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Flower development : methods and protocols / edited by José Luis Riechmann, Frank Wellmer.

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Language:
English.
Publication date:
2014
Imprint:
New York : Humana Press : Springer, c2014.
Format:
  • Book
  • xiv, 475 p. : ill. (some col.) ; 26 cm.
Bibliography:
Includes bibliographical references and index.
Contents:
  • Flower Development in Arabidopsis - There is More to it than Learning your ABCs
  • Flower Development in the Asterid Lineage
  • Grass Flower Development
  • Flower Diversity and Angiosperm Diversification
  • Flower Development - Open Questions and Future Directions
  • Genetic Screens for Floral Mutants in Arabidopsis thaliana: Enhancers and Suppressors
  • Genetic and Phenotypic Analysis of Shoot Apical and Floral Meristem Development
  • Genetic and Phenotypic Analyses of Petal Development in Arabidopsis
  • Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsis and Rice
  • Molecular Cell Biology of Male Meiotic Chromosomes and Isolation of Male Meiocytes in Arabidopsis thaliana
  • Genetic and Phenotypic Analyses of Carpel Development in Arabidopsis
  • Microscopic Analysis of Arabidopsis Ovules
  • Scanning Electron Microscopy Analysis of Floral Development
  • Detection of mRNA Expression Patterns by Non-radioactive in situ Hybridization on Histological Sections of Floral Tissue
  • The GUS Reporter System in Flower Development Studies
  • A Floral Induction System for the Study of Early Arabidopsis Flower Development
  • Fluorescence Activated Cell Sorting of Shoot Apical Meristem Cell Types
  • Translating Ribosome Affinity Purification (TRAP) for Cell-specific Translation Profiling in Developing Flowers
  • Laser-assisted Microdissection Applied to Floral Tissues
  • Identification of Arabidopsis Knock-out Lines for Genes of Interest
  • Gene Expression Analysis by Quantitative Real-time PCR for Floral Tissues
  • Misexpression Approaches for the Manipulation of Flower Development
  • Next Generation Sequencing Applied to Flower Development: RNA-Seq
  • Next-Generation Sequencing Applied to Flower Development: ChIP-Seq
  • Live-Imaging of the Arabidopsis Inflorescence Meristem
  • Gene Regulatory Network Models for Floral Organ Determination.
Summary:
In Flower Development: Methods and Protocols, researchers in the field detail protocols for experimental approaches that are currently used to study the formation of flowers, from genetic methods and phenotypic analyses, to genome-wide experiments, modeling, and system-wide approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Flower Development: Methods and Protocols ?is an essential guide for plant developmental biologists, from the novice to the experienced researcher, and for those considering venturing into the field.
Contributor:
Riechmann, José Luis.
Wellmer, Frank.
Series:
Methods in Molecular Biology, 1064-3745 ; 1110
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; v. 1110.
Springer protocols (Series)
Subjects:
ISBN:
9781461494072
1461494079

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