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Ras signaling : methods and protocols / edited by Lorenza Trabalzini, Saverio Francesco Retta.

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Language:
English.
Publication date:
2014
Copyright date:
2014
Publication:
New York : Humana Press, [2014]
Copyright notice:
©2014
Format:
  • Book
  • xii, 425 pages : illustrations (some color) ; 26 cm.
Bibliography:
Includes bibliographical references and index.
Contents:
  • The Ras superfamily of small GTPases : the unlocked secrets / Luca Goitre [and three others]
  • A novel method for the production of fully modified K-Ras 4B / Tanmay S. Chavan [and four others]
  • Metabolic labeling of Ras with tritiated palmitate to monitor palmitoylation and depalmitoylation / Frederick D. Tsai [and three others]
  • Ubiquitination of Rac1 by inhibitors of apoptosis (IAPs) / Tripat Kaur Oberoi-Khanuja and Krishnaraj Rajalingam
  • Ras GTPases are both regulators and effectors of redox agents / Elisa Ferro [and four others]
  • Biophysical and proteomic characterization strategies for cysteine modifications in Ras GTPases / G. Aaron Hobbs, Harsha P. Gunawardena, and Sharon L. Campbell
  • Use of the yeast two-hybrid technology to isolate molecular interactions of Ras GTPases / Elisa Ferro, Eva Baldini, and Lorenza Trabalzini
  • Screening for MAPK modulators using an In-Cell Western assay / Simon Schnaiter [and seven others]
  • Behavioral methods for the study of the Ras-ERK pathway in memory formation and consolidation : passive avoidance and novel object recognition tests / Raffaele d'Isa, Riccardo Brambilla, and Stefania Fasano
  • Functional phosphoproteomics of oncogenic KRAS signaling / Putty-Reddy Sudhir and Jeou-Yuan Chen
  • Pull-down assay for analysis of integrin-mediated activation of Rap proteins in adherent platelets / Gianni Francesco Guidetti and Mauro Torti
  • Combined pulldown and time-lapse microscopy studies for determining the role of Rap1 in the crosstalk between integrins and cadherins / Luca Goitre and Saverio Francesco Retta
  • Fluorescence microscopy study of Rap1 subcellular localization / Luca Goitre, Valentina Cutano, and Saverio Francesco Retta
  • An in vitro system to evaluate the scaffold function of the RalA effector protein RalBP1 / David F. Kashatus
  • Analysis of the Rit subfamily GTPase-mediated signaling and neuronal differentiation and survival / Geng-Xian Shi and Catherine N. Kaminski
  • Immunofluorescence methods in studies of the GTPase Ran and its effectors in interphase and in mitotic cells / Giulia Guarguaglini, Valeria de Turris, and Patrizia Lavia
  • High-resolution scanning electron microscopy for the imaging of nuclear pore complexes and Ran-mediated transport / Lihi Shaulov, Boris Fichtman, and Amnon Harel
  • Effector recruitment method to study spatially regulated activation of Ras and Rho GTPases / Lauren P. Huff, Molly J. DeCristo, and Adrienne D. Cox
  • Real-time visualization and quantification of native Ras activation in single living cells / Christoph Biskup and Ignacio Rubio
  • Nanoclustering and heterogeneous membrane diffusion of Ras studied by FRAP and RICS analysis / Camilo Guzmán, Maja Šolman, and Daniel Abankwa
  • Analyzing the roles of Rho GTPases in cancer cell migration with a live cell imaging 3D-morphology-based assay / Audrey Colomba and Anne J. Ridley
  • Analysis of Rho GTPase-induced localization of nanoscale adhesions using fluorescence nanoscopy / Annica K.B. Gad [and three others]
  • Yeast as a model for Ras signalling / Renata Tisi, Fiorella Belotti, and Enzo Martegani
  • Methods to study the Ras2 protein activation state and the subcellular localization of Ras-GTP in Saccharomyces cerevisiae / Sonia Colombo and Enzo Martegani
  • Ras proteins signaling in the early metazoan Dictyostelium discoideum / Enrico Bracco and Barbara Pergolizzi.
Contributor:
Trabalzini, Lorenza, editor.
Retta, Saverio Francesco, editor.
Springer Science+Business Media, copyright holder.
Series:
Methods in molecular biology ; 1120
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; v. 1120.
Springer protocols (Series)
Subjects:
ISBN:
9781627037907
162703790X

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