
Subjects:
ISBN:
9781617794353
161779435X
9781617794360
1617794368

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