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PIWI-interacting RNAs : methods and protocols / edited by Mikiko C. Siomi.

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Language:
English.
Publication date:
2014
Copyright date:
2014
Publication:
New York : Humana Press, [2014]
Copyright notice:
©2014
Format:
  • Book
  • xi, 249 pages : illustrations (some color) ; 26 cm.
Bibliography:
Includes bibliographical references and index.
Contents:
  • Chromatin Immunoprecipitation Assay of Piwi in Drosophila / Hang Yin and Haifan Lin
  • Drosophila Germline Stem Cells for In Vitro Analyses of PIWI-Mediated RNAi / Yuzo Niki, Takuya Sato, Takafumi Yamaguchi, Ayaka Saisho, Hiroshi Uetake, and Hidenori Watanabe
  • RNAi and Overexpression of Genes in Ovarian Somatic Cells / Kuniaki Saito
  • Making piRNAs In Vitro / Shinpei Kawaoka, Susumu Katsuma, and Yukihide Tomari
  • A Framework for piRNA Cluster Manipulation / Ivan Olovnikov, Adrien Le Thomas, and Alexei A. Aravin
  • Biochemical and Mass Spectrometric Analysis of 3ʹ-End Methylation of piRNAs / Takeo Suzuki, Kenjyo Miyauchi, Yuriko Sakaguchi, and Tsutomu Suzuki
  • HITS-CLIP (CLIP-Seq) for Mouse Piwi Proteins / Anastassios Vourekas and Zissimos Mourelatos
  • DNA Methylation in Mouse Testes / Satomi Kuramochi-Miyagawa, Kanako Kita-Kojima, Yusuke Shiromoto, Daisuke Ito, Hirotaka Koshima, and Toru Nakano
  • Analysis of Small RNA-Guided Endonuclease Activity in Endogenous Piwi Protein Complexes from Mouse Testes / Michael Reuter and Ramesh S. Pillai
  • Small RNA Library Construction from Minute Biological Samples / Jessica A. Matts, Yuliya Sytnikova, Gung-wei Chirn, Gabor L. Igloi, and Nelson C. Lau
  • Analysis of sDMA Modifications of PIWI Proteins / Shozo Honda, Yoriko Kirino, and Yohei Kirino
  • Analyses of piRNA-Mediated Transcriptional Transposon Silencing in Drosophila: Nuclear Run-On Assay on Ovaries / Sergey Shpiz and Alla Kalmykova
  • Combined RNA/DNA Fluorescence In Situ Hybridization on Whole-Mount Drosophila Ovaries / Shpiz, Sergey Lavrov, and Alla Kalmykova
  • Fast and Accurate Method to Purify Small Noncoding RNAs from Drosophila Ovaries / Thomas Grentzinger and Séverine Chambeyron
  • Isolation of Zebrafish Gonads for RNA Isolation / Hsin-Yi Huang and René F. Ketting
  • Small RNA Library Construction for High-Throughput Sequencing / Jon McGinn and Benjamin Czech
  • Analysis of Piwi-Loaded Small RNAs in Terahymena / Tomoko Noto, Henriette M. Kurth, and Kazufumi Mochizuki
  • Effective Gene Knockdown in the Drosophila Germline by Artificial miRNA-Mimicking siRNAs / Hailong Wang, Haidong Huang, and Dahua Chen
  • Isolation and Bioinformatic Analyses of Small RNAs Interacting with Germ Cell-Specific Argonaute in Rice / Reina Komiya and Ken-Ichi Nonomura.
Contributor:
Siomi, Mikiko C., editor.
Springer Science+Business Media, copyright holder.
Series:
Methods in molecular biology ; 1093
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; v. 1093.
Springer protocols (Series)
Subjects:
ISBN:
9781627036931
1627036938

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